FastQCFastQC Report
Thu 26 May 2016
SRR1364297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239114
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT254652.055097432520333No Hit
TATCAACGCAGAGTACTTTTTTTTT186341.5038164365829132No Hit
GGTATCAACGCAGAGTACTTTTTTT180241.4545877134791472No Hit
GTACTTTTTTTTTTTTTTTTTTTTT106260.8575482159026531No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80370.6486086025983081No Hit
GAGTACTTTTTTTTTTTTTTTTTTT69010.5569301936706388No Hit
GTGGTATCAACGCAGAGTACTTTTT37810.3051373804185894No Hit
GTGGTATCAACGCAGAGTACATGGG29240.23597505959903606No Hit
ATCAACGCAGAGTACTTTTTTTTTT29110.23492592287715253No Hit
GCAGAGTACTTTTTTTTTTTTTTTT24330.19634997264174242No Hit
GTATAACGCAGAGTACTTTTTTTTT20550.1658443048823595No Hit
GGTATCAACGCAGAGTACATGGGAT16600.13396668910205195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA1100.015.5460510
TATAACG2750.015.5454222
TATCACG953.0559022E-1014.9999682
CGCCAGT1151.8189894E-1214.86533312
TAACGCA2950.014.1711794
ATAACGC3000.013.93386653
GGTATCA43050.013.4411671
TTAGGCA1403.6379788E-1212.8943914
GACATAT1700.012.8549911
GTAATAC604.0896639E-412.6671513
TAGTCGT604.0994244E-412.66357315
TAATACC1001.4392572E-712.351474
TAGGACA1551.8189894E-1212.2595234
GCGCCAG1405.2750693E-1112.21327311
TTCGTGC550.00305123112.09967619
CTGTGCG550.003066034512.091869
ACGAAAT550.003074388712.08746812
TATAAGC2600.012.0581533
TAGACAG1501.4551915E-1112.0371955
CTATAAG2150.011.9302062
Produced by FastQC (version 0.11.5)