##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364299_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4495078
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.903528036665882	30.0	26.0	31.0	2.0	34.0
2	25.82560213638117	30.0	25.0	31.0	2.0	34.0
3	25.217331712597645	30.0	23.0	31.0	2.0	34.0
4	28.983624088391792	35.0	28.0	37.0	2.0	37.0
5	29.511423828463045	35.0	29.0	37.0	2.0	37.0
6	29.731714332876983	35.0	30.0	37.0	2.0	37.0
7	29.35463789504876	35.0	29.0	37.0	2.0	37.0
8	28.907175359359726	35.0	28.0	37.0	2.0	37.0
9	29.973653627367533	35.0	28.0	39.0	2.0	39.0
10	29.16318604482503	35.0	27.0	39.0	2.0	39.0
11	29.861433105276483	35.0	28.0	39.0	2.0	39.0
12	29.329955787196575	35.0	27.0	39.0	2.0	39.0
13	29.01580817952436	35.0	26.0	39.0	2.0	39.0
14	29.212839910675633	36.0	25.0	40.0	2.0	41.0
15	29.947650074147766	36.0	27.0	40.0	2.0	41.0
16	30.261481113342192	36.0	27.0	40.0	2.0	41.0
17	30.409960850512494	36.0	27.0	40.0	2.0	41.0
18	30.21562384456955	36.0	27.0	40.0	2.0	41.0
19	30.187079067371023	37.0	27.0	40.0	2.0	41.0
20	30.060136442571185	37.0	27.0	40.0	2.0	41.0
21	29.96428827263954	37.0	27.0	40.0	2.0	41.0
22	30.045198993209908	37.0	27.0	40.0	2.0	41.0
23	30.04941916469525	37.0	27.0	40.0	2.0	41.0
24	29.91164402486453	37.0	27.0	40.0	2.0	41.0
25	29.69659280662093	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9791871368130884
1101	2	1.1350077159102128
1101	3	1.83669830221438
1101	4	1.3291442401468636
1101	5	0.7847030992072526
1101	6	0.707514875490407
1101	7	1.004766564104436
1101	8	1.2251849624025404
1101	9	1.2332120623387155
1101	10	1.51790538486906
1101	11	0.9573954487132426
1101	12	1.2716875646553198
1101	13	1.1217638444749518
1101	14	1.7247650203303948
1101	15	0.9335359553391598
1101	16	0.4449254402181708
1101	17	-0.09675694755425823
1101	18	-5.973136385956554
1101	19	-7.503574865115574
1101	20	-8.562460950613112
1101	21	-10.171925760189929
1101	22	-10.34755073058244
1101	23	-11.728803847136604
1101	24	-11.53424257992545
1101	25	-11.819876997551258
1102	1	2.17904072501711
1102	2	2.422706270963257
1102	3	3.187525633480842
1102	4	2.609290978648364
1102	5	1.973362397930174
1102	6	2.0509315893963063
1102	7	2.3230460226873078
1102	8	2.554748370943301
1102	9	2.7710221423604615
1102	10	-0.8675617622749776
1102	11	1.307725710372246
1102	12	2.327844463331548
1102	13	2.796800761740357
1102	14	3.435918003587748
1102	15	3.080863003996164
1102	16	2.718049827014383
1102	17	2.4853282834930823
1102	18	2.6591725144838882
1102	19	2.2440607550738356
1102	20	2.766185498304324
1102	21	2.838904289339915
1102	22	2.7041181702544463
1102	23	2.695684438864351
1102	24	2.891238780744324
1102	25	2.902106273841092
1103	1	-0.26074370495394206
1103	2	0.17409585568826458
1103	3	-2.8209934880956453
1103	4	-0.19673357044982964
1103	5	-2.7870660979504756
1103	6	-7.061354093079757
1103	7	-7.503245449145247
1103	8	-2.1666325854138577
1103	9	-7.803002991240074
1103	10	-4.616142952433073
1103	11	-8.365437978115843
1103	12	-1.9823189189886499
1103	13	-0.038555367184159195
1103	14	0.3779854718576203
1103	15	0.2684026666548185
1103	16	0.0804057889382328
1103	17	-0.1680472175089207
1103	18	0.37772072201005
1103	19	-5.485503234015454
1103	20	-1.1880243102015768
1103	21	-5.607148232370172
1103	22	-2.2218998379842496
1103	23	-1.3947304186284164
1103	24	-6.954083808879368
1103	25	-3.584593514624725
1104	1	1.7218741429976987
1104	2	1.776287947288349
1104	3	2.679251989505577
