##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364297_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1239114
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.466460713057877	30.0	25.0	31.0	2.0	34.0
2	25.364934945452962	30.0	25.0	31.0	2.0	34.0
3	24.926084282802066	30.0	22.0	31.0	2.0	34.0
4	28.481898356406273	35.0	27.0	37.0	2.0	37.0
5	29.235525544865123	35.0	28.0	37.0	2.0	37.0
6	29.30526489088171	35.0	29.0	37.0	2.0	37.0
7	28.91366492509971	35.0	28.0	37.0	2.0	37.0
8	28.427234298054902	35.0	27.0	37.0	2.0	37.0
9	29.305299593096358	35.0	27.0	39.0	2.0	39.0
10	28.61941112762829	35.0	25.0	39.0	2.0	39.0
11	29.272483403464086	35.0	27.0	39.0	2.0	39.0
12	28.791463900819455	35.0	25.0	39.0	2.0	39.0
13	28.41994037675307	35.0	23.0	39.0	2.0	39.0
14	28.6501936060766	35.0	19.0	40.0	2.0	41.0
15	29.34833276034328	36.0	26.0	40.0	2.0	41.0
16	29.717891977655	36.0	27.0	40.0	2.0	41.0
17	29.92980387599527	36.0	27.0	40.0	2.0	41.0
18	29.750366794338536	36.0	27.0	40.0	2.0	41.0
19	29.73952194874725	37.0	27.0	40.0	2.0	41.0
20	29.601537066000382	37.0	27.0	40.0	2.0	41.0
21	29.532949349293123	37.0	26.0	40.0	2.0	41.0
22	29.62048528222585	37.0	27.0	40.0	2.0	41.0
23	29.628175454397255	37.0	27.0	40.0	2.0	41.0
24	29.502893196267657	37.0	27.0	40.0	2.0	41.0
25	29.320069824083983	37.0	26.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.2009915254500463
1101	2	1.3647274224823036
1101	3	2.005322544487832
1101	4	1.5277839811460368
1101	5	1.162301799839895
1101	6	0.9058428474489233
1101	7	0.9512723388783897
1101	8	1.2660937421856922
1101	9	1.4239252667587508
1101	10	1.9792231936695046
1101	11	1.2675313327672555
1101	12	1.606558746580351
1101	13	1.5911662716602493
1101	14	2.2052243122869406
1101	15	1.4655807395286509
1101	16	0.9536201947546594
1101	17	0.30040102275705394
1101	18	-5.594046114978294
1101	19	-7.017951822651995
1101	20	-7.91802783697829
1101	21	-9.525026236421393
1101	22	-10.007818466440941
1101	23	-10.91462156464761
1101	24	-10.636351988500522
1101	25	-10.999766287645386
1102	1	2.3607847026902213
1102	2	2.5009591993423115
1102	3	3.2524530458800243
1102	4	2.6944803695496944
1102	5	2.0764351950484787
1102	6	2.0283680062237934
1102	7	2.348232311804587
1102	8	2.6192923414812874
1102	9	2.9465230749157456
1102	10	-0.6873794699385236
1102	11	1.4745233038453023
1102	12	2.4721300321400435
1102	13	3.0318906579401563
1102	14	3.4979952428069723
1102	15	3.232612308066301
1102	16	2.9004108551041057
1102	17	2.502389102989529
1102	18	2.7976890805569568
1102	19	2.5328733608459544
1102	20	2.9233893021964477
1102	21	2.9604930293436205
1102	22	2.869344296545769
1102	23	2.772845251708535
1102	24	3.066898083439593
1102	25	3.036553337212254
1103	1	0.2847266161956199
1103	2	0.7754193298348362
1103	3	-1.1215148506497954
1103	4	0.5160924718650719
1103	5	-2.5288610114299566
1103	6	-6.215583723090834
1103	7	-6.294898065237966
1103	8	-0.9594506575371327
1103	9	-6.8176304558885725
1103	10	-3.7892834482282645
1103	11	-7.960504700081021
1103	12	-1.5605797086071114
1103	13	0.5305193316511065
1103	14	1.411572816569894
