FastQCFastQC Report
Thu 26 May 2016
SRR1364294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388691
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67480.2824978199356886No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN64500.270022367899406No Hit
TATCAACGCAGAGTACTTTTTTTTT39500.1653625353802564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA32470.13593219047587152No Hit
GGTATCAACGCAGAGTACTTTTTTT30450.1274756760083242No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27070.1133256666517352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG500.001495397413.3053734
GGTATCA16200.012.1896421
GCGACGT658.108316E-411.678122511
GTATCAA41450.011.4979881
TGTACCG1502.1209416E-910.7710165
GTCATAC803.7948796E-410.6805881
GTACCGT1002.400281E-510.4498276
TCGCGCA1752.3283064E-1010.32301118
TAACGCA1303.8223516E-710.2349014
GGTCTAC2750.010.0117031
CACTGCG951.6452798E-49.9996247
CGCGTCC2002.5465852E-119.98343818
TGGTATC6000.09.968552
GTGGTAT6250.09.87361051
CGACATG1356.370028E-79.86039315
CTAGGAC3900.09.73728753
TTACACT2650.09.6831554
AACGCAT3250.09.6459946
TTGCGCG2106.548362E-119.50803718
GTGCTAT3300.09.4938561
Produced by FastQC (version 0.11.5)