##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364300_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3047522
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.20277753532214	30.0	25.0	31.0	2.0	34.0
2	25.128990701297646	30.0	24.0	31.0	2.0	34.0
3	24.50427593303674	30.0	20.0	31.0	2.0	34.0
4	28.23632675990526	35.0	26.0	37.0	2.0	37.0
5	28.71208739428296	35.0	28.0	37.0	2.0	37.0
6	28.896023063984444	35.0	28.0	37.0	2.0	37.0
7	28.51577609612006	35.0	28.0	37.0	2.0	37.0
8	28.00503687914312	35.0	26.0	37.0	2.0	37.0
9	28.967831897521986	35.0	26.0	39.0	2.0	39.0
10	28.200107825308564	35.0	22.0	39.0	2.0	39.0
11	28.8570884804113	35.0	27.0	39.0	2.0	39.0
12	28.34818091551103	35.0	23.0	39.0	2.0	39.0
13	28.014965601560874	35.0	22.0	39.0	2.0	39.0
14	28.190145304939556	34.0	17.0	40.0	2.0	41.0
15	28.881908645778438	35.0	25.0	40.0	2.0	41.0
16	29.21078469655018	36.0	26.0	40.0	2.0	41.0
17	29.372721509475568	36.0	27.0	40.0	2.0	41.0
18	29.19988370879685	36.0	25.0	40.0	2.0	41.0
19	29.193654713567284	36.0	25.0	40.0	2.0	41.0
20	29.045519933900394	36.0	25.0	40.0	2.0	41.0
21	28.977744869438187	36.0	24.0	40.0	2.0	41.0
22	29.03928437596185	36.0	25.0	40.0	2.0	41.0
23	29.030720368876747	36.0	25.0	40.0	2.0	41.0
24	28.89021670721327	36.0	25.0	40.0	2.0	41.0
25	28.68347857702094	36.0	24.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6376662224433538
1101	2	0.7451989854141949
1101	3	1.4838337189735107
1101	4	0.9147903185862134
1101	5	0.3914340145460464
1101	6	0.30241859566180196
1101	7	0.5886276890531299
1101	8	0.7501509782149363
1101	9	0.6789197527541084
1101	10	0.976887656389497
1101	11	0.4941638587308219
1101	12	0.8844169653271479
1101	13	0.6833632847496958
1101	14	1.2789731308995833
1101	15	0.5188470221075931
1101	16	-0.015210404886044415
1101	17	-0.6256039134433955
1101	18	-6.3653764804533
1101	19	-7.855448033500309
1101	20	-9.054773453741284
1101	21	-10.627260578932436
1101	22	-10.970119315453672
1101	23	-12.147110519947805
1101	24	-11.886423901870828
1101	25	-12.029849693864385
1102	1	1.953102718452886
1102	2	2.251700603606647
1102	3	2.9526482923664545
1102	4	2.2885313890084475
1102	5	1.8763775119886041
1102	6	1.7690775917920938
1102	7	2.062197547736357
1102	8	2.3465756584676214
1102	9	2.4965705291107874
1102	10	-0.998165209931166
1102	11	1.171783961887236
1102	12	2.1443756920270545
1102	13	2.6464417281719967
1102	14	3.153069438844561
1102	15	2.851700555563653
1102	16	2.4747828996078596
1102	17	2.217447184404918
1102	18	2.309488267640024
1102	19	2.002840036959494
1102	20	2.390104349918033
1102	21	2.543719702198313
1102	22	2.353203856096197
1102	23	2.40588025125642
1102	24	2.559805079070461
1102	25	2.603771273401435
1103	1	-1.9856915244609041
1103	2	-1.7279586708829981
1103	3	-3.644998204916554
1103	4	-1.5451538070555841
1103	5	-4.637749011730001
1103	6	-6.728765336684923
1103	7	-9.537844675791327
1103	8	-4.099818819429281
1103	9	-9.036272032205115
1103	10	-6.425237834398786
1103	11	-10.125607358424116
1103	12	-3.757647365690673
1103	13	-1.6255879384457153
1103	14	-0.9086778808793348
1103	15	-1.6969299334949461
1103	16	-1.8448124888485928
1103	17	-1.4637192864424868
1103	18	-1.2933627383814184
1103	19	-7.42017545723936
1103	20	-3.906121233868131
1103	21	-7.836393016262548
1103	22	-4.289263833726096
1103	23	-2.8329305139073355
1103	24	-7.782490045029995
1103	25	-4.898431693260335
1104	1	1.535913583450128
1104	2	1.6917584519780178
1104	3	2.513057564384681
