##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364296_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1348107 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.52251564601326 31.0 30.0 34.0 26.0 34.0 2 29.534809180576914 31.0 30.0 34.0 25.0 34.0 3 29.580165372629917 31.0 30.0 34.0 25.0 34.0 4 32.93944175054354 35.0 33.0 37.0 26.0 37.0 5 32.50750793520099 35.0 33.0 37.0 25.0 37.0 6 32.412289974015415 35.0 32.0 37.0 24.0 37.0 7 32.12963511056615 35.0 32.0 37.0 22.0 37.0 8 32.01687328973145 35.0 32.0 37.0 19.0 37.0 9 32.73513823457633 37.0 32.0 39.0 16.0 39.0 10 32.837026289456254 37.0 32.0 39.0 17.0 39.0 11 32.79375746880626 37.0 32.0 39.0 17.0 39.0 12 32.75136617494012 37.0 32.0 39.0 17.0 39.0 13 32.80064342073737 37.0 32.0 39.0 17.0 39.0 14 33.70843560637249 38.0 32.0 41.0 17.0 41.0 15 33.7439290798134 38.0 33.0 41.0 17.0 41.0 16 33.68755595809532 38.0 32.0 41.0 17.0 41.0 17 33.65204690725588 38.0 32.0 41.0 16.0 41.0 18 33.587953330114004 38.0 32.0 41.0 15.0 41.0 19 33.49445333345202 38.0 32.0 41.0 10.0 41.0 20 33.40476163984016 38.0 32.0 41.0 10.0 41.0 21 33.344319849982234 38.0 32.0 41.0 10.0 41.0 22 33.29890802436305 38.0 32.0 41.0 2.0 41.0 23 33.040361781371956 38.0 32.0 41.0 2.0 41.0 24 32.95814798083535 38.0 32.0 41.0 2.0 41.0 25 32.787945615592825 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3103443923812428 1101 2 0.5456274005759632 1101 3 -1.2371379802927898 1101 4 -0.4060087673433799 1101 5 0.48887265069801344 1101 6 0.7784267721823568 1101 7 0.9072206950830513 1101 8 1.0661772856017038 1101 9 1.7886810404254803 1101 10 1.431863944134605 1101 11 1.3793422108450102 1101 12 1.5043574895285161 1101 13 1.2621584392520262 1101 14 1.514325019391805 1101 15 1.4746494019683567 1101 16 1.4971250089427883 1101 17 1.3704055395647288 1101 18 1.3863219107343383 1101 19 1.5746316498109678 1101 20 1.659898611112176 1101 21 1.7273657311381925 1101 22 1.757368838359973 1101 23 1.9743887120794312 1101 24 1.7885505292083437 1101 25 2.1174941631380406 1102 1 0.9759136750697159 1102 2 0.893875716150557 1102 3 0.9813712294727495 1102 4 1.0050736308536017 1102 5 1.2811366836431333 1102 6 1.3263399726496345 1102 7 1.3901522442824295 1102 8 1.3984840339231823 1102 9 2.1280558855527403 1102 10 1.6917315500675585 1102 11 1.824360913571951 1102 12 1.7835414134432312 1102 13 1.7772799184784276 1102 14 1.8618299458198777 1102 15 1.7908139859654284 1102 16 1.7338649525396121 1102 17 1.7296158950935379 1102 18 1.8654067160961745 1102 19 1.7652224671850334 1102 20 1.8502770315872326 1102 21 2.123568546577083 1102 22 2.2278987686229925 1102 23 2.558845979003337 1102 24 2.5626074279776176 1102 25 2.4921032938447354 1103 1 -1.9721540932249013 1103 2 -1.9693966061239614 1103 3 -2.2498507577114495 1103 4 -1.7227549142782088 1103 5 -4.147628103449797 1103 6 -8.465807351075071 1103 7 -9.73216248925019 1103 8 -11.76304380777033 1103 9 -11.962950236178049 1103 10 -9.88196318323303 1103 11 -11.83803124071268 1103 12 -10.789971562847146 1103 13 -12.818964285599886 1103 14 -13.172669081586758 1103 15 -11.887642394285702 1103 16 -6.941049099597578 1103 17 -4.756982425781061 1103 18 -3.7522461434829957 1103 19 -3.287560542014962 1103 20 -8.316367076585255 1103 21 -5.181315302959046 1103 22 -11.220043316632648 1103 23 -12.470668265718945 1103 24 -13.902899345371388 1103 25 -17.159812695744844 1104 1 0.13226487547472132 1104 2 0.4074345712033214 1104 