FastQCFastQC Report
Thu 26 May 2016
SRR1364296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348107
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT108710.8063899972331572No Hit
GGTATCAACGCAGAGTACTTTTTTT78580.5828914173726566No Hit
TATCAACGCAGAGTACTTTTTTTTT76510.5675365531074313No Hit
GTACTTTTTTTTTTTTTTTTTTTTT65730.48757257398707965No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39850.29559968162764527No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38610.2864015986861577No Hit
GTGGTATCAACGCAGAGTACATGGG17420.12921822971025296No Hit
GTGGTATCAACGCAGAGTACTTTTT15370.11401172162150335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG851.020453E-915.6462032
CTCGCCG400.005274038814.2518659
TATAACG2450.013.5706862
ATAACGC2600.013.5194943
TAACGCA2700.013.372124
TTGCGCG500.001502503213.29631318
TGGTATC6000.013.1409482
GGTATCA18250.012.963821
GTATAAC2900.012.7780081
ATCACGC1051.9919753E-812.6669143
GTATCAC1405.2750693E-1112.2163381
TCGAGCC658.0383895E-411.68949913
GTCTAGG1552.5465852E-1111.6471181
CTACACG907.447534E-611.6126314
TCACGCA1157.0565875E-811.56673054
TCGTTCG1157.092967E-811.56243914
CCTTATA1251.8319042E-811.3993762
GTGGTAT7150.011.2956041
TCGCGCA1104.98354E-711.22415918
CAACGCT600.0058692511.0847844
Produced by FastQC (version 0.11.5)