1103	15	1.136994778185823
1103	16	0.7140548299294451
1103	17	1.1592183079089828
1103	18	1.3364903970301967
1103	19	-5.523841807466301
1103	20	-0.4428630795807962
1103	21	-5.848813223904255
1103	22	-1.6476890194655454
1103	23	-0.872212199645471
1103	24	-5.03293447040301
1103	25	-2.787151842070614
1104	1	1.7461463354122344
1104	2	1.6811176669358439
1104	3	2.6318888614265212
1104	4	2.1245832794648045
1104	5	1.6251235813317173
1104	6	1.523682055428285
1104	7	1.7365711824703354
1104	8	1.9458050689660986
1104	9	2.1936981806513103
1104	10	2.3300312003535524
1104	11	2.037604145546645
1104	12	2.321181076825514
1104	13	2.523338797738358
1104	14	3.0931748474575294
1104	15	1.972179028564419
1104	16	1.212752603707873
1104	17	1.148924868056472
1104	18	1.2242282545022682
1104	19	1.625167988493871
1104	20	-8.292664453502617
1104	21	-2.9446695108037133
1104	22	-0.540460200760311
1104	23	0.5575846384322105
1104	24	1.159517007038481
1104	25	1.5957355885199966
1105	1	1.1215074681938617
1105	2	1.1114535854343082
1105	3	1.8846555489988646
1105	4	1.2077042417377086
1105	5	1.2947226512841432
1105	6	1.1689792984287095
1105	7	1.3560022421840792
1105	8	1.3196666020149124
1105	9	1.5246620342300048
1105	10	2.020883438025578
1105	11	1.3997939384784885
1105	12	1.6000043932813384
1105	13	1.6642259631838279
1105	14	2.3713664046243537
1105	15	1.6632574044484478
1105	16	1.3647575088929926
1105	17	0.5343390808558439
1105	18	1.1196919780578654
1105	19	1.0344146703553925
1105	20	1.0251650275777457
1105	21	0.9646913874264449
1105	22	0.9782661847505274
1105	23	0.8945423018916401
1105	24	0.8345446688152585
1105	25	0.8860804811617093
1106	1	1.1710421407622427
1106	2	1.3657436773648506
1106	3	1.9873510432673918
1106	4	1.6334334438714073
1106	5	0.8302010305827459
1106	6	0.7054167062144323
1106	7	1.3698145222884968
1106	8	1.3766805618378584
1106	9	1.5298033348769238
1106	10	-1.9205250286637856
1106	11	0.12551179116263356
1106	12	1.0145487128818367
1106	13	1.9283446784261358
1106	14	0.7274156612354084
1106	15	1.5210601510152415
1106	16	1.088290592538435
1106	17	-5.728111265302367
1106	18	-1.5324530679074044
1106	19	0.18394934292377485
1106	20	0.724166276017197
1106	21	1.2010379996157532
1106	22	1.1432985306817507
1106	23	0.6137909683313154
1106	24	0.9852369852379113
1106	25	1.138792999604565
1107	1	0.8437610173006647
1107	2	1.1964327263135992
1107	3	1.6778010738387898
1107	4	1.193590334129098
1107	5	0.7907250351836517
1107	6	1.1049341571521012
1107	7	1.0517769331176225
1107	8	1.2002217755352902
1107	9	1.2448243227735318
1107	10	-1.8993055929803973
1107	11	0.30423471538522406
1107	12	0.7810195371557853
1107	13	1.394789541478115
1107	14	2.0288084498878796
1107	15	1.6400204967939906
1107	16	-5.265784675431341
1107	17	-1.6989017999644638
1107	18	-0.013308984670782564
1107	19	0.9102830363145316
1107	20	1.3389014844306928
1107	21	1.4526733248032357
1107	22	1.1745390495559178
1107	23	0.9068774537124185
1107	24	0.9168999920934269
1107	25	1.2373060500120907
1108	1	-0.7539528669901152
1108	2	-3.201298810583186
1108	3	-0.24840432300675985