3 0.4253581581069632 1104 4 0.6116817334114515 1104 5 0.5457154063359511 1104 6 0.60013450296033 1104 7 0.5302614794104947 1104 8 0.8541943757329165 1104 9 1.1286534117686173 1104 10 0.8687202226594479 1104 11 0.9441005183857669 1104 12 0.9492866983813499 1104 13 0.6930722250191863 1104 14 1.0221269090986738 1104 15 0.9046917775665406 1104 16 0.43130002540923584 1104 17 0.38659511644991795 1104 18 0.5398037626703811 1104 19 1.0517942916701344 1104 20 1.297954462966615 1104 21 0.9731616870955477 1104 22 0.7965648094722866 1104 23 1.429480487016626 1104 24 1.4895898096974989 1104 25 1.6697758588614278 1105 1 0.24145214134813742 1105 2 0.3975224387217011 1105 3 -0.11353880718866449 1105 4 0.29893296879581044 1105 5 0.5718161098791015 1105 6 0.6046089342509404 1105 7 0.7673799019637251 1105 8 0.5009825948699316 1105 9 0.535538268134566 1105 10 0.21679711296465598 1105 11 0.7006991029099154 1105 12 1.0415729361405681 1105 13 0.8026625829693259 1105 14 0.552821114491671 1105 15 0.3542246495615373 1105 16 0.6144047651166247 1105 17 0.615788464753372 1105 18 0.6193321908210194 1105 19 0.8873014820025631 1105 20 0.8867557567064779 1105 21 0.9165575095322893 1105 22 0.8665418124684976 1105 23 1.187484104139891 1105 24 1.1993276840542393 1105 25 1.4405145638718722 1106 1 0.07302855618718596 1106 2 0.29859378773583245 1106 3 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0.44812600398269353 1107 16 0.4329482266056317 1107 17 0.1293454008041195 1107 18 0.02576903986307144 1107 19 0.5080173482320447 1107 20 0.26042102501902065 1107 21 0.37962100338167204 1107 22 0.2538792069222282 1107 23 0.4889780928244747 1107 24 0.7978065051506391 1107 25 0.457269364351923 1108 1 -0.30489687694807444 1108 2 -0.28015338746897456 1108 3 -0.17588441903922813 1108 4 -0.3466187048086624 1108 5 -0.25661642812439567 1108 6 -0.14704223819231288 1108 7 -0.4505620998066604 1108 8 -0.08498112970765348 1108 9 0.1520632906001822 1108 10 0.028201452421804163 1108 11 -0.09106578786487773 1108 12 0.004796486822861823 1108 13 -0.5745166489953881 1108 14 -0.38626449126059725 1108 15 -0.6803435203201644 1108 16 -1.0492832764592066 1108 17 -0.9862752825249004 1108 18 -1.207516000713639 1108 19 -1.0204605396208422 1108 20 -0.3133225539328919 1108 21 -0.49734712879479304 1108 22 -0.49254604991969586 1108 23 -0.816666494070116 1108 24 -0.2859625421600782 1108 25 -0.5672520247528006 1109 1 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0.4810913277118729 2210 21 0.5811072607212111 2210 22 0.7252393844210445 2210 23 1.0217499933441445 2210 24 0.9207124155913462 2210 25 1.0129035176893026 2211 1 0.026029159404316715 2211 2 0.02813522314521677 2211 3 0.19637424391710923 2211 4 0.23387114699901446 2211 5 -0.5943778627850556 2211 6 0.20404767103713084 2211 7 0.2918460805860157 2211 8 0.4893248399471517 2211 9 1.00600220267863 2211 10 0.33464594103683254 2211 11 0.6475216367690635 2211 12 0.6502060585890206 2211 13 0.4527697310041674 2211 14 0.5289500673648106 2211 15 0.5525569412624662 2211 16 0.5786347429316834 2211 17 0.6058314336128277 2211 18 0.523805362390668 2211 19 0.3879075780495995 2211 20 0.8016093129369608 2211 21 1.0421192905165881 2211 22 0.843459603915413 2211 23 1.0824589283111195 2211 24 1.1115948243895346 2211 25 1.2959737532016575 2212 1 0.05239726850668447 2212 2 0.11672482207530877 2212 3 0.023723685351871637 2212 4 0.01215587909139515 2212 5 -0.09815694208888459 