1104	4	2.095250113004699
1104	5	1.719714227183438
1104	6	1.600932318028363
1104	7	1.740765832537729
1104	8	1.998833812676935
1104	9	2.244974553871696
1104	10	2.5374198860922306
1104	11	1.975260150830504
1104	12	2.3898653360312494
1104	13	2.6411608887634124
1104	14	3.117897043812011
1104	15	2.09448529301266
1104	16	1.311731255766496
1104	17	1.1590004865040058
1104	18	1.1352543126974233
1104	19	1.6239662164139688
1104	20	-8.894364832989378
1104	21	-3.1811407922947623
1104	22	-0.7027249980824024
1104	23	0.4384603039080197
1104	24	1.0765228060172412
1104	25	1.5720081028154986
1105	1	1.2218370984330882
1105	2	1.111955125273834
1105	3	1.9937417280684748
1105	4	1.374246912821544
1105	5	1.1701894266261448
1105	6	1.1918986950243315
1105	7	1.4037316952854297
1105	8	1.4700512057192832
1105	9	1.4667697438556146
1105	10	2.0894560596831333
1105	11	1.5985253462680227
1105	12	1.8097737080249807
1105	13	1.9056890049761073
1105	14	2.656090249497712
1105	15	1.936710786577713
1105	16	1.3850212198896301
1105	17	0.5846268668914654
1105	18	1.2140142646232945
1105	19	1.1790960190480178
1105	20	1.1150916542759113
1105	21	1.144295299916763
1105	22	1.11230908050673
1105	23	1.1094194677821605
1105	24	1.1048407057839924
1105	25	1.0618464895058004
1106	1	1.0812701839349472
1106	2	1.3394652508594866
1106	3	1.9609378681497383
1106	4	1.6086757052338356
1106	5	0.7676541225471816
1106	6	0.5768418911528137
1106	7	1.1244491800737748
1106	8	1.0608210669635731
1106	9	1.3602107005559354
1106	10	-2.0271085535663573
1106	11	0.07526863065479361
1106	12	0.658610189166069
1106	13	1.5655766542496927
1106	14	0.5274219094038131
1106	15	1.299733505531389
1106	16	0.9097318236758554
1106	17	-5.75552643036923
1106	18	-1.6282228601530448
1106	19	-0.18617227195583297
1106	20	0.5670954853145602
1106	21	0.8429279871420228
1106	22	0.985351794191164
1106	23	0.5099451480136139
1106	24	0.9196766616798833
1106	25	1.043078017772416
1107	1	0.7387244395490313
1107	2	1.0740410817918296
1107	3	1.590695877489793
1107	4	1.1871351801277505
1107	5	0.6093943916838747
1107	6	0.7677552005776391
1107	7	0.718356836533836
1107	8	0.8708403645599567
1107	9	1.043194506680635
1107	10	-2.234769831495388
1107	11	-0.190422864756016
1107	12	0.3122924462579384
1107	13	1.1208814339432784
1107	14	1.5715558442816082
1107	15	1.3554917180363901
1107	16	-5.619632042593047
1107	17	-2.037593412407727
1107	18	-0.29912676589926335
1107	19	0.6317913590597399
1107	20	1.0543448870383578
1107	21	1.0980387304798427
1107	22	0.7568947704930942
1107	23	0.3767291083715527
1107	24	0.6758785713689157
1107	25	0.9492632992864962
1108	1	-0.4139547736202296
1108	2	-3.203966656101965
1108	3	-0.1411423986785536
1108	4	-0.7125619518872952
1108	5	-0.9177932013271999
1108	6	-0.6015116105292933
1108	7	-0.22256727521579833
1108	8	0.02183716086042864
1108	9	-0.46741477675224274
1108	10	-3.5419735740139053
1108	11	-1.6758186845863179
1108	12	-6.03591140153911
1108	13	-2.0564264056674624
1108	14	-6.264133939574187
1108	15	-2.8683846140469385
1108	16	-7.798034010156275
1108	17	-3.4949872995689084
1108	18	-2.117563186686425
1108	19	-0.8130210372601248
1108	20	-0.8056731028822028
1108	21	-0.3120948226361584
1108	22	-0.5772661603762508
1108	23	-0.4382039377757465
1108	24	-0.2630054746028172
1108	25	-0.46805300340042777
1109	1	-8.525843075733562
1109	2	-6.317545872365756
1109	3	-5.608660384964889
1109	4	-5.575175098994951
1109	5	-2.86118173637896