1104	4	1.971639732939284
1104	5	1.544506585353286
1104	6	1.4401250484237238
1104	7	1.5800673648081371
1104	8	1.9544481550311659
1104	9	2.1714704470004804
1104	10	2.588578533634635
1104	11	1.958827528534954
1104	12	2.1998615200225657
1104	13	2.47332287464285
1104	14	2.9580616119937737
1104	15	2.061616840166703
1104	16	1.2538509899539427
1104	17	0.9444716006631495
1104	18	1.088193910330613
1104	19	1.580028364533387
1104	20	-8.774429467351698
1104	21	-3.174959301851203
1104	22	-0.7758367881799444
1104	23	0.26746659502967773
1104	24	0.9343373889225894
1104	25	1.3476796621315508
1105	1	1.3605480768281595
1105	2	1.3100949902127077
1105	3	2.1504001312896968
1105	4	1.6302799409770898
1105	5	1.3768794451387691
1105	6	1.1474010052171977
1105	7	1.4542626089272517
1105	8	1.6492068869336052
1105	9	1.7474909896040707
1105	10	2.2651850465125563
1105	11	1.7377151702425984
1105	12	1.989551540102628
1105	13	2.031470069498525
1105	14	2.7215542633161256
1105	15	1.9908089558103086
1105	16	1.5468441850628203
1105	17	0.879658327287558
1105	18	1.455742597749886
1105	19	1.4473815014655784
1105	20	1.3468578562915035
1105	21	1.2743157823584532
1105	22	1.2946706262903902
1105	23	1.2488492582578452
1105	24	1.3202557767492173
1105	25	1.3274451710130641
1106	1	0.8757555147384259
1106	2	1.1730004617275114
1106	3	1.70070654904508
1106	4	1.2937223397334066
1106	5	0.5917651667520651
1106	6	0.35917252820248535
1106	7	0.8949639147007495
1106	8	0.7840427318634653
1106	9	1.1547204615396716
1106	10	-2.574893797701538
1106	11	-0.30674789220226373
1106	12	0.3629739854352678
1106	13	1.3567932125134199
1106	14	0.3940222859263365
1106	15	1.105405345904554
1106	16	0.7195119481822658
1106	17	-5.829284340654333
1106	18	-1.9784649068551445
1106	19	-0.5677463496497026
1106	20	0.2873733449475466
1106	21	0.5924539395339501
1106	22	0.6691911287426286
1106	23	0.1326136895955834
1106	24	0.4543218815755097
1106	25	0.5610136778822827
1107	1	0.5301688517663301
1107	2	0.952510488976106
1107	3	1.4649096119162444
1107	4	0.8390598255607671
1107	5	0.2340517452212083
1107	6	0.562899768613736
1107	7	0.4860020977978188
1107	8	0.5422406874282473
1107	9	0.7472848890269894
1107	10	-2.4380301224899164
1107	11	-0.2657885395677866
1107	12	0.16576753350740958
1107	13	0.9772656177260615
1107	14	1.3431536037942777
1107	15	1.0761345947220988
1107	16	-5.559709813288375
1107	17	-2.2155601393241504
1107	18	-0.47954182104648524
1107	19	0.3503081296601387
1107	20	0.7287622256237363
1107	21	0.8503668953015691
1107	22	0.4372922256308698
1107	23	0.17073713954391323
1107	24	0.5071890001622421
1107	25	0.6895638849454642
1108	1	-0.7574889150686204
1108	2	-3.4785022942759696
1108	3	-0.5124303950512186
1108	4	-1.0479887553636758
1108	5	-1.3061172831983576
1108	6	-0.9023695597803325
1108	7	-0.5347522216655136
1108	8	-0.23288256642504734
1108	9	-0.7432685606452054
1108	10	-3.7363644700375893
1108	11	-1.8431067861889652
1108	12	-6.238453645017724
1108	13	-2.317281945236239
1108	14	-6.12336858778049
1108	15	-3.033118084412429
1108	16	-7.939503100377227
1108	17	-3.79191283544489
1108	18	-2.3990378804864783
1108	19	-1.1063831595244835
1108	20	-1.048092774706646
1108	21	-0.5326577513176716
1108	22	-0.7142795701927334
1108	23	-0.6633939290067126
1108	24	-0.46184523138790823
1108	25	-0.7596122143758137
1109	1	-7.975349338556921
1109	2	-5.964730887430495
1109	3	-5.2298807381136925
1109	4	-5.034211519006554
