##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364295_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2939795
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.775656125682232	31.0	30.0	34.0	26.0	34.0
2	29.80616437540713	31.0	30.0	34.0	26.0	34.0
3	29.85045521881628	31.0	30.0	34.0	26.0	34.0
4	33.239824545589066	35.0	33.0	37.0	27.0	37.0
5	32.83377242290704	35.0	33.0	37.0	26.0	37.0
6	32.756601395675546	35.0	33.0	37.0	26.0	37.0
7	32.48581244610594	35.0	33.0	37.0	25.0	37.0
8	32.37977512037404	35.0	33.0	37.0	23.0	37.0
9	33.1441413431889	37.0	32.0	39.0	17.0	39.0
10	33.27475079044627	37.0	32.0	39.0	19.0	39.0
11	33.23969120295803	37.0	33.0	39.0	18.0	39.0
12	33.127671827457355	37.0	32.0	39.0	17.0	39.0
13	33.241253216635855	37.0	33.0	39.0	18.0	39.0
14	34.20394381240869	38.0	33.0	41.0	18.0	41.0
15	34.24465073244903	38.0	33.0	41.0	19.0	41.0
16	34.17518228311838	38.0	33.0	41.0	18.0	41.0
17	34.113536147928684	38.0	33.0	41.0	18.0	41.0
18	34.04896463869079	38.0	33.0	41.0	17.0	41.0
19	33.935567275949516	39.0	33.0	41.0	18.0	41.0
20	33.77692560195524	39.0	33.0	41.0	17.0	41.0
21	33.70494745381906	39.0	33.0	41.0	16.0	41.0
22	33.68589340413192	39.0	33.0	41.0	11.0	41.0
23	33.397247086956746	38.0	32.0	41.0	8.0	41.0
24	33.307657846890685	38.0	32.0	41.0	2.0	41.0
25	33.17215282017964	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.29315487772520754
1101	2	0.5595190366068259
1101	3	-1.5185026991883248
1101	4	-0.3970912085246283
1101	5	0.5232214061936915
1101	6	0.7697730601524881
1101	7	0.9108165546072442
1101	8	1.0378755202384653
1101	9	1.7622697478603513
1101	10	1.4556541281474864
1101	11	1.3731241295958583
1101	12	1.4741513516296507
1101	13	1.3788708812099273
1101	14	1.3062573660417698
1101	15	1.4183453133425346
1101	16	1.3473344967395846
1101	17	1.3452814922252472
1101	18	1.3511458513556676
1101	19	1.2619392640443863
1101	20	1.5302298072678084
1101	21	1.413286536486126
1101	22	1.4648979012298895
1101	23	1.7498273195997243
1101	24	1.7396012962344898
1101	25	1.9033351979334157
1102	1	0.6757274349699571
1102	2	0.5655714498200517
1102	3	0.7013317725654353
1102	4	0.7156063165650437
1102	5	0.9058862913383621
1102	6	0.9365712505256454
1102	7	1.1326717879442327
1102	8	1.204378145196145
1102	9	1.7463219903540619
1102	10	1.259222165970563
1102	11	1.357734404239423
1102	12	1.4357835927645795
1102	13	1.2211533430317587
1102	14	1.233345616997127
1102	15	1.3120909981745754
1102	16	1.3816636358312735
1102	17	1.269788046054444
1102	18	1.2895358502117347
1102	19	1.16692630620107
1102	20	1.5268624519743668
1102	21	1.5822843374562083
1102	22	1.535587973890209
1102	23	1.8770272931207828
1102	24	1.9766372106262011
1102	25	1.759840221093782
1103	1	-2.919979109636781
1103	2	-2.704924169246567
1103	3	-2.5825899269630277
1103	4	-2.5190150150187307
1103	5	-5.986316913374193
1103	6	-10.075326523006122
1103	7	-11.106311932709396
1103	8	-12.408125707544126
1103	9	-12.454895283734675
1103	10	-10.26271831063099
1103	11	-12.471727005051402
1103	12	-11.57007576974599
1103	13	-13.134252195450241
1103	14	-12.221798254875758
