FastQCFastQC Report
Thu 26 May 2016
SRR1364299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4495078
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT292800.6513791306847179No Hit
TATCAACGCAGAGTACTTTTTTTTT207770.4622166734370349No Hit
GGTATCAACGCAGAGTACTTTTTTT204220.4543191464085829No Hit
ACGCAGAGTACTTTTTTTTTTTTTT103930.2312084462160612No Hit
GTGGTATCAACGCAGAGTACATGGG64310.14306759526753485No Hit
GAGTACTTTTTTTTTTTTTTTTTTT55830.12420251661928892No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53230.11841841231676067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA5500.013.6475416
ACCGAGT5300.013.6245738
ACGGCCC3050.013.389976515
GTGGTAT20450.013.334261
TGGTATC19750.013.2757632
AGGACCG6600.012.95975
GGTATCA60200.012.8471931
CGAGTGG5900.011.91667810
CCGAGTG5850.011.856259
GGGCGAA4200.011.30873511
AGGGCGA3950.011.30514710
TCGACGG600.00588992811.08046414
GCCCCCG4100.010.88730418
CTCGACG700.001495717910.85421213
TGCCGTA1158.8582055E-710.73635115
GACCGAG7100.010.7058627
TGGTCGG2850.010.66794110
TATAACG1451.4462785E-810.4826112
CGGCCCC4350.010.48004516
CGCGCCA3000.010.45124910
Produced by FastQC (version 0.11.5)