1108	4	-0.9984225326592799
1108	5	-1.078680779130547
1108	6	-0.7426482358702664
1108	7	-0.43139992689669526
1108	8	-0.1321695693197249
1108	9	-0.7697984353584069
1108	10	-3.398238694249059
1108	11	-1.7465993702484965
1108	12	-6.096989599874373
1108	13	-2.1098096850073986
1108	14	-6.212290533229105
1108	15	-2.9233941675664816
1108	16	-7.358802037440352
1108	17	-3.5346760448123007
1108	18	-2.069788807277579
1108	19	-0.813763632633556
1108	20	-0.7951393467696164
1108	21	-0.33697574448633816
1108	22	-0.4354895920554043
1108	23	-0.4031335352928984
1108	24	-0.227904283347101
1108	25	-0.4360771235445142
1109	1	-9.196305458880172
1109	2	-6.799377894618342
1109	3	-5.770765612188647
1109	4	-5.5088411422065775
1109	5	-3.053725888028101
1109	6	-2.0016039680856608
1109	7	-1.6820094271805601
1109	8	-0.9979834380764672
1109	9	-5.7942126839956885
1109	10	-1.9587972934025295
1109	11	-1.640372579421431
1109	12	-6.5278172580800025
1109	13	-8.133698110301903
1109	14	-8.537391418237316
1109	15	-4.50529179195896
1109	16	-2.9546631076569305
1109	17	-2.3446563149393533
1109	18	-1.689600900805658
1109	19	-1.3707308941261687
1109	20	-1.2555462287700543
1109	21	-1.0908306325738586
1109	22	-1.3156580803669478
1109	23	-1.5881346927174569
1109	24	-1.4077345890655977
1109	25	-1.336078452155487
1110	1	-2.140109431440571
1110	2	-2.0420623336198496
1110	3	-1.4257920836845202
1110	4	-2.26446902527238
1110	5	-4.998375401823651
1110	6	-3.85117179851245
1110	7	-8.376478926049646
1110	8	-9.294185262957267
1110	9	-10.92038217306457
1110	10	-8.869138308912873
1110	11	-11.477630574552453
1110	12	-6.405585603126973
1110	13	-4.504364225109235
1110	14	-3.92143225907056
1110	15	-9.030321344561376
1110	16	-10.737963072468602
1110	17	-11.486559874602293
1110	18	-8.455415403600906
1110	19	-11.210782798733941
1110	20	-12.368539184538545
1110	21	-13.444177378164191
1110	22	-14.322064447435926
1110	23	-14.778355207050682
1110	24	-15.02896008156236
1110	25	-15.551155738807097
1111	1	-0.8242363780354189
1111	2	-0.1228359114765496
1111	3	-2.76508457064417
1111	4	-3.1889026034128243
1111	5	-1.2116174487452795
1111	6	-0.8823348135650129
1111	7	-0.22139307016268717
1111	8	0.24657157406551278
1111	9	-4.440945497396946
1111	10	-4.436525603849482
1111	11	-1.4399334323168453
1111	12	-5.678111719300794
1111	13	-1.309462376523932
1111	14	-0.2712539739183555
1111	15	-0.16349065870738144
1111	16	-0.14191111913486054
1111	17	-0.15859511547641247
1111	18	0.1788711484020844
1111	19	0.2599417861737692
1111	20	0.2025358229097094
1111	21	0.1848296633899622
1111	22	-0.1138491432871298
1111	23	-0.316161826773655
1111	24	8.631938716483489E-4
1111	25	0.14877048802647508
1112	1	-0.39806943006059825
1112	2	-0.06552646769730686
1112	3	-2.471214443646762
1112	4	-0.6946446558563579
1112	5	-0.6129319031757596
1112	6	-0.4759041572274043
1112	7	-0.018254440542140316
1112	8	-3.5803671680782934
1112	9	-0.9735729155651249
1112	10	-3.5247099400343664
1112	11	-1.1605929917449949
1112	12	-0.06944117683287843
1112	13	0.38052040837871814
1112	14	1.1976162732962123
1112	15	0.5527946791268832
