##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364300_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3047522
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.684148629607925	31.0	30.0	34.0	26.0	34.0
2	29.708761085235807	31.0	30.0	34.0	26.0	34.0
3	29.744273544210674	31.0	30.0	34.0	26.0	34.0
4	33.09694302452944	35.0	33.0	37.0	27.0	37.0
5	32.705954870875416	35.0	33.0	37.0	26.0	37.0
6	32.61948396106739	35.0	33.0	37.0	25.0	37.0
7	32.341526656739475	35.0	32.0	37.0	23.0	37.0
8	32.22296081865857	35.0	32.0	37.0	22.0	37.0
9	32.9539550493811	37.0	32.0	39.0	17.0	39.0
10	33.0798268232354	37.0	32.0	39.0	17.0	39.0
11	33.034290154427104	37.0	32.0	39.0	17.0	39.0
12	32.959741718025334	37.0	32.0	39.0	17.0	39.0
13	33.032500175552464	37.0	32.0	39.0	17.0	39.0
14	33.95343101706895	38.0	33.0	41.0	17.0	41.0
15	33.9912955509427	38.0	33.0	41.0	17.0	41.0
16	33.944736083939674	38.0	33.0	41.0	17.0	41.0
17	33.930288608252866	38.0	33.0	41.0	17.0	41.0
18	33.88037100306413	38.0	33.0	41.0	17.0	41.0
19	33.80574282974823	39.0	33.0	41.0	17.0	41.0
20	33.690685415888716	39.0	33.0	41.0	12.0	41.0
21	33.630709146644385	39.0	33.0	41.0	10.0	41.0
22	33.60198023180801	39.0	33.0	41.0	10.0	41.0
23	33.33436214734463	38.0	32.0	41.0	2.0	41.0
24	33.25705179486809	38.0	32.0	41.0	2.0	41.0
25	33.102493107514896	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.40667853783707386
1101	2	0.590796128337157
1101	3	-1.4042681472546867
1101	4	-0.38705007969483063
1101	5	0.4970857474908854
1101	6	0.7926445826178465
1101	7	0.9894918621730753
1101	8	1.0117520412527057
1101	9	1.8310530729792305
1101	10	1.5261772198488899
1101	11	1.4650943912546026
1101	12	1.6003162999311797
1101	13	1.3950750077934941
1101	14	1.5131625443640715
1101	15	1.570740892224073
1101	16	1.5475691276797576
1101	17	1.523702858846974
1101	18	1.3508104794568752
1101	19	1.4614117255947718
1101	20	1.7716748925373267
1101	21	1.5311994561859663
1101	22	1.7004277763668725
1101	23	1.9869581355686634
1101	24	1.9750259923481437
1101	25	2.122056194727797
1102	1	0.744895185092588
1102	2	0.7716908572697925
1102	3	0.905355401605064
1102	4	0.8139771236499698
1102	5	0.9107606813408964
1102	6	1.057301568754525
1102	7	1.151420463120914
1102	8	1.2620807785258705
1102	9	1.9015484330874344
1102	10	1.4771229097677931
1102	11	1.4504863802627028
1102	12	1.6120673156622658
1102	13	1.4944039812207848
1102	14	1.542879052073964
1102	15	1.6122540363478564
1102	16	1.616882710835526
1102	17	1.5919265773083282
1102	18	1.5632062898680559
1102	19	1.5130784906589838
1102	20	1.748304020538228
1102	21	1.9059532386772844
1102	22	1.8980901189084278
1102	23	2.131452255867721
1102	24	2.0926133022843274
1102	25	2.0113069555197782
1103	1	-3.049981081613243
1103	2	-3.0283899181744722
1103	3	-2.8936973227303717
1103	4	-3.183939237049106
1103	5	-6.009464388419715
1103	6	-9.167318419496322
1103	7	-10.927904694758364
1103	8	-12.215917091939687
1103	9	-12.539586461904491
1103	10	-11.140992819414173
1103	11	-12.543079121279373
1103	12	-12.39326569946477
1103	13	-12.93637697046081