1103	15	-12.051471478884835
1103	16	-6.7881382981484215
1103	17	-4.897576270344736
1103	18	-4.262753988401613
1103	19	-3.791797256966607
1103	20	-9.190391813536667
1103	21	-5.6992080574583035
1103	22	-11.040088158048082
1103	23	-12.398541793386507
1103	24	-13.651574312168595
1103	25	-14.543257573923807
1104	1	0.3117555301631434
1104	2	0.5389597885978112
1104	3	0.5985572006625581
1104	4	0.7079005749379377
1104	5	0.7175601408653023
1104	6	0.7225877670710332
1104	7	0.6650863552479152
1104	8	0.9580970581837107
1104	9	1.2697317227056928
1104	10	1.0717138678669755
1104	11	0.8995701669086742
1104	12	0.9739699993579407
1104	13	0.9436141800160414
1104	14	1.0693349757161954
1104	15	1.071929175289469
1104	16	0.6061460816114419
1104	17	0.5286995171217583
1104	18	0.8568625150636606
1104	19	1.02191127488814
1104	20	1.330999737631494
1104	21	1.0800408354626754
1104	22	0.818405264920905
1104	23	1.3426326920190377
1104	24	1.563881678633443
1104	25	1.654489002183304
1105	1	0.2987960390343254
1105	2	0.4339931706641771
1105	3	-0.005185583362937507
1105	4	0.41479237620836784
1105	5	0.4309459183269766
1105	6	0.48792401785360795
1105	7	0.6241256672320716
1105	8	0.5031236523078846
1105	9	0.6946106265296379
1105	10	0.3010770092339783
1105	11	0.8400224540888672
1105	12	1.1160831855324886
1105	13	0.9126941679866007
1105	14	0.5557659950798239
1105	15	0.556173978415984
1105	16	0.8898528913249351
1105	17	0.7600647451128779
1105	18	0.716791323624804
1105	19	0.7365599926440822
1105	20	0.9609901222708572
1105	21	1.0181626849043397
1105	22	0.8076559081719594
1105	23	1.2328753636248138
1105	24	1.2007134983685006
1105	25	1.381178834558547
1106	1	-0.163061864747025
1106	2	0.1032763290522496
1106	3	-0.024531417037234604
1106	4	-1.4800204534413197
1106	5	-0.38071373069141856
1106	6	-0.08313320449673256
1106	7	0.27062031472543424
1106	8	0.5196624886677341
1106	9	0.9487723027308874
1106	10	0.455366453748681
1106	11	0.6301844799326588
1106	12	0.7912833000962181
1106	13	0.4770144479982008
1106	14	-0.052274853584464154
1106	15	0.4507186247934669
1106	16	0.2551949458840497
1106	17	0.18098750386695883
1106	18	0.23712545543260433
1106	19	-1.1763700862858641
1106	20	0.12006576408811043
1106	21	-0.2542888300893722
1106	22	0.33328768249629803
1106	23	0.25917648024919515
1106	24	0.38978716879692854
1106	25	0.6979069986376132
1107	1	-0.03851451031280462
1107	2	-0.15034119569593685
1107	3	-0.32321432417866447
1107	4	-0.17375307810832652
1107	5	-0.11609177462860742
1107	6	-0.12002286209037294
1107	7	-0.16433846363603521
1107	8	-0.3870869960041965
1107	9	0.4624075564234147
1107	10	0.29560014593034367
1107	11	0.2988608723320638
1107	12	0.09768502470600282
1107	13	0.05195816965746047
1107	14	0.2262525751380835
1107	15	-0.03643180677363489
1107	16	-0.12927573363026568
1107	17	-0.28940649519867634
1107	18	-0.26917182136836715
1107	19	-0.005504298264696672
1107	20	-0.07239819059066832
1107	21	-0.051434783347254154
1107	22	-0.03628017909262127
1107	23	0.21583091603291393
1107	24	0.45458240593998767
1107	25	0.16240824307312351
1108	1	0.036917904392133494
