##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364299_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4495078
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.698312910254284	31.0	30.0	34.0	26.0	34.0
2	29.73569023718832	31.0	30.0	34.0	26.0	34.0
3	29.768831152651856	31.0	30.0	34.0	26.0	34.0
4	33.14471695485596	35.0	33.0	37.0	27.0	37.0
5	32.721493820574416	35.0	33.0	37.0	26.0	37.0
6	32.638626293025396	35.0	33.0	37.0	25.0	37.0
7	32.37032349605502	35.0	32.0	37.0	23.0	37.0
8	32.26394469684397	35.0	32.0	37.0	23.0	37.0
9	33.028036220951	37.0	32.0	39.0	17.0	39.0
10	33.13674534679932	37.0	32.0	39.0	18.0	39.0
11	33.08178856963105	37.0	32.0	39.0	17.0	39.0
12	33.03293424496749	37.0	32.0	39.0	17.0	39.0
13	33.09183733852894	37.0	32.0	39.0	17.0	39.0
14	34.0287085118434	38.0	33.0	41.0	17.0	41.0
15	34.064315235464214	38.0	33.0	41.0	17.0	41.0
16	34.00447956631676	38.0	33.0	41.0	17.0	41.0
17	33.95974285651995	38.0	33.0	41.0	17.0	41.0
18	33.89637265471256	38.0	33.0	41.0	17.0	41.0
19	33.79115112129311	39.0	33.0	41.0	18.0	41.0
20	33.703396915470655	39.0	33.0	41.0	16.0	41.0
21	33.64006920458333	38.0	33.0	41.0	11.0	41.0
22	33.59307669410853	38.0	33.0	41.0	10.0	41.0
23	33.34027863365218	38.0	32.0	41.0	2.0	41.0
24	33.26166175536887	38.0	32.0	41.0	2.0	41.0
25	33.099899490064466	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.3007853586409155
1101	2	0.5490459079460628
1101	3	-1.67724403095497
1101	4	-0.5121388936164948
1101	5	0.4418988005091222
1101	6	0.8176242223691119
1101	7	0.9889660392687247
1101	8	1.0285155028573385
1101	9	1.834807649588889
1101	10	1.4906517770366179
1101	11	1.4450898326824912
1101	12	1.4618551635101653
1101	13	1.2900376545452374
1101	14	1.4700798397990837
1101	15	1.409292097846901
1101	16	1.3787229140949506
1101	17	1.4277918359675255
1101	18	1.372864562849955
1101	19	1.3043002668224233
1101	20	1.639802897423749
1101	21	1.4250262741605582
1101	22	1.6229627386555663
1101	23	1.9037594962523983
1101	24	1.8416086813715395
1101	25	1.9430711801581282
1102	1	0.6868441731605515
1102	2	0.6646345978046
1102	3	0.7245280895556867
1102	4	0.667800304282899
1102	5	0.8435801959968998
1102	6	1.061662309992819
1102	7	1.1100612406161474
1102	8	1.2214183759662873
1102	9	1.749626580852528
1102	10	1.375309639723568
1102	11	1.4431870116078187
1102	12	1.549441831098214
1102	13	1.3814129866079057
1102	14	1.4490884592268785
1102	15	1.4748524074572842
1102	16	1.4896229575302655
1102	17	1.5694893446451772
1102	18	1.6328412731510795
1102	19	1.561447175008901
1102	20	1.7223327141208458
1102	21	1.8468759174871394
1102	22	1.7588733931375913
1102	23	2.1957444828487525
1102	24	2.1556438313986632
1102	25	1.933992125405311
1103	1	-2.352409333853476
1103	2	-2.249763643121696
1103	3	-2.2873922789591212
1103	4	-2.1475331403842155
1103	5	-5.024650710260062
1103	6	-9.536963413676741
1103	7	-10.519318351902225
1103	8	-11.312607314303136
1103	9	-11.769765675883164
1103	10	-9.555224344912371
1103	11	-12.424446867261032
1103	12	-11.788923093194818
1103	13	-12.458007521074126
1103	14	-12.595465927324
1103	15	-12.559844764664735
1103	16	-6.691058544362406
