##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364298_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5593867
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.634101954873078	31.0	30.0	34.0	26.0	34.0
2	29.683501055709762	31.0	30.0	34.0	26.0	34.0
3	29.728506594811783	31.0	30.0	34.0	26.0	34.0
4	33.113341808090894	35.0	33.0	37.0	27.0	37.0
5	32.66264678799121	35.0	33.0	37.0	26.0	37.0
6	32.578558982542845	35.0	33.0	37.0	25.0	37.0
7	32.3070954314788	35.0	32.0	37.0	23.0	37.0
8	32.20275347983783	35.0	32.0	37.0	22.0	37.0
9	32.911782314452594	37.0	32.0	39.0	17.0	39.0
10	33.060551135734904	37.0	32.0	39.0	17.0	39.0
11	33.03854954005878	37.0	32.0	39.0	17.0	39.0
12	32.900433635622726	37.0	32.0	39.0	17.0	39.0
13	33.03316113879718	37.0	32.0	39.0	17.0	39.0
14	33.98326220483969	38.0	33.0	41.0	17.0	41.0
15	34.01560941652706	38.0	33.0	41.0	17.0	41.0
16	33.94105902053088	38.0	33.0	41.0	17.0	41.0
17	33.864658741439506	38.0	33.0	41.0	17.0	41.0
18	33.79247057536406	38.0	33.0	41.0	17.0	41.0
19	33.66728043408969	38.0	32.0	41.0	16.0	41.0
20	33.48192761822904	38.0	32.0	41.0	10.0	41.0
21	33.40143106727421	38.0	32.0	40.0	10.0	41.0
22	33.39403779174585	38.0	32.0	41.0	10.0	41.0
23	33.09409126101854	38.0	32.0	40.0	2.0	41.0
24	33.00026993848799	38.0	32.0	41.0	2.0	41.0
25	32.85643473468354	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2677809650815455
1101	2	0.5313316373558195
1101	3	-1.4612093345564858
1101	4	-0.44429654204316193
1101	5	0.4464346592105244
1101	6	0.8304225827224414
1101	7	1.0032122536147767
1101	8	1.1214247490616955
1101	9	1.854478739439628
1101	10	1.4929605742385306
1101	11	1.4854035132151253
1101	12	1.5998653811702042
1101	13	1.35297088575404
1101	14	1.5247015616679178
1101	15	1.5110883018964998
1101	16	1.5087999833065666
1101	17	1.4574509734575543
1101	18	1.4845237178658977
1101	19	1.5122657975125975
1101	20	1.7828540615793358
1101	21	1.669241723600372
1101	22	1.7180295443880738
1101	23	2.003117650049383
1101	24	1.969517690970143
1101	25	2.0838358694172214
1102	1	0.8346454052487324
1102	2	0.8050028193933834
1102	3	0.9073656857803414
1102	4	0.9805703219502746
1102	5	1.1264589081094414
1102	6	1.2235990459527812
1102	7	1.2280319770169257
1102	8	1.3146003932880816
1102	9	1.96302064223471
1102	10	1.5631257598907737
1102	11	1.5966520645354052
1102	12	1.6892683315415482
1102	13	1.5134978945899178
1102	14	1.6185605849216742
1102	15	1.6659447188031251
1102	16	1.6308599186541812
1102	17	1.5758060859142446
1102	18	1.6690127615075312
1102	19	1.6124128565863955
1102	20	1.8664604848678081
1102	21	1.9715068864481466
1102	22	1.9472534881495207
1102	23	2.216913048395888
1102	24	2.301053204258004
1102	25	2.021732607696741
1103	1	-3.5315250293773666
1103	2	-3.537437149539535
1103	3	-3.5248567744663184
1103	4	-3.6630858214278206
1103	5	-6.795998844183419
1103	6	-10.813735739003551
1103	7	-12.143646359368333
1103	8	-12.568066357502289
1103	9	-13.358843255392614
1103	10	-11.527396482025523
1103	11	-14.409357155813925
1103	12	-13.001872219605666
1103	13	-14.017230244985
1103	14	-13.986754957226257