1103	14	-13.447253493086851
1103	15	-13.27932328778197
1103	16	-7.997451258910399
1103	17	-6.481790194611193
1103	18	-5.200911054822797
1103	19	-4.633914459847695
1103	20	-9.503430815707915
1103	21	-6.094744367432277
1103	22	-12.51744446766816
1103	23	-12.54620782637637
1103	24	-13.05023072767602
1103	25	-14.182912092798627
1104	1	0.292318119588618
1104	2	0.5334299125389492
1104	3	0.5738469226252398
1104	4	0.6703968994061356
1104	5	0.7209068878252012
1104	6	0.6927247265957988
1104	7	0.5976211196126755
1104	8	0.8788325507996788
1104	9	1.1419683547017172
1104	10	0.969890805223983
1104	11	0.8589146678818906
1104	12	0.9306116776925322
1104	13	0.7645477849530593
1104	14	0.838859602805563
1104	15	0.836062736914819
1104	16	0.5048342931030874
1104	17	0.555925993875853
1104	18	0.7897085633663465
1104	19	0.8504576774869577
1104	20	1.1835241328099144
1104	21	0.9844026040968004
1104	22	0.7516231736788015
1104	23	1.0977888208699724
1104	24	1.3216317911030657
1104	25	1.4233477547701696
1105	1	0.4377793545705231
1105	2	0.5904270645130794
1105	3	0.17214244343915297
1105	4	0.5636578175862823
1105	5	0.737954670262738
1105	6	0.6641240971790907
1105	7	0.9132760741841537
1105	8	0.8009878394850318
1105	9	0.972521946999052
1105	10	0.48636796901421775
1105	11	1.034737898881012
1105	12	1.2367228713214118
1105	13	1.089924273637216
1105	14	0.8828306334214275
1105	15	0.7040949839710109
1105	16	0.9773201568131995
1105	17	0.9532928077121241
1105	18	1.0419691070598347
1105	19	1.1139121158849008
1105	20	1.321341536836492
1105	21	1.4045811215881798
1105	22	1.0911424318297023
1105	23	1.6142180523615437
1105	24	1.5409032002262535
1105	25	1.6270397546119995
1106	1	-0.09673879637554705
1106	2	0.08597622027423668
1106	3	-0.03610766468199955
1106	4	-1.3599713471408492
1106	5	-0.29423294555066093
1106	6	-0.032466459529679526
1106	7	0.28049814093805026
1106	8	0.3950608063088552
1106	9	0.7969961069028457
1106	10	0.4621139362155162
1106	11	0.48074281366641713
1106	12	0.5635212062900266
1106	13	0.39613971010633264
1106	14	-0.19699285922530407
1106	15	0.2436124920345435
1106	16	0.1246296418402224
1106	17	0.14461722824118795
1106	18	0.12060687678687998
1106	19	-1.2335808485049071
1106	20	0.008818975784997463
1106	21	-0.23215053924212015
1106	22	0.3446280927988994
1106	23	0.2503413812966926
1106	24	0.39097027315801114
1106	25	0.7263351966092131
1107	1	-0.010901601736154731
1107	2	-0.10969659583317082
1107	3	-0.24397462168862916
1107	4	-0.0408351749941005
1107	5	-0.04254821514533802
1107	6	-0.029404344700544982
1107	7	-0.16421774279853452
1107	8	-0.341015458124204
1107	9	0.5205433814590705
1107	10	0.3614710193027548
1107	11	0.4197337768694709
1107	12	0.24208148632553161
1107	13	0.09754999498923667
1107	14	0.20125602501252615
1107	15	0.09774708619689676
1107	16	-0.09172353650914289
1107	17	-0.2515711659070021
1107	18	-0.13878109904074165
1107	19	0.08773734259553123
1107	20	-0.09065351674966138
1107	21	-0.12221195343242641
1107	22	-0.04323514442751275
1107	23	0.1002032131199968
1107	24	0.3343904219904701
1107	25	-0.054947054951064445