1109	6	-1.9107248726991557
1109	7	-1.5065450194337657
1109	8	-0.9492273521419108
1109	9	-6.005057549251124
1109	10	-1.976856964096669
1109	11	-1.4999549363724398
1109	12	-6.329230367730208
1109	13	-8.281056518197051
1109	14	-8.414371802543855
1109	15	-4.32663071271903
1109	16	-2.6379240913564246
1109	17	-2.2436514992768757
1109	18	-1.5852933970814185
1109	19	-1.278088792640375
1109	20	-1.0058611733077747
1109	21	-0.9621033428674082
1109	22	-1.2377051633372282
1109	23	-1.5568716862772796
1109	24	-1.1678767800273135
1109	25	-1.0998290885964863
1110	1	-2.6199307864080907
1110	2	-2.467643842958722
1110	3	-1.7162182215929782
1110	4	-2.544370067648135
1110	5	-5.676909527634773
1110	6	-4.420090597111464
1110	7	-9.191898585676611
1110	8	-10.154800123339239
1110	9	-11.657047937939542
1110	10	-9.56743147573497
1110	11	-12.238757388393278
1110	12	-7.169054432177113
1110	13	-5.361431460528426
1110	14	-4.900048558482684
1110	15	-10.257613374755596
1110	16	-11.961053740240391
1110	17	-12.689519046810545
1110	18	-9.606699122188395
1110	19	-12.73139251582494
1110	20	-13.986870319495003
1110	21	-14.877876929240474
1110	22	-15.678514143710641
1110	23	-16.23618909363183
1110	24	-16.516109741638694
1110	25	-17.234172406246977
1111	1	-0.8242209860429774
1111	2	-0.034111005974722275
1111	3	-2.948442439530524
1111	4	-3.600086219456454
1111	5	-1.3908763444552612
1111	6	-0.7829784492239789
1111	7	-0.2789996801974901
1111	8	0.0805965352631084
1111	9	-4.537240022145152
1111	10	-4.447073324029834
1111	11	-1.5425328088737515
1111	12	-6.0190416930872495
1111	13	-1.5008553119965207
1111	14	-0.050684506067327106
1111	15	0.04078815230603183
1111	16	-0.03186656205514282
1111	17	-0.10302928152658097
1111	18	0.15963757263550704
1111	19	0.36837599703678947
1111	20	0.3420299576424277
1111	21	0.5083663923538886
1111	22	0.02500735480456484
1111	23	-0.033193902807958864
1111	24	0.18611578641128546
1111	25	0.38213293864973963
1112	1	-0.47302070342266944
1112	2	0.06525309056693374
1112	3	-2.80591634821846
1112	4	-0.7275851267241471
1112	5	-0.584546576161074
1112	6	-0.423994524961504
1112	7	-0.05870139376938255
1112	8	-3.7755368749340086
1112	9	-0.9477105745616896
1112	10	-3.438807906182351
1112	11	-1.0416865816096461
1112	12	0.1791206152228213
1112	13	0.6516202117311387
1112	14	1.4068433446368829
1112	15	0.6907596077788973
1112	16	0.4819264669957022
1112	17	0.16169499526002795
1112	18	0.20720243266177718
1112	19	0.41683093331528553
1112	20	0.4642129317865802
1112	21	0.42454342492134955
1112	22	0.2511834816769962
1112	23	0.07195094553352632
1112	24	0.29713837818605526
1112	25	0.2966404695000264
1113	1	-2.1225966326567587
1113	2	-1.719584908096511
1113	3	-4.299090377151117
1113	4	-2.668263430519797
1113	5	-2.813784509577996
1113	6	-2.504486693446239
1113	7	-2.265884962721792
1113	8	-9.15004973100773
1113	9	-10.322048156157617
1113	10	-4.695027989081414
1113	11	-9.720434958792168
1113	12	-8.86994281537379
1113	13	-4.153146509113192
1113	14	-2.500512779016944
1113	15	-7.985556291366173
1113	16	-4.399190350492052
1113	17	-3.565309523855749
1113	18	-3.0039217900672455
1113	19	-2.786136771413421
1113	20	-8.10491482327837
1113	21	-4.35576611093089
1113	22	-3.5478748713009267
1113	23	-3.515531997005894
1113	24	-8.997962910994978
1113	25	-6.378826606412165
1114	1	-4.84391276056585
1114	2	-4.620941648940693
1114	3	-1.4416561034170954
1114	4	-1.1299784504189176
1114	5	-1.2821209108600407
