##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364296_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1348107
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.939782969749434	30.0	25.0	31.0	2.0	34.0
2	24.65838023242962	30.0	22.0	31.0	2.0	34.0
3	24.252319734264418	30.0	16.0	31.0	2.0	34.0
4	27.85548179780982	33.0	26.0	35.0	2.0	37.0
5	28.417963114203843	35.0	27.0	37.0	2.0	37.0
6	28.540164096766798	35.0	28.0	37.0	2.0	37.0
7	28.15893174651567	35.0	27.0	37.0	2.0	37.0
8	27.779039052538113	35.0	26.0	37.0	2.0	37.0
9	28.764734550002338	35.0	26.0	39.0	2.0	39.0
10	27.968300735772456	35.0	21.0	39.0	2.0	39.0
11	28.65064345782642	35.0	26.0	39.0	2.0	39.0
12	28.14434610902547	35.0	23.0	39.0	2.0	39.0
13	27.781032959549947	35.0	19.0	39.0	2.0	39.0
14	27.962399127072256	34.0	17.0	40.0	2.0	41.0
15	28.663551928741562	35.0	23.0	40.0	2.0	41.0
16	28.961780481816355	36.0	25.0	40.0	2.0	41.0
17	29.097722955225365	36.0	25.0	40.0	2.0	41.0
18	28.90347947158497	36.0	25.0	40.0	2.0	41.0
19	28.87733614616644	36.0	24.0	40.0	2.0	41.0
20	28.787355899791336	36.0	23.0	40.0	2.0	41.0
21	28.662603932773884	36.0	22.0	40.0	2.0	41.0
22	28.715657585043324	36.0	23.0	40.0	2.0	41.0
23	28.702170525039925	36.0	24.0	40.0	2.0	41.0
24	28.587086188262504	36.0	23.0	40.0	2.0	41.0
25	28.37262324133025	36.0	21.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8542728843732519
1101	2	1.1023923672614266
1101	3	1.7782914990296028
1101	4	1.2936077527934486
1101	5	0.6036205275369859
1101	6	0.5381500524436937
1101	7	0.8781954872155886
1101	8	1.076246632359247
1101	9	0.9780116104398999
1101	10	1.4157796822778224
1101	11	0.8852596504722676
1101	12	1.063506438794498
1101	13	1.201188388964006
1101	14	1.8152188344976317
1101	15	0.929567070109421
1101	16	0.378287980273857
1101	17	-0.3844615047888702
1101	18	-6.141468741728058
1101	19	-7.477741311573606
1101	20	-8.515875958714389
1101	21	-10.064572344095513
1101	22	-10.427958529800595
1101	23	-11.480509595951183
1101	24	-11.597288529359513
1101	25	-11.835035269136792
1102	1	2.2401744502103895
1102	2	2.5139231589118687
1102	3	3.091128531608927
1102	4	2.58286872958319
1102	5	2.125231912010772
1102	6	2.108199729718404
1102	7	2.467360885400776
1102	8	2.779410166171651
1102	9	3.043795649992923
1102	10	-0.6378033354101191
1102	11	1.6296635821748673
1102	12	2.626393620641643
1102	13	3.171677019829996
1102	14	3.6093118407397426
1102	15	3.3338645673659606
1102	16	2.9300833240618402
1102	17	2.656849692067656
1102	18	2.879770948172471
1102	19	2.4691122643641847
1102	20	2.925354999313626
1102	21	2.8946592511231977
1102	22	2.9366443469974044
1102	23	2.939469872348578
1102	24	3.189736380076809
1102	25	3.286679953820954
1103	1	0.9789857593635958
1103	2	0.8837870731844362
1103	3	-1.1375488574322858
1103	4	1.0871013476188978
1103	5	-2.2071513864535746
1103	6	-6.6799064009068765
1103	7	-6.571264412854745
1103	8	-2.1714187618100347
1103	9	-5.962538775171527
1103	10	-4.122421665024522
1103	11	-8.498188939413613
1103	12	-2.9432869897525364
1103	13	-1.3642354583688316
1103	14	-0.3500635338923921
1103	15	-0.03166038168406615
1103	16	-0.4041678177202286
1103	17	-0.3799000285292813
1103	18	-0.3505770584945296
1103	19	-5.310385544883765
1103	20	-1.8598120817848063
1103	21	-5.639424055462207
1103	22	-2.7892583472602333
1103	23	-1.7074613345566405
1103	24	-6.753273129295277
1103	25	-3.6540479249973927
1104	1	1.6679601442982914
1104	2	1.6895596138967335