1108	1	0.0580688363663846
1108	2	0.12567009021831055
1108	3	0.17215740583942107
1108	4	0.019826547103797054
1108	5	0.22507334095120513
1108	6	0.22275759240616821
1108	7	-0.19102318558352493
1108	8	0.22825898481612228
1108	9	0.4440738402356672
1108	10	0.2892660216189
1108	11	0.28624936077151375
1108	12	0.32258378361614604
1108	13	-0.02566234413036028
1108	14	0.07217150595942456
1108	15	-0.13378481450535418
1108	16	-0.7026500152526722
1108	17	-0.6047563755138015
1108	18	-0.7377629985148602
1108	19	-0.5629191521759296
1108	20	0.03675201342267087
1108	21	-0.21975867331104126
1108	22	-0.06626089463188123
1108	23	-0.42660352698293735
1108	24	-0.11557998186287222
1108	25	-0.3704311673036429
1109	1	0.15511340255427442
1109	2	0.18787668039549743
1109	3	0.1282127896291776
1109	4	0.18601735130635433
1109	5	0.20428688839335507
1109	6	0.3845074232003114
1109	7	0.5458695646900509
1109	8	0.38164419508789393
1109	9	1.2105795237952037
1109	10	0.44432383022832056
1109	11	0.6479448828064278
1109	12	0.6287337898099992
1109	13	0.6883804350550236
1109	14	0.3302873649315359
1109	15	-0.0366266585684869
1109	16	0.3984374029895008
1109	17	0.36245137842251296
1109	18	0.23708690227628182
1109	19	0.5259425370879498
1109	20	0.8897126980816665
1109	21	0.8053577776136862
1109	22	0.5342664618517574
1109	23	0.8160112747472255
1109	24	0.6762248392289862
1109	25	0.7082395925946301
1110	1	-0.02730000252972431
1110	2	0.0683580493052034
1110	3	-0.07289170558329161
1110	4	0.0932503416648629
1110	5	0.09311321690179142
1110	6	0.19776842455393506
1110	7	0.21147076717807067
1110	8	0.07520448973067317
1110	9	-3.4793222342622165
1110	10	-1.4067725681207506
1110	11	-0.4571127502520298
1110	12	-0.01239910670793165
1110	13	-0.2966227206455869
1110	14	-0.1354741139294262
1110	15	0.07283857474574518
1110	16	-0.3530868834484622
1110	17	-0.24351414139611194
1110	18	-0.12583714499279353
1110	19	-0.31493811099329605
1110	20	0.21895462405557709
1110	21	-0.1111708271514189
1110	22	0.3427366114153614
1110	23	0.5498102815319825
1110	24	0.5939858281259021
1110	25	0.6228621126707026
1111	1	0.11208601795385675
1111	2	0.06563708656681655
1111	3	0.11068321736445341
1111	4	-0.11360580064424397
1111	5	0.03548768331806684
1111	6	0.06220899753109421
1111	7	0.1563305144768492
1111	8	0.1814880822511995
1111	9	0.8819374506908488
1111	10	0.4017045625689235
1111	11	0.42266247839079796
1111	12	0.6557834656558228
1111	13	0.2372856764161213
1111	14	0.08847367656888849
1111	15	0.17996345721905982
1111	16	0.19990495630295868
1111	17	0.22204664315421496
1111	18	0.10211924820750795
1111	19	0.33279343543162554
1111	20	0.5286630824574132
1111	21	0.4540084670348392
1111	22	0.5133996881759941
1111	23	0.6454665706551665
1111	24	0.6852877221281446
1111	25	0.774300337628091
1112	1	-0.17203748906121774
1112	2	-0.28758185834896466
1112	3	-0.19974596872501138
1112	4	-0.09824929861197518
1112	5	-0.10736303630278599
1112	6	-0.20580229229157965
1112	7	-0.0073368434156861895
1112	8	-0.5092211860591114
1112	9	0.11021100382047422
1112	10	-0.02208301090112741