1108	2	0.12061532193709468
1108	3	0.14002392391002516
1108	4	-0.06748609914932047
1108	5	0.2465276960041578
1108	6	0.3296525086983664
1108	7	0.013227461945028551
1108	8	0.40594825530284595
1108	9	0.6338499434307963
1108	10	0.45971856997307015
1108	11	0.5105317254818686
1108	12	0.5754702103475395
1108	13	0.11049232383415841
1108	14	0.22691522489600402
1108	15	-0.05020989537631948
1108	16	-0.5483328581896103
1108	17	-0.6112745735752512
1108	18	-0.5898768272192356
1108	19	-0.4282172020521955
1108	20	0.16694467666025048
1108	21	-0.05480299932624888
1108	22	0.11365878519272599
1108	23	-0.2877436574733423
1108	24	0.19907618763834733
1108	25	0.033589084019475024
1109	1	0.29302830699315763
1109	2	0.37746633704860955
1109	3	0.27233590019849885
1109	4	0.31394406469663494
1109	5	0.34026786651367047
1109	6	0.7141528742995149
1109	7	0.7346522945734932
1109	8	0.579487304188298
1109	9	1.3779133970311293
1109	10	0.6702142204002328
1109	11	0.7309061536011825
1109	12	0.7885779896392293
1109	13	0.8296461714787284
1109	14	0.4938404894630324
1109	15	0.3246063257613159
1109	16	0.6324438634899039
1109	17	0.612474956845297
1109	18	0.46997726960059083
1109	19	0.7258478093593297
1109	20	1.0514510051750534
1109	21	1.0378644989184167
1109	22	0.624133108886511
1109	23	0.9552128836938678
1109	24	0.7267607845379302
1109	25	0.7670278832824593
1110	1	-0.10621870038240289
1110	2	0.01501287840361698
1110	3	-0.06319778254443875
1110	4	0.028379385940297652
1110	5	-0.0077585350062108205
1110	6	0.14011539351469793
1110	7	0.1922027251875349
1110	8	0.06668694190359759
1110	9	-3.4047090550419945
1110	10	-1.3854189792593097
1110	11	-0.4894677721469378
1110	12	-0.10798821341261089
1110	13	-0.3965448918237513
1110	14	-0.015944070980530967
1110	15	-0.01965618184846818
1110	16	-0.4154757727463121
1110	17	-0.22093466922672178
1110	18	-0.1256069735097327
1110	19	-0.3522456161668188
1110	20	0.04837452554699695
1110	21	-0.17099040387572728
1110	22	0.13023000133291163
1110	23	0.26413169418649574
1110	24	0.41570969780634215
1110	25	0.444894947696028
1111	1	0.1513487529522557
1111	2	0.1431237424325147
1111	3	0.17173664791280174
1111	4	-0.09635049068146628
1111	5	0.27888318387967104
1111	6	0.21585489275800995
1111	7	0.4042862943372114
1111	8	0.48172721528184326
1111	9	1.0262000314849118
1111	10	0.5982691248386232
1111	11	0.7457936131538503
1111	12	0.7394365844536139
1111	13	0.5359780878080755
1111	14	0.2838832490086105
1111	15	0.5153571060328375
1111	16	0.38269917855117086
1111	17	0.41232265510296884
1111	18	0.4623038043215857
1111	19	0.4981163852034953
1111	20	0.7600383032580211
1111	21	0.6500876881996831
1111	22	0.7118327987447017
1111	23	0.9001671486946989
1111	24	0.8918348731759167
1111	25	1.142793185904175
1112	1	-0.19455038222321264
1112	2	-0.23070002937825507
1112	3	-0.2781039041442739
1112	4	-0.16199575615898
1112	5	-0.10149927702281758
1112	6	-0.18571631365574603
1112	7	-0.04765099783048754
1112	8	-0.4370959553884255
1112	9	0.08894509337888934
1112	10	-0.037029167583497724
1112	11	0.14975953198909053
1112	12	-0.5748445484231013