1104	3	2.4949462165936183
1104	4	1.9775889606437183
1104	5	1.6135353683224807
1104	6	1.4127252764165092
1104	7	1.6524332218988782
1104	8	1.8361512726912572
1104	9	2.0738783866885804
1104	10	2.513893472708048
1104	11	1.7779654519073276
1104	12	2.1336010407145167
1104	13	2.3712066332036663
1104	14	2.7060302660001305
1104	15	1.7022863944313826
1104	16	1.070992033059749
1104	17	0.9401052673796286
1104	18	0.7902902244705565
1104	19	1.190385477148773
1104	20	-8.796257280809737
1104	21	-3.403183683502487
1104	22	-0.9659275320796681
1104	23	0.004495737428779734
1104	24	0.7361817814920215
1104	25	1.2814393375628654
1105	1	1.4579773560022495
1105	2	1.5414957011900938
1105	3	2.10498379919472
1105	4	1.6284109407630858
1105	5	1.6032599067765894
1105	6	1.4874120116066436
1105	7	1.7891926412540293
1105	8	2.0351153369779986
1105	9	1.740597925053116
1105	10	2.375234823313388
1105	11	2.1363064428977268
1105	12	2.200235765884372
1105	13	2.067525364772308
1105	14	2.954537514447736
1105	15	2.3662651251523314
1105	16	1.7812608091760715
1105	17	0.9350457517630311
1105	18	1.6601836814450088
1105	19	1.5099544723112288
1105	20	1.3708227464038956
1105	21	1.2747396298138831
1105	22	1.5458222196199856
1105	23	1.3587671370300143
1105	24	1.353807326649477
1105	25	1.3324734162123555
1106	1	0.9293580576366622
1106	2	1.2060875587209168
1106	3	1.8114999818257722
1106	4	1.538404894408604
1106	5	0.869343740079568
1106	6	0.649478009630915
1106	7	1.033833921356873
1106	8	0.9848218100529884
1106	9	1.2697142026112544
1106	10	-2.2080160751198186
1106	11	-0.08129934968590646
1106	12	0.5337656790890541
1106	13	1.4278165467319184
1106	14	0.6207157783111867
1106	15	1.3260637515307323
1106	16	0.9273325181728644
1106	17	-5.502036389057217
1106	18	-1.582821990933759
1106	19	-0.19328959209384422
1106	20	0.4948427528632706
1106	21	0.7652325893881695
1106	22	0.8235755847807482
1106	23	0.4192327130959299
1106	24	0.7654584418396375
1106	25	0.7958557320135178
1107	1	0.9242922910671858
1107	2	1.288684259331621
1107	3	1.7838606154477752
1107	4	1.186883305856739
1107	5	0.6297549669968987
1107	6	0.9690941083877505
1107	7	0.9853707412068822
1107	8	1.0489267627634327
1107	9	1.2105895670744609
1107	10	-1.4944932209196082
1107	11	0.3755020142405776
1107	12	0.8644861194911613
1107	13	1.599349975804838
1107	14	2.2122733326724138
1107	15	1.9044868044631187
1107	16	-4.87664203467974
1107	17	-1.4838637731180278
1107	18	0.30467424657295084
1107	19	1.095689385088221
1107	20	1.5592060936671928
1107	21	1.716393839877746
1107	22	1.3625989865823946
1107	23	1.209794204022426
1107	24	1.4309920540507868
1107	25	1.5955541885753064
1108	1	-0.5992740345824714
1108	2	-3.523349000618307
1108	3	-0.5865421299549709
1108	4	-0.9786691201123539
1108	5	-0.9295372068786101
1108	6	-0.6627046484110082
1108	7	-0.3536309207114492
1108	8	-0.31185385322551795
1108	9	-0.6656838508656229
1108	10	-3.440265041691429
1108	11	-1.9474375847406904
1108	12	-6.319155855817481
1108	13	-2.179377469589273
1108	14	-5.974286442429026
1108	15	-2.949717633074652
1108	16	-7.974271642726016
1108	17	-3.8097602917228777
1108	18	-2.267353455663084
1108	19	-1.0346228181406474
1108	20	-0.8583117665590017
1108	21	-0.47599344105120167
1108	22	-0.8552283249949184
1108	23	-0.6499625515676613
1108	24	-0.4103473549969543
1108	25	-0.7243273188061998
1109	1	-8.30237845918628
1109	2	-6.311712130214396
1109	3	-5.172712551162565
1109	4	-5.1812907675914985