1112	11	0.07051711318827358
1112	12	-0.552892450984956
1112	13	-0.35662614899312217
1112	14	-1.1822239391343672
1112	15	-0.392987977890364
1112	16	-0.5495105934271507
1112	17	-0.578376382623432
1112	18	-0.71582966342649
1112	19	-0.3293482325560859
1112	20	-0.3150857426256195
1112	21	-0.4437076185558979
1112	22	-0.21088122409826582
1112	23	-0.024276055405337615
1112	24	-0.06035272034890227
1112	25	-0.25772938705402026
1113	1	-2.300874078944066
1113	2	-2.3377595209378583
1113	3	-2.3959739752660916
1113	4	-2.3535007851728302
1113	5	-2.3265569461210838
1113	6	-2.2181800147232025
1113	7	-2.5138483279817905
1113	8	-2.577796900386854
1113	9	-11.464348983493437
1113	10	-4.902120939998252
1113	11	-3.4709033480025653
1113	12	-13.357653750964221
1113	13	-5.343142161228592
1113	14	-4.414369602366175
1113	15	-3.73257340553986
1113	16	-3.4520948450306683
1113	17	-3.4986786386369424
1113	18	-3.8029286400010065
1113	19	-4.792063305820378
1113	20	-15.365683524139378
1113	21	-18.005209118570605
1113	22	-12.739084442546478
1113	23	-18.84237873232707
1113	24	-19.973334825650582
1113	25	-16.22239042534691
1114	1	-0.09868480326472451
1114	2	-0.12140697574376702
1114	3	-0.13947169760922407
1114	4	-0.5964150075472645
1114	5	-0.23776202362033416
1114	6	-0.14487463194059558
1114	7	-0.247225299526729
1114	8	-0.041510714734801724
1114	9	0.6394913632554236
1114	10	-0.012300243333541516
1114	11	-0.12705615171162776
1114	12	-0.09125222786174447
1114	13	-0.11013399701634086
1114	14	-0.13869321008627367
1114	15	-0.08632076791377585
1114	16	-0.05616903367295123
1114	17	-0.112714307811423
1114	18	-0.16811400113022046
1114	19	-0.35250333366045083
1114	20	-0.8272459930678693
1114	21	-0.9312243054047542
1114	22	-1.0274221765264642
1114	23	-0.5070989874540999
1114	24	-0.19996904905612922
1114	25	-0.4538778807420272
1115	1	-0.610263118853112
1115	2	-0.5359748031689975
1115	3	-0.5618791409121897
1115	4	-0.57335599498996
1115	5	-0.8165974661853248
1115	6	-0.8391557688939102
1115	7	-1.132860883148286
1115	8	-0.684988833451186
1115	9	-0.35082687811478763
1115	10	-0.7290815379245785
1115	11	-0.6075506437328215
1115	12	-0.787446970001465
1115	13	-1.0656075290588376
1115	14	-1.0646275347078813
1115	15	-1.085895029293468
1115	16	-0.9344446871688916
1115	17	-0.8390328014019914
1115	18	-0.9503086451842435
1115	19	-0.8462912396943523
1115	20	-1.2473930281942245
1115	21	-0.7315461198966773
1115	22	-1.3598595826736357
1115	23	-1.0308326894650897
1115	24	-0.5580072884646299
1115	25	-0.40020398765076237
1116	1	-1.3599111702812756
1116	2	-1.2359193929869043
1116	3	-1.2633748049480467
1116	4	-1.1224379703629523
1116	5	-2.187403343890825
1116	6	-2.238867917693728
1116	7	-3.2483153933844946
1116	8	-2.357511898526454
1116	9	-1.6015505562238914
1116	10	-2.4952630133145917
1116	11	-2.3412558239978765
1116	12	-3.439993020910741
1116	13	-3.046414240264962
1116	14	-2.4970379128471585
1116	15	-3.82845609495336
1116	16	-4.158551814303561
1116	17	-4.745839449215108
1116	18	-3.911424549902211
1116	19	-3.2586830460310736