1114	6	-1.3653176893379495
1114	7	-1.127710100001
1114	8	-0.8360089539755791
1114	9	-1.2883742236701572
1114	10	-4.095183077455211
1114	11	-2.042396279247722
1114	12	-5.603808457541838
1114	13	-2.254946862378091
1114	14	-6.543626130325638
1114	15	-2.993302309677116
1114	16	-2.009609094170493
1114	17	-1.8452113060468136
1114	18	-1.4008459913487243
1114	19	-1.1657596682681373
1114	20	-0.9216443370897878
1114	21	-0.8712290721035423
1114	22	-0.8917692912840955
1114	23	-1.2967104557405875
1114	24	-1.156487546662941
1114	25	-1.0817955103097319
1115	1	0.292622840939778
1115	2	0.3885433194563781
1115	3	1.1273005940299612
1115	4	0.014015221820699253
1115	5	-0.30510655688767585
1115	6	-0.9846632718237167
1115	7	-3.146512357357807
1115	8	-0.8369979235781102
1115	9	-0.4828791989695418
1115	10	0.21122721664905342
1115	11	-0.6159663597583531
1115	12	0.1668356703833318
1115	13	0.36203340093178227
1115	14	0.8720977906981986
1115	15	-0.03617160870750169
1115	16	-0.2436431241052439
1115	17	-0.6832193189581943
1115	18	-0.3017780702604824
1115	19	-0.08415963498163848
1115	20	-0.050494116964859614
1115	21	-0.0038118797131652116
1115	22	-0.44223498291178487
1115	23	-0.5124085587250349
1115	24	-0.7225016832755031
1115	25	-0.428343514624725
1116	1	-0.0333715681439557
1116	2	0.15385711774804278
1116	3	1.096298662036503
1116	4	0.386533286379926
1116	5	-0.6642984326590025
1116	6	-1.6660109400253305
1116	7	-4.995265582796591
1116	8	-1.5643007373939177
1116	9	-1.741086171476006
1116	10	-0.8648574042906532
1116	11	-2.672393904865448
1116	12	-0.8888200524466079
1116	13	-6.876218738227799
1116	14	-2.2149285357489035
1116	15	-1.9829701738660361
1116	16	-1.352331068858451
1116	17	-0.9276228556833566
1116	18	-0.9656901806737217
1116	19	-0.533899807319564
1116	20	-0.4491352870167198
1116	21	-0.31976728574476
1116	22	-0.46658608231696164
1116	23	-0.6932015261941125
1116	24	-0.5182484073881461
1116	25	-0.5210131035716294
1201	1	2.320065678455311
1201	2	2.5467269366576097
1201	3	3.271788978192184
1201	4	2.8806169797689023
1201	5	2.3141970279984996
1201	6	2.2674448215160723
1201	7	2.524752190626849
1201	8	2.6417395996009496
1201	9	3.0479453750161802
1201	10	3.2736613234129273
1201	11	2.8255208550746786
1201	12	3.1862001319592004
1201	13	3.2205949789575463
1201	14	3.898286062666262
1201	15	3.398640596795076
1201	16	2.910030360489216
1201	17	2.722856838099254
1201	18	2.7741337769266856
1201	19	2.7853345623284476
1201	20	2.9072384154858213
1201	21	2.7437832214249838
1201	22	1.8040210925017774
1201	23	1.3395074025204288
1201	24	1.910266508323236
1201	25	1.933376445086033
1202	1	2.4589168718414918
1202	2	2.6196375555422406
1202	3	3.44656467266368
1202	4	3.072560701420606
1202	5	2.4039455342013873
1202	6	2.3837706912027343
1202	7	2.562747515145208
1202	8	2.936640098111962
1202	9	3.213235973979625
1202	10	-0.5605147996386997
1202	11	1.776145305436529
1202	12	2.8916232355314655
1202	13	3.4531996606931834
1202	14	4.068895467875141
1202	15	3.5625770493708657
1202	16	3.2430920527109706
1202	17	2.762825691690601
1202	18	3.000712889805083
1202	19	3.1381796446517463
1202	20	3.1434629790590094
1202	21	3.290827756611641
1202	22	3.1008571147570194
1202	23	2.833952957848421
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2211	17	-0.004865888814421737
2211	18	0.37339114392301553
2211	19	0.7483015760132616
2211	20	1.021525336363478
2211	21	0.949199100300472
2211	22	0.8304989636503883