1109	5	-2.392256161883221
1109	6	-1.500790362221185
1109	7	-1.012372254953739
1109	8	-0.5948137121769825
1109	9	-5.408795014840461
1109	10	-1.487788906921768
1109	11	-1.1424485228776398
1109	12	-5.786784240052267
1109	13	-7.674438806678605
1109	14	-7.755677370154103
1109	15	-3.851373242994434
1109	16	-2.1935101395125365
1109	17	-1.8713418604976475
1109	18	-1.1718484380749814
1109	19	-0.8795600333374196
1109	20	-0.6214429907629224
1109	21	-0.6748123216762352
1109	22	-0.8586841605902436
1109	23	-1.1917277559910922
1109	24	-0.7703986252138542
1109	25	-0.6262917545473634
1110	1	-2.0876501349560996
1110	2	-2.0174080426494427
1110	3	-1.1925961354413133
1110	4	-1.9811571329979358
1110	5	-5.284561650310934
1110	6	-3.882377679626533
1110	7	-8.591124424497906
1110	8	-9.409205367840219
1110	9	-11.285773140852566
1110	10	-8.94148875827166
1110	11	-11.59272672279958
1110	12	-6.625385724892446
1110	13	-4.679523636893606
1110	14	-4.214082537198625
1110	15	-9.554912195135742
1110	16	-11.060167256032631
1110	17	-12.110882996715954
1110	18	-8.92339784555067
1110	19	-12.08736873144483
1110	20	-13.098323746802352
1110	21	-13.644070399854565
1110	22	-14.452576841856178
1110	23	-15.085138570078804
1110	24	-15.469620495879957
1110	25	-16.115634206194557
1111	1	-0.6952101804081323
1111	2	-0.029752115533586476
1111	3	-2.8160969474438673
1111	4	-3.38955444609908
1111	5	-1.1840410133791472
1111	6	-0.6767786329125052
1111	7	-0.18813585286821777
1111	8	0.149614287146683
1111	9	-4.326830679906621
1111	10	-4.1532191784515575
1111	11	-1.443415542303729
1111	12	-5.856621803950823
1111	13	-1.4012269819418997
1111	14	-0.1417870336313456
1111	15	-0.020368399790555713
1111	16	-0.03536598297351645
1111	17	-0.03145016667336975
1111	18	0.1301710690809621
1111	19	0.24753480868519162
1111	20	0.2652867232541105
1111	21	0.3398979546712795
1111	22	0.005301719768027624
1111	23	-0.12484197546165277
1111	24	0.09968631036064579
1111	25	0.26867302741581156
1112	1	-0.5740937225951335
1112	2	0.08164966843036936
1112	3	-2.671606204312255
1112	4	-0.7337066075994549
1112	5	-0.7726750796379989
1112	6	-0.47424962493510137
1112	7	-0.14180046761064347
1112	8	-3.8865016007111457
1112	9	-1.0622946178454846
1112	10	-3.409679519635027
1112	11	-1.009121823809167
1112	12	0.08574973367859329
1112	13	0.606769390556817
1112	14	1.2787607450979301
1112	15	0.811454702226623
1112	16	0.488377638431686
1112	17	0.1738175379711535
1112	18	0.24527220893134327
1112	19	0.3930940785841841
1112	20	0.3328031155672271
1112	21	0.26668937600243936
1112	22	0.3088614259332303
1112	23	0.05992224509104105
1112	24	0.3174858774687799
1112	25	0.31291683565154926
1113	1	-1.368149524146883
1113	2	-0.8126658462637479
1113	3	-3.275984230980299
1113	4	-1.6798786461198034
1113	5	-1.926560049569538
1113	6	-1.5654645830344265
1113	7	-1.2664814319539914
1113	8	-8.552911926667466
1113	9	-9.575171388461985
1113	10	-3.8922754384182525
1113	11	-9.475004830554749
1113	12	-7.879428650036722
1113	13	-3.3290539456681962
1113	14	-1.6506605312169071
1113	15	-7.525317435457577
1113	16	-3.394290428870576
1113	17	-2.458685136654452
1113	18	-1.796080983003808
1113	19	-1.7128921317565506
1113	20	-6.723596504711278
1113	21	-2.994335785928115
1113	22	-2.325209429581019
1113	23	-2.294060597122911
1113	24	-8.009007159176644
1113	25	-5.213532179992946
1114	1	-4.395591698913318
1114	2	-4.200976697632367
1114	3	-0.9112901219547389
1114	4	-0.7959654953672732