1103	15	-14.465137620696673
1103	16	-9.499043466248935
1103	17	-7.273147356506016
1103	18	-6.480942633365224
1103	19	-6.186192548459463
1103	20	-11.626359899284065
1103	21	-8.13791482177172
1103	22	-13.413868790546445
1103	23	-14.684892823438712
1103	24	-16.981104793754326
1103	25	-17.63708609985013
1104	1	0.33238641576282646
1104	2	0.6159514074587129
1104	3	0.5461390400179376
1104	4	0.7434853702485853
1104	5	0.7973609507593622
1104	6	0.8540552301581599
1104	7	0.6767096233141672
1104	8	0.9541943458898672
1104	9	1.2157320207095097
1104	10	1.062126509150481
1104	11	0.9852637857618731
1104	12	0.9830736291908764
1104	13	0.9169300176935309
1104	14	1.036510328265578
1104	15	0.967386004236019
1104	16	0.6867573222532428
1104	17	0.6148913123716753
1104	18	0.9015343832349245
1104	19	1.0936831982944781
1104	20	1.3395416584743032
1104	21	1.135473181175442
1104	22	0.8128552317477897
1104	23	1.3189942823345007
1104	24	1.5527892813983257
1104	25	1.5036863737255217
1105	1	0.3588943266695246
1105	2	0.41479346507487946
1105	3	-0.021114905029744335
1105	4	0.3612565548814999
1105	5	0.5047622140070729
1105	6	0.5617658278075197
1105	7	0.6419045214166914
1105	8	0.5102002875244906
1105	9	0.6382725637811575
1105	10	0.29342799768083694
1105	11	0.8919482287726481
1105	12	1.0259608369867976
1105	13	0.8355741595643664
1105	14	0.634584135646314
1105	15	0.5426287683968241
1105	16	0.7858712018492895
1105	17	0.7624805539547879
1105	18	0.8207330429852959
1105	19	0.8850457940916314
1105	20	1.1148798165675657
1105	21	1.0508328529807756
1105	22	0.8816440123599563
1105	23	1.3568010803553463
1105	24	1.3125967505845537
1105	25	1.426187349256601
1106	1	-0.08144800741920832
1106	2	0.10955633383561647
1106	3	0.017923163584839585
1106	4	-1.618327254774087
1106	5	-0.2729818521707159
1106	6	0.046078022955683196
1106	7	0.4381482497471083
1106	8	0.5277932291339837
1106	9	1.0044777788549268
1106	10	0.5234216974655013
1106	11	0.7236363506502457
1106	12	0.8582509854112743
1106	13	0.5721503619450203
1106	14	0.18232292253917848
1106	15	0.65839290123062
1106	16	0.5162296693252699
1106	17	0.488773730243949
1106	18	0.5133087026608223
1106	19	-0.9240566334391005
1106	20	0.37019858194029354
1106	21	0.01310342909449247
1106	22	0.6701506463926705
1106	23	0.5319022800829174
1106	24	0.7220567455605007
1106	25	1.0149500756244123
1107	1	0.05106847329732389
1107	2	-0.06732566379402627
1107	3	-0.2035941863167423
1107	4	-0.039219668757901616
1107	5	0.1515135131756722
1107	6	0.11070079144406719
1107	7	0.05154625930134671
1107	8	-0.17374931766754287
1107	9	0.7005697127403323
1107	10	0.5676830028377182
1107	11	0.5365160624525345
1107	12	0.30979543218012395
1107	13	0.2759816027143458
1107	14	0.3669536135579605
1107	15	0.19476902222800163
1107	16	0.08883572716774069
1107	17	-0.08126622506881631
1107	18	-0.050064167325814424
1107	19	0.1914840325947793
1107	20	0.13931061987990745
1107	21	0.18115795189003592
1107	22	0.14133022210397428
1107	23	0.349945449740396
1107	24	0.5958131836164284
1107	25	0.1260830841366669
1108	1	-0.01671904057589302
1108	2	0.026275239795396033