2211	23	0.704494371519587
2211	24	1.034709818246764
2211	25	1.019646324120579
2212	1	0.9548489782236835
2212	2	1.0281183819824058
2212	3	1.771762218761399
2212	4	1.3085491282986261
2212	5	0.7441627859439954
2212	6	0.77670190315974
2212	7	0.9992150361946663
2212	8	1.3157942243281937
2212	9	1.1649620404891685
2212	10	1.998395394414569
2212	11	1.1791197588252231
2212	12	1.4534936287521099
2212	13	1.58890420006421
2212	14	-4.364236456575309
2212	15	-0.8777804595570622
2212	16	-0.23865764751514007
2212	17	0.12107725367035016
2212	18	0.5180932468352424
2212	19	1.0431513211875547
2212	20	1.2557535997813858
2212	21	1.2330404905277668
2212	22	1.1715602287059212
2212	23	1.0421461972127055
2212	24	1.0653852404184754
2212	25	1.1405941432870215
2213	1	0.8564826244516013
2213	2	1.0058162934101453
2213	3	1.6550014416573582
2213	4	1.1843436445966233
2213	5	0.5437460385401955
2213	6	0.36619896984040423
2213	7	0.4866159452198886
2213	8	-4.997344166726645
2213	9	-1.4845397859375602
2213	10	0.35273616419204856
2213	11	0.18873458967628665
2213	12	0.8804138213995962
2213	13	-7.948403401401244
2213	14	-2.188154342327742
2213	15	-0.7017161584074998
2213	16	-0.15644768375607754
2213	17	0.08115188174328836
2213	18	0.3072773350472886
2213	19	0.6586435576606569
2213	20	0.8458590080248776
2213	21	0.8267296568208558
2213	22	0.4847648931132653
2213	23	0.45690985820518293
2213	24	0.593338107428508
2213	25	0.746567732448316
2214	1	-1.0368250056288524
2214	2	-0.7479716523517794
2214	3	-6.890442886791121
2214	4	-2.6544002031444904
2214	5	-1.6406177297392155
2214	6	-1.2683511941351533
2214	7	-0.7752876197300189
2214	8	-6.314061895329154
2214	9	-1.9948959964038195
2214	10	-0.32580473279272226
2214	11	-0.7411549290149679
2214	12	-0.3489943617571427
2214	13	-6.502266893886155
2214	14	-1.8099646572360086
2214	15	-1.0681346515949457
2214	16	-0.9675733012914165
2214	17	-0.9050805270618483
2214	18	-0.8250695121703195
2214	19	-0.46133993623948655
2214	20	-0.2125931488118944
2214	21	-0.13979745441120883
2214	22	-0.34317636445976873
2214	23	-0.34751286755716393
2214	24	-0.20924047645195643
2214	25	-0.13171127292521945
2215	1	0.21249265089529956
2215	2	0.488319729892865
2215	3	-2.766803877229272
2215	4	-0.6864270359417546
2215	5	-0.20682411312424165
2215	6	-0.7092860558795593
2215	7	-0.007516129517249226
2215	8	0.32268306558956894
2215	9	0.5592079118436253
2215	10	1.0993382639154348
2215	11	0.25503589608876
2215	12	-0.04744679466167767
2215	13	0.7471687517585721
2215	14	0.8633378947651096
2215	15	0.5243543920586369
2215	16	0.2482517874698047
2215	17	0.16066515400355996
2215	18	0.30645297849561004
2215	19	0.28229993534333175
2215	20	0.5035842311591665
2215	21	0.6530435811393716
2215	22	0.4947408252560841
2215	23	0.37291673017236704
2215	24	0.15988767912846313
2215	25	0.2806383747536394
2216	1	0.004436028571198847
2216	2	-0.29625447361320667
2216	3	0.9722392566051958
2216	4	-0.5270264921807915
2216	5	-0.5985859612389888
2216	6	-0.883558211060695
2216	7	-1.4844733081474573
2216	8	-6.4441543961315695
2216	9	-2.339848486219818
2216	10	-1.028637386602135
2216	11	-1.8665061895146629
2216	12	-0.5076226543339999
2216	13	0.19679028926584508
2216	14	0.965083998074519
2216	15	0.500452861887041
2216	16	-0.304096185402674
2216	17	-0.37339059869616875
2216	18	-0.1093127267989864
2216	19	-0.07711405888382927
2216	20	0.04423536822795526
2216	21	-0.10204815750668672
2216	22	-0.2951593231387726
2216	23	-0.5154701114434772