1109	5	-2.475668458709986
1109	6	-1.3432721189413037
1109	7	-0.9911239923742841
1109	8	-0.4092393547312163
1109	9	-5.492223883415569
1109	10	-1.3047613295148537
1109	11	-0.8129713579572382
1109	12	-5.527707189059026
1109	13	-7.325526108457861
1109	14	-7.15360372320232
1109	15	-3.6221409977223047
1109	16	-1.9786929809453717
1109	17	-1.5732749621458524
1109	18	-0.903379220251427
1109	19	-0.5916169965722489
1109	20	-0.3554698244357688
1109	21	-0.3747845565399608
1109	22	-0.46718937097083213
1109	23	-0.8137568267312041
1109	24	-0.5194082078620212
1109	25	-0.4618483685949073
1110	1	-3.031641908560065
1110	2	-2.7796688330960713
1110	3	-2.127327001035969
1110	4	-2.953341982332674
1110	5	-5.873574310976458
1110	6	-4.603862680457421
1110	7	-9.225957087190992
1110	8	-9.764305574849601
1110	9	-11.672869741270954
1110	10	-9.8058810168746
1110	11	-12.534581810913009
1110	12	-7.528509324700181
1110	13	-5.696625726548994
1110	14	-5.400613867921223
1110	15	-9.992610593203754
1110	16	-11.82672098477293
1110	17	-12.232682325958503
1110	18	-9.547315252633233
1110	19	-12.514309665784975
1110	20	-13.49392322054555
1110	21	-14.489687258693387
1110	22	-15.477736745453225
1110	23	-16.098196580819156
1110	24	-16.32622540815671
1110	25	-16.51274894526883
1111	1	-0.8688991573149387
1111	2	-0.0728911546802351
1111	3	-2.4755041036627077
1111	4	-3.6652015891306995
1111	5	-1.4021032105180602
1111	6	-0.6473862008891693
1111	7	-0.3301306712224985
1111	8	-0.038866611311721755
1111	9	-4.623609616700996
1111	10	-4.68830317108549
1111	11	-1.824156981234097
1111	12	-6.24551159008282
1111	13	-1.636869294312735
1111	14	-0.2563831849777465
1111	15	-0.2893005884768165
1111	16	-0.3592985644761022
1111	17	-0.456531139058697
1111	18	-0.05560868177141742
1111	19	0.2148726573435269
1111	20	7.067478267437366E-4
1111	21	0.36289716620098744
1111	22	-0.11015665619443382
1111	23	-0.23470200636361227
1111	24	-0.014181523802875518
1111	25	0.27394800997760527
1112	1	-1.1059045452290484
1112	2	-0.6162791256769466
1112	3	-3.115804478494425
1112	4	-1.4139773044165231
1112	5	-1.3568075417794674
1112	6	-1.0645685141302934
1112	7	-0.7572053323179873
1112	8	-4.433079496539513
1112	9	-1.8556860248035427
1112	10	-3.5361528664949162
1112	11	-1.720180979974046
1112	12	-0.4257131390815907
1112	13	-0.05696760219948516
1112	14	0.540135919382891
1112	15	-0.17537810973914603
1112	16	-0.3330858168012334
1112	17	-0.7035462618140684
1112	18	-0.6096170971235146
1112	19	-0.42777949041378704
1112	20	-0.3374991714492168
1112	21	-0.417548836421858
1112	22	-0.45109898713063856
1112	23	-0.6155227982524067
1112	24	-0.5696569580816586
1112	25	-0.6059563992304611
1113	1	-1.3734570582542496
1113	2	-0.9943899645018064
1113	3	-3.167965932237667
1113	4	-1.9935812321614819
1113	5	-2.203886276728703
1113	6	-2.0844706806064863
1113	7	-1.593553082924
1113	8	-8.047138374808608
1113	9	-9.941367116323942
1113	10	-4.338400080930761
1113	11	-8.744068886484467
1113	12	-7.812149872328881
1113	13	-3.32703039229332
1113	14	-1.4361249523247963
1113	15	-6.991637010372354
1113	16	-3.843823434568197
1113	17	-2.7097276651053868
1113	18	-2.1487760927094435
1113	19	-1.688244663991533
1113	20	-6.692638463871383
1113	21	-3.120701228908274
1113	22	-2.5450453246278677
1113	23	-2.4327624979667917
1113	24	-8.028814020498384
1113	25	-5.828490708245663
1114	1	-4.861314750854621
1114	2	-4.967434244358127
1114	3	-1.6739231810665913