1103	17	-4.959234770251282
1103	18	-4.337397120302494
1103	19	-4.007804522831222
1103	20	-8.694493299016543
1103	21	-5.6372910275139745
1103	22	-11.117465395550902
1103	23	-12.939178140175073
1103	24	-13.816288227162904
1103	25	-14.600694609174958
1104	1	0.2900350058538592
1104	2	0.5047830696292976
1104	3	0.5610180548530117
1104	4	0.7380396967944662
1104	5	0.7587192810617225
1104	6	0.7385657892738493
1104	7	0.658623451670806
1104	8	0.9023267948329305
1104	9	1.2681804855017589
1104	10	1.0104416147788058
1104	11	1.0018415862207277
1104	12	0.946062561561746
1104	13	0.9193097874813958
1104	14	0.976459477410117
1104	15	0.9518222012508204
1104	16	0.6002541669821184
1104	17	0.5862360978697367
1104	18	0.824939321478638
1104	19	0.9647529516578146
1104	20	1.30660410275501
1104	21	1.0962287752810127
1104	22	0.8428336288977363
1104	23	1.3236615073097369
1104	24	1.5470527557200597
1104	25	1.6075858880270673
1105	1	0.30984283467192597
1105	2	0.374539330855999
1105	3	-0.053907923320956286
1105	4	0.35209683345579634
1105	5	0.48066734013234225
1105	6	0.4869269381062651
1105	7	0.6854136803158681
1105	8	0.482748857388998
1105	9	0.6027839764643517
1105	10	0.17002907998092098
1105	11	0.789647687330735
1105	12	1.0093158404414169
1105	13	0.6984963340821437
1105	14	0.45296017070711514
1105	15	0.44361454966818314
1105	16	0.6707861651240279
1105	17	0.7483929318002112
1105	18	0.7174807710647073
1105	19	0.8455166770861666
1105	20	0.9722701768198903
1105	21	0.955882420097204
1105	22	0.7669745741585885
1105	23	1.2143655057012879
1105	24	1.1939498803684216
1105	25	1.3369021999948032
1106	1	-0.02083135898281796
1106	2	0.19067735219734772
1106	3	-1.1785791945584378E-4
1106	4	-1.3135223002758138
1106	5	-0.2260242012676983
1106	6	-0.009520539816186613
1106	7	0.24798116768388212
1106	8	0.3921960547532777
1106	9	0.9364927929515261
1106	10	0.4903572330625039
1106	11	0.5226691095805549
1106	12	0.7174939738167581
1106	13	0.578346876560758
1106	14	-0.0151736755378451
1106	15	0.5625529934037559
1106	16	0.352921653962305
1106	17	0.34573566581671855
1106	18	0.39360574370341794
1106	19	-1.0713246324641332
1106	20	0.17817007250354067
1106	21	-0.04203203515132259
1106	22	0.5090435968117504
1106	23	0.4923012882555753
1106	24	0.5373664599521462
1106	25	0.7419890875483688
1107	1	0.06306174090856231
1107	2	-0.05776621528076831
1107	3	-0.1964873448803246
1107	4	-0.07724350295082871
1107	5	0.04923725449982186
1107	6	0.0021046214709627975
1107	7	-0.0658800144103111
1107	8	-0.20984657515395355
1107	9	0.6115939681731462
1107	10	0.4451380869331132
1107	11	0.41520038499538714
1107	12	0.18114619722812364
1107	13	0.1924002508508309
1107	14	0.27155864680655384
1107	15	0.15940869311797456
1107	16	-0.039460475021925845
1107	17	-0.21244611855550488
1107	18	-0.13698963716785073
1107	19	0.0894884530182054
1107	20	0.07518150383593536
1107	21	0.09374796302910937
1107	22	0.018264127290485987
1107	23	0.1916072924713248
1107	24	0.45026475238304187
1107	25	0.13993366378194594
1108	1	-0.0017089942949013448
1108	2	0.1086003448051116
1108	3	0.22694758616066935
1108	4	0.058029489308296434
