FastQCFastQC Report
Thu 26 May 2016
SRR1364296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348107
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49270.36547544074765576No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN36670.2720110495680239No Hit
TATCAACGCAGAGTACTTTTTTTTT28840.2139296064778241No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28680.21274275706601925No Hit
GGTATCAACGCAGAGTACTTTTTTT22480.16675234235858133No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16520.1225422017688507No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14670.10881925544485713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG607.7334334E-817.4066312
ATCACGC801.2970668E-714.2417913
ATAACGC1251.03682396E-1012.9125573
CTAAGCT604.0727382E-412.6735254
GGTATCA12150.012.5807561
GTACTAA1052.7403257E-711.754691
TTAGACT1157.016388E-811.571484
TAACGCA1657.2759576E-1211.5213864
ACTAGCC752.0845416E-411.3934323
AGACCGT855.3142554E-511.1779396
GTATCAC1201.2852615E-711.0765361
ATAAGAC1408.418283E-910.8508883
AACGCAT2150.010.6060456
GTATCAA27800.010.5870281
GTTATCA2450.010.4629661
TATAACG1553.6925485E-910.4133522
TAGGGTG1303.8097642E-710.2363085
AGGACCG750.002635307410.138825
GGACCGA856.583737E-410.0601456
GTATTAG1901.0004442E-109.9938671
Produced by FastQC (version 0.11.5)