1114	5	-0.7412598665468266
1114	6	-0.8249782655471058
1114	7	-0.6989442105848909
1114	8	-0.33412779403676396
1114	9	-0.5692629219124257
1114	10	-3.4919537677191954
1114	11	-1.4409082888369866
1114	12	-4.677714239115755
1114	13	-1.4694191072768845
1114	14	-5.8771916432363795
1114	15	-2.2948752717387926
1114	16	-1.4633577495580354
1114	17	-1.2078775535485171
1114	18	-0.8622302514653484
1114	19	-0.5617702430505638
1114	20	-0.3878231140774737
1114	21	-0.13388526282195912
1114	22	-0.2786828102719383
1114	23	-0.6726305526745371
1114	24	-0.5307382520468593
1114	25	-0.4595045756018763
1115	1	0.39018470774062664
1115	2	0.431734647846465
1115	3	1.1302482353901269
1115	4	-0.07139969971714066
1115	5	-0.24134977843317884
1115	6	-0.7151152819204079
1115	7	-2.756012802844996
1115	8	-0.6538166288487766
1115	9	-0.29070795772538105
1115	10	0.4284176968169895
1115	11	-0.43925741318862777
1115	12	0.34227048754043565
1115	13	0.44618095531113156
1115	14	1.0180061278829875
1115	15	0.1779879197361609
1115	16	-0.13432508468649118
1115	17	-0.4730155624556325
1115	18	-0.0460598906267613
1115	19	0.21168379796326775
1115	20	0.25499596218882203
1115	21	0.25414641418652195
1115	22	-0.15989636384657757
1115	23	-0.26785658181533023
1115	24	-0.37656178654149386
1115	25	-0.14807572392846424
1116	1	-0.07522431214481884
1116	2	0.2194982130690626
1116	3	1.0811052735984994
1116	4	0.34581983612866907
1116	5	-0.5620706038570589
1116	6	-1.5492430235211287
1116	7	-4.839931054043301
1116	8	-1.4326649657802513
1116	9	-1.4538887134873946
1116	10	-0.6802497704798505
1116	11	-2.368634494739034
1116	12	-1.0239315905761828
1116	13	-6.768906168982593
1116	14	-2.177123997999086
1116	15	-1.7495217682411202
1116	16	-1.1451998024995724
1116	17	-0.8164243921620624
1116	18	-0.5976292783794683
1116	19	-0.3288948862562151
1116	20	-0.23908917696469345
1116	21	-0.09979674811483008
1116	22	-0.4204937372630724
1116	23	-0.4326394196853727
1116	24	-0.25031743468214174
1116	25	-0.4651884895187415
1201	1	2.389122681361883
1201	2	2.598778969159362
1201	3	3.2901038889838254
1201	4	2.877138939539737
1201	5	2.345412229746689
1201	6	2.2301544095202317
1201	7	2.5205353383290294
1201	8	2.547116505617634
1201	9	2.9878161828702225
1201	10	3.1572420069329894
1201	11	2.794585913968728
1201	12	3.1197911828852156
1201	13	3.279487493990235
1201	14	3.8615121401154084
1201	15	3.3376463628839232
1201	16	2.8154651805492463
1201	17	2.6918961103209753
1201	18	2.6436123930679614
1201	19	2.652513030953422
1201	20	2.9374493586295003
1201	21	2.711940177725154
1201	22	1.8247894055953822
1201	23	1.245747514073269
1201	24	1.8224592796081716
1201	25	1.969433810334781
1202	1	2.178980449311485
1202	2	2.402527300607158
1202	3	3.163293921367206
1202	4	2.6231418965434656
1202	5	2.093396498965717
1202	6	1.9304127411647052
1202	7	2.257194402982062
1202	8	2.6361481424791045
1202	9	2.8188693396726556
1202	10	-1.0184619916919786
1202	11	1.381482744638788
1202	12	2.3323311704175715
1202	13	2.9085844790949054
1202	14	3.4168046038604736
1202	15	2.944976277448923
1202	16	2.6764227264242777
1202	17	2.3099192960350052
1202	18	2.4561470788585247
1202	19	2.616379950393455
1202	20	2.584786045742078
1202	21	2.6920817342310315
1202	22	2.383865773765436
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2211	17	-0.14128050231639833
2211	18	0.42706717999741883
2211	19	0.6709095413533781
2211	20	0.771527935847601
2211	21	0.7559409268840902