1116	20	-3.8362586925470445
1116	21	-3.1423912543074586
1116	22	-2.965035328040372
1116	23	-3.1757203958005142
1116	24	-3.6896880013776645
1116	25	-3.2144878990930827
1201	1	0.7955298988226289
1201	2	0.8720545943945055
1201	3	0.43694663988783944
1201	4	0.9326826067723104
1201	5	1.0286478033941933
1201	6	1.1549264850983718
1201	7	1.2414304407784869
1201	8	1.300461825995022
1201	9	1.951357233170107
1201	10	1.770199886306429
1201	11	1.7505139987622442
1201	12	1.8274891358286922
1201	13	1.6376747825072542
1201	14	1.7453790710585864
1201	15	1.6981770373578442
1201	16	1.5509736709522954
1201	17	1.5123120014759834
1201	18	1.507670778222554
1201	19	1.560156847957515
1201	20	1.7379796872369226
1201	21	1.8121727497852689
1201	22	1.8127512664975285
1201	23	2.0781360879353556
1201	24	2.1689878220146355
1201	25	2.271674004223094
1202	1	0.8471654434098497
1202	2	0.9096704524172807
1202	3	0.8661380302012134
1202	4	0.9517372378010052
1202	5	1.0996697654320116
1202	6	1.049988767737645
1202	7	1.133930784964143
1202	8	1.2132781026843773
1202	9	1.8029743071730167
1202	10	1.4692858489188865
1202	11	1.5216283992335846
1202	12	1.631799280882639
1202	13	1.4694864304339745
1202	14	1.6407654440429624
1202	15	1.5638001160108885
1202	16	1.484845558637467
1202	17	1.3983912522543562
1202	18	1.461843206257825
1202	19	1.5868194784594607
1202	20	1.71430655501063
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2211	7	0.4333920966854876
2211	8	0.34996033558634565
2211	9	0.8741132284979827
2211	10	0.22338235649350935
2211	11	0.5574169383885135
2211	12	0.6173943061642717
2211	13	0.5234281238257097
2211	14	0.4943803374788658
2211	15	0.5209687189728882
2211	16	0.536375788655036
2211	17	0.37336741867335377
2211	18	0.36528300653380086
2211	19	0.09114183061107184
2211	20	0.6277435439881174
2211	21	0.7722342421933917
2211	22	0.6180572210456035
2211	23	1.040620206272095
2211	24	1.1321679494982035
2211	25	1.2613075807167107
2212	1	0.10087438314671715
2212	2	0.06803588577748343
2212	3	0.1889961364644428
2212	4	0.26024862177939667
2212	5	0.039171182711982055
2212	6	0.15113417111337668
2212	7	0.126817752496045
2212	8	0.08942835203903599
2212	9	0.5957050917568836
2212	10	0.28599548673918207
2212	11	0.4607623249535848
2212	12	0.24417882520854306
2212	13	0.033637734903564365
2212	14	0.17650271486637337
2212	15	0.30720061063654214
2212	16	0.4675860768650253
2212	17	0.20228442410600422
2212	18	0.2323248297885243
2212	19	0.4147805491340222
2212	20	0.6339696809283453
2212	21	0.7716217345014371
2212	22	0.35350495292287576
2212	23	0.7756217146753741
2212	24	0.8514634711898168
2212	25	0.9232549143515243
2213	1	-0.2917558989554614
2213	2	-0.2796426200805442
2213	3	-0.4054026050683923
2213	4	-0.46342462442651566
2213	5	-0.3085843709131524
2213	6	-0.13839797611576898
2213	7	-0.242457920075001
2213	8	-0.22832687953860642
2213	9	0.5207274504555031
2213	10	-0.06434577320041512
2213	11	0.1499184760339034
2213	12	0.16077130548351448
2213	13	-0.2587668398761238
2213	14	-0.15826454187300243
2213	15	-0.049254869267883805