1112	13	-0.3733130864418399
1112	14	-1.2762568481290089
1112	15	-0.6591077377300465
1112	16	-0.6737246232872565
1112	17	-0.6126524848796322
1112	18	-0.6552948650637447
1112	19	-0.3159630346509701
1112	20	-0.3422048747687114
1112	21	-0.6673289537639704
1112	22	-0.2530982322995854
1112	23	-0.010749205846536825
1112	24	-0.09198936445661587
1112	25	-0.10121789821397442
1113	1	-1.933945063697795
1113	2	-1.9963540568070286
1113	3	-1.97413497606372
1113	4	-1.9590828556351596
1113	5	-1.7529263482451078
1113	6	-1.8692041167580875
1113	7	-2.1552325550004845
1113	8	-2.268558296166166
1113	9	-10.699843879810807
1113	10	-4.245980104219633
1113	11	-2.868293097306264
1113	12	-12.579292880657022
1113	13	-4.687716463965074
1113	14	-3.866543747871841
1113	15	-3.377156690049226
1113	16	-3.026744634126981
1113	17	-2.9804109193114243
1113	18	-3.3017853751559443
1113	19	-4.002348147898157
1113	20	-14.30258427987809
1113	21	-16.9688095985291
1113	22	-11.436168477987778
1113	23	-17.54916890031923
1113	24	-18.691799191248975
1113	25	-15.104309941016918
1114	1	-0.3197777910477271
1114	2	-0.2661907180571248
1114	3	-0.31505496881466755
1114	4	-0.8156225855245367
1114	5	-0.3489827422356768
1114	6	-0.2882717035590474
1114	7	-0.3901520609426612
1114	8	-0.18991039615456273
1114	9	0.3365920885329743
1114	10	-0.381819314770496
1114	11	-0.5749337907282026
1114	12	-0.5623767308139449
1114	13	-0.41402165363471966
1114	14	-0.456944364192978
1114	15	-0.32764528075299637
1114	16	-0.4304590912100892
1114	17	-0.4393140982030346
1114	18	-0.4454802537305227
1114	19	-0.6390861348301442
1114	20	-0.9199856461273797
1114	21	-1.1259325890211613
1114	22	-1.191302680246963
1114	23	-0.8355701020241213
1114	24	-0.44096058352206313
1114	25	-0.8295082094335697
1115	1	-0.26800739326177236
1115	2	-0.3031517213410382
1115	3	-0.309793475191551
1115	4	-0.18380795633242997
1115	5	-0.6503155580708722
1115	6	-0.7145839389870368
1115	7	-0.838954746407957
1115	8	-0.6878819751276595
1115	9	-0.21146558870569265
1115	10	-0.5467682235638591
1115	11	-0.4805003722302885
1115	12	-0.6005700944133423
1115	13	-0.6294392023128523
1115	14	-0.8708038566109906
1115	15	-0.9125862158643443
1115	16	-0.6389202359544441
1115	17	-0.6074566537178328
1115	18	-0.6669440419194288
1115	19	-0.6548269153357396
1115	20	-1.0347610559276674
1115	21	-0.5726885485891202
1115	22	-0.988664395248378
1115	23	-0.7565807139207408
1115	24	-0.4490731110032584
1115	25	-0.34299623367324017
1116	1	-0.8976433136982571
1116	2	-0.8438227251589758
1116	3	-0.8995583704838559
1116	4	-0.7101105263158871
1116	5	-1.808087934178733
1116	6	-1.683706009471738
1116	7	-2.8284120742346204
1116	8	-1.988634336605518
1116	9	-0.7697839361957861
1116	10	-1.9726711466042524
1116	11	-1.642656000487463
1116	12	-2.61946044988904
1116	13	-2.3320212673828387
1116	14	-1.8490201511811861
1116	15	-3.319307137275427
1116	16	-3.36529108904573
1116	17	-4.012524913591797
1116	18	-3.1335003599533877
1116	19	-2.7165651655419722
1116	20	-3.182289743025951
1116	21	-2.2972515623785412
1116	22	-2.097639539659081
1116	23	-2.3737483638690513