1112	16	0.1018813475770095
1112	17	0.0619682702235167
1112	18	-0.04456350395393116
1112	19	0.42223567385247307
1112	20	0.2613275442751828
1112	21	0.20822103756827914
1112	22	-0.04713342801906961
1112	23	-0.3060580553209036
1112	24	0.05348368753139354
1112	25	-0.05882098521078305
1113	1	-1.6872139311026153
1113	2	-1.1443843466017078
1113	3	-3.620380433832647
1113	4	-2.2334097738172005
1113	5	-2.221503508073006
1113	6	-2.184028414483155
1113	7	-1.9392699687430834
1113	8	-8.894279568960012
1113	9	-9.875115291745406
1113	10	-4.171882883334991
1113	11	-9.319698106572663
1113	12	-8.398612768754354
1113	13	-3.6270720004783534
1113	14	-1.9359059999323982
1113	15	-7.561088288906827
1113	16	-3.7154261164983
1113	17	-3.00316535648885
1113	18	-2.1933054079427166
1113	19	-2.145455920537561
1113	20	-6.914525810515645
1113	21	-3.3205685602935695
1113	22	-2.727329710302236
1113	23	-2.7431896502173103
1113	24	-8.08108930672287
1113	25	-5.215799339408207
1114	1	-4.653107098525968
1114	2	-4.745843065283395
1114	3	-1.6578934224974766
1114	4	-1.4817438832367316
1114	5	-1.4846786491697905
1114	6	-1.5499805428995508
1114	7	-1.2561578199759822
1114	8	-0.7995242948075649
1114	9	-1.3298128768238549
1114	10	-4.161144329528504
1114	11	-1.909420677315076
1114	12	-5.408398461174148
1114	13	-2.086082792388087
1114	14	-6.586432229490072
1114	15	-2.9470133189801686
1114	16	-2.070724845010414
1114	17	-2.0005687014203595
1114	18	-1.5166164503558264
1114	19	-1.3254685750541668
1114	20	-1.311636182907673
1114	21	-1.056171083436027
1114	22	-0.9542298280086428
1114	23	-1.3468278776364357
1114	24	-1.1867512866912229
1114	25	-1.0290546760787116
1115	1	-0.17784048327637336
1115	2	0.14101167262251124
1115	3	0.9461668213994727
1115	4	-0.46149099511796265
1115	5	-0.49078732467555497
1115	6	-1.3427266953136652
1115	7	-2.9425864746967036
1115	8	-0.7548055066411301
1115	9	-0.6543984033953656
1115	10	0.2066228431939905
1115	11	-0.5782262839581058
1115	12	-0.05368484881113744
1115	13	0.3545228175281814
1115	14	0.8640887263764867
1115	15	0.29692403538642864
1115	16	-0.13240180532039147
1115	17	-0.7569668536789962
1115	18	-0.4068802117533359
1115	19	-0.10721358797322011
1115	20	-0.09630025689915911
1115	21	0.034647900510798024
1115	22	-0.4736807734622239
1115	23	-0.5660372174044035
1115	24	-0.5230956556012138
1115	25	-0.47563087188365216
1116	1	0.07885801349507204
1116	2	0.1302305390309968
1116	3	1.0665609406765526
1116	4	0.3736674794277022
1116	5	-0.6883088017662864
1116	6	-1.5937015901535077
1116	7	-5.092398271491575
1116	8	-1.6156533910849689
1116	9	-1.7073947767641897
1116	10	-0.8217890170757194
1116	11	-2.6263326020693043
1116	12	-1.024760331768018
1116	13	-7.323191858294717
1116	14	-2.538885066443058
1116	15	-2.1947572774751514
1116	16	-1.6070545394845226
1116	17	-1.1976554373815596
1116	18	-0.9457470421249887
1116	19	-0.683262097321375
1116	20	-0.701931363275758
1116	21	-0.5190962038564031
1116	22	-0.7325555046290368
1116	23	-0.8039147544401786
1116	24	-0.6944310465930954
1116	25	-0.7362347010206456
1201	1	2.556154552534199