1108	5	0.2136298028506758
1108	6	0.1990150573664593
1108	7	-0.15445205309067944
1108	8	0.28905098130146456
1108	9	0.4293211709381879
1108	10	0.2733781593321112
1108	11	0.43683520215983407
1108	12	0.4300361833567763
1108	13	0.0017760103350425993
1108	14	0.20596894345509753
1108	15	-0.15602944680018282
1108	16	-0.6464708486446185
1108	17	-0.6955109445580163
1108	18	-0.7522510393743644
1108	19	-0.584084352108718
1108	20	0.07913137085706978
1108	21	-0.12745208203198644
1108	22	-0.024593767556183366
1108	23	-0.3272063424010696
1108	24	-0.014561548234397037
1108	25	-0.20550089572466845
1109	1	0.061287719553522635
1109	2	0.12952579825338262
1109	3	0.06594757678059793
1109	4	0.09391099712345863
1109	5	0.05852915468776132
1109	6	0.24622987975481436
1109	7	0.28918763336481135
1109	8	0.14200632903633448
1109	9	1.0787360890032645
1109	10	0.38518196263827775
1109	11	0.3104249866370665
1109	12	0.4323495433920428
1109	13	0.4433506741376618
1109	14	0.04437830226470396
1109	15	-0.14885642826203593
1109	16	0.17747084483341524
1109	17	0.1603324489015705
1109	18	0.07144841376749156
1109	19	0.3105445410116303
1109	20	0.5235449913456449
1109	21	0.4912272352239029
1109	22	0.19023104398255697
1109	23	0.5369805461355455
1109	24	0.35162345178123644
1109	25	0.3154329997875962
1110	1	0.034267241245608204
1110	2	0.08991621201018773
1110	3	0.038528949003158175
1110	4	0.20642852423242886
1110	5	0.09457506533102844
1110	6	0.18295894179681227
1110	7	0.2293021551361747
1110	8	0.0872000691873609
1110	9	-3.173468098324058
1110	10	-1.3674873112920345
1110	11	-0.3767967032593873
1110	12	0.04498467296617292
1110	13	-0.31880258735812106
1110	14	-0.005209503926430159
1110	15	0.130243386216776
1110	16	-0.3503643561705232
1110	17	-0.12467142461782288
1110	18	0.01279549620700493
1110	19	-0.23530341240344654
1110	20	0.1713990578236917
1110	21	-0.030292770031401517
1110	22	0.197221292982654
1110	23	0.3852803127427862
1110	24	0.4999551956879884
1110	25	0.5473745277664079
1111	1	0.0689482820405658
1111	2	0.08890449521531707
1111	3	0.09656421478628019
1111	4	-0.08618288284190356
1111	5	0.19163884088048633
1111	6	0.11729753187145775
1111	7	0.2183334141021902
1111	8	0.3749731676688981
1111	9	0.9209191107246326
1111	10	0.40831732175429636
1111	11	0.6687052666609716
1111	12	0.74597159039606
1111	13	0.4850112427227842
1111	14	0.4056608202845382
1111	15	0.44535207124402376
1111	16	0.46582381398932426
1111	17	0.46005330921892096
1111	18	0.38856176490722305
1111	19	0.5364720592144039
1111	20	0.6522805236471854
1111	21	0.6357728061872763
1111	22	0.6933329856117183
1111	23	0.9483310577645838
1111	24	0.8716141826972859
1111	25	1.0115317521201135
1112	1	-0.10109388361206584
1112	2	-0.1948204018210511
1112	3	-0.186448563041683
1112	4	-0.06270661965732671
1112	5	-0.05943055057006319
1112	6	-0.09033119090763364
1112	7	-0.01692036521493634
1112	8	-0.4193094209766812
1112	9	0.18314496529873026
1112	10	0.04153522359651163
1112	11	0.17726517120831886
1112	12	-0.3956652395564788
1112	13	-0.22702441977566679
1112	14	-0.9255848549634891
1112	15	-0.3310423615021847
1112	16	-0.4066544423451859