1116	24	-2.921723427387363
1116	25	-2.37696377816426
1201	1	0.7421758464083297
1201	2	0.7641253847952569
1201	3	0.3505689028550272
1201	4	0.8049782783035653
1201	5	1.022185646494954
1201	6	1.0848082258395664
1201	7	1.3496485758290575
1201	8	1.2101871630728809
1201	9	2.022918329077747
1201	10	1.6451622920470612
1201	11	1.6976409623586548
1201	12	1.6981733925602285
1201	13	1.6841957829325196
1201	14	1.650389029611972
1201	15	1.6865771240188252
1201	16	1.5365523255777305
1201	17	1.5906511516606514
1201	18	1.501263762759109
1201	19	1.6819246671525647
1201	20	1.776005577627231
1201	21	1.825395912710313
1201	22	1.8791766198444932
1201	23	2.2017981551853723
1201	24	2.098043053409434
1201	25	2.199958195998178
1202	1	0.9719615999251623
1202	2	0.9880265319826904
1202	3	0.989648896290948
1202	4	1.0984254667160798
1202	5	1.2226156724511839
1202	6	1.2403802068311762
1202	7	1.271539997275923
1202	8	1.2270261310586008
1202	9	1.97025909746921
1202	10	1.6753555053602085
1202	11	1.6668132901420734
1202	12	1.714695559782676
1202	13	1.7055916233856507
1202	14	1.7801678555234801
1202	15	1.7187315012451094
1202	16	1.6887869669995652
1202	17	1.6488287181883834
1202	18	1.7679296692182618
1202	19	1.6081501288114453
1202	20	1.8406102985844939
1202	21	2.0051969361096695
1202	22	1.8593658120599912
1202	23	2.0905124345060813
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2211	9	1.0334692573416433
2211	10	0.4561871868610652
2211	11	0.7264942035575359
2211	12	0.6969302852888859
2211	13	0.6124042962265861
2211	14	0.5603828566128186
2211	15	0.48200107362210787
2211	16	0.5883155097291208
2211	17	0.45368194791628724
2211	18	0.4158882749424464
2211	19	0.09857888459730901
2211	20	0.652234732695419
2211	21	0.8816406466043674
2211	22	0.7286077612980577
2211	23	1.063867780762692
2211	24	1.1631088975993933
2211	25	1.317666468361523
2212	1	0.0020943735246206074
2212	2	0.0025544141896354233
2212	3	0.0571211277678465
2212	4	0.195288954411879
2212	5	-0.16750445485812548
2212	6	-0.06898808857089733
2212	7	-0.026657823396526226
2212	8	-0.07007152875536349
2212	9	0.3536217161474653
2212	10	0.004250245325017943
2212	11	0.12678794777323077
2212	12	-0.0015551720051973916
2212	13	-0.22647833947624463
2212	14	-0.2488591145158452
2212	15	0.013350147967592818
2212	16	0.09979957055455202
2212	17	-0.17908265654384792
2212	18	-0.14410343352496824
2212	19	0.033646427240654475
2212	20	0.2855341698263345
2212	21	0.3464586939854897
2212	22	-0.022173923554539954
2212	23	0.3408696465633696
2212	24	0.532108158195598
2212	25	0.5165295320951628
2213	1	-0.05709063283722671
2213	2	-0.04520055554926472
2213	3	-0.10536091150627414
2213	4	-0.09671930873150103
2213	5	-0.06748752218726395
2213	6	0.21533854748795278
2213	7	0.10769856578398418
2213	8	0.12876834139524362
2213	9	0.7926578117638385
2213	10	0.13423219993578073
2213	11	0.32747686558204236
2213	12	0.31355699484450383
2213	13	-0.04823053225712215
2213	14	0.07674260003802402
2213	15	0.06578530793295556
2213	16	-0.20995413165298515
2213	17	-0.20918217226945757
2213	18	-0.32521721623525934