1201	2	2.698309878425569
1201	3	3.409471190072175
1201	4	2.955609676452962
1201	5	2.477451783093027
1201	6	2.258453798025908
1201	7	2.513072949254788
1201	8	2.575225256039822
1201	9	3.0432435549587638
1201	10	3.332682409333753
1201	11	2.8097896006213894
1201	12	3.169726150733158
1201	13	3.2477328108011037
1201	14	3.7856503483561497
1201	15	3.4013097066111797
1201	16	2.9342468811431033
1201	17	2.756288637774727
1201	18	2.6441946737262043
1201	19	2.6592736899996865
1201	20	2.8967830482755232
1201	21	2.81369677876312
1201	22	2.114936569580422
1201	23	1.6032923793533733
1201	24	2.0766280796103267
1201	25	2.1095761742893053
1202	1	2.6279752744093976
1202	2	2.8152926827648272
1202	3	3.4101542290384117
1202	4	3.1616269195756246
1202	5	2.6766845508040653
1202	6	2.3982085230666144
1202	7	2.6687238069742634
1202	8	2.9577900157689996
1202	9	3.3257322724344505
1202	10	-0.3495285527194092
1202	11	1.8432873998326968
1202	12	2.8246774609917438
1202	13	3.433552426607161
1202	14	4.124043019409946
1202	15	3.6617172807085296
1202	16	3.2561583997388865
1202	17	2.9293889743416592
1202	18	3.1601423954507624
1202	19	3.2768791741216106
1202	20	3.291945852986224
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2211	12	-5.374881348995707
2211	13	-1.1145674656096034
2211	14	-5.680718949634915
2211	15	-1.9095890727458524
2211	16	-1.0206959265604425
2211	17	-0.6171792076189675
2211	18	-0.04191380746096485
2211	19	0.1968143479724631
2211	20	0.45083018367768446
2211	21	0.5446411478109852
2211	22	0.21980580455101517
2211	23	0.21562025775220306
2211	24	0.30947674157341964
2211	25	0.3158104130655559
2212	1	0.7601698377994488
2212	2	0.7483378272752397
2212	3	1.5311333199713424
2212	4	0.9473878924896937
2212	5	0.27757912749961733
2212	6	0.423687594089845
2212	7	0.575873719225914
2212	8	0.9781217016153434
2212	9	0.702881184345209
2212	10	1.5008296761527617
2212	11	0.7444512916055217
2212	12	0.9425448119045292
2212	13	1.194277683466538
2212	14	-4.259794579616518
2212	15	-1.1281640283589311
2212	16	-0.5322243403987699
2212	17	-0.03185170087873246
2212	18	0.19498727948406014
2212	19	0.6795867958451325
2212	20	1.0113152691659089
2212	21	0.905737556528436
2212	22	0.8610847457211577
2212	23	0.7514783612338576
2212	24	0.7284233011443249
2212	25	0.7292100443586449
2213	1	0.6544043986636083
2213	2	0.8349310788147122
2213	3	1.4635562060548608
2213	4	0.8397381732862037
2213	5	0.2311009978566645
2213	6	0.013781691289356246
2213	7	0.12035450265284453
2213	8	-5.4689624065170115
2213	9	-1.803081419727043
2213	10	-0.13264210870385185
2213	11	-0.1510984808605329
2213	12	0.6029562418642556
2213	13	-7.551209949339466
2213	14	-2.162030672947484
2213	15	-0.84455861987243
2213	16	-0.24045480845524736
2213	17	-0.23383783392301183
2213	18	0.095861439664219
2213	19	0.440524310445614
2213	20	0.6297202889291249
2213	21	0.6377422847491871
2213	22	0.38032561399442244
2213	23	0.5042265227409075
2213	24	0.46736804824058
2213	25	0.5249840802594825
2214	1	-1.0615606648742713
2214	2	-0.6938465198657973
2214	3	-6.921442107098791