1108	3	0.15416997868727478
1108	4	-0.01696620877409316
1108	5	0.21478747317419788
1108	6	0.20396262867178194
1108	7	-0.1793374223888904
1108	8	0.27916677389026034
1108	9	0.5225944711812573
1108	10	0.24116444669263615
1108	11	0.4469611745162254
1108	12	0.40675512511889167
1108	13	-0.018034053600132438
1108	14	0.053473929664704656
1108	15	-0.12917552989947723
1108	16	-0.720541249742908
1108	17	-0.7748719758971063
1108	18	-0.7313086578479542
1108	19	-0.6006818145480111
1108	20	0.00892036173506483
1108	21	-0.17000458090310389
1108	22	-0.11732549434297113
1108	23	-0.5508364980203879
1108	24	-0.15997784804358162
1108	25	-0.2957987141408793
1109	1	0.2151638872578694
1109	2	0.23631190297076188
1109	3	0.19698647886055198
1109	4	0.2741361399809037
1109	5	0.28081309249888875
1109	6	0.41975486770316195
1109	7	0.5411126145522331
1109	8	0.4555265914499671
1109	9	1.2894752828126244
1109	10	0.6105973632076669
1109	11	0.4585776554373808
1109	12	0.5273167777832555
1109	13	0.65194946026525
1109	14	0.17405563008014013
1109	15	-0.057985782633437566
1109	16	0.35074350187873904
1109	17	0.2459956392105198
1109	18	0.14951464623367627
1109	19	0.3777185918821928
1109	20	0.6891711114679566
1109	21	0.6485066095042171
1109	22	0.2840524273697511
1109	23	0.6178266450490142
1109	24	0.5028677242476718
1109	25	0.5755367823692197
1110	1	0.09030824887140554
1110	2	0.09987160775276394
1110	3	0.012099281504720949
1110	4	0.16913124889338604
1110	5	0.16451729495249623
1110	6	0.16481490932892484
1110	7	0.2462853197628121
1110	8	0.07032327417415019
1110	9	-3.3312413265362437
1110	10	-1.308595560832316
1110	11	-0.2990390328651884
1110	12	0.012505331257877117
1110	13	-0.3183034811901635
1110	14	-0.025015508481800453
1110	15	0.11712676790313026
1110	16	-0.36352679868554105
1110	17	-0.14234354145178685
1110	18	-0.009208459287762594
1110	19	-0.2408278662461356
1110	20	0.07095828323286213
1110	21	-0.0751751078527505
1110	22	0.21170071015684755
1110	23	0.4206331828793566
1110	24	0.48107997523639767
1110	25	0.5832333297556431
1111	1	0.11300697837625506
1111	2	0.13996447290610803
1111	3	0.0716086083699139
1111	4	-0.06300789051051936
1111	5	0.16168524534616324
1111	6	0.1103278264394092
1111	7	0.21320792714499248
1111	8	0.2560641640283521
1111	9	0.9258657263020424
1111	10	0.40305390476501657
1111	11	0.5659747485452442
1111	12	0.6278935881201306
1111	13	0.38007687840144655
1111	14	0.24561154817138942
1111	15	0.30976862393324467
1111	16	0.29046363393633357
1111	17	0.3236795218144124
1111	18	0.22105319716222738
1111	19	0.40314834081942763
1111	20	0.5533572678043441
1111	21	0.45590865964639704
1111	22	0.5481053502519515
1111	23	0.7762796253105151
1111	24	0.7490917760337936
1111	25	0.9691877962769553
1112	1	-0.1310154142661908
1112	2	-0.1587400540998516
1112	3	-0.1214578301354905
1112	4	-0.07475341915144895
1112	5	0.0017160576603671984
1112	6	-0.0579343062201616
1112	7	0.10884640706618853
1112	8	-0.4199246716802705
1112	9	0.24818266590793314
1112	10	0.0805553549362017
1112	11	0.16752975281829663
1112	12	-0.46080899272451603
1112	13	-0.14269808303217957