2213	19	-0.2530449772518253
2213	20	0.2603006199160802
2213	21	-0.12823857404901418
2213	22	0.06263843826276627
2213	23	-0.2609823038384178
2213	24	-0.01631782910006052
2213	25	0.16241513497081428
2214	1	-0.8776113383040496
2214	2	-0.6679021692440337
2214	3	-0.7440429886838835
2214	4	-0.4833832955931854
2214	5	-0.7960828268613724
2214	6	-0.5732040491047634
2214	7	-0.6392904014119338
2214	8	-0.5393548929220486
2214	9	-10.63943333688583
2214	10	-3.715174515209352
2214	11	-1.682707624730174
2214	12	-1.0479452898553383
2214	13	-0.8130961153954104
2214	14	-0.8649895536828396
2214	15	-0.8633706338222638
2214	16	-0.9811219099390129
2214	17	-0.9513734781292982
2214	18	-1.2602768187721836
2214	19	-1.2846716731437695
2214	20	-1.0470934726259387
2214	21	-1.5858397021443906
2214	22	-1.3462114730850274
2214	23	-1.7222491491425238
2214	24	-2.0580562499220285
2214	25	-9.775939871693403
2215	1	-0.7460875962991231
2215	2	-0.4764501512893702
2215	3	-0.39415194279293075
2215	4	-0.6133730682174061
2215	5	-0.6046803065196329
2215	6	-0.5960350970395467
2215	7	-0.7078251889644349
2215	8	-0.7619975759556823
2215	9	-0.007381696446351782
2215	10	-0.1811281027041005
2215	11	-0.0666752200493832
2215	12	-0.0792673701833877
2215	13	-0.23837166047874092
2215	14	-0.45103202135619824
2215	15	-0.9724589891617939
2215	16	-0.7495986976133437
2215	17	-0.5724785205570626
2215	18	-0.458309188201909
2215	19	-0.4678472701265335
2215	20	-0.3552748800987757
2215	21	-0.40393857737049643
2215	22	-0.5096615863861587
2215	23	-0.11991351934521788
2215	24	0.06867814141212847
2215	25	0.141997606611433
2216	1	-0.9208050010583335
2216	2	-0.9000078799696674
2216	3	-0.8459002691942352
2216	4	-0.7474347780592652
2216	5	-1.663967491498191
2216	6	-2.20509265377148
2216	7	-1.5547482292648098
2216	8	-2.5848750993021525
2216	9	-1.145939734418839
2216	10	-1.6616544558121937
2216	11	-1.1732060801157544
2216	12	-2.4112293196673207
2216	13	-2.041734316822552
2216	14	-2.279654576435366
2216	15	-2.013901654588725
2216	16	-3.006184613951106
2216	17	-3.3723720720241275
2216	18	-2.7706395651844176
2216	19	-4.292175300333355
2216	20	-3.124342460837891
2216	21	-3.216270989914033
2216	22	-3.010589010606463
2216	23	-2.662498417223947
2216	24	-2.7392027253433255
2216	25	-2.719100289059501
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92180.0
3	6.0
4	1389.0
5	13367.0
6	17377.0
7	14071.0
8	11187.0
9	9343.0
10	8233.0
11	7604.0
12	6898.0
13	6392.0
14	6295.0
15	6532.0
16	7050.0
17	7625.0
18	8376.0
19	10161.0
20	13406.0
21	17497.0
22	22109.0
23	26478.0
24	31353.0
25	36205.0
26	40479.0
27	45082.0
28	50899.0
29	59930.0
30	72663.0
31	90219.0
32	111483.0
33	137624.0
34	169209.0
35	210655.0
36	272022.0
37	389429.0
38	908967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.287900135861015	18.331675758659397	11.616371779425409	25.76405232605418
2	17.41708583396519	20.12462254533283	36.66415285345013	25.79413876725185
3	20.865652785757366	22.993931885680386	28.38567205242002	27.75474327614223
4	12.86229844244369	15.475483804469143	35.63115439187201	36.03106336121516