1114	4	-1.212850757575513
1114	5	-1.3364436120578986
1114	6	-1.2482673298286109
1114	7	-1.1475141308295456
1114	8	-0.8896943904788337
1114	9	-1.2686261653796151
1114	10	-3.9810884477998094
1114	11	-2.062540162710281
1114	12	-5.400999043014728
1114	13	-2.205485948202071
1114	14	-6.763245292700486
1114	15	-3.025133230079195
1114	16	-1.995209509950584
1114	17	-1.9830222948639822
1114	18	-1.442099677904988
1114	19	-1.1582898502705454
1114	20	-1.0456597604700342
1114	21	-1.0378969632310628
1114	22	-1.0497744236860953
1114	23	-1.4043426306454947
1114	24	-1.1495175432172289
1114	25	-1.0105554832727073
1115	1	0.16685803471051486
1115	2	0.25776080060643736
1115	3	0.9441706119646689
1115	4	-0.13429180734423696
1115	5	-0.2818720793284264
1115	6	-0.9175307609957422
1115	7	-3.068149373735647
1115	8	-0.7907360289467711
1115	9	-0.5823832646914759
1115	10	0.09450016117722271
1115	11	-0.7032542988907373
1115	12	-0.09959434027895497
1115	13	-7.458096357737531E-4
1115	14	0.5558655043895868
1115	15	-0.39762918299769723
1115	16	-0.5779982694411494
1115	17	-1.003668501435687
1115	18	-0.5606329199195592
1115	19	-0.2791908831110099
1115	20	-0.4734425914599427
1115	21	-0.2609387532953633
1115	22	-0.7379648413521238
1115	23	-0.8948949286839465
1115	24	-1.0055228797701794
1115	25	-0.6317838469523842
1116	1	0.12812316111738298
1116	2	0.4763295000826915
1116	3	1.0986263711644497
1116	4	0.4816000282958548
1116	5	-0.3580041425030096
1116	6	-1.4044186263213057
1116	7	-5.059624843474285
1116	8	-1.4602263093689345
1116	9	-1.4219397134609544
1116	10	-0.6343658738290721
1116	11	-2.6869950809444028
1116	12	-1.0930571041563582
1116	13	-6.648093786976347
1116	14	-2.1972329649665525
1116	15	-1.7492127679481086
1116	16	-1.240365542766746
1116	17	-0.8287088883810867
1116	18	-0.7200718725941826
1116	19	-0.22849378092776718
1116	20	-0.33997068175574796
1116	21	-0.1907886057441175
1116	22	-0.4909460496696987
1116	23	-0.43015978370763364
1116	24	-0.21639535531898346
1116	25	-0.35572625388611456
1201	1	2.2952526634762336
1201	2	2.4968428915382646
1201	3	3.1675042896543637
1201	4	2.842930831730424
1201	5	2.151021021582487
1201	6	2.1683322322947483
1201	7	2.316013677189222
1201	8	2.404461921589153
1201	9	2.878743433076007
1201	10	3.264856425019442
1201	11	2.746538090471187
1201	12	3.017868333303838
1201	13	3.056934334051448
1201	14	3.599989334937259
1201	15	3.196343402611106
1201	16	2.8262289767591504
1201	17	2.5902986469674047
1201	18	2.865016463682551
1201	19	2.7499683980178986
1201	20	2.8957391453285553
1201	21	2.7894256301073845
1201	22	1.9443940540976605
1201	23	1.3801896198648613
1201	24	2.0001911703318562
1201	25	1.9395577291848092
1202	1	2.7313865815072838
1202	2	2.945246571769683
1202	3	3.5787341550817935
1202	4	3.2525635712001773
1202	5	2.6720004585313113
1202	6	2.591888728936862
1202	7	2.8395326097774323
1202	8	3.1496817649967284
1202	9	3.5044999979609415
1202	10	-0.4975692367201745
1202	11	1.973808242692762
1202	12	3.042457100605855
1202	13	3.562193519486705
1202	14	4.30583991454364
1202	15	3.7759990247777324
1202	16	3.4191409622634374
1202	17	2.854225127751562
1202	18	3.0983737793256267
1202	19	3.2809966551634595
1202	20	3.390717240953041
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2211	13	-0.6172736245396919
2211	14	-5.155374805687934
2211	15	-1.2607116921307266
2211	16	-0.2649803597913234
2211	17	0.17497877053556365
2211	18	0.5433314092853472