1112	14	-1.002697721082093
1112	15	-0.259097286248803
1112	16	-0.3951584764455731
1112	17	-0.34008754023351884
1112	18	-0.45174953922114724
1112	19	-0.12975844680732251
1112	20	-0.09726151749158873
1112	21	-0.31583088975831686
1112	22	-0.07374613202616587
1112	23	0.18228816584023377
1112	24	0.10243270534795101
1112	25	0.02516507537832524
1113	1	-2.3230358357274987
1113	2	-2.336837002372686
1113	3	-2.3619504110996985
1113	4	-2.3808755083312896
1113	5	-2.2612063676338217
1113	6	-2.3004985638793904
1113	7	-2.7794005643819197
1113	8	-2.7405780866249536
1113	9	-11.309079406120073
1113	10	-4.88904145109602
1113	11	-3.4740114548612304
1113	12	-12.953683544929092
1113	13	-5.363315738511478
1113	14	-4.458642701414387
1113	15	-3.956957188869236
1113	16	-3.6472202847726365
1113	17	-3.7501501837907227
1113	18	-3.896651345881118
1113	19	-4.599095304795448
1113	20	-15.19208658422217
1113	21	-17.239472180772893
1113	22	-12.438205896729272
1113	23	-18.53016155976728
1113	24	-19.609664384096018
1113	25	-16.201405101875714
1114	1	-0.017374981285346536
1114	2	0.013643676114931225
1114	3	0.028283060172064722
1114	4	-0.4739476176290651
1114	5	3.7791132325537546E-4
1114	6	0.07821349396445498
1114	7	-0.10628765895229364
1114	8	0.008204728667468686
1114	9	0.7451624574760842
1114	10	0.02595977903305169
1114	11	-0.08409733318499235
1114	12	-0.111111359954009
1114	13	-0.09077406246111508
1114	14	-0.049885163600436044
1114	15	-0.014980033668848591
1114	16	-0.0011327946516246357
1114	17	-0.02902008002298828
1114	18	-0.11349296976287349
1114	19	-0.3285439451000727
1114	20	-0.7289152100383873
1114	21	-0.7011894767589197
1114	22	-0.9028475585723257
1114	23	-0.5469961628638629
1114	24	-0.10562196317282968
1114	25	-0.3283308870053432
1115	1	-0.5114817932395574
1115	2	-0.5249409356711112
1115	3	-0.5285127423005314
1115	4	-0.34318665625745837
1115	5	-0.8175424277544785
1115	6	-0.9103283690633575
1115	7	-1.0912034878869612
1115	8	-0.7868832334537714
1115	9	-0.38469906960391853
1115	10	-0.7511917498064875
1115	11	-0.6204612115774566
1115	12	-0.7504894154728952
1115	13	-0.840734153856527
1115	14	-1.1257366709531382
1115	15	-1.0717751300258982
1115	16	-0.8427489853894414
1115	17	-0.8006844111035178
1115	18	-1.0086662724860105
1115	19	-0.9012361607358343
1115	20	-1.359146610902588
1115	21	-0.8363417707138794
1115	22	-1.274611901766658
1115	23	-1.1442869335047732
1115	24	-0.6363173813302723
1115	25	-0.6106470234088768
1116	1	-1.0171319941325159
1116	2	-0.9352772089043029
1116	3	-0.9689339225756832
1116	4	-0.8548930526665757
1116	5	-1.9533285185420404
1116	6	-1.7723777557950235
1116	7	-2.8749144589443922
1116	8	-2.0483493212377795
1116	9	-0.9594107594211998
1116	10	-2.1058059792539296
1116	11	-1.8151849180293418
1116	12	-2.680020535680516
1116	13	-2.450063928648717
1116	14	-1.9843104422715
1116	15	-3.1680903486375556
1116	16	-3.487478735657394
1116	17	-4.211420872805096
1116	18	-3.3787244224802286
1116	19	-2.8128129505326918
1116	20	-3.1717030855353485
1116	21	-2.5532331205299563
1116	22	-2.2860896469858467
1116	23	-2.3636916113040947