1112	17	-0.37321780123613735
1112	18	-0.42189721991342566
1112	19	-0.06681838437593512
1112	20	-0.074167932604567
1112	21	-0.35209565336749904
1112	22	0.004422251131082078
1112	23	0.12170074074494153
1112	24	-0.015012279491848801
1112	25	0.06522619622825232
1113	1	-2.494806885077864
1113	2	-2.4449889471363555
1113	3	-2.5544488548378474
1113	4	-2.4974311080670404
1113	5	-2.5071386329653755
1113	6	-2.4609675574381953
1113	7	-2.7249499111226143
1113	8	-2.863126325630809
1113	9	-11.968788560274305
1113	10	-5.2490102644526
1113	11	-3.716202031345656
1113	12	-13.405211303256454
1113	13	-5.815172688490421
1113	14	-4.840619392258198
1113	15	-4.15977396673037
1113	16	-4.034634152391725
1113	17	-4.079924425423695
1113	18	-4.26059958157186
1113	19	-5.007932063227688
1113	20	-15.97724515225659
1113	21	-17.988641393997398
1113	22	-12.948560264782952
1113	23	-19.01789919631367
1113	24	-20.20456544272179
1113	25	-16.62160863133055
1114	1	-0.19852161226723197
1114	2	-0.16895510490662247
1114	3	-0.14618262134106175
1114	4	-0.6500458599578707
1114	5	-0.21284606037035303
1114	6	-0.2168721728742824
1114	7	-0.33630290728067536
1114	8	-0.17204772350309128
1114	9	0.510153246897751
1114	10	-0.18414210441582668
1114	11	-0.2987339069815249
1114	12	-0.36438191357511585
1114	13	-0.2964518286623985
1114	14	-0.5018859912438813
1114	15	-0.3271832341297056
1114	16	-0.37132655101200385
1114	17	-0.4926115755451974
1114	18	-0.36572257434296773
1114	19	-0.7394695693623206
1114	20	-0.9961708437363086
1114	21	-1.1865889352246128
1114	22	-1.2543366621950263
1114	23	-0.9215548891164502
1114	24	-0.5247833224792018
1114	25	-0.8182750423649949
1115	1	-0.46231271549598674
1115	2	-0.4155245126869147
1115	3	-0.43707524997361347
1115	4	-0.3107275848286619
1115	5	-0.6331953962503967
1115	6	-0.6532828581211874
1115	7	-0.8692579654287975
1115	8	-0.5829122659939578
1115	9	-0.18046917829862252
1115	10	-0.65463557679643
1115	11	-0.4605277851354259
1115	12	-0.6318427791851562
1115	13	-0.7611476176924867
1115	14	-0.9796748645220674
1115	15	-0.9897964554859939
1115	16	-0.8146243656184495
1115	17	-0.7312758330532105
1115	18	-0.7897672893846206
1115	19	-0.6887374938457143
1115	20	-1.1720748207556682
1115	21	-0.6093622835526205
1115	22	-1.2281236081112894
1115	23	-0.9141177537016425
1115	24	-0.38761974890203277
1115	25	-0.3415611550686819
1116	1	-0.9798468056513236
1116	2	-0.9004101439059831
1116	3	-0.8731003007258096
1116	4	-0.7120260925318789
1116	5	-1.849171542250435
1116	6	-1.5627304450912547
1116	7	-2.8843811218371194
1116	8	-2.04448575227989
1116	9	-0.9388842511641116
1116	10	-2.028336613654716
1116	11	-1.713487376296463
1116	12	-2.763486429858691
1116	13	-2.3793758895392862
1116	14	-1.8963513406332666
1116	15	-3.244805227932101
1116	16	-3.5725635052554203
1116	17	-4.373113452646692
1116	18	-3.3313346646201616
1116	19	-2.875719403361529
1116	20	-3.288301382022194
1116	21	-2.449199453780114
1116	22	-2.258692377222186
1116	23	-2.373804206485051
1116	24	-2.9913769296489896
1116	25	-2.5796997849415213
1201	1	0.7026942831582055
1201	2	0.7927326737201099
1201	3	0.41800274915314617
