##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364297_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1239114 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.477712300885955 31.0 30.0 34.0 26.0 34.0 2 29.442616256454208 31.0 30.0 34.0 25.0 34.0 3 29.469876056601734 31.0 30.0 34.0 25.0 34.0 4 32.78935352195197 35.0 33.0 37.0 26.0 37.0 5 32.42248816493075 35.0 32.0 37.0 25.0 37.0 6 32.28416513734814 35.0 32.0 37.0 23.0 37.0 7 31.996129492524496 35.0 32.0 37.0 19.0 37.0 8 31.851255816656096 35.0 32.0 37.0 17.0 37.0 9 32.49781376047724 37.0 32.0 39.0 11.0 39.0 10 32.644092472524726 37.0 32.0 39.0 17.0 39.0 11 32.57386406739009 37.0 32.0 39.0 13.0 39.0 12 32.51411734513531 37.0 32.0 39.0 14.0 39.0 13 32.533264090309686 37.0 32.0 39.0 13.0 39.0 14 33.40689799324356 38.0 32.0 41.0 13.0 41.0 15 33.442111863799454 38.0 32.0 41.0 13.0 41.0 16 33.40594166477015 38.0 32.0 40.0 11.0 41.0 17 33.47415895551176 38.0 32.0 41.0 11.0 41.0 18 33.45272186417069 38.0 32.0 41.0 11.0 41.0 19 33.40492077403693 38.0 32.0 41.0 10.0 41.0 20 33.313004291776224 39.0 32.0 41.0 10.0 41.0 21 33.2607314581225 38.0 32.0 41.0 8.0 41.0 22 33.22242666937828 38.0 32.0 41.0 2.0 41.0 23 32.940994129676525 38.0 32.0 41.0 2.0 41.0 24 32.864778381972926 38.0 32.0 41.0 2.0 41.0 25 32.71723505666145 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3012219576663675 1101 2 0.5656653857155547 1101 3 -1.6332650808078881 1101 4 -0.35706143239131904 1101 5 0.6382259406533848 1101 6 0.8425900897543954 1101 7 1.0655831946755683 1101 8 0.8316111012033431 1101 9 1.70290343419898 1101 10 1.388889929945691 1101 11 1.4403444011838857 1101 12 1.396850790562489 1101 13 1.2443524737339757 1101 14 1.4463795076071406 1101 15 1.3154689265078048 1101 16 1.3826001686230924 1101 17 1.372205944211366 1101 18 1.196892226826705 1101 19 1.3327628487497094 1101 20 1.5113553036077505 1101 21 1.2968434537356117 1101 22 1.538021143759039 1101 23 1.686481064826431 1101 24 1.742926722977593 1101 25 1.8893061286226924 1102 1 0.7936571001077937 1102 2 0.7401510645667848 1102 3 0.7681774704519739 1102 4 0.7954647801542265 1102 5 0.8814048020136411 1102 6 0.799182337244595 1102 7 1.0097526004704989 1102 8 1.0345962106131097 1102 9 1.7964559788525136 1102 10 1.1207417697887578 1102 11 1.2061393090397488 1102 12 1.2397553675748867 1102 13 1.1779005803917855 1102 14 1.1956565601294997 1102 15 1.2306631850788108 1102 16 1.2774105506960183 1102 17 1.1397203221016312 1102 18 1.2214268452198667 1102 19 1.2819239864978016 1102 20 1.544792257724147 1102 21 1.6138652951593357 1102 22 1.4843553390227413 1102 23 1.772078147502846 1102 24 1.684741863230414 1102 25 1.7131339100706526 1103 1 -2.8327070086744683 1103 2 -2.4307667135959257 1103 3 -2.2955603319615605 1103 4 -2.320549423899127 1103 5 -5.113355288060848 1103 6 -10.584680761086716 1103 7 -11.687501859948696 1103 8 -11.649067684530955 1103 9 -12.643234534456568 1103 10 -9.650607615279664 1103 11 -14.11299718844856 1103 12 -12.116912165192595 1103 13 -12.828126037773238 1103 14 -12.71447826289723 1103 15 -12.197645320031182 1103 16 -7.470481937322919 1103 17 -5.443649098765622 1103 18 -4.701547922376417 1103 19 -4.382498035558903 1103 20 -9.52565060849215 1103 21 -5.329101966229889 1103 22 -12.990448344792721 1103 23 -12.606256340631571 1103 24 -13.78524052605303 1103 25 -14.841892361654043 1104 1 0.5000410544469247 1104 2 0.7360279809296024 1104 3 0.6614808344819743 