1116	24	-3.0172380196775848
1116	25	-2.591137952149367
1201	1	0.7721169033344069
1201	2	0.8169756592614839
1201	3	0.40067822837660927
1201	4	0.8383819449682974
1201	5	0.9798630702659139
1201	6	1.1281774556587223
1201	7	1.2916061233376155
1201	8	1.2215132977020602
1201	9	1.9693200351126094
1201	10	1.7001653130308725
1201	11	1.6278361342830294
1201	12	1.7035827132943098
1201	13	1.6308784872292819
1201	14	1.6562116137468195
1201	15	1.7279861825111027
1201	16	1.6656699956782575
1201	17	1.6145896743240513
1201	18	1.6109590601424486
1201	19	1.655152260138422
1201	20	1.8430455358329922
1201	21	1.9261110105185395
1201	22	1.9300610394654925
1201	23	2.2264992444489664
1201	24	2.243819950855311
1201	25	2.338916317881761
1202	1	0.9310120308251761
1202	2	0.9796648609665901
1202	3	0.9503399271180015
1202	4	1.087414687031
1202	5	1.1908657067087063
1202	6	1.1739136518775979
1202	7	1.3259222165415707
1202	8	1.3376523415714132
1202	9	2.025155185123289
1202	10	1.7314523885744393
1202	11	1.8209527188228734
1202	12	1.805319080104404
1202	13	1.7470694158077862
1202	14	1.8346605659037039
1202	15	1.817499936894876
1202	16	1.7367845642153839
1202	17	1.7926073291683622
1202	18	1.865243415709486
1202	19	1.8357836995101593
1202	20	2.049450448611495
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2211	5	-0.6381889122021995
2211	6	0.12614258389833566
2211	7	0.3459212307420998
2211	8	0.3047797162938437
2211	9	0.8464437271291132
2211	10	0.06565973911278178
2211	11	0.5807323714305497
2211	12	0.5871800655302124
2211	13	0.4958872683604909
2211	14	0.3944263363073688
2211	15	0.3164004277489525
2211	16	0.3653816072747702
2211	17	0.30802900896465957
2211	18	0.2502633119972941
2211	19	0.04753833831322396
2211	20	0.4631597458401373
2211	21	0.6463481691433728
2211	22	0.4967260151370425
2211	23	0.8936281451236994
2211	24	0.920256341837181
2211	25	1.1062698235088178
2212	1	0.10854301715989223
2212	2	0.1552143036373934
2212	3	0.21555435714394733
2212	4	0.3033712518636875
2212	5	0.061219804949054435
2212	6	0.2056344768308449
2212	7	0.2601297442486086
2212	8	0.09694341477456447
2212	9	0.5679966766521289
2212	10	0.27349688549081463
2212	11	0.3732405469879154
2212	12	0.2826540388958705
2212	13	-0.023131262602483105
2212	14	0.08726242407737317
2212	15	0.2706028988256364
2212	16	0.29430910358034623
2212	17	0.08891179286663231
2212	18	0.17190844124294102
2212	19	0.36683138078308986
2212	20	0.624929436554801
2212	21	0.7429834290306587
2212	22	0.3164113480567323
2212	23	0.7414105176188812
2212	24	0.9146584992234352
2212	25	0.8100825640577085
2213	1	-0.1254020396626423
2213	2	-0.11995252812868529
2213	3	-0.20987229929974305
2213	4	-0.21407735761209779
2213	5	-0.05117594886793597
2213	6	0.04651713168554039
2213	7	-0.012646358221317655
2213	8	-0.006438331822941734
2213	9	0.5873939080878401
2213	10	0.08561111464767635
2213	11	0.3637446795230517
2213	12	0.2013407957906992
2213	13	-0.04610333821773338
2213	14	-0.01070529611151727
2213	15	-0.02323949617913712
2213	16	-0.15751387730431787
2213	17	-0.1523422444943705
2213	18	-0.28962805218014864
2213	19	-0.20795461418565253