2211	22	0.6925473117847396
2211	23	0.5817190779868397
2211	24	0.8083486828056365
2211	25	0.8411369810993605
2212	1	1.173377370611476
2212	2	1.2319229817533994
2212	3	1.9192827291822816
2212	4	1.4131450376487216
2212	5	1.0583584695140544
2212	6	0.8625466107632143
2212	7	1.3094022510516865
2212	8	1.568297837257699
2212	9	1.5529287708662807
2212	10	2.1544639196782107
2212	11	1.3274433442633544
2212	12	1.6204962361549846
2212	13	1.7190239103796188
2212	14	-3.966303977177457
2212	15	-0.6543636272711417
2212	16	0.07463821736111953
2212	17	0.41667194669477325
2212	18	0.7263199955835731
2212	19	1.3285235142656937
2212	20	1.5854253830175011
2212	21	1.432950701817127
2212	22	1.3083969566652236
2212	23	1.3262638610706503
2212	24	1.3903001877902668
2212	25	1.4072857876314941
2213	1	0.9476071232462715
2213	2	1.0335769049579433
2213	3	1.6750861124167322
2213	4	1.2723190399048825
2213	5	0.777539533974636
2213	6	0.4969029942963381
2213	7	0.7559250860836606
2213	8	-4.793293158020539
2213	9	-1.437288484803414
2213	10	0.5182599504848326
2213	11	0.31810925852152394
2213	12	0.975696834813089
2213	13	-7.179538973655347
2213	14	-1.8620052447122006
2213	15	-0.5042862635472858
2213	16	-0.011524559446495886
2213	17	0.21991989074895812
2213	18	0.46052822966017004
2213	19	0.7356787470158075
2213	20	1.0213069088282367
2213	21	1.0919673956776457
2213	22	0.78291390256787
2213	23	0.6520627424923866
2213	24	0.805390008204256
2213	25	0.9640278897975953
2214	1	-1.0469740706910962
2214	2	-0.861616364750116
2214	3	-6.663719448768688
2214	4	-2.67162687102865
2214	5	-1.6701112466445913
2214	6	-1.2423402216603314
2214	7	-0.7790404467645864
2214	8	-6.337465795296151
2214	9	-2.1708728395807917
2214	10	-0.3035442405193258
2214	11	-0.7306091653238305
2214	12	-0.37228633831393765
2214	13	-6.086993001290569
2214	14	-1.8291665811455218
2214	15	-0.9962935522302914
2214	16	-0.9272754253691211
2214	17	-0.7741854224976308
2214	18	-0.7236285289220774
2214	19	-0.3858212255683746
2214	20	-0.07897543028253651
2214	21	-0.040485644111718955
2214	22	-0.2216736650233635
2214	23	-0.3486428290306449
2214	24	-0.05677411610725969
2214	25	0.030881067858707922
2215	1	0.38672293568969707
2215	2	0.7481392399253153
2215	3	-2.422302071902795
2215	4	-0.5008351534235622
2215	5	0.07277314464797868
2215	6	-0.40757924386970146
2215	7	0.22058545801507634
2215	8	0.5995225284484995
2215	9	0.8322523228253615
2215	10	1.5230714207015232
2215	11	0.7195964179738681
2215	12	0.3755716314218276
2215	13	1.058568080955272
2215	14	1.0278855686460204
2215	15	0.89889545763873
2215	16	0.5963452479547726
2215	17	0.4838610162732735
2215	18	0.5615839938359173
2215	19	0.6794822418188993
2215	20	0.9375668405652178
2215	21	1.0471763026269905
2215	22	0.7500370797054998
2215	23	0.6092050570817769
2215	24	0.47089168067630993
2215	25	0.6075721378680541
2216	1	-0.13913513431399238
2216	2	-0.39779187534560734
2216	3	0.9737801006175246
2216	4	-0.5343963233485276
2216	5	-0.7989483379202156
2216	6	-1.0016458735235396
2216	7	-1.5471054180866979
2216	8	-6.524053879630319
2216	9	-2.4719176745336284
2216	10	-1.0153364205028375
2216	11	-1.728182471646253
2216	12	-0.6026573071465435
2216	13	0.13769771271964615
2216	14	0.7797361807576912
2216	15	0.33585477875513803
2216	16	-0.45879956495596375
2216	17	-0.5765368607272556
2216	18	-0.25533805043266966
2216	19	-0.07857184517705917
2216	20	-0.1418552446932715
2216	21	-0.1904400172566909