5	13.38787318073382	37.714668560351505	34.32689405629539	14.570564202619288
6	34.14605354026406	35.77906901584299	16.391356264904896	13.683521178988048
7	29.82201686098752	31.08966998286275	19.90687296692888	19.18144018922085
8	27.51628345027113	35.277355396871144	18.623340309565037	18.583020843292694
9	26.19369178539698	14.836312607548038	18.836206441874463	40.13378916518052
10	15.301445892189324	29.661037135847167	32.24261315493063	22.794903817032875
11	36.80429224098563	21.97058016849639	21.191970049321164	20.033157541196818
12	22.956128781618876	25.08245222703508	29.773018671708655	22.188400319637392
13	29.41587673936515	20.720938918714083	25.192246069526302	24.670938272394462
14	21.573234527516654	20.461124805163085	26.920083357202486	31.04555731011778
15	24.42561952264286	28.841795590015245	22.75927149321575	23.973313394126144
16	23.960683818254	27.94404455371276	24.952739413101245	23.142532214932
17	22.661539733336415	27.530039880810026	26.381446697390622	23.426973688462937
18	22.408357975785304	26.92905151168345	27.744531825165375	22.91805868736587
19	23.67886199606576	26.294499207640403	27.437858118300753	22.58878067799309
20	23.418042346114646	26.00507042943516	27.86208459152833	22.714802632921867
21	23.936255261995854	25.388555911703932	27.57687259693401	23.098316229366205
22	23.253520412832255	25.771942769839352	27.64926948435771	23.325267332970686
23	22.893884046513115	25.52968847697511	27.869087801635484	23.70733967487629
24	22.828868277157557	25.833621917226658	28.064369742982308	23.273140062633477
25	23.147266924386308	25.610200004562817	27.855139450207933	23.387393620842943
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	384.0
1	384.0
2	806.0
3	1228.0
4	1228.0
5	1228.0
6	3267.5
7	5307.0
8	5307.0
9	5307.0
10	7231.0
11	9155.0
12	9155.0
13	9155.0
14	10662.0
15	12169.0
16	12169.0
17	12169.0
18	20239.5
19	28310.0
20	28310.0
21	28310.0
22	43106.5
23	57903.0
24	57903.0
25	57903.0
26	81031.0
27	104159.0
28	104159.0
29	104159.0
30	131528.5
31	158898.0
32	158898.0
33	158898.0
34	187289.0
35	215680.0
36	215680.0
37	215680.0
38	243071.5
39	270463.0
40	270463.0
41	270463.0
42	299032.5
43	327602.0
44	327602.0
45	327602.0
46	352710.0
47	377818.0
48	377818.0
49	377818.0
50	385939.0
51	394060.0
52	394060.0
53	394060.0
54	369565.5
55	345071.0
56	345071.0
57	345071.0
58	306036.5
59	267002.0
60	267002.0
61	267002.0
62	223411.0
63	179820.0
64	179820.0
65	179820.0
66	140363.5
67	100907.0
68	100907.0
69	100907.0
70	75751.0
71	50595.0
72	50595.0
73	50595.0
74	35043.5
75	19492.0
76	19492.0
77	19492.0
78	14172.0
79	8852.0
80	8852.0
81	8852.0
82	6057.0
83	3262.0
84	3262.0
85	3262.0
86	2207.0
87	1152.0
88	1152.0
89	1152.0
90	811.0
91	470.0
92	470.0
93	470.0
94	251.5
95	33.0
96	33.0
97	33.0
98	18.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025920174706059434
2	0.0012926071375725177
3	6.803195460907988E-5
4	0.0042860131403720325
5	0.03075044348330411
6	0.01105519262397548
7	0.019865330745851326
8	0.026124270569886678
9	0.03374384948610362
10	0.038267974467607435
11	0.037315527103080316