1104 4 0.7863136009236484 1104 5 0.8206920749310527 1104 6 0.8848193449935096 1104 7 0.9074545739998996 1104 8 0.9650563488587096 1104 9 1.5812934085381158 1104 10 1.212384286043779 1104 11 1.0870445120540424 1104 12 1.2048035544337097 1104 13 1.028618104037733 1104 14 1.0471850750451068 1104 15 1.0228515743787554 1104 16 0.8348571901855095 1104 17 0.7612174230466664 1104 18 0.8927647143390445 1104 19 1.1271516070604761 1104 20 1.4557451146457794 1104 21 1.235182650674183 1104 22 0.9276943001896854 1104 23 1.607206337418603 1104 24 1.7619944379894292 1104 25 1.8198690090487943 1105 1 0.33738521793949516 1105 2 0.48104707647873113 1105 3 0.09335051564493924 1105 4 0.25779928053653123 1105 5 0.39905138738930646 1105 6 0.6226973680305434 1105 7 0.7573158826951314 1105 8 0.42952256579842896 1105 9 0.6614646311121675 1105 10 0.4031253667141854 1105 11 0.8574683363647893 1105 12 1.0903781290542227 1105 13 0.8525283320115662 1105 14 0.7705458916921373 1105 15 0.5972382987654825 1105 16 0.7369401092295647 1105 17 0.8537158551210737 1105 18 1.008553087724593 1105 19 0.9273614286483891 1105 20 1.1070020045952447 1105 21 0.9216885476041057 1105 22 0.9069166090939689 1105 23 1.2426896409231034 1105 24 1.2677019421068323 1105 25 1.6002903348764832 1106 1 0.02591092444273002 1106 2 0.20700326760346144 1106 3 0.029379343246802136 1106 4 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0.5606370365212499 1107 16 0.4913128838746488 1107 17 0.24165424048759832 1107 18 0.20616766007441356 1107 19 0.4968788418117924 1107 20 0.5243395250341294 1107 21 0.5490690087969696 1107 22 0.49767899849479136 1107 23 0.541389957248235 1107 24 0.7858967494846922 1107 25 0.3909893064377812 1108 1 -0.14559918080744083 1108 2 -0.16227250624719503 1108 3 0.018570473669402787 1108 4 -0.09668310731330365 1108 5 -0.11733819936875278 1108 6 0.003017457357799458 1108 7 -0.41875547572013616 1108 8 -0.040898637017861006 1108 9 0.41084998929997596 1108 10 0.06960955351566867 1108 11 0.31778025803802024 1108 12 0.40685921421493987 1108 13 0.06251819998505681 1108 14 0.12030137901160032 1108 15 -0.05201317192918253 1108 16 -0.6112263041364443 1108 17 -0.6042041845799275 1108 18 -0.5560344457472794 1108 19 -0.5057976958618084 1108 20 0.11602829617015686 1108 21 -0.019881192788560043 1108 22 0.27018387747894934 1108 23 -0.18248792801085756 1108 24 0.24107487305396802 1108 25 -0.052022455256164335 1109 1 0.26054441853254673 1109 2 0.2811943303454072 1109 3 0.17658652022393184 1109 4 0.2666350168310814 1109 5 0.2329079258139899 1109 6 0.41004816002437394 1109 7 0.6268961083162949 1109 8 0.4193007772691004 1109 9 1.2172103055298322 1109 10 0.5829876440053212 1109 11 0.5360401251462648 1109 12 0.6493225407195524 1109 13 0.7168210343721668 1109 14 0.1866772502099181 1109 15 -0.2408956619096685 1109 16 0.34872041856354485 1109 17 0.2868131788324746 1109 18 0.22489574545517144 1109 19 0.45109577662137923 1109 20 0.8258149467981042 1109 21 0.6200069790478508 1109 22 0.19932496625519747 1109 23 0.5689373905108468 1109 24 0.4042216574832622 1109 25 0.21270328111368997 1110 1 0.21811998893242546 1110 2 0.3181408589207848 1110 3 0.2365700957617456 1110 4 0.4034599790954303 1110 5 0.37164015868728484 1110 6 0.5862850224608707 1110 7 0.6389557100972567 1110 8 0.5445478798252026 1110 9 -3.031570444436305 1110 10 -0.9610440921216146 1110 11 -0.11540088187705067 1110 12 0.27761828512048226 1110 13 -0.11246426320509073 