2213	20	0.11273847895294864
2213	21	-0.09171516564649096
2213	22	0.1274162550301554
2213	23	-0.24542338619815496
2213	24	-0.0836827075424651
2213	25	0.21418909287688592
2214	1	-0.8483396252623265
2214	2	-0.6864502221779354
2214	3	-0.7703957081937247
2214	4	-0.5932661101626167
2214	5	-0.8771860671003218
2214	6	-0.7418325902028222
2214	7	-0.7860248975063371
2214	8	-0.8012210693358703
2214	9	-10.647449371113883
2214	10	-3.9557177279048013
2214	11	-1.820687609377643
2214	12	-1.1875359686110087
2214	13	-0.9451736347769746
2214	14	-1.0081527064589508
2214	15	-1.0189793849344824
2214	16	-1.119634122154359
2214	17	-1.018321393109403
2214	18	-1.3936119524512733
2214	19	-1.5682289244967471
2214	20	-1.214355352279405
2214	21	-1.7684290879988431
2214	22	-1.4788652666178344
2214	23	-1.851660130475203
2214	24	-2.1287448983354373
2214	25	-9.83096628627732
2215	1	-0.6067883055285535
2215	2	-0.27779699924606405
2215	3	-0.2187245869020984
2215	4	-0.405549866071766
2215	5	-0.4764827778457672
2215	6	-0.4629591292104962
2215	7	-0.5636554187967953
2215	8	-0.5716994808637459
2215	9	0.26214171885708026
2215	10	0.1919278927528154
2215	11	0.23130461888926135
2215	12	0.22616376200786448
2215	13	0.11725910467129097
2215	14	-0.12588216833947286
2215	15	-0.599886667574296
2215	16	-0.2890023679047431
2215	17	-0.3152545153009143
2215	18	-0.2460768306454284
2215	19	-0.30258006659090597
2215	20	-0.12602262860833946
2215	21	-0.20769664013665334
2215	22	-0.28201669854504985
2215	23	0.10665783274638585
2215	24	0.31567733466462755
2215	25	0.4523022592399073
2216	1	-0.9732876544690541
2216	2	-0.951612015550257
2216	3	-1.0106551987279708
2216	4	-0.8641566510156906
2216	5	-1.760397353319636
2216	6	-2.381772194392205
2216	7	-1.6605997149642953
2216	8	-2.6941644730428784
2216	9	-1.186209472874875
2216	10	-1.697543412643956
2216	11	-1.1809474963832898
2216	12	-2.4477823095103375
2216	13	-2.1305339942443844
2216	14	-2.3483913822444578
2216	15	-1.9912658355242314
2216	16	-2.91779396620894
2216	17	-3.425865715308497
2216	18	-2.902540092009211
2216	19	-4.307531993846204
2216	20	-2.9909319538586843
2216	21	-3.2898625303618125
2216	22	-3.0780223023332987
2216	23	-2.7151163038653614
2216	24	-2.79902308910426
2216	25	-2.8592345652610334
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	191542.0
3	5.0
4	2948.0
5	27936.0
6	34550.0
7	28276.0
8	22407.0
9	18884.0
10	16776.0
11	15001.0
12	13663.0
13	12924.0
14	12952.0
15	13447.0
16	14060.0
17	15240.0
18	17084.0
19	20158.0
20	26041.0
21	34066.0
22	42713.0
23	51324.0
24	60676.0
25	70038.0
26	78768.0
27	88104.0
28	98465.0
29	115395.0
30	141400.0
31	173857.0
32	213215.0
33	263245.0
34	323644.0
35	401389.0
36	514802.0
37	734111.0
38	1684761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.824089761978115	18.569815895102927	12.929150272848332	25.676944070070622
2	17.059325241146837	19.36556085130817	37.94428501172103	25.63082889582396
3	20.511378455775002	22.745184408407717	28.089006859483995	28.654430276333287
4	13.350065332742906	15.454335639569921	35.04210918300015	36.15348984468702