12	0.007279419143171547
13	0.0024831663432314157
14	0.005340508436812772
15	0.006565083619776209
16	0.008231866507698665
17	0.009728569509098423
18	0.014388758399820395
19	0.01608955726504739
20	0.013572374944511436
21	0.010919128714757323
22	0.010068729282143824
23	0.008061786621175966
24	0.005204444527594611
25	0.10245612364127431
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2939795.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.61870331912547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5368184002918	45.82808342353574
2	12.331806566874148	14.210854079311297
3	3.4851354495824953	6.024269564893822
4	1.4375032781862158	3.3130829962432746
5	0.7810633190440064	2.250192782672402
6	0.4709028286046305	1.627968622410432
7	0.32348479128651364	1.30471419521708
8	0.23668376518647655	1.090992931738652
9	0.17415674193079048	0.9031217079902145
>10	1.0679003608680788	11.62920717777357
>50	0.1003541471622066	3.9940063258454193
>100	0.051587887870445555	5.146036884121055
>500	0.0016561128896832643	0.5937360185195999
>1k	7.097626668510831E-4	0.7605219255443186
>5k	1.7744066671277078E-4	0.8565925165153455
>10k+	5.914688890425693E-5	0.4666188476677187
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13692	0.46574676125376085	No Hit
GGTATCAACGCAGAGTACTTTTTTT	9986	0.3396835493631359	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8891	0.30243605421466463	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	6258	0.21287198597181095	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3904	0.13279837539692393	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3467	0.11793339331483997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.401597730453994E-5
2	0.0	0.0	0.0	0.0	3.401597730453994E-5
3	0.0	0.0	0.0	0.0	3.401597730453994E-5
4	0.0	0.0	0.0	3.401597730453994E-5	3.401597730453994E-5
5	0.0	0.0	0.0	3.401597730453994E-5	3.401597730453994E-5
6	0.0	0.0	0.0	3.401597730453994E-5	3.401597730453994E-5
7	0.0	0.0	0.0	6.803195460907988E-5	3.401597730453994E-5
8	0.0	0.0	0.0	1.3606390921815976E-4	3.401597730453994E-5
9	0.0	0.0	0.0	1.3606390921815976E-4	3.401597730453994E-5
10	0.0	0.0	0.0	1.3606390921815976E-4	6.803195460907988E-5
11	0.0	0.0	0.0	1.3606390921815976E-4	6.803195460907988E-5
12	0.0	0.0	0.0	1.3606390921815976E-4	1.0204793191361982E-4
13	0.0	0.0	0.0	1.3606390921815976E-4	1.0204793191361982E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCGC	80	1.2878627E-7	14.251698	8
GGTATCA	2940	0.0	13.1194935	1
TATAACG	185	0.0	12.838275	2
TAACGCA	205	0.0	12.514538	4
CGCGCCA	115	5.329639E-9	12.3917265	10
CGCGGTT	70	1.0943159E-4	12.210961	18
TTTACGG	110	3.8329745E-8	12.087618	18
GTTCGCG	230	0.0	11.975408	16
TGGTCGG	135	3.7107384E-10	11.963371	10
TGGTATC	930	0.0	11.95202	2
TCGCGCA	240	0.0	11.871768	18
ATAACGC	210	0.0	11.763308	3
TGGACCG	130	2.6029738E-9	11.696687	5
TTCGCGC	245	0.0	11.6296835	17
CTACACG	205	0.0	11.587535	4
GTGGTAT	1080	0.0	11.4355755	1
CGTTCGC	255	0.0	11.173808	15
TCGTTCG	240	0.0	11.080882	14
TCGCGGT	95	1.3650822E-5	10.997193	17
CTTACAC	495	0.0	10.940698	3
>>END_MODULE