2211	19	0.8490539415051046
2211	20	1.0020318806063528
2211	21	1.0966119610115648
2211	22	0.984068037557261
2211	23	0.9374731250153019
2211	24	1.0615380665008693
2211	25	1.126762590423244
2212	1	0.8832588946584572
2212	2	1.1529253750951014
2212	3	1.669892099464203
2212	4	1.319109296945662
2212	5	0.7563360457203601
2212	6	0.7697255333548689
2212	7	0.8571507087297618
2212	8	1.065710102135899
2212	9	1.018756488726769
2212	10	1.7736006943177962
2212	11	0.970732021353335
2212	12	1.3327102116080773
2212	13	1.5321813900357206
2212	14	-3.933382601111841
2212	15	-0.9764281913765878
2212	16	-0.33879428123743693
2212	17	0.0417752846429309
2212	18	0.42917895374207404
2212	19	1.0574457328886346
2212	20	1.217068626211411
2212	21	1.1009872552090947
2212	22	1.1078507178006873
2212	23	1.0289976423024818
2212	24	0.9920841810336363
2212	25	1.2019821752623727
2213	1	0.7886003232276693
2213	2	1.0592937031569143
2213	3	1.657308314858696
2213	4	1.2451426727303776
2213	5	0.5169063627406736
2213	6	0.18463170855757483
2213	7	0.5936055631566752
2213	8	-4.9511984495169905
2213	9	-1.4750297610580034
2213	10	0.4927235173917026
2213	11	0.3255207546216212
2213	12	0.8034090616978169
2213	13	-7.22654027521676
2213	14	-2.013505968659647
2213	15	-0.48093483752595034
2213	16	0.013796390730224317
2213	17	0.362469248428436
2213	18	0.46905972791880757
2213	19	0.8848932671664471
2213	20	0.8639586874686032
2213	21	0.975356883182954
2213	22	0.5205107442642927
2213	23	0.564246122506745
2213	24	0.729449206574273
2213	25	0.8561582590524068
2214	1	-0.9921041691451222
2214	2	-0.5533131400351827
2214	3	-6.738138346959438
2214	4	-2.7523568237093343
2214	5	-1.7585749409499982
2214	6	-1.180282670817828
2214	7	-0.848136355661719
2214	8	-5.9998396824170825
2214	9	-2.013460636453356
2214	10	-0.21266373200056776
2214	11	-0.6149580351115951
2214	12	-0.3441398682173542
2214	13	-5.939865710469672
2214	14	-1.6540896219395513
2214	15	-0.8901828951670723
2214	16	-0.7036159551841727
2214	17	-0.6613499219194736
2214	18	-0.7608366847330856
2214	19	-0.41084961110727036
2214	20	-0.07390966111504582
2214	21	-0.11270889178479493
2214	22	-0.2550804970156584
2214	23	-0.26126793182241315
2214	24	-0.20736820015944346
2214	25	0.06901742054380833
2215	1	0.07545243905872923
2215	2	0.3984050133837691
2215	3	-2.359659469118821
2215	4	-0.5769283572863202
2215	5	-0.35528114998689375
2215	6	-0.8985345033106285
2215	7	-0.2608598789481569
2215	8	0.33051987410945216
2215	9	0.6278305818736136
2215	10	1.012501141307311
2215	11	0.41179738069582683
2215	12	0.0853000729795319
2215	13	0.8464817587505742
2215	14	0.9052970289373903
2215	15	0.5336552329338708
2215	16	0.29662170739214133
2215	17	0.16953518741756213
2215	18	0.4436070337470177
2215	19	0.233722330824655
2215	20	0.44580449042547343
2215	21	0.6072751823724616
2215	22	0.20091558277689714
2215	23	0.24844642389557592
2215	24	-0.012983897323419313
2215	25	0.2519477756084285
2216	1	-0.03391861082775094
2216	2	-0.32721933465574793
2216	3	0.711319238618799
2216	4	-0.756369972307489
2216	5	-0.8970385286455418
2216	6	-1.1823830643954913
2216	7	-1.7015630758555638
2216	8	-6.078906400665048
2216	9	-2.4035445178379895
2216	10	-1.207563901338574
2216	11	-1.8145632991839058
2216	12	-0.560909658872955
2216	13	0.10823300131055547
2216	14	0.7948423440260548
2216	15	0.24757067749422035
2216	16	-0.40425207305824884
2216	17	-0.4989350831843886
2216	18	-0.18748089654690148