5	14.22654119611185	37.25724771994051	33.820398138864164	14.695812945083475
6	34.84387674337652	35.39938650953892	16.11931257744839	13.637424169636173
7	30.607883578158567	30.653657001501045	19.61494895637489	19.1235104639655
8	27.096155383262726	36.014946667961276	18.137381386259285	18.751516562516706
9	26.062081758393624	15.383690989273452	18.63346884207887	39.92075841025405
10	15.463872541121177	29.26361992243322	31.675586530126914	23.596921006318684
11	36.883399490817474	22.240807281066893	21.052422622926663	19.82337060518897
12	23.55496271791387	24.55332919634207	29.116873936365067	22.774834149378993
13	29.442822683251713	20.745093643524434	24.968156213949484	24.84392745927437
14	22.131283529918232	20.209717973251866	26.469863656321824	31.189134840508075
15	24.764229581238556	28.48233234783911	22.247688421018005	24.505749649904327
16	24.7157717535263	27.602141956079603	24.15446655625066	23.527619734143432
17	23.334639652557225	27.40266789479744	25.694166831744415	23.568525620900918
18	23.096095195150845	26.793517964591107	26.85282362528692	23.25756321497113
19	24.3857177339329	26.259058278725895	26.45485834845654	22.90036563888467
20	23.97762291875408	25.674874004209514	26.990901555575103	23.3566015214613
21	24.575401034717217	25.309808180226806	26.592110911444735	23.522679873611242
22	24.224119744120603	25.258722104819014	26.557195624734053	23.95996252632633
23	23.52376539005175	25.26691345814503	26.95741005537612	24.2519110964271
24	23.755309715527968	25.549957808321054	26.80864643373046	23.886086042420516
25	23.864324142268057	25.378135078844732	26.805561670047233	23.95197910883998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	440.0
1	440.0
2	868.0
3	1296.0
4	1296.0
5	1296.0
6	3260.0
7	5224.0
8	5224.0
9	5224.0
10	7751.5
11	10279.0
12	10279.0
13	10279.0
14	14391.5
15	18504.0
16	18504.0
17	18504.0
18	33057.0
19	47610.0
20	47610.0
21	47610.0
22	73838.0
23	100066.0
24	100066.0
25	100066.0
26	141626.0
27	183186.0
28	183186.0
29	183186.0
30	233784.0
31	284382.0
32	284382.0
33	284382.0
34	339912.0
35	395442.0
36	395442.0
37	395442.0
38	453820.5
39	512199.0
40	512199.0
41	512199.0
42	563219.0
43	614239.0
44	614239.0
45	614239.0
46	663455.5
47	712672.0
48	712672.0
49	712672.0
50	730471.5
51	748271.0
52	748271.0
53	748271.0
54	709642.0
55	671013.0
56	671013.0
57	671013.0
58	599480.0
59	527947.0
60	527947.0
61	527947.0
62	446234.5
63	364522.0
64	364522.0
65	364522.0
66	289050.5
67	213579.0
68	213579.0
69	213579.0
70	160381.0
71	107183.0
72	107183.0
73	107183.0
74	75386.5
75	43590.0
76	43590.0
77	43590.0
78	32014.5
79	20439.0
80	20439.0
81	20439.0
82	14263.0
83	8087.0
84	8087.0
85	8087.0
86	5408.5
87	2730.0
88	2730.0
89	2730.0
90	1798.5
91	867.0
92	867.0
93	867.0
94	475.5
95	84.0
96	84.0
97	84.0
98	50.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027994945178353364
2	0.0013586308004820279
3	1.0726032635384431E-4
4	0.003468083885440966
5	0.03214234446403534
6	0.011190827382917757
7	0.019700146606989405
8	0.025921245535512377
9	0.03314344084333789
10	0.036468510960307066
11	0.038595840766324975
