FastQCFastQC Report
Thu 26 May 2016
SRR1364294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388691
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT147650.6181209708580976No Hit
TATCAACGCAGAGTACTTTTTTTTT110410.4622196843375724No Hit
GGTATCAACGCAGAGTACTTTTTTT109270.45744719597469913No Hit
GTACTTTTTTTTTTTTTTTTTTTTT70650.29576868669911677No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50670.21212454854981241No Hit
GAGTACTTTTTTTTTTTTTTTTTTT45960.19240663610320463No Hit
GTGGTATCAACGCAGAGTACATGGG29260.12249386798041272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG250.00602789719.0027878
TATAACG2050.016.6829262
TAACGCA2100.015.8366494
ATAACGC2250.014.7793253
GGTATCA32400.012.7562321
TACGACA604.0862284E-412.669324
AATCGTA550.003076020412.08736518
TTGCGCG1900.011.99648218
GTGGTAT10350.011.93386651
TGGTATC10100.011.7574252
TGCTCGA1701.8189894E-1211.7367710
CGACCTA658.0459524E-411.6888818
TGCGCGC2000.011.4064519
AGCACCG1251.8226274E-811.4045395
ACTTACC752.0718794E-411.4016728
GTTGCCG752.08036E-411.39665913
GTATAAC3700.011.29871751
GCAACCG855.3413252E-511.17319513
TGAGTCG855.343604E-511.17272712
TCTATAC2050.011.1231163
Produced by FastQC (version 0.11.5)