1201	4	0.8319811713162011
1201	5	0.9945937913109191
1201	6	1.1118984246701942
1201	7	1.218962721926303
1201	8	1.2075605606649162
1201	9	2.0096575434225983
1201	10	1.6363395785531054
1201	11	1.625218923715984
1201	12	1.792196352001291
1201	13	1.579112512299794
1201	14	1.6759222286915758
1201	15	1.6673102587120425
1201	16	1.559917915370903
1201	17	1.5287686573408337
1201	18	1.5404152810414828
1201	19	1.62428626138081
1201	20	1.8523629093600107
1201	21	1.864580148390658
1201	22	1.9313505442557712
1201	23	2.213254811312474
1201	24	2.130557551862637
1201	25	2.287170371131417
1202	1	0.9458533105113212
1202	2	1.0255499769499892
1202	3	1.0539910510554833
1202	4	1.1411217779750018
1202	5	1.174124678031454
1202	6	1.2529339545696416
1202	7	1.286257945721566
1202	8	1.334845974559201
1202	9	1.9956975359846183
1202	10	1.6270797669952373
1202	11	1.800086021362361
1202	12	1.8190787818881518
1202	13	1.715238529636082
1202	14	1.7692833425551981
1202	15	1.805132667965026
1202	16	1.715417544181335
1202	17	1.7052415340099714
1202	18	1.7485559362486782
1202	19	1.8674464561944006
1202	20	2.03773282271451
1202	21	2.0669644152047297
1202	22	2.0440987223304248
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2211	4	0.15337529787511528
2211	5	-0.5548638757117956
2211	6	0.17734310830249456
2211	7	0.3473193220540338
2211	8	0.2906447013805362
2211	9	0.8527167904300654
2211	10	0.18514800432553358
2211	11	0.4421466273269985
2211	12	0.5720354610353553
2211	13	0.4860365478456856
2211	14	0.35810569847675566
2211	15	0.329425171275183
2211	16	0.4773152407049679
2211	17	0.3890090998613758
2211	18	0.36340432104939424
2211	19	0.13823521098808556
2211	20	0.4274534924568414
2211	21	0.7325165161131508
2211	22	0.5471441692823262
2211	23	0.9854049170372079
2211	24	1.0308532296517612
2211	25	1.167168351947197
2212	1	-0.037044888233872086
2212	2	0.06327252234043357
2212	3	0.01509226037926581
2212	4	0.16153686960461755
2212	5	-0.09211207696785806
2212	6	0.18094469637672717
2212	7	0.1661269592206338
2212	8	0.04298698471560414
2212	9	0.6912126791905777
2212	10	0.29610817315567317
2212	11	0.40508740326015413
2212	12	0.1885460222056139
2212	13	0.06011560601912436
2212	14	0.08864264250971843
2212	15	0.28614354252135143
2212	16	0.20857660605771144
2212	17	0.01520556114304128
2212	18	0.08721012013466378
2212	19	0.2670711675736186
2212	20	0.48792139191711925
2212	21	0.6217778807650092
2212	22	0.28800422663606895
2212	23	0.7371358324437622
2212	24	0.7452193158772289
2212	25	0.8235123433486891
2213	1	-0.3256220009733468
2213	2	-0.20444205538848337
2213	3	-0.2930670372490205
2213	4	-0.34728293266459787
2213	5	-0.3564336999118467
2213	6	-0.156229172042174
2213	7	-0.1739056507918697
2213	8	-0.07865105816596696
2213	9	0.385982728452511
2213	10	-0.06869661434359386
2213	11	0.08703577870920753
2213	12	-0.02087532712487672
2213	13	-0.3029967574212833
2213	14	-0.15920421949108032
2213	15	-0.20248204844750006
2213	16	-0.31121088781735295
2213	17	-0.36537571745272857
2213	18	-0.4743483156344652
2213	19	-0.37830479664344097
2213	20	-0.022688168342021697
2213	21	-0.22421082885848875
2213	22	-0.09849209696339045
