FastQCFastQC Report
Thu 26 May 2016
SRR1364300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047522
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT140160.4599146454069896No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN89120.2924343122051293No Hit
TATCAACGCAGAGTACTTTTTTTTT87450.2869544502057737No Hit
GGTATCAACGCAGAGTACTTTTTTT58900.1932717794982284No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46970.1541252204249879No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33160.10880971490935916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.013.0642131
TATCACG1201.0120857E-811.86759952
GCGTTAT802.8877977E-511.8672091
TAACGCA1950.011.697354
GTATCAA79600.011.4736231
TTACGGC600.0059051811.07642653
TATAACG2250.010.9709372
GGACCGA1408.365532E-910.8568136
CGCATAT1855.456968E-1210.7829248
ATCACGC1701.4006218E-1010.6110313
ATAACGC2150.010.59804253
GCGCCAC1355.7934813E-810.54307513
GAACCGA1002.4012457E-510.4496826
CGCAGAT7900.010.3409478
CTCAACG1303.87814E-710.2243953
GTCAACG2151.8189894E-1210.1564583
TGTACCG750.0026397210.1375365
GCGTGCG1502.4518158E-810.1321919
CGTGCGC1502.4530891E-810.13185710
AGCCGAC750.002675302610.12118512
Produced by FastQC (version 0.11.5)