FastQCFastQC Report
Thu 26 May 2016
SRR1364300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047522
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT300560.9862439057043723No Hit
TATCAACGCAGAGTACTTTTTTTTT231170.7585507175994136No Hit
GGTATCAACGCAGAGTACTTTTTTT202790.6654258771552757No Hit
ACGCAGAGTACTTTTTTTTTTTTTT102340.3358138185712851No Hit
GTACTTTTTTTTTTTTTTTTTTTTT95370.31294277777157964No Hit
GAGTACTTTTTTTTTTTTTTTTTTT72830.23898104755273303No Hit
GTGGTATCAACGCAGAGTACATGGG45240.14844847715619444No Hit
GTGGTATCAACGCAGAGTACTTTTT37110.12177106514735579No Hit
ATCAACGCAGAGTACTTTTTTTTTT34850.11435520399852733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1650.013.8199012
GGTATCA52650.013.7352081
TAACGCA3850.013.5740094
CGTATCA707.25869E-613.5731172
TATAACG3550.013.1143072
ATAACGC4000.013.0647693
ATCACGC1850.012.8400673
TGGTATC14500.012.5808752
GCGTATC701.0886272E-412.2178121
GTGGTAT15950.011.9156641
CAACCGT2350.011.7239016
GCCCCCG2300.011.56191218
TAAACGC1900.011.5021874
TATGACG752.0714008E-411.4021674
AACCGTC752.0742453E-411.4004837
TGCACGG2550.011.17254212
TTGACCG2050.011.118772518
CGAAATC1201.2809323E-711.0807113
ATTCGAT600.005888939411.08052816
TTGCGCG2250.010.97463918
Produced by FastQC (version 0.11.5)