2216	19	-0.036670993099992444
2216	20	-0.08103112349120067
2216	21	-0.22963713277756526
2216	22	-0.3222310086202924
2216	23	-0.7951800104174858
2216	24	-0.8128771292065871
2216	25	-0.793494943910499
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	171220.0
3	8.0
4	1184.0
5	7121.0
6	9523.0
7	8479.0
8	7486.0
9	6553.0
10	5824.0
11	5366.0
12	4923.0
13	4733.0
14	4803.0
15	4836.0
16	5056.0
17	5521.0
18	6175.0
19	7748.0
20	10724.0
21	13444.0
22	16536.0
23	19295.0
24	21913.0
25	24902.0
26	27033.0
27	29791.0
28	32558.0
29	37086.0
30	42142.0
31	46829.0
32	57343.0
33	70478.0
34	87112.0
35	105972.0
36	127561.0
37	144480.0
38	166349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.95487915974492	17.388239882345236	11.797926751573394	23.858954206336445
2	15.232832055017806	20.60220383829588	39.628227886257825	24.536736220428487
3	17.26221714050912	26.55162839377421	30.108182426552744	26.077972039163928
4	10.984959299663902	16.114578797116174	37.251061707441444	35.64940019577848
5	12.463801130670287	38.53860046423886	34.11038998744858	14.887208417642267
6	31.21159567795835	36.54302139668042	17.973092515588647	14.27229040977258
7	27.948981421612356	32.60879432075305	21.52351856164633	17.918705695988262
8	26.372148913136485	34.49518416970175	20.67953616243987	18.453130754721894
9	26.879990714258078	16.44484060964467	19.73235039389998	36.94281828219727
10	13.889046149078688	30.111841791614026	33.431637505661364	22.56747455364592
11	34.343976208846684	24.153199075317573	22.333521077298183	19.16930363853756
12	22.653974272455425	26.621513132000057	30.192402271714357	20.532110323830157
13	27.82284764516556	22.778899564462392	26.280163620482615	23.118089169889426
14	21.190963534051924	21.952186758986652	28.45788867882273	28.398961028138697
15	23.44703676700344	29.53695299596768	23.90435520109512	23.111655035933758
16	22.884562731758418	29.169717098488775	25.314417680005235	22.631302489747572
17	21.86565626281015	28.595490529695738	26.502642192197438	23.03621101529667
18	21.544440840279616	27.90330356425449	28.46143659364761	22.090819001818286
19	22.822777021804036	27.335059879816292	28.314072742612396	21.528090355767276
20	23.044891697410236	26.82499014143408	28.34533382334195	21.784784337813733
21	23.46587425823309	26.436810685014674	28.21266167096202	21.884653385790216
22	23.134829170157268	26.585054825360878	27.959222847347565	22.320893157134293
23	22.68528265839576	26.794946096005724	27.909703462934544	22.610067782663975
24	22.35186720015951	27.095494524952795	28.32829153826581	22.224346736621882
25	22.521369746836385	26.56975576369412	28.484663626421856	22.424210863047634
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5757.0
1	5757.0
2	4715.0
3	3673.0
4	3673.0
5	3673.0
6	5988.0
7	8303.0
8	8303.0
9	8303.0
10	8588.5
11	8874.0
12	8874.0
13	8874.0
14	10188.0
15	11502.0
16	11502.0
17	11502.0
18	16134.0
19	20766.0
20	20766.0
21	20766.0
22	28129.5
23	35493.0
24	35493.0
25	35493.0
26	47299.5
27	59106.0
28	59106.0
29	59106.0
30	72419.0
31	85732.0
32	85732.0
33	85732.0
34	97055.0
35	108378.0
36	108378.0
37	108378.0
38	119614.5
39	130851.0
40	130851.0
41	130851.0
42	141338.5
43	151826.0
44	151826.0
45	151826.0
46	158844.5
47	165863.0
48	165863.0
49	165863.0
50	163814.0
51	161765.0
52	161765.0
53	161765.0
54	150361.0
55	138957.0
56	138957.0
57	138957.0
58	122898.0
59	106839.0
60	106839.0
61	106839.0
62	88798.0
63	70757.0
64	70757.0
65	70757.0
