FastQCFastQC Report
Thu 26 May 2016
SRR1364295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939795
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN74140.25219445573585914No Hit
GTATCAACGCAGAGTACTTTTTTTT67770.23052627819286717No Hit
TATCAACGCAGAGTACTTTTTTTTT38390.13058733687212884No Hit
GGTATCAACGCAGAGTACTTTTTTT31920.10857899955609149No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC456.768776E-414.7766488
GGTATCA17400.012.6042161
AAGACCG1251.823173E-811.4045845
TCGTCGC951.3792334E-510.98696113
GTTCGCG1752.0008883E-1110.86652216
TCGGTTT1158.96056E-710.726413
GTATAGG4350.010.6944861
GTATCAA40650.010.6268361
TCGCGCA2001.8189894E-1210.45884918
GTACTAG2300.010.3196571
CGATCCA1201.5084861E-610.30038118
CGGGCGA750.00263023410.14191318
ACCGATA750.002650446710.1325598
TATAACG1606.2464096E-910.0871212
CGATTAG856.545523E-410.0673414
TTCGCGC1909.822543E-1110.00846817
TAGGACC3150.09.9567234
ACCGACC1054.1110263E-59.951628
TCGAACT2900.09.83573919
TCGTTCG1951.6189006E-109.75184114
Produced by FastQC (version 0.11.5)