1110 14 0.34269505010530565 1110 15 0.4049405034720692 1110 16 -0.015576779088590342 1110 17 0.1934743755420385 1110 18 0.34099145089846417 1110 19 0.24507066912918418 1110 20 0.6173709153294737 1110 21 0.20776878495077966 1110 22 0.5361052318042709 1110 23 0.5869921398529812 1110 24 0.8517841179815022 1110 25 0.7147232662358576 1111 1 0.15519928089918977 1111 2 0.12006345384462591 1111 3 0.11048300559296464 1111 4 0.028648549929492617 1111 5 0.14255437846511398 1111 6 0.038760916132929424 1111 7 0.20196627006396994 1111 8 0.3734031189022815 1111 9 1.030136789590145 1111 10 0.42999601494405937 1111 11 0.6527267457008676 1111 12 0.6705438640743253 1111 13 0.4004443857528628 1111 14 0.25490403624446145 1111 15 0.5206324342955355 1111 16 0.3388328227727584 1111 17 0.5138364788339302 1111 18 0.599261139112258 1111 19 0.6515106882345449 1111 20 0.7360350535312108 1111 21 0.6195261468980178 1111 22 0.8039304366903224 1111 23 1.00754695189692 1111 24 0.7858935670945684 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11 -3.246696313327014 1113 12 -13.238179007809624 1113 13 -5.080406447089349 1113 14 -4.1752830042964995 1113 15 -3.5333632438168934 1113 16 -3.187086095307823 1113 17 -3.509213760524201 1113 18 -3.509854631641595 1113 19 -4.368167341481019 1113 20 -14.600522118424085 1113 21 -16.3457477966693 1113 22 -11.470283507083781 1113 23 -17.104473685633195 1113 24 -18.60489140348971 1113 25 -15.19710373360385 1114 1 -0.10537027218441608 1114 2 -0.06676096343457161 1114 3 -0.14747167386146032 1114 4 -0.691060777534048 1114 5 -0.004835661234587008 1114 6 0.21983575595840676 1114 7 0.14765040197702106 1114 8 0.1230601685465551 1114 9 0.8842767605032407 1114 10 -0.016696332054877416 1114 11 -0.10617620622711854 1114 12 0.1806073442834375 1114 13 -0.05098146228770162 1114 14 -0.16320354600466658 1114 15 0.03470813886689683 1114 16 1.5116717746366248E-4 1114 17 -0.22171047293683444 1114 18 -0.15465183600665 1114 19 -0.6546918981417775 1114 20 -0.7403868638462541 1114 21 -0.8349548090392531 1114 22 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-0.6067855867647545 2215 6 -0.7139741185126702 2215 7 -0.6881264835014669 2215 8 -0.8442922887501361 2215 9 0.006459474362522144 2215 10 -0.12812116737995893 2215 11 -0.049408279978216285 2215 12 -0.08046835528636365 2215 13 -0.060683053262415854 2215 14 -0.2713673401646517 2215 15 -0.7672239222128994 2215 16 -0.3334477094394259 2215 17 -0.5503779303477785 2215 18 -0.32049781120829834 2215 19 -0.32472407492505795 2215 20 -0.23813043835659187 2215 21 -0.3471602793001338 2215 22 -0.3588498290172595 2215 23 0.1378487745891661 2215 24 0.19484588020510074 2215 25 0.41735243872668093 2216 1 -0.8142740642541355 2216 2 -0.8421805017106792 2216 3 -0.8855847560384227 2216 4 -0.76002962507863 2216 5 -1.5356790645167173 2216 6 -1.8658682648098974 2216 7 -1.0222819156049532 2216 8 -1.9199792046957107 2216 9 -0.8382909991778185 2216 10 -1.3792067625645075 2216 11 -0.7678645580520751 2216 12 -1.9239874883648334 2216 13 -1.8532692207325532 2216 14 -2.092728069291745 2216 15 -1.6791868452169147 2216 16 -2.7925521118657137 2216 17 -3.2054079727050464 2216 18 -2.7369755990254703 2216 19 -4.389293375410997 2216 20 -3.0011882360377413 2216 21 -3.030638895944712 2216 22 -2.788026361421519 2216 23 -2.3708150477724246 2216 24 -2.517137349221013 2216 25 -2.512418372870396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47627.0 