66	54916.5
67	39076.0
68	39076.0
69	39076.0
70	29621.5
71	20167.0
72	20167.0
73	20167.0
74	14193.0
75	8219.0
76	8219.0
77	8219.0
78	6024.0
79	3829.0
80	3829.0
81	3829.0
82	2626.5
83	1424.0
84	1424.0
85	1424.0
86	1030.5
87	637.0
88	637.0
89	637.0
90	437.5
91	238.0
92	238.0
93	238.0
94	147.0
95	56.0
96	56.0
97	56.0
98	37.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33491406839368093
2	0.3369168767761016
3	0.35071400118833296
4	0.3524942753060402
5	0.3585027004533023
6	0.2981959147159684
7	0.3060587920691755
8	0.3042785179514682
9	0.30494612074560845
10	0.4209606507495325
11	0.30249824383376095
12	0.30413016177499264
13	0.2982700928042062
14	0.3030916685396634
15	0.2942644760393648
16	0.2993085860395354
17	0.2935968732452246
18	0.29545132545116964
19	0.29656399677473677
20	0.4920974373695856
21	0.29812173662773056
22	0.2982700928042062
23	0.2935968732452246
24	0.2973799557453526
25	0.2905555716274747
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1348107.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.43811123208552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41564483776592	66.55931716322273
2	6.520089041013621	9.706862265561474
3	1.6886087809368817	3.7709054478856765
4	0.7318152625830102	2.1789978366996787
5	0.40494911459852134	1.5071823617909632
6	0.24505403361567973	1.0944815647293116
7	0.17599052290149653	0.9170281483674143
8	0.12317794347490989	0.7335306766179908
9	0.09792357010791958	0.6560321043542616
>10	0.5455143139702306	7.500680898473261
>50	0.03470962641537249	1.7589464697223107
>100	0.015407934299615705	1.852288090887391
>500	3.0409590457965385E-4	0.17724242295162076
>1k	8.109224122124103E-4	1.5865045487359632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4927	0.36547544074765576	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3667	0.2720110495680239	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2884	0.2139296064778241	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2868	0.21274275706601925	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2248	0.16675234235858133	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1652	0.1225422017688507	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1467	0.10881925544485713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.4835617647560617E-4
7	0.0	0.0	0.0	0.0	1.4835617647560617E-4
8	0.0	0.0	0.0	0.0	1.4835617647560617E-4
9	0.0	0.0	0.0	0.0	1.4835617647560617E-4
10	0.0	0.0	0.0	0.0	1.4835617647560617E-4
11	0.0	0.0	0.0	1.4835617647560617E-4	1.4835617647560617E-4
12	0.0	0.0	0.0	1.4835617647560617E-4	2.2253426471340924E-4
13	0.0	0.0	0.0	1.4835617647560617E-4	2.2253426471340924E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	60	7.7334334E-8	17.406631	2
ATCACGC	80	1.2970668E-7	14.241791	3
ATAACGC	125	1.03682396E-10	12.912557	3
CTAAGCT	60	4.0727382E-4	12.673525	4
GGTATCA	1215	0.0	12.580756	1
GTACTAA	105	2.7403257E-7	11.75469	1
TTAGACT	115	7.016388E-8	11.57148	4
TAACGCA	165	7.2759576E-12	11.521386	4
ACTAGCC	75	2.0845416E-4	11.393432	3
AGACCGT	85	5.3142554E-5	11.177939	6
GTATCAC	120	1.2852615E-7	11.076536	1
ATAAGAC	140	8.418283E-9	10.850888	3
AACGCAT	215	0.0	10.606045	6
GTATCAA	2780	0.0	10.587028	1
GTTATCA	245	0.0	10.462966	1
TATAACG	155	3.6925485E-9	10.413352	2
TAGGGTG	130	3.8097642E-7	10.236308	5
AGGACCG	75	0.0026353074	10.13882	5
GGACCGA	85	6.583737E-4	10.060145	6
GTATTAG	190	1.0004442E-10	9.993867	1
>>END_MODULE