2214	4	-2.885906576873108
2214	5	-1.7944519128904233
2214	6	-1.3279864985370864
2214	7	-0.8923324561506192
2214	8	-6.472327664979357
2214	9	-2.1798863453183763
2214	10	-0.3846194674773251
2214	11	-0.7893055506210018
2214	12	-0.43962257134562677
2214	13	-6.412495888538025
2214	14	-1.835181701923755
2214	15	-1.1709202797292342
2214	16	-0.9775195083840238
2214	17	-0.8997879032711396
2214	18	-0.7828439994781142
2214	19	-0.41781808187736047
2214	20	-0.3875080350953155
2214	21	-0.20318623045117334
2214	22	-0.23501625353191002
2214	23	-0.3989944166450812
2214	24	-0.18145022217963103
2214	25	-0.1399510698698414
2215	1	-0.29393505348262394
2215	2	-0.02330316627985951
2215	3	-2.991355199844108
2215	4	-1.189657782817747
2215	5	-0.7279820768592735
2215	6	-1.1838431798170639
2215	7	-0.5460599394060637
2215	8	-0.1548276996471678
2215	9	0.03868010099306929
2215	10	0.6996555096914427
2215	11	-0.15770356088663462
2215	12	-0.38161762474396355
2215	13	0.1942524984181624
2215	14	0.20975620307059017
2215	15	0.0017972409872584194
2215	16	-0.5142711045891879
2215	17	-0.5889691683887825
2215	18	-0.5527950782414024
2215	19	-0.4061969357000308
2215	20	-0.2825690603513138
2215	21	-0.01644656987877724
2215	22	-0.2610842202745829
2215	23	-0.2199230286845193
2215	24	-0.6076699348924173
2215	25	-0.47340120071607217
2216	1	0.5358010202736736
2216	2	0.40695617444834653
2216	3	1.2278345498114511
2216	4	0.032453199311095915
2216	5	-0.16811654514284058
2216	6	-0.34278040073557037
2216	7	-0.8972323942566618
2216	8	-5.9095429924618585
2216	9	-1.6937101787986997
2216	10	-0.3160605763993516
2216	11	-1.2156256715976106
2216	12	0.07250597990506336
2216	13	0.706504592103677
2216	14	1.7195757440550672
2216	15	1.1120388181153587
2216	16	0.5419246589609052
2216	17	0.43747407139541394
2216	18	0.5401718798354764
2216	19	0.8111595116040498
2216	20	0.8747222557609291
2216	21	0.6434765971276839
2216	22	0.44764998161523906
2216	23	0.24870400109150737
2216	24	0.19546894386167324
2216	25	0.1996361790685519
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	132482.0
3	1.0
4	785.0
5	6097.0
6	9978.0
7	8505.0
8	7313.0
9	5897.0
10	5335.0
11	5095.0
12	4805.0
13	4822.0
14	4675.0
15	4579.0
16	4331.0
17	4741.0
18	5673.0
19	7575.0
20	10564.0
21	13306.0
22	15964.0
23	18013.0
24	20159.0
25	22383.0
26	23808.0
27	25833.0
28	27814.0
29	31904.0
30	35630.0
31	40411.0
32	49536.0
33	62971.0
34	78806.0
35	97712.0
36	122272.0
37	142749.0
38	176590.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.10393353953782	18.928195494809803	13.124078962284011	20.843792003368367
2	14.23996540899091	22.380269521612497	41.30726807951074	22.072496989885853
3	16.124758576848482	30.70983467828467	29.736367943054503	23.429038801812343
4	10.38959356230023	18.380517163609213	37.02130864371491	34.20858063037565
5	11.695985525897	41.409524303162485	32.265862837789136	14.628627333151382
6	28.236143532893227	39.21504032532223	18.262465882931913	14.286350258852632
7	27.266663482686297	35.90663706104581	20.19944129239382	16.627258163874075
8	26.06708230193512	35.594044899617764	19.666207428009887	18.672665370437226