2216	22	-0.46108947163618197
2216	23	-0.7944170377115967
2216	24	-0.7290975232140227
2216	25	-0.629639580148659
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	364311.0
3	7.0
4	2663.0
5	15807.0
6	22524.0
7	20379.0
8	17988.0
9	14662.0
10	13060.0
11	11805.0
12	11048.0
13	10526.0
14	10564.0
15	10947.0
16	11042.0
17	11934.0
18	13551.0
19	17837.0
20	24376.0
21	30726.0
22	37119.0
23	43997.0
24	49769.0
25	55324.0
26	61034.0
27	67040.0
28	71852.0
29	83117.0
30	93692.0
31	105160.0
32	127692.0
33	158287.0
34	195158.0
35	237779.0
36	289105.0
37	329118.0
38	406522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.916401793017876	18.53284586922245	13.665306842894909	22.88544549486477
2	14.182657159529946	20.718791699653163	42.01852995920432	23.08002118161257
3	16.676497895590042	27.401561732707165	29.983206219001573	25.938734152701215
4	10.575993897590985	17.00862070669111	38.774999357745166	33.64038603797274
5	12.39925769870789	39.27734320960327	33.93100181688584	14.392397274802995
6	29.95548831510665	37.988626181508394	18.46760904243588	13.588276460949078
7	27.644201646589096	33.42049782193029	21.516339473365395	17.41896105811522
8	25.438556929029936	35.55360374344102	20.731536693208167	18.276302634320867
9	26.70030918902405	16.852019868160113	20.249115756262132	36.1985551865537
10	13.272848199848788	31.40441391184437	33.312470708281175	22.010267180025668
11	33.301378544092834	25.217925188263855	22.8171350973211	18.66356117032221
12	22.50339981979556	27.889089664712426	30.133316390472803	19.474194125019217
13	27.30347845351007	23.425962836497774	27.008238988265802	22.262319721726353
14	20.974225032557783	22.513724307661516	29.460054331008312	27.051996328772393
15	23.04283852109338	30.41097198082899	24.285497972296834	22.2606915257808
16	21.916622692682587	30.305245835693885	26.000755156477705	21.777376315145823
17	21.342981388662956	29.799107106119944	26.708935884189106	22.148975621028004
18	20.539759251889517	28.639209308128155	29.613189433522045	21.207842006460282
19	22.12087297146055	27.912143256855064	29.288093748971328	20.67889002271306
20	22.2806812694442	27.532199203628792	29.396071974224803	20.7910475527022
21	23.120670704791074	26.870062961691367	29.06534460391609	20.943921729601474
22	22.329104740222483	27.401737632788876	28.7426373793498	21.526520247638842
23	22.03039740193821	27.58992309634402	28.728352639619324	21.651326862098443
24	21.836415257375634	27.448002304242276	29.06962926387689	21.645953174505205
25	21.823514754612063	27.346368595557635	29.304455760850566	21.525660888979736
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12477.0
1	12477.0
2	9451.5
3	6426.0
4	6426.0
5	6426.0
6	10508.0
7	14590.0
8	14590.0
9	14590.0
10	16707.0
11	18824.0
12	18824.0
13	18824.0
14	23499.5
15	28175.0
16	28175.0
17	28175.0
18	41799.0
19	55423.0
20	55423.0
21	55423.0
22	77089.0
23	98755.0
24	98755.0
25	98755.0
26	132677.5
27	166600.0
28	166600.0
29	166600.0
30	196518.0
31	226436.0
32	226436.0
33	226436.0
34	251182.0
35	275928.0
36	275928.0
37	275928.0
38	299144.5
39	322361.0
40	322361.0
41	322361.0
42	341766.5
43	361172.0
44	361172.0
45	361172.0
46	370927.5
47	380683.0
48	380683.0
49	380683.0
50	365013.0
51	349343.0
52	349343.0
53	349343.0
54	314388.0
55	279433.0
56	279433.0
57	279433.0
58	239781.0
59	200129.0
60	200129.0
61	200129.0
62	162850.0
63	125571.0
64	125571.0
65	125571.0
66	96281.5
67	66992.0
68	66992.0
69	66992.0
70	49944.5