3 2.0 4 576.0 5 6683.0 6 9648.0 7 7743.0 8 6000.0 9 4847.0 10 4177.0 11 3566.0 12 3299.0 13 2949.0 14 2934.0 15 2844.0 16 3003.0 17 3171.0 18 3634.0 19 4796.0 20 6959.0 21 9264.0 22 11193.0 23 12534.0 24 14337.0 25 15949.0 26 17685.0 27 19293.0 28 21019.0 29 24247.0 30 29182.0 31 35952.0 32 44523.0 33 55019.0 34 68314.0 35 85838.0 36 112185.0 37 167504.0 38 370618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.44159210663343 19.243633099245912 13.639097416588697 21.675677377531965 2 16.02056652363286 21.516325142179692 39.51461583841834 22.94849249576911 3 18.522861533097895 28.647531458011866 29.90423787357398 22.92536913531626 4 11.675970845382224 17.817535868234426 36.37079728081779 34.135696005565556 5 11.887772030680726 39.935964762858916 33.248916398171296 14.92734680828906 6 28.98709923195878 38.4993171890789 17.632583156039246 14.881000422923076 7 26.44608353552916 34.794632003603205 20.977917471817694 17.781366989049946 8 27.207010706406486 34.483137187790355 19.037271016660597 19.27258108914256 9 27.42797079839412 15.609522571665918 19.89587462329428 37.06663200664568 10 16.356368914841894 31.697761028346207 29.808202323660698 22.1376677331512 11 33.0076010479193 24.47359816573756 22.28548131224039 20.23331947410275 12 23.90770792794 29.161686264096794 28.600195476597285 18.330410331365922 13 28.98162757195834 22.78842855816994 26.747559691223767 21.482384178647955 14 22.432489144997014 22.40851936145142 29.60728294029345 25.551708553258113 15 24.08111052822727 28.84887615511884 26.061095193898552 21.00891812275534 16 21.026366997548852 29.576654283133884 27.45609199595484 21.940886723362425 17 20.781029939346492 28.662515486216762 28.68245083756724 21.874003736869504 18 19.783380913984352 25.638989175502786 33.37514296391538 21.202486946597478 19 21.44742991314258 25.625157898730112 33.728379476756906 19.199032711370403 20 21.689281191457628 24.57809134002662 33.78174959017816 19.950877878337597 21 22.440439005570703 24.03642667932222 33.643103773904706 19.880030541202373 22 21.79700338015619 24.336963154037626 33.60051202748032 20.26552143832587 23 21.058022047910097 24.52145994968641 34.01324747039819 20.407270532005302 24 21.081307932254823 24.938198869299587 33.813753223222534 20.166739975223052 25 20.862457303693937 24.57618766016164 34.111319004824644 20.45003603131978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1564.0 1 1564.0 2 2081.0 3 2598.0 4 2598.0 5 2598.0 6 7766.5 7 12935.0 8 12935.0 9 12935.0 10 12107.5 11 11280.0 12 11280.0 13 11280.0 14 9835.5 15 8391.0 16 8391.0 17 8391.0 18 14574.0 19 20757.0 20 20757.0 21 20757.0 22 31628.0 23 42499.0 24 42499.0 25 42499.0 26 61433.5 27 80368.0 28 80368.0 29 80368.0 30 90250.0 31 100132.0 32 100132.0 33 100132.0 34 106496.5 35 112861.0 36 112861.0 37 112861.0 38 114124.5 39 115388.0 40 115388.0 41 115388.0 42 123674.0 43 131960.0 44 131960.0 45 131960.0 46 140563.0 47 149166.0 48 149166.0 49 149166.0 50 144218.5 51 139271.0 52 139271.0 53 139271.0 54 126557.0 55 113843.0 56 113843.0 57 113843.0 58 98168.0 59 82493.0 60 82493.0 61 82493.0 62 68586.5 63 54680.0 64 54680.0 65 54680.0 66 42206.5 67 29733.0 68 29733.0 69 29733.0 70 22613.5 71 15494.0 72 15494.0 73 15494.0 74 11192.5 75 6891.0 76 6891.0 77 6891.0 78 5480.0 79 4069.0 80 4069.0 81 4069.0 82 2872.0 83 1675.0 84 1675.0 85 1675.0 86 1175.5 87 676.0 88 676.0 89 676.0 90 519.5 91 363.0 92 363.0 93 363.0 94 192.5 95 22.0 96 22.0 97 22.0 98 13.