9	27.676732026884494	18.5448116701299	19.333165500878692	34.44529080210692
10	13.652150941072172	33.56531266156571	30.80190078264661	21.980635614715506
11	32.10892877957804	27.28367719625442	21.777742161091034	18.829651863076506
12	22.77033289486998	30.871435798846502	28.10191237478498	18.256318931498534
13	26.74325898309859	25.60020203017664	26.264408612507577	21.392130374217196
14	20.744198621332753	25.055852536352717	28.78607345337042	25.413875388944113
15	23.094237913702255	31.483379064176965	24.230629143093026	21.191753879027754
16	21.025230081833858	32.50730514881458	25.296536428611898	21.17092834073966
17	20.69438857561878	31.619794857765836	26.00302865241155	21.68278791420383
18	19.962039660056657	29.634156212059892	29.65787130716309	20.745932820720355
19	21.536592433349465	29.23161474867136	29.95506178195575	19.27673103602342
20	22.254149614843723	28.140901179718508	29.723699641364277	19.881249564073492
21	23.0859199207431	27.64821008259165	29.370461263513008	19.89540873315224
22	22.474573938945078	27.922327582369107	29.09575092375622	20.507347554929602
23	21.6644340932177	28.469803600985838	29.27644385448747	20.589318451308987
24	21.47719399351175	28.45611041681506	29.500981007945192	20.565714581728
25	21.32083351544024	28.127018351519744	29.873221220229567	20.678926912810454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9761.0
1	9761.0
2	8967.5
3	8174.0
4	8174.0
5	8174.0
6	11291.0
7	14408.0
8	14408.0
9	14408.0
10	14271.5
11	14135.0
12	14135.0
13	14135.0
14	14194.5
15	14254.0
16	14254.0
17	14254.0
18	19740.0
19	25226.0
20	25226.0
21	25226.0
22	33795.0
23	42364.0
24	42364.0
25	42364.0
26	56762.0
27	71160.0
28	71160.0
29	71160.0
30	82790.0
31	94420.0
32	94420.0
33	94420.0
34	101209.0
35	107998.0
36	107998.0
37	107998.0
38	114082.5
39	120167.0
40	120167.0
41	120167.0
42	129434.0
43	138701.0
44	138701.0
45	138701.0
46	143929.0
47	149157.0
48	149157.0
49	149157.0
50	142893.0
51	136629.0
52	136629.0
53	136629.0
54	122938.5
55	109248.0
56	109248.0
57	109248.0
58	94187.0
59	79126.0
60	79126.0
61	79126.0
62	64567.0
63	50008.0
64	50008.0
65	50008.0
66	38711.5
67	27415.0
68	27415.0
69	27415.0
70	20894.0
71	14373.0
72	14373.0
73	14373.0
74	10512.5
75	6652.0
76	6652.0
77	6652.0
78	5090.0
79	3528.0
80	3528.0
81	3528.0
82	2416.0
83	1304.0
84	1304.0
85	1304.0
86	958.5
87	613.0
88	613.0
89	613.0
90	432.0
91	251.0
92	251.0
93	251.0
94	140.0
95	29.0
96	29.0
97	29.0
98	21.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3303973645685546
2	0.3317693125894792
3	0.3429063024063968
4	0.34403614195304066
5	0.3516222074805062
6	0.29553374427211704
7	0.3036847295729045
8	0.3015057533043772
9	0.30303910697482234
10	0.4112615949783474
11	0.3016671589538977
12	0.301747861778658
13	0.29496882449879513
14	0.30005310245869227
15	0.29246703693122666
16	0.297309206416843
17	0.288996815466535
18	0.2916600086836239
19	0.293758282127391
20	0.492932853635743
21	0.29359687647787047
22	0.29609866404543894
23	0.2920635228074253
24	0.2945653103749937
25	0.2885933013427336
