FastQCFastQC Report
Thu 26 May 2016
SRR1364299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4495078
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT148080.3294269865839925No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN123760.27532336480034386No Hit
TATCAACGCAGAGTACTTTTTTTTT89280.19861724312681558No Hit
GGTATCAACGCAGAGTACTTTTTTT65890.14658255095907124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.013.4244311
GTGGTAT12250.011.5474831
GTTATCA7100.011.3657411
TAACGCA1803.6379788E-1211.0871684
TGGTATC11900.011.0092972
CGCATAT2350.010.913798
GTATCAA86550.010.7826031
TATAACG2050.010.651272
GGACCGA4400.010.5785936
CGCCCGA1002.3769908E-510.45957716
AGGACCG5900.010.3084195
ATAACGC1951.4551915E-1110.2237963
ACCGAGT3850.010.115868
TGCCGTA1451.5983096E-79.83648215
TGGACCG1651.0184522E-89.7910455
TTGCGCG2500.09.50849318
CGCGCCA2600.09.49882710
GTATTAG5050.09.3997391
GTATAAC4650.09.3916531
TCAACTC12650.09.39038855
Produced by FastQC (version 0.11.5)