2213	23	-0.4011248506516978
2213	24	-0.2682155347390065
2213	25	0.08166739890722141
2214	1	-0.849903898332343
2214	2	-0.6949809507268974
2214	3	-0.6942912858441126
2214	4	-0.5671355546615189
2214	5	-0.8520106323509253
2214	6	-0.7070025097948722
2214	7	-0.6645869006180831
2214	8	-0.6978718146881526
2214	9	-10.692717871607222
2214	10	-3.917771481072176
2214	11	-1.7359970665634705
2214	12	-1.140269375524845
2214	13	-0.8331009440935588
2214	14	-0.9317817197776819
2214	15	-0.8792552371021145
2214	16	-1.129943611400499
2214	17	-1.0642082720857715
2214	18	-1.3004249603622853
2214	19	-1.4590200129340403
2214	20	-1.0734121115816535
2214	21	-1.659365754605517
2214	22	-1.5221371127226178
2214	23	-1.8355606876226425
2214	24	-2.2379225921128523
2214	25	-9.67073144049262
2215	1	-0.551142814029685
2215	2	-0.29907225790388026
2215	3	-0.2102095207603938
2215	4	-0.3640957933455482
2215	5	-0.4290437596971657
2215	6	-0.29037598097972506
2215	7	-0.43637965880384044
2215	8	-0.5325488830745542
2215	9	0.31219253508110256
2215	10	0.31784375347235994
2215	11	0.378217719131527
2215	12	0.2969050394532502
2215	13	0.13980819111382914
2215	14	-0.032816025219247535
2215	15	-0.39375323260404116
2215	16	-0.13854233046128428
2215	17	-0.19065914616904678
2215	18	-0.20230626127165863
2215	19	-0.13410928298785052
2215	20	0.054119341707881574
2215	21	0.04077737679342164
2215	22	-0.036047138086388486
2215	23	0.3536938617828298
2215	24	0.4665296264836982
2215	25	0.5036265484363938
2216	1	-0.757292341310464
2216	2	-0.7692000140531334
2216	3	-0.8155122635949823
2216	4	-0.7338181990028119
2216	5	-1.6934434020182252
2216	6	-2.300381653455183
2216	7	-1.492061071061407
2216	8	-2.491332567762516
2216	9	-1.1140768462000388
2216	10	-1.5943359282016658
2216	11	-1.1205372074250448
2216	12	-2.3109182172822678
2216	13	-1.978987645553019
2216	14	-2.1472458922898454
2216	15	-1.7977173695225446
2216	16	-2.88427758855633
2216	17	-3.221491830905382
2216	18	-2.7835568804457615
2216	19	-4.246635857358456
2216	20	-2.905916891778759
2216	21	-3.240418535196042
2216	22	-3.0604015609747357
2216	23	-2.6617220796579772
2216	24	-2.8227088107066365
2216	25	-2.7961142972927036
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	150777.0
3	6.0
4	2202.0
5	21335.0
6	27808.0
7	22477.0
8	18206.0
9	15128.0
10	13177.0
11	11810.0
12	10655.0
13	10023.0
14	9944.0
15	10138.0
16	10682.0
17	11743.0
18	12890.0
19	15760.0
20	20452.0
21	27186.0
22	33857.0
23	41044.0
24	48119.0
25	54956.0
26	62097.0
27	68341.0
28	77177.0
29	90841.0
30	110622.0
31	136827.0
32	168134.0
33	208198.0
34	256654.0
35	320447.0
36	414500.0
37	593442.0
38	1387423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00942828780077	18.720700019604713	13.47433595983103	24.795535732763483
2	16.7868402125282	19.641372565726524	38.16444261625125	25.407344605494025
3	20.12715719858173	23.66892944397257	28.25885092677171	27.94506243067399
4	13.205956691912254	15.924430984284227	34.848698895261776	36.020913428541746
5	14.106814815968702	37.62601351013038	33.26774470282053	14.999426971080398
6	34.10639433987452	35.64421750545099	16.136697370177547	14.112690784496952