2213	16	-0.25177207564524906
2213	17	-0.33198736676503415
2213	18	-0.4844280879959513
2213	19	-0.41114342070802223
2213	20	0.017767982055531206
2213	21	-0.19847969675220156
2213	22	-0.009207003619138732
2213	23	-0.4665473733493428
2213	24	-0.3160446936275392
2213	25	-0.07714140200386765
2214	1	-0.9096698100846936
2214	2	-0.7105937188337705
2214	3	-0.7219228590710927
2214	4	-0.5469156415831051
2214	5	-0.8663612364814846
2214	6	-0.7488106983053555
2214	7	-0.6344090930166892
2214	8	-0.576952181050693
2214	9	-10.420973411681494
2214	10	-3.758897432737637
2214	11	-1.7100267388159978
2214	12	-1.0369241425342075
2214	13	-0.8058097361632086
2214	14	-0.8399137342418612
2214	15	-0.9611326554552093
2214	16	-1.0941270041463511
2214	17	-0.9230075602395544
2214	18	-1.2297861936455803
2214	19	-1.5447946403613813
2214	20	-1.2459707437844898
2214	21	-1.7415263231147584
2214	22	-1.598179831780488
2214	23	-1.8106222586572933
2214	24	-2.11118935407967
2214	25	-9.917100618544744
2215	1	-0.44925472420482393
2215	2	-0.08976110355956735
2215	3	-0.08109706228913183
2215	4	-0.3226206589072973
2215	5	-0.1099501207691631
2215	6	-0.06540780934665946
2215	7	-0.23681773003116646
2215	8	-0.08955944761183332
2215	9	0.5766554936695201
2215	10	0.4457666687621682
2215	11	0.5019944344984566
2215	12	0.554390093465571
2215	13	0.3672246204954632
2215	14	0.18283148058593213
2215	15	-0.18830096155100762
2215	16	-0.021110363973903645
2215	17	-0.029192151695006885
2215	18	0.12471598577206322
2215	19	0.15040056081775077
2215	20	0.3715986726571572
2215	21	0.23850718483525668
2215	22	0.13555768380295774
2215	23	0.44907993288533277
2215	24	0.6057414919250164
2215	25	0.754222251474495
2216	1	-1.054464402059505
2216	2	-1.1532684114916059
2216	3	-1.1331372873829757
2216	4	-0.9777962457754938
2216	5	-1.8126746502113882
2216	6	-2.3791418481939886
2216	7	-1.8500106264936989
2216	8	-2.8105500865271154
2216	9	-1.4645408968984164
2216	10	-1.9747523466427168
2216	11	-1.569414314033157
2216	12	-2.7380063931574696
2216	13	-2.492196864418613
2216	14	-2.5994696092914253
2216	15	-2.268012120213207
2216	16	-3.1193307463731195
2216	17	-3.5499347323334938
2216	18	-3.100436074153407
2216	19	-4.44121590688183
2216	20	-3.3563511183700143
2216	21	-3.510363499211799
2216	22	-3.256936901115637
2216	23	-2.9820647246421395
2216	24	-3.1218754863195635
2216	25	-3.2193461478080145
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104903.0
3	6.0
4	1459.0
5	14395.0
6	19818.0
7	16174.0
8	12920.0
9	10460.0
10	8964.0
11	8103.0
12	7224.0
13	6879.0
14	6700.0
15	6760.0
16	7214.0
17	7666.0
18	8651.0
19	10707.0
20	14398.0
21	19287.0
22	23861.0
23	28457.0
24	32730.0
25	37601.0
26	41691.0
27	45558.0
28	51325.0
29	60211.0
30	72777.0
31	90012.0
32	111851.0
33	137563.0
34	171026.0
35	213660.0
36	277732.0
37	407464.0
38	951315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46746694999231	19.849895180674253	13.72655596879348	23.956081900539967
2	15.805079350912033	21.247944200380907	39.40014621936317	23.54683022934389
3	18.71677828212279	26.494815287330635	29.546709665606457	25.241696764940112
