FastQCFastQC Report
Thu 26 May 2016
SRR1364298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593867
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT187020.3343304372449327No Hit
GGTATCAACGCAGAGTACTTTTTTT134720.2408351861064984No Hit
TATCAACGCAGAGTACTTTTTTTTT127980.2287862761127499No Hit
ACGCAGAGTACTTTTTTTTTTTTTT75090.13423629843183615No Hit
GTGGTATCAACGCAGAGTACATGGG59250.10591957227442125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.013.2125091
TCGATAC604.092431E-412.6676023
TGGTATC15700.011.9196642
CGTGCCG1001.9306262E-611.40084310
CGAGTGG4300.011.26827410
ACCGAGT4600.010.9469518
AGGACCG6350.010.9249985
TAGGCCG700.001488197110.8608595
CGATACT1553.6634447E-910.420594
GTGGTAT19200.010.1950111
GTCGGTT2053.6379788E-1210.19186512
CTACCGA750.002646806410.1345354
TTAGCGC856.5914594E-410.0600184
GGACCGA5150.09.7779466
ACGTCGC1106.852433E-59.49883411
CAGGACC17050.09.36181454
CGGTTTG2550.09.31158314
GTATCAA92900.09.297581
ACCGGTC1252.753166E-59.1210818
GACGGGT1252.753166E-59.1210818
Produced by FastQC (version 0.11.5)