7	29.917115946446888	31.493604021156013	19.60876501647668	18.980515015920414
8	27.300338481579335	35.65483201313427	18.061689497279975	18.983140008006423
9	26.39687838608608	15.653044915482624	18.642269748371728	39.307806950059565
10	15.894743635679806	30.040347976258563	30.651198087866455	23.413710300195177
11	36.0928064621086	22.686592807129756	21.020346757929527	20.200253972832115
12	23.73981923808032	25.37735118645033	28.80092160813322	22.08190796733613
13	29.383173079062235	21.06922450577761	25.15724347822218	24.39035893693798
14	22.38311571294131	20.487070701227854	26.652954616748577	30.476858969082254
15	24.676241178379993	28.6105151730418	22.79915306218479	23.914090586393417
16	24.326547956878244	28.02186568002643	24.2317699787417	23.419816384353627
17	22.911675076307507	27.852245439737928	25.85967339341523	23.376406090539337
18	22.623131517245156	26.728820747139455	27.696064143509442	22.951983592105947
19	24.11321401235337	26.114250876662098	27.322843055234163	22.44969205575037
20	23.91454631096266	25.572449871629455	27.85475115649192	22.65825266091597
21	24.46573428440477	25.0724650579142	27.542595748657273	22.919204909023758
22	23.971313220668115	24.980832614324612	27.694525047089698	23.35332911791758
23	23.33015923163007	25.13183275450474	27.98563199850491	23.55237601536028
24	23.642879406535823	25.351131266617706	27.79131308443547	23.214676242411
25	23.50928234165948	25.0663088032703	27.841034359429123	23.583374495641102
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	735.0
1	735.0
2	1347.5
3	1960.0
4	1960.0
5	1960.0
6	4886.5
7	7813.0
8	7813.0
9	7813.0
10	10620.0
11	13427.0
12	13427.0
13	13427.0
14	17023.5
15	20620.0
16	20620.0
17	20620.0
18	33917.5
19	47215.0
20	47215.0
21	47215.0
22	70404.5
23	93594.0
24	93594.0
25	93594.0
26	131055.0
27	168516.0
28	168516.0
29	168516.0
30	208040.0
31	247564.0
32	247564.0
33	247564.0
34	285773.0
35	323982.0
36	323982.0
37	323982.0
38	361858.0
39	399734.0
40	399734.0
41	399734.0
42	439427.0
43	479120.0
44	479120.0
45	479120.0
46	518731.5
47	558343.0
48	558343.0
49	558343.0
50	572985.5
51	587628.0
52	587628.0
53	587628.0
54	558214.5
55	528801.0
56	528801.0
57	528801.0
58	474846.0
59	420891.0
60	420891.0
61	420891.0
62	354063.5
63	287236.0
64	287236.0
65	287236.0
66	227252.5
67	167269.0
68	167269.0
69	167269.0
70	125946.5
71	84624.0
72	84624.0
73	84624.0
74	58288.0
75	31952.0
76	31952.0
77	31952.0
78	23752.0
79	15552.0
80	15552.0
81	15552.0
82	10610.5
83	5669.0
84	5669.0
85	5669.0
86	3843.5
87	2018.0
88	2018.0
89	2018.0
90	1383.5
91	749.0
92	749.0
93	749.0
94	405.5
95	62.0
96	62.0
97	62.0
98	33.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028052905867261925
2	0.0011345743054959224
3	2.6695866011668763E-4
4	0.003715174686623903
5	0.03143438222873997
6	0.010633853294648058
7	0.018865078648245926
8	0.026362167686522903
9	0.03348106528963457
10	0.03710725375621958
11	0.037463198636375164
12	0.007252376933170013
13	0.003070024591341908
14	0.005517145642411544
15	0.006607226837888018
16	0.0080754994685298
17	0.009677251429229925