4	11.97893286079937	17.596738203058347	37.23882654065761	33.185502395484676
5	12.654398492131252	38.95117513382105	34.20054825025711	14.193878123790588
6	30.429959847660683	38.365355111095795	17.595125929353543	13.609559111889983
7	27.456388722688697	32.940393408166635	21.50898908745755	18.094228781687118
8	26.1206260177252	36.03136981503783	19.494010253319825	18.353993913917147
9	26.33639179467518	15.856062804374456	19.95531136300671	37.85223403794365
10	14.896873831580889	31.242743286401254	32.08343058191252	21.77695230010534
11	34.021822660599824	24.04960405845419	22.546559200179367	19.382014080766627
12	22.82843543915655	27.666790492292183	29.82880560339396	19.675968465157307
13	28.34594948638453	22.53033389599582	26.510985044378582	22.612731573241067
14	21.65362237514398	21.949391430474748	28.87740334918338	27.5195828451979
15	23.52944844143587	29.772368384782293	24.52728520929402	22.170897964487814
16	22.0616341435881	29.423399027064207	26.445128626522347	22.069838202825345
17	21.544401949864564	28.834800369915065	27.50744457345236	22.113353106768017
18	20.685030007722226	27.408321953470388	30.527077181889755	21.379570856917628
19	22.249550204172188	26.81239329451403	30.18381076346155	20.75424573785223
20	22.152827849846915	26.397498914505	30.48690235008436	20.96277088556372
21	22.994010089513917	25.67768820697812	30.09631376996801	21.231987933539955
22	22.22785225538618	26.249479021380633	30.105245885499563	21.41742283773362
23	21.824557787923947	26.14929524680883	30.411024807062926	21.6151221582043
24	21.730621181483283	26.41000852551847	30.324068149017396	21.535302143980847
25	21.768876423576653	26.19453311087613	30.427757088031232	21.60883337751599
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1091.0
1	1091.0
2	1972.5
3	2854.0
4	2854.0
5	2854.0
6	7808.5
7	12763.0
8	12763.0
9	12763.0
10	14191.0
11	15619.0
12	15619.0
13	15619.0
14	18147.5
15	20676.0
16	20676.0
17	20676.0
18	34232.0
19	47788.0
20	47788.0
21	47788.0
22	70745.5
23	93703.0
24	93703.0
25	93703.0
26	133526.0
27	173349.0
28	173349.0
29	173349.0
30	199303.0
31	225257.0
32	225257.0
33	225257.0
34	250751.5
35	276246.0
36	276246.0
37	276246.0
38	294751.0
39	313256.0
40	313256.0
41	313256.0
42	331552.0
43	349848.0
44	349848.0
45	349848.0
46	367368.0
47	384888.0
48	384888.0
49	384888.0
50	373643.0
51	362398.0
52	362398.0
53	362398.0
54	327289.5
55	292181.0
56	292181.0
57	292181.0
58	250879.0
59	209577.0
60	209577.0
61	209577.0
62	171834.5
63	134092.0
64	134092.0
65	134092.0
66	102194.0
67	70296.0
68	70296.0
69	70296.0
70	52678.0
71	35060.0
72	35060.0
73	35060.0
74	24784.0
75	14508.0
76	14508.0
77	14508.0
78	10998.0
79	7488.0
80	7488.0
81	7488.0
82	5151.5
83	2815.0
84	2815.0
85	2815.0
86	1958.5
87	1102.0
88	1102.0
89	1102.0
90	862.5
91	623.0
92	623.0
93	623.0
94	330.5
95	38.0
96	38.0
97	38.0
98	22.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027924326715278835
2	0.001509423065690748
3	1.6406772453160306E-4
4	0.004003252478571114
5	0.036193340031671635