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1239114.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.47855183067375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.58287803245638	50.90345646871687
2	7.646305590459869	9.095823667506757
3	2.647919082733133	4.724831772173184
4	1.2176649877374526	2.896998003421552
5	0.6693529054904662	1.9906070741113386
6	0.44100473669356244	1.5738193853400413
7	0.30008535568186456	1.2494049667084968
8	0.20370193593035132	0.969271692339359
9	0.16101175011646257	0.8619071152184559
>10	0.9759325566551309	11.066453456927512
>50	0.0990419219468743	4.101781659074282
>100	0.050520953788757796	5.489081793453019
>500	0.0024982856234844215	0.971064475520643
>1k	0.0016655237489896146	2.201591303040043
>5k	2.7758729149826903E-4	1.0173764002649328
>10k+	1.3879364574913451E-4	0.8865307661834722
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10739	0.8666676351005638	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6601	0.5327193462425572	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5723	0.46186226610303815	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4746	0.3830156063122521	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3844	0.3102216583784866	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3359	0.2710807883697545	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2503	0.20199917037496146	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2360	0.19045866643424253	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2073	0.16729695572804437	No Hit
GTGGTATCAACGCAGAGTACATGGG	1760	0.14203697157807918	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	1493	0.12048931736708648	No Hit
GTATCAACGCAGATTACTTTTTTTT	1278	0.10313821004362794	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6140564952054452E-4	0.0
5	0.0	0.0	0.0	1.6140564952054452E-4	0.0
6	0.0	0.0	0.0	1.6140564952054452E-4	0.0
7	0.0	0.0	8.070282476027226E-5	1.6140564952054452E-4	0.0
8	0.0	0.0	8.070282476027226E-5	1.6140564952054452E-4	0.0
9	0.0	0.0	8.070282476027226E-5	2.421084742808168E-4	0.0
10	0.0	0.0	8.070282476027226E-5	2.421084742808168E-4	0.0
11	0.0	0.0	8.070282476027226E-5	2.421084742808168E-4	0.0
12	0.0	0.0	8.070282476027226E-5	2.421084742808168E-4	0.0
13	0.0	0.0	8.070282476027226E-5	2.421084742808168E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTAAG	50	8.761985E-5	15.189732	2
TATAACG	95	3.074092E-10	14.989866	2
CGCATAT	135	0.0	14.77677	8
GTACTAG	65	3.395915E-6	14.604919	1
ACCGAGT	60	2.5690686E-5	14.249027	8
ACCGTGC	40	0.005280679	14.249027	8
CTCAACG	80	2.013674E-6	13.053676	3
AACTGGC	190	0.0	12.510298	17
CGTTCAA	55	0.003048965	12.100871	14
CGAGAAC	55	0.0030833967	12.082742	3
CAACCGT	95	1.0400418E-6	11.999182	6
GGTATCA	2370	0.0	11.856483	1
GTGTTAT	130	2.6411726E-9	11.683936	1
CTACACT	90	7.429042E-6	11.615025	4
GTTTAAG	115	7.1377144E-8	11.556937	1
GTTATCA	370	0.0	11.545781	1
CGGCCCC	100	1.9083855E-6	11.409855	16
GCCCCCG	100	1.9093168E-6	11.409391	18
AATACCT	100	1.9205254E-6	11.403843	5
GTACTAA	100	1.9450163E-6	11.391837	1
>>END_MODULE