FastQCFastQC Report
Thu 26 May 2016
SRR1364298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593867
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN145420.25996327763960064No Hit
GTATCAACGCAGAGTACTTTTTTTT96020.17165227560826885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.012.1986371
CGCGCCA2700.011.25812610
GGACCGA3850.011.1033766
AGGACCG5050.011.1032755
CGCGAAT700.001480446510.86765916
GTATCAA70800.010.8071911
TGGTATC11600.010.0661182
GTGGTAT13600.09.6327711
CGCATAT1502.6557245E-79.4992148
GTCTTAG7600.09.4931651
CAGGACC15750.09.2322844
CCTACAC10750.09.095943
GTTCGCG3800.09.00871716
GTATAAT10150.08.9787571
CATGGGT32250.08.9291724
GTATTGG6700.08.9264091
TTGCGCG3450.08.819653518
GTTATCA7450.08.7923271
TAAGGCT7800.08.77275854
TGCACCG3150.08.74939255
Produced by FastQC (version 0.11.5)