6	0.012370706429682871
7	0.021689753183077923
8	0.02949937687078223
9	0.036423034846015875
10	0.03954032161211633
11	0.040655982138931235
12	0.007415861148828458
13	0.0034782357600699845
14	0.006956471520139969
15	0.00754711532845374
16	0.008203386226580154
17	0.011025351088523724
18	0.01552080674068965
19	0.019163110225291238
20	0.01722711107581832
21	0.013059790872715603
22	0.012206638705151268
23	0.009351860298301373
24	0.0065627089812641225
25	0.10963005353201716
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3047522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.93601052825064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12333449052427	34.04930273545767
2	13.569205248899808	12.466303103469109
3	5.0504566192620395	6.959934853046826
4	2.323592171858159	4.269462178793487
5	1.2844428973421198	2.950109122762219
6	0.793047605393803	2.185766589046422
7	0.5017600501104611	1.61341984611708
8	0.3606535692158285	1.3253588922039574
9	0.27237387560109655	1.1260592295509162
>10	1.4595245651830815	12.596152896585638
>50	0.1490169860087223	4.777770293360518
>100	0.10432304063864631	8.926056813666012
>500	0.005824167625436898	1.863822180491314
>1k	0.0020132925124549837	1.5708174034351368
>5k	1.4380660803249885E-4	0.5555563464775433
>10k+	2.876132160649977E-4	2.7641075155362476
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	30056	0.9862439057043723	No Hit
TATCAACGCAGAGTACTTTTTTTTT	23117	0.7585507175994136	No Hit
GGTATCAACGCAGAGTACTTTTTTT	20279	0.6654258771552757	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	10234	0.3358138185712851	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	9537	0.31294277777157964	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	7283	0.23898104755273303	No Hit
GTGGTATCAACGCAGAGTACATGGG	4524	0.14844847715619444	No Hit
GTGGTATCAACGCAGAGTACTTTTT	3711	0.12177106514735579	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	3485	0.11435520399852733	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.562708981264122E-5	0.0
5	0.0	0.0	0.0	6.562708981264122E-5	0.0
6	0.0	0.0	0.0	6.562708981264122E-5	0.0
7	0.0	0.0	0.0	6.562708981264122E-5	0.0
8	0.0	0.0	0.0	6.562708981264122E-5	0.0
9	0.0	0.0	0.0	6.562708981264122E-5	0.0
10	0.0	0.0	0.0	6.562708981264122E-5	0.0
11	0.0	0.0	0.0	6.562708981264122E-5	0.0
12	0.0	0.0	0.0	6.562708981264122E-5	0.0
13	0.0	0.0	0.0	6.562708981264122E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	165	0.0	13.819901	2
GGTATCA	5265	0.0	13.735208	1
TAACGCA	385	0.0	13.574009	4
CGTATCA	70	7.25869E-6	13.573117	2
TATAACG	355	0.0	13.114307	2
ATAACGC	400	0.0	13.064769	3
ATCACGC	185	0.0	12.840067	3
TGGTATC	1450	0.0	12.580875	2
GCGTATC	70	1.0886272E-4	12.217812	1
GTGGTAT	1595	0.0	11.915664	1
CAACCGT	235	0.0	11.723901	6
GCCCCCG	230	0.0	11.561912	18
TAAACGC	190	0.0	11.502187	4
TATGACG	75	2.0714008E-4	11.402167	4
AACCGTC	75	2.0742453E-4	11.400483	7
TGCACGG	255	0.0	11.172542	12
TTGACCG	205	0.0	11.1187725	18
CGAAATC	120	1.2809323E-7	11.08071	13
ATTCGAT	60	0.0058889394	11.080528	16
TTGCGCG	225	0.0	10.974639	18
>>END_MODULE