2212 6 0.013906234979366161 2212 7 0.022710939306353595 2212 8 -0.12929712929380344 2212 9 0.5331458107438181 2212 10 0.13400990219246012 2212 11 0.08317887309369354 2212 12 0.21526099961982226 2212 13 -0.020883139944103846 2212 14 0.1385269645907954 2212 15 0.3323373602378936 2212 16 0.36700592046181413 2212 17 0.010551868716184742 2212 18 -0.059147469115110596 2212 19 0.21293815170521668 2212 20 0.3541898973114783 2212 21 0.48403888123012706 2212 22 0.24279655802403255 2212 23 0.4816246089154106 2212 24 0.5876626898944437 2212 25 0.6498984340569436 2213 1 -0.013317318585094995 2213 2 0.03445186846727921 2213 3 -0.0412715291723984 2213 4 0.09323207596447247 2213 5 0.08336499431470656 2213 6 0.24136641159987704 2213 7 0.07831669366640881 2213 8 0.18660592953264654 2213 9 0.7896399417495132 2213 10 0.22580882224166032 2213 11 0.3256981739954057 2213 12 0.5266151418872838 2213 13 0.15570789500615234 2213 14 0.26550145808270287 2213 15 0.2029255316587566 2213 16 0.21911882046011044 2213 17 0.1383581973333321 2213 18 -0.15568843108104602 2213 19 -0.09446351336112002 2213 20 0.49263673509703665 2213 21 0.17281426480501239 2213 22 0.3499908266218483 2213 23 -0.06004044190991209 2213 24 0.19125582983354406 2213 25 0.4621169868111892 2214 1 -0.9278796670365139 2214 2 -0.7649566211747576 2214 3 -0.6199749267820636 2214 4 -0.6426094232459718 2214 5 -0.9193576908071286 2214 6 -0.7465047046433639 2214 7 -0.8194571085904627 2214 8 -0.6497865645745442 2214 9 -10.443509624112327 2214 10 -3.973584906549224 2214 11 -1.8726480725200325 2214 12 -1.264636055509886 2214 13 -0.9413147183102843 2214 14 -1.0755914445617947 2214 15 -0.8984915100514144 2214 16 -1.0565012506072335 2214 17 -1.1120557984136994 2214 18 -1.4223201432321488 2214 19 -1.640815276744675 2214 20 -1.2698350309311728 2214 21 -1.7763234554737828 2214 22 -1.628233458360981 2214 23 -1.9072667857239622 2214 24 -2.342570736315828 2214 25 -9.920066050818217 2215 1 -0.5883549921891351 2215 2 -0.3912101687583167 2215 3 -0.2261063762379294 2215 4 -0.34793763853070203 2215 5 -0.49408501179452813 2215 6 -0.46294629967776757 2215 7 -0.5262762277688537 2215 8 -0.7156019473052133 2215 9 0.25837085039611907 2215 10 0.2271007213985925 2215 11 0.3635595351325378 2215 12 0.11242828081124401 2215 13 0.03893682833405876 2215 14 -0.19351332626136752 2215 15 -0.5437221285049745 2215 16 -0.2895306317453219 2215 17 -0.48794978947072565 2215 18 -0.5710266789529967 2215 19 -0.5542285115283505 2215 20 -0.35062152254968737 2215 21 -0.4005335949269977 2215 22 -0.28775486636882164 2215 23 0.08945289902602127 2215 24 0.14570335152131975 2215 25 0.35233895561337647 2216 1 -1.1097208877527365 2216 2 -1.0821213794105837 2216 3 -1.2022553607136004 2216 4 -1.0344774924915505 2216 5 -2.0145844902261416 2216 6 -2.69874675374907 2216 7 -2.1419183704945084 2216 8 -3.150378900118614 2216 9 -1.667670752352855 2216 10 -2.086078391321543 2216 11 -1.5260115515705337 2216 12 -2.5448816535324994 2216 13 -2.4874594409179522 2216 14 -2.6533856954090673 2216 15 -2.383609224289472 2216 16 -3.3285083577071575 2216 17 -3.852878303921237 2216 18 -3.363829035214888 2216 19 -4.947519301244245 2216 20 -3.7383444163338204 2216 21 -3.6218908999524615 2216 22 -3.49430331086781 2216 23 -3.0496487761289153 2216 24 -3.1339984340833915 2216 25 -3.2168136491605104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50703.0 3 1.0 4 727.0 5 7057.0 6 9284.0 