18	0.014593740086378924
19	0.016862888697370768
20	0.014838452191485888
21	0.011078784394842537
22	0.010322401524511922
23	0.00818673224357842
24	0.005539392197421268
25	0.10509272586593603
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4495078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.34225680468687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.13641949859166	29.409401230767106
2	13.783921399426417	11.397168365414116
3	5.607489696267871	6.954788371582257
4	2.7712483807191206	4.582786489010501
5	1.5925133378158387	3.2919047688435734
6	1.0108345215849488	2.5074108227044647
7	0.7151355624097937	2.069572264991691
8	0.5135728060544067	1.6985807068643943
9	0.38200749746890056	1.421374685550755
>10	2.16713115878333	16.655975788731077
>50	0.1907399780914978	5.490109587358786
>100	0.11974618142474595	8.985475589219282
>500	0.006631045613810092	1.8581460743961473
>1k	0.002228465951120631	1.4704816763854567
>5k	1.6305848422833635E-4	0.3895742790876285
>10k+	2.1741131230444845E-4	1.8172492990929625
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	29280	0.6513791306847179	No Hit
TATCAACGCAGAGTACTTTTTTTTT	20777	0.4622166734370349	No Hit
GGTATCAACGCAGAGTACTTTTTTT	20422	0.4543191464085829	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	10393	0.2312084462160612	No Hit
GTGGTATCAACGCAGAGTACATGGG	6431	0.14306759526753485	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	5583	0.12420251661928892	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5323	0.11841841231676067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.673966502917191E-5
2	0.0	0.0	0.0	2.224655500972397E-5	6.673966502917191E-5
3	0.0	0.0	0.0	6.673966502917191E-5	6.673966502917191E-5
4	0.0	0.0	0.0	6.673966502917191E-5	6.673966502917191E-5
5	2.224655500972397E-5	0.0	0.0	6.673966502917191E-5	6.673966502917191E-5
6	2.224655500972397E-5	0.0	0.0	6.673966502917191E-5	2.0021899508751572E-4
7	6.673966502917191E-5	0.0	0.0	6.673966502917191E-5	2.0021899508751572E-4
8	6.673966502917191E-5	0.0	0.0	6.673966502917191E-5	2.2246555009723968E-4
9	6.673966502917191E-5	0.0	0.0	8.898622003889588E-5	2.2246555009723968E-4
10	6.673966502917191E-5	0.0	0.0	8.898622003889588E-5	2.4471210510696367E-4
11	6.673966502917191E-5	0.0	0.0	8.898622003889588E-5	2.4471210510696367E-4
12	6.673966502917191E-5	0.0	0.0	1.1123277504861984E-4	4.226845451847554E-4
13	8.898622003889588E-5	0.0	0.0	1.1123277504861984E-4	4.6717765520420333E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGA	550	0.0	13.647541	6
ACCGAGT	530	0.0	13.624573	8
ACGGCCC	305	0.0	13.3899765	15
GTGGTAT	2045	0.0	13.33426	1
TGGTATC	1975	0.0	13.275763	2
AGGACCG	660	0.0	12.9597	5
GGTATCA	6020	0.0	12.847193	1
CGAGTGG	590	0.0	11.916678	10
CCGAGTG	585	0.0	11.85625	9
GGGCGAA	420	0.0	11.308735	11
AGGGCGA	395	0.0	11.305147	10
TCGACGG	60	0.005889928	11.080464	14
GCCCCCG	410	0.0	10.887304	18
CTCGACG	70	0.0014957179	10.854212	13
TGCCGTA	115	8.8582055E-7	10.736351	15
GACCGAG	710	0.0	10.705862	7
TGGTCGG	285	0.0	10.667941	10
TATAACG	145	1.4462785E-8	10.482611	2
CGGCCCC	435	0.0	10.480045	16
CGCGCCA	300	0.0	10.451249	10
>>END_MODULE