71	32897.0
72	32897.0
73	32897.0
74	23582.5
75	14268.0
76	14268.0
77	14268.0
78	10552.0
79	6836.0
80	6836.0
81	6836.0
82	4699.5
83	2563.0
84	2563.0
85	2563.0
86	1826.0
87	1089.0
88	1089.0
89	1089.0
90	762.0
91	435.0
92	435.0
93	435.0
94	255.5
95	76.0
96	76.0
97	76.0
98	58.0
99	40.0
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3561582164132039
2	0.358520791646459
3	0.37010397299839015
4	0.372236853417301
5	0.3792589520272536
6	0.31724135215430765
7	0.32715104271601647
8	0.32449314557860454
9	0.3247884674827614
10	0.43930773920582034
11	0.3247556539378551
12	0.32357436632122755
13	0.3166178948010876
14	0.3230821631476327
15	0.3150756581904905
16	0.3196367409324691
17	0.312877150681767
18	0.31418969247801987
19	0.3157319290886169
20	0.5174696031726761
21	0.31642101353164964
22	0.3186523345852794
23	0.31386155702895663
24	0.3165522677112749
25	0.3102520670892614
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3047522.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.67064756665418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.61631436320457	58.41436415998393
2	7.032234785489319	9.376872939786487
3	2.242064775434106	4.484397313937309
4	1.0028790383882054	2.6745037968146037
5	0.5410350734703276	1.8035579352269535
6	0.3252426777407672	1.3010483964773745
7	0.20905137310314875	0.9756313293639611
8	0.142965318805502	0.7625272307468787
9	0.11376498825827586	0.6826306893832855
>10	0.6656210377705783	8.458626052324313
>50	0.06452101618985358	2.9965966582231105
>100	0.041316661605767045	5.0031893481813725
>500	0.0020922233779412064	0.9277073872056973
>1k	6.974077924640787E-4	0.8908089905973481
>5k	1.494445269565883E-4	0.782040090283937
>10k+	4.981484231886276E-5	0.4654976814634416
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	14016	0.4599146454069896	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	8912	0.2924343122051293	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8745	0.2869544502057737	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5890	0.1932717794982284	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4697	0.1541252204249879	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3316	0.10880971490935916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.281354490632061E-5	0.0
4	0.0	0.0	0.0	3.281354490632061E-5	0.0
5	0.0	0.0	0.0	3.281354490632061E-5	0.0
6	0.0	0.0	0.0	3.281354490632061E-5	0.0
7	0.0	0.0	0.0	3.281354490632061E-5	0.0
8	0.0	0.0	0.0	3.281354490632061E-5	0.0
9	0.0	0.0	0.0	3.281354490632061E-5	0.0
10	0.0	0.0	0.0	3.281354490632061E-5	0.0
11	0.0	0.0	0.0	3.281354490632061E-5	0.0
12	0.0	0.0	0.0	3.281354490632061E-5	0.0
13	0.0	0.0	0.0	3.281354490632061E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2885	0.0	13.064213	1
TATCACG	120	1.0120857E-8	11.8675995	2
GCGTTAT	80	2.8877977E-5	11.867209	1
TAACGCA	195	0.0	11.69735	4
GTATCAA	7960	0.0	11.473623	1
TTACGGC	60	0.00590518	11.0764265	3
TATAACG	225	0.0	10.970937	2
GGACCGA	140	8.365532E-9	10.856813	6
CGCATAT	185	5.456968E-12	10.782924	8
ATCACGC	170	1.4006218E-10	10.611031	3
ATAACGC	215	0.0	10.5980425	3
GCGCCAC	135	5.7934813E-8	10.543075	13
GAACCGA	100	2.4012457E-5	10.449682	6
CGCAGAT	790	0.0	10.340947	8
CTCAACG	130	3.87814E-7	10.224395	3
GTCAACG	215	1.8189894E-12	10.156458	3
TGTACCG	75	0.00263972	10.137536	5
GCGTGCG	150	2.4518158E-8	10.132191	9
CGTGCGC	150	2.4530891E-8	10.131857	10
AGCCGAC	75	0.0026753026	10.121185	12
>>END_MODULE