7 7392.0 8 5923.0 9 4943.0 10 4366.0 11 3722.0 12 3381.0 13 3202.0 14 3120.0 15 3243.0 16 3345.0 17 3570.0 18 3902.0 19 4853.0 20 6351.0 21 8421.0 22 10503.0 23 12710.0 24 14863.0 25 16901.0 26 19027.0 27 20918.0 28 23048.0 29 27006.0 30 33009.0 31 40630.0 32 50140.0 33 62076.0 34 76261.0 35 95096.0 36 123305.0 37 179955.0 38 405153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59279177406884 18.606435375421633 11.989644871629352 24.81112797888017 2 17.002362593140692 20.74883446641372 37.62316453959105 24.625638400854537 3 19.75681455552119 25.61621592351349 29.062529903041824 25.5644396179235 4 12.596351637950026 16.44989789007438 36.116870429069394 34.8368800429062 5 12.806446705969213 38.227749477800494 34.22133838899133 14.744465427238964 6 31.922616963315985 36.70100538301295 17.28447175847068 14.091905895200393 7 27.852181932440484 32.265979334020365 21.403467379522027 18.47837135401712 8 26.715079761402144 34.99744380640599 19.605077364738772 18.68239906745309 9 26.41939689684676 15.316448463450127 19.567867841795433 38.69628679790768 10 15.477684365661313 29.949545583735855 32.07985140473139 22.492918645871445 11 35.058505917379314 22.903389543736957 22.260002552769606 19.778101986114123 12 23.085134635899244 26.16998737394344 29.845416118325886 20.89946187183143 13 28.840469085085278 21.80708582856541 25.893888378282746 23.458556708066563 14 21.93622905434076 21.180746165464402 27.88995943656288 28.993065343631958 15 24.169402806812432 28.75372958280601 24.10033783232419 22.976529778057365 16 23.02789808142269 28.09163057557319 25.889888410641714 22.990582932362397 17 22.116861207841787 27.585725964755582 27.257154896517548 23.040257930885087 18 21.547715936768995 26.636881687802276 29.389568679180613 22.425833696248116 19 22.99493052376674 26.216752196612287 29.191517585881737 21.59679969373924 20 23.035330881098254 25.696535166920643 29.317019391847676 21.951114560133426 21 23.418125327354765 25.27928091007963 29.243519302988442 22.059074459577158 22 23.004904510057177 25.301885910606014 29.314889427817054 22.37832015151976 23 22.508594379416813 25.37563780903786 29.579724831487848 22.53604298005748 24 22.387939015560647 25.781964012296587 29.589337218549332 22.240759753593426 25 22.403047065699198 25.297209950638905 29.664408398292895 22.635334585369005 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 814.0 1 814.0 2 1187.0 3 1560.0 4 1560.0 5 1560.0 6 4925.0 7 8290.0 8 8290.0 9 8290.0 10 8075.0 11 7860.0 12 7860.0 13 7860.0 14 7898.0 15 7936.0 16 7936.0 17 7936.0 18 12680.5 19 17425.0 20 17425.0 21 17425.0 22 25474.5 23 33524.0 24 33524.0 25 33524.0 26 46993.5 27 60463.0 28 60463.0 29 60463.0 30 72885.5 31 85308.0 32 85308.0 33 85308.0 34 96447.5 35 107587.0 36 107587.0 37 107587.0 38 116975.5 39 126364.0 40 126364.0 41 126364.0 42 136527.5 43 146691.0 44 146691.0 45 146691.0 46 156471.0 47 166251.0 48 166251.0 49 166251.0 50 166770.5 51 167290.0 52 167290.0 53 167290.0 54 155723.5 55 144157.0 56 144157.0 57 144157.0 58 128295.5 59 112434.0 60 112434.0 61 112434.0 62 94392.0 63 76350.0 64 76350.0 65 76350.0 66 59195.5 67 42041.0 68 42041.0 69 42041.0 70 31526.5 71 21012.0 72 21012.0 73 21012.0 74 14743.0 75 8474.0 76 8474.0 77 8474.0 78 6196.5 79 3919.0 80 3919.0 81 3919.0 82 2696.5 83 1474.0 84 1474.0 85 1474.0 86 1019.5 87 565.0 88 565.0 89 565.0 90 426.5 91 288.0 92 288.0 93 288.0 94 157.5 95 27.0 96 27.0 97 27.0 98 15.