12	0.007686990055358842
13	0.002753015043082004
14	0.006346235975935788
15	0.007079181539353724
16	0.008491442503012675
17	0.00970705953502291
18	0.014462267336710008
19	0.016392953211079205
20	0.014712541431535645
21	0.011584115246215184
22	0.010851169682797248
23	0.008598702829366518
24	0.006203222207463996
25	0.10813628568573402
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5593867.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.89041890455195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18409037467724	31.6818996478862
2	13.50263572010858	11.852726761422641
3	5.445345030864275	7.169954234533603
4	2.722828768324469	4.7802438098850475
5	1.5020169252967852	3.29620760265015
6	0.9676323176480659	2.548187266029365
7	0.6744745853211668	2.0722080463153985
8	0.46931074729076966	1.6478596236000158
9	0.3442338552325767	1.3597711296558017
>10	1.9036903639344234	15.363028712676781
>50	0.16621562785931757	5.070781182183539
>100	0.10921305804767487	8.948735341370197
>500	0.006428980516065142	1.9227131869486147
>1k	0.0016789008701729754	1.235975743775371
>5k	8.189760342155294E-5	0.2414439165578519
>10k+	1.228464051323294E-4	0.8082637945094326
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	18702	0.3343304372449327	No Hit
GGTATCAACGCAGAGTACTTTTTTT	13472	0.2408351861064984	No Hit
TATCAACGCAGAGTACTTTTTTTTT	12798	0.2287862761127499	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	7509	0.13423629843183615	No Hit
GTGGTATCAACGCAGAGTACATGGG	5925	0.10591957227442125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.787672105897405E-5
2	0.0	0.0	0.0	0.0	3.57534421179481E-5
3	0.0	0.0	0.0	0.0	3.57534421179481E-5
4	0.0	0.0	0.0	1.787672105897405E-5	3.57534421179481E-5
5	0.0	0.0	0.0	1.787672105897405E-5	3.57534421179481E-5
6	3.57534421179481E-5	0.0	0.0	3.57534421179481E-5	3.57534421179481E-5
7	3.57534421179481E-5	0.0	0.0	3.57534421179481E-5	3.57534421179481E-5
8	3.57534421179481E-5	0.0	0.0	3.57534421179481E-5	3.57534421179481E-5
9	3.57534421179481E-5	0.0	0.0	3.57534421179481E-5	5.3630163176922156E-5
10	7.15068842358962E-5	0.0	0.0	3.57534421179481E-5	5.3630163176922156E-5
11	8.938360529487025E-5	0.0	0.0	3.57534421179481E-5	5.3630163176922156E-5
12	1.430137684717924E-4	0.0	0.0	3.57534421179481E-5	8.938360529487025E-5
13	1.6089048953076647E-4	1.787672105897405E-5	0.0	3.57534421179481E-5	8.938360529487025E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4725	0.0	13.212509	1
TCGATAC	60	4.092431E-4	12.667602	3
TGGTATC	1570	0.0	11.919664	2
CGTGCCG	100	1.9306262E-6	11.400843	10
CGAGTGG	430	0.0	11.268274	10
ACCGAGT	460	0.0	10.946951	8
AGGACCG	635	0.0	10.924998	5
TAGGCCG	70	0.0014881971	10.860859	5
CGATACT	155	3.6634447E-9	10.42059	4
GTGGTAT	1920	0.0	10.195011	1
GTCGGTT	205	3.6379788E-12	10.191865	12
CTACCGA	75	0.0026468064	10.134535	4
TTAGCGC	85	6.5914594E-4	10.060018	4
GGACCGA	515	0.0	9.777946	6
ACGTCGC	110	6.852433E-5	9.498834	11
CAGGACC	1705	0.0	9.3618145	4
CGGTTTG	255	0.0	9.311583	14
GTATCAA	9290	0.0	9.29758	1
ACCGGTC	125	2.753166E-5	9.121081	8
GACGGGT	125	2.753166E-5	9.121081	8
>>END_MODULE