FastQCFastQC Report
Thu 26 May 2016
SRR1364297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239114
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT107390.8666676351005638No Hit
TATCAACGCAGAGTACTTTTTTTTT66010.5327193462425572No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA57230.46186226610303815No Hit
GGTATCAACGCAGAGTACTTTTTTT47460.3830156063122521No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38440.3102216583784866No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN33590.2710807883697545No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25030.20199917037496146No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23600.19045866643424253No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA20730.16729695572804437No Hit
GTGGTATCAACGCAGAGTACATGGG17600.14203697157807918No Hit
GAACAAAAAAAAAAAAAAAAAAAAA14930.12048931736708648No Hit
GTATCAACGCAGATTACTTTTTTTT12780.10313821004362794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAAG508.761985E-515.1897322
TATAACG953.074092E-1014.9898662
CGCATAT1350.014.776778
GTACTAG653.395915E-614.6049191
ACCGAGT602.5690686E-514.2490278
ACCGTGC400.00528067914.2490278
CTCAACG802.013674E-613.0536763
AACTGGC1900.012.51029817
CGTTCAA550.00304896512.10087114
CGAGAAC550.003083396712.0827423
CAACCGT951.0400418E-611.9991826
GGTATCA23700.011.8564831
GTGTTAT1302.6411726E-911.6839361
CTACACT907.429042E-611.6150254
GTTTAAG1157.1377144E-811.5569371
GTTATCA3700.011.5457811
CGGCCCC1001.9083855E-611.40985516
GCCCCCG1001.9093168E-611.40939118
AATACCT1001.9205254E-611.4038435
GTACTAA1001.9450163E-611.3918371
Produced by FastQC (version 0.11.5)