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027371714559749336 2 8.90137058853637E-4 3 0.0 4 0.003708904411890154 5 0.03278671500110896 6 0.012091028382761902 7 0.021363289412487288 8 0.029671235295121234 9 0.03783082500127957 10 0.04109466088374291 11 0.040872126619029496 12 0.007491986912018112 13 0.002670411176560911 14 0.007046918382591293 15 0.007491986912018112 16 0.009568973382676598 17 0.011052535147432659 18 0.01661589176526789 19 0.017506028824121528 20 0.01639335750055448 21 0.013426233971042359 22 0.012165206470999706 23 0.00986568573562781 24 0.00675020602964008 25 0.11126713235670461 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1348107.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.84881490503465 #Duplication Level Percentage of deduplicated Percentage of total 1 80.67186237141621 47.47443496739903 2 11.639548686680499 13.699472924811998 3 3.2321838776679206 5.706305722677498 4 1.3441268810371494 3.1640109612414693 5 0.7405964925033108 2.179161295332261 6 0.4400267438349541 1.5537031440724989 7 0.3136504940328435 1.292057190774804 8 0.22243778612699971 1.0472160082938793 9 0.18000917158159596 0.9533993777652569 >10 1.0934134783184901 12.114475291763604 >50 0.08305114727869914 3.3730519289735734 >100 0.03706865932668681 3.6725625938997997 >500 6.325656858262961E-4 0.23109739988258537 >1k 8.855919601568146E-4 1.0856515093710888 >5k 3.795394114957777E-4 1.644037623778109 >10k+ 1.265131371652592E-4 0.8093620599624953 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 10871 0.8063899972331572 No Hit GGTATCAACGCAGAGTACTTTTTTT 7858 0.5828914173726566 No Hit TATCAACGCAGAGTACTTTTTTTTT 7651 0.5675365531074313 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 6573 0.48757257398707965 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3985 0.29559968162764527 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3861 0.2864015986861577 No Hit GTGGTATCAACGCAGAGTACATGGG 1742 0.12921822971025296 No Hit GTGGTATCAACGCAGAGTACTTTTT 1537 0.11401172162150335 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4835617647560617E-4 2 0.0 0.0 0.0 0.0 1.4835617647560617E-4 3 0.0 0.0 0.0 0.0 1.4835617647560617E-4 4 0.0 0.0 0.0 0.0 1.4835617647560617E-4 5 0.0 0.0 0.0 0.0 1.4835617647560617E-4 6 0.0 0.0 0.0 0.0 1.4835617647560617E-4 7 0.0 0.0 0.0 0.0 1.4835617647560617E-4 8 0.0 0.0 0.0 0.0 1.4835617647560617E-4 9 0.0 0.0 0.0 7.417808823780308E-5 1.4835617647560617E-4 10 0.0 0.0 0.0 7.417808823780308E-5 1.4835617647560617E-4 11 0.0 0.0 0.0 1.4835617647560617E-4 1.4835617647560617E-4 12 0.0 0.0 0.0 1.4835617647560617E-4 2.2253426471340924E-4 13 0.0 0.0 0.0 2.9671235295121234E-4 2.2253426471340924E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 85 1.020453E-9 15.646203 2 CTCGCCG 40 0.0052740388 14.251865 9 TATAACG 245 0.0 13.570686 2 ATAACGC 260 0.0 13.519494 3 TAACGCA 270 0.0 13.37212 4 TTGCGCG 50 0.0015025032 13.296313 18 TGGTATC 600 0.0 13.140948 2 GGTATCA 1825 0.0 12.96382 1 GTATAAC 290 0.0 12.778008 1 ATCACGC 105 1.9919753E-8 12.666914 3 GTATCAC 140 5.2750693E-11 12.216338 1 TCGAGCC 65 8.0383895E-4 11.689499 13 GTCTAGG 155 2.5465852E-11 11.647118 1 CTACACG 90 7.447534E-6 11.612631 4 TCACGCA 115 7.0565875E-8 11.5667305 4 TCGTTCG 115 7.092967E-8 11.562439 14 CCTTATA 125 1.8319042E-8 11.399376 2 GTGGTAT 715 0.0 11.295604 1 TCGCGCA 110 4.98354E-7 11.224159 18 CAACGCT 60 0.00586925 11.084784 4 >>END_MODULE