2216	24	-0.5390826100850141
2216	25	-0.37047355200753884
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	454534.0
3	20.0
4	3223.0
5	19660.0
6	27205.0
7	25207.0
8	22427.0
9	19225.0
10	16917.0
11	15367.0
12	14364.0
13	13787.0
14	13922.0
15	14518.0
16	15063.0
17	16228.0
18	18898.0
19	23815.0
20	33210.0
21	41276.0
22	51488.0
23	60250.0
24	70213.0
25	78373.0
26	87770.0
27	98025.0
28	106651.0
29	123509.0
30	141093.0
31	157937.0
32	193616.0
33	241421.0
34	297168.0
35	364081.0
36	446526.0
37	513604.0
38	654487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.433299598349834	17.289930531529116	13.597021261968145	23.679748608152913
2	14.942570300488045	19.06890135341944	41.22904646341267	24.759481882679836
3	17.590057724545176	24.458222650158717	29.521739295442202	28.429980329853905
4	11.497208489905557	15.30157864092929	36.334258776379144	36.86695409278601
5	13.799590401197406	37.55335155012807	33.454371796311555	15.192686252362964
6	33.62691738530497	35.193336165639955	16.87934998563021	14.300396463424866
7	29.914194043925903	31.360010568423174	20.046620670818164	18.679174716832758
8	26.796103029303598	34.61356424112768	19.689480370434918	18.9008523591338
9	26.75243577024163	16.06122194864297	19.24313162107533	37.943210660040066
10	13.948143590953343	29.60720108264652	32.807647509023916	23.637007817376226
11	35.43097259843883	23.379815222385393	21.50905512065796	19.680157058517818
12	23.277093216821605	25.1835148994283	29.655284756034973	21.884107127715126
13	28.364671774740764	21.466205736575912	26.167964882678596	24.001157606004735
14	21.452111558763377	20.648903636248008	28.017686417540926	29.881298387447686
15	24.17660463612495	28.704132813383797	23.14456713799644	23.97469541249481
16	24.065143149135952	28.32198486449337	24.320369339386346	23.292502646984335
17	22.620756899796625	28.104292103569183	25.70462768040323	23.57032331623096
18	22.3830449969822	27.16803387966071	27.58075258632519	22.868168537031895
19	23.86262340476092	26.509523740459805	27.333765461505077	22.294087393274197
20	23.89700185736335	26.051158767114984	27.569850120816934	22.48198925470473
21	24.387674615013015	25.530848605813265	27.406008284904317	22.675468494269406
22	23.891143675541	25.523448553935367	27.22925434506648	23.356153425457155
23	23.386417140864314	25.803224209304293	27.173616917024727	23.63674173280667
24	23.46714958749273	25.839462444144058	27.395864618537324	23.297523349825887
25	23.365199536185994	25.53583034599302	27.60492096219318	23.494049155627806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14118.0
1	14118.0
2	9129.5
3	4141.0
4	4141.0
5	4141.0
6	7203.5
7	10266.0
8	10266.0
9	10266.0
10	13342.0
11	16418.0
12	16418.0
13	16418.0
14	22090.5
15	27763.0
16	27763.0
17	27763.0
18	41843.5
19	55924.0
20	55924.0
21	55924.0
22	78291.5
23	100659.0
24	100659.0
25	100659.0
26	135720.0
27	170781.0
28	170781.0
29	170781.0
30	212958.5
31	255136.0
32	255136.0
33	255136.0
34	293692.5
35	332249.0
36	332249.0
37	332249.0
38	374545.5
39	416842.0
40	416842.0
41	416842.0
42	456452.5
43	496063.0
44	496063.0
45	496063.0
46	529902.0
47	563741.0
48	563741.0
49	563741.0
50	567864.0
51	571987.0
52	571987.0
53	571987.0
54	540510.5
55	509034.0
56	509034.0
57	509034.0
58	454589.0
59	400144.0
60	400144.0
61	400144.0
62	332105.0
63	264066.0
64	264066.0
65	264066.0
66	209608.5
67	155151.0
68	155151.0
69	155151.0
70	116377.5
71	77604.0
72	77604.0
73	77604.0