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03252323837838972 2 0.0010491367218835395 3 1.6140564952054452E-4 4 0.005245683609417697 5 0.03397588922407462 6 0.009522933321712126 7 0.017431810148218808 8 0.02606701239756794 9 0.03349167227551299 10 0.037526813513526595 11 0.037849624812567685 12 0.007343957053184776 13 0.002340381918047896 14 0.004357952537054702 15 0.0051649807846574245 16 0.008312390950308043 17 0.008796607898869676 18 0.013315966085444922 19 0.015091428230170912 20 0.012993154786403832 21 0.010652772868355937 22 0.009522933321712126 23 0.006859740104623141 24 0.004761466660856063 25 0.10507507783787448 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1239114.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.66565495115493 #Duplication Level Percentage of deduplicated Percentage of total 1 74.77928049957131 31.15727698790767 2 12.634182146628023 10.528229478228903 3 4.711313593269505 5.888998996315593 4 2.318859164349725 3.864671432884765 5 1.2828285237449928 2.6724945315929163 6 0.8220362477896086 2.0550407194646354 7 0.5629157146920528 1.6417976354459292 8 0.4152116248991542 1.384005143580522 9 0.3103811869558283 1.1639011895128306 >10 1.852971979369503 14.922357512504943 >50 0.1847388578785209 5.377831950246191 >100 0.11514891563448595 8.5778336664734 >500 0.006819309280843172 1.9067624669769319 >1k 0.002143211488264997 1.7403428885835945 >5k 3.8967481604818124E-4 1.2126710546535 >10k+ 7.793496320963625E-4 5.90578434562776 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 25465 2.055097432520333 No Hit TATCAACGCAGAGTACTTTTTTTTT 18634 1.5038164365829132 No Hit GGTATCAACGCAGAGTACTTTTTTT 18024 1.4545877134791472 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 10626 0.8575482159026531 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 8037 0.6486086025983081 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 6901 0.5569301936706388 No Hit GTGGTATCAACGCAGAGTACTTTTT 3781 0.3051373804185894 No Hit GTGGTATCAACGCAGAGTACATGGG 2924 0.23597505959903606 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2911 0.23492592287715253 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2433 0.19634997264174242 No Hit GTATAACGCAGAGTACTTTTTTTTT 2055 0.1658443048823595 No Hit GGTATCAACGCAGAGTACATGGGAT 1660 0.13396668910205195 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.421084742808168E-4 0.0 7 0.0 0.0 0.0 2.421084742808168E-4 0.0 8 0.0 0.0 0.0 2.421084742808168E-4 0.0 9 0.0 0.0 0.0 2.421084742808168E-4 0.0 10 0.0 0.0 0.0 2.421084742808168E-4 0.0 11 0.0 0.0 0.0 2.421084742808168E-4 0.0 12 0.0 0.0 0.0 2.421084742808168E-4 0.0 13 0.0 0.0 0.0 2.421084742808168E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGCCA 110 0.0 15.54605 10 TATAACG 275 0.0 15.545422 2 TATCACG 95 3.0559022E-10 14.999968 2 CGCCAGT 115 1.8189894E-12 14.865333 12 TAACGCA 295 0.0 14.171179 4 ATAACGC 300 0.0 13.9338665 3 GGTATCA 4305 0.0 13.441167 1 TTAGGCA 140 3.6379788E-12 12.894391 4 GACATAT 170 0.0 12.854991 1 GTAATAC 60 4.0896639E-4 12.667151 3 TAGTCGT 60 4.0994244E-4 12.663573 15 TAATACC 100 1.4392572E-7 12.35147 4 TAGGACA 155 1.8189894E-12 12.259523 4 GCGCCAG 140 5.2750693E-11 12.213273 11 TTCGTGC 55 0.003051231 12.099676 19 CTGTGCG 55 0.0030660345 12.09186 9 ACGAAAT 55 0.0030743887 12.087468 12 TATAAGC 260 0.0 12.058153 3 TAGACAG 150 1.4551915E-11 12.037195 5 CTATAAG 215 0.0 11.930206 2 >>END_MODULE