FastQCFastQC Report
Thu 26 May 2016
SRR1364295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939795
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT136920.46574676125376085No Hit
GGTATCAACGCAGAGTACTTTTTTT99860.3396835493631359No Hit
TATCAACGCAGAGTACTTTTTTTTT88910.30243605421466463No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62580.21287198597181095No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39040.13279837539692393No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34670.11793339331483997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC801.2878627E-714.2516988
GGTATCA29400.013.11949351
TATAACG1850.012.8382752
TAACGCA2050.012.5145384
CGCGCCA1155.329639E-912.391726510
CGCGGTT701.0943159E-412.21096118
TTTACGG1103.8329745E-812.08761818
GTTCGCG2300.011.97540816
TGGTCGG1353.7107384E-1011.96337110
TGGTATC9300.011.952022
TCGCGCA2400.011.87176818
ATAACGC2100.011.7633083
TGGACCG1302.6029738E-911.6966875
TTCGCGC2450.011.629683517
CTACACG2050.011.5875354
GTGGTAT10800.011.43557551
CGTTCGC2550.011.17380815
TCGTTCG2400.011.08088214
TCGCGGT951.3650822E-510.99719317
CTTACAC4950.010.9406983
Produced by FastQC (version 0.11.5)