74	54513.0
75	31422.0
76	31422.0
77	31422.0
78	22704.0
79	13986.0
80	13986.0
81	13986.0
82	9475.5
83	4965.0
84	4965.0
85	4965.0
86	3421.0
87	1877.0
88	1877.0
89	1877.0
90	1242.0
91	607.0
92	607.0
93	607.0
94	350.0
95	93.0
96	93.0
97	93.0
98	67.0
99	41.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33503311844644296
2	0.3373912532774737
3	0.34929316020767603
4	0.3510951311634637
5	0.35730192001117667
6	0.2993051511008263
7	0.30709144535422966
8	0.3059123779387143
9	0.3057566520536462
10	0.41467578538125477
11	0.30457758463813084
12	0.30473331052319896
13	0.29843753545544704
14	0.30417714664795586
15	0.29723622148492196
16	0.3017300255968862
17	0.29461112799377454
18	0.2966133179446497
19	0.2977256456951359
20	0.5029723622148492
21	0.2988379734456221
22	0.30035073918628336
23	0.2958124419642996
24	0.29943863043088464
25	0.2916300896224715
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4495078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.12471358250778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.94563468864787	55.75363921658898
2	6.752339427180163	8.659836635596054
3	2.4600610363972812	4.732521280633888
4	1.1465247204270372	2.9408227725059426
5	0.6516734297046771	2.089418601457147
6	0.43468410570856625	1.6724396266458208
7	0.27130915012415463	1.2178335080817515
8	0.20352684819058767	1.044088067725758
9	0.15324713077090238	0.8844235531222705
>10	0.8606775810823771	10.136882395991268
>50	0.0755039595646823	3.3570144212175905
>100	0.04161366243572585	4.812716412031753
>500	0.002298708474799056	0.9596447485024804
>1k	7.662357079162964E-4	0.7850376284245958
>5k	6.965779162867487E-5	0.346555586920499
>10k+	6.965779162867487E-5	0.6071255445541563
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	14808	0.3294269865839925	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	12376	0.27532336480034386	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8928	0.19861724312681558	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6589	0.14658255095907124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.673966502917191E-5
2	0.0	0.0	0.0	0.0	8.898622003889588E-5
3	0.0	0.0	0.0	0.0	8.898622003889588E-5
4	0.0	0.0	0.0	0.0	8.898622003889588E-5
5	0.0	0.0	0.0	0.0	8.898622003889588E-5
6	0.0	0.0	0.0	0.0	8.898622003889588E-5
7	4.449311001944794E-5	0.0	0.0	0.0	8.898622003889588E-5
8	4.449311001944794E-5	0.0	0.0	0.0	8.898622003889588E-5
9	4.449311001944794E-5	0.0	0.0	0.0	1.1123277504861984E-4
10	4.449311001944794E-5	0.0	0.0	0.0	1.1123277504861984E-4
11	4.449311001944794E-5	0.0	0.0	8.898622003889588E-5	1.1123277504861984E-4
12	4.449311001944794E-5	0.0	0.0	1.1123277504861984E-4	2.4471210510696367E-4
13	4.449311001944794E-5	0.0	0.0	1.1123277504861984E-4	2.892052151264116E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3635	0.0	13.424431	1
GTGGTAT	1225	0.0	11.547483	1
GTTATCA	710	0.0	11.365741	1
TAACGCA	180	3.6379788E-12	11.087168	4
TGGTATC	1190	0.0	11.009297	2
CGCATAT	235	0.0	10.91379	8
GTATCAA	8655	0.0	10.782603	1
TATAACG	205	0.0	10.65127	2
GGACCGA	440	0.0	10.578593	6
CGCCCGA	100	2.3769908E-5	10.459577	16
AGGACCG	590	0.0	10.308419	5
ATAACGC	195	1.4551915E-11	10.223796	3
ACCGAGT	385	0.0	10.11586	8
TGCCGTA	145	1.5983096E-7	9.836482	15
TGGACCG	165	1.0184522E-8	9.791045	5
TTGCGCG	250	0.0	9.508493	18
CGCGCCA	260	0.0	9.498827	10
GTATTAG	505	0.0	9.399739	1
GTATAAC	465	0.0	9.391653	1
TCAACTC	1265	0.0	9.3903885	5
>>END_MODULE