##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364294_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2388691 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.726266813078794 31.0 30.0 34.0 26.0 34.0 2 29.781691729905628 31.0 30.0 34.0 26.0 34.0 3 29.81624496429216 31.0 30.0 34.0 26.0 34.0 4 33.18845802994192 35.0 33.0 37.0 27.0 37.0 5 32.76479419062574 35.0 33.0 37.0 26.0 37.0 6 32.66898188170843 35.0 33.0 37.0 25.0 37.0 7 32.41946530547484 35.0 33.0 37.0 23.0 37.0 8 32.31143751954522 35.0 32.0 37.0 23.0 37.0 9 33.12172692072771 37.0 32.0 39.0 17.0 39.0 10 33.211957092817784 37.0 32.0 39.0 18.0 39.0 11 33.15616712249513 37.0 32.0 39.0 17.0 39.0 12 33.118173928733356 37.0 32.0 39.0 17.0 39.0 13 33.15368626582509 37.0 33.0 39.0 17.0 39.0 14 34.09989404238556 38.0 33.0 41.0 17.0 41.0 15 34.12090052669014 38.0 33.0 41.0 17.0 41.0 16 34.05177940554052 38.0 33.0 41.0 17.0 41.0 17 34.00494287457021 38.0 33.0 41.0 17.0 41.0 18 33.94509252138514 38.0 33.0 41.0 17.0 41.0 19 33.844340687012256 39.0 33.0 41.0 18.0 41.0 20 33.775783473040256 39.0 33.0 41.0 16.0 41.0 21 33.714943875118216 39.0 33.0 41.0 15.0 41.0 22 33.65232129228937 39.0 33.0 41.0 10.0 41.0 23 33.40223578520621 38.0 33.0 41.0 8.0 41.0 24 33.31771669085704 38.0 33.0 41.0 2.0 41.0 25 33.144731570554754 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25331655614354887 1101 2 0.4439095271997111 1101 3 -1.5416745253264779 1101 4 -0.46841300171629996 1101 5 0.3560229996168758 1101 6 0.6794807745670752 1101 7 0.8386090318951318 1101 8 0.8830092352521319 1101 9 1.765171409449998 1101 10 1.3514373089320841 1101 11 1.3134485062395953 1101 12 1.4279098919107938 1101 13 1.212232819692666 1101 14 1.3970599493426263 1101 15 1.225301619893301 1101 16 1.2661329948160045 1101 17 1.3042550449725354 1101 18 1.295739237639772 1101 19 1.3840547288277776 1101 20 1.537003082055584 1101 21 1.4670829639388785 1101 22 1.6313101600783426 1101 23 1.8773614752053405 1101 24 1.936218259653593 1101 25 1.9979669703388367 1102 1 0.6182511247789435 1102 2 0.6503829942193349 1102 3 0.6014271346937541 1102 4 0.5922607363326122 1102 5 0.764019524476204 1102 6 0.9027446314924603 1102 7 0.9281686134031872 1102 8 1.0284296133655744 1102 9 1.6215046498179007 1102 10 1.2676162030113147 1102 11 1.254487071232127 1102 12 1.3425047998272106 1102 13 1.1098200389764443 1102 14 1.2048474112470586 1102 15 1.3420262055711447 1102 16 1.4040907118436223 1102 17 1.2667747705342194 1102 18 1.2897951409127515 1102 19 1.2304347041286547 1102 20 1.5946648221007464 1102 21 1.6759804522059767 1102 22 1.6334599970428414 1102 23 1.9806474116481283 1102 24 1.9507015407632977 1102 25 1.8310709631448958 1103 1 -2.7623385692129254 1103 2 -2.7515949604396006 1103 3 -2.542314866841952 1103 4 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0.4590490119373456 1107 15 0.3253682340205728 1107 16 0.16814445949501078 1107 17 -0.07241238769785241 1107 18 0.037877149325652226 1107 19 0.33674424778214274 1107 20 0.1836677937679667 1107 21 0.2776560005139075 1107 22 0.4351631352978913 1107 23 0.4854385614963661 1107 24 0.7767401619556935 1107 25 0.40979897867371307 1108 1 -0.10737065869269458 1108 2 0.003198157315992489 1108 3 0.06947834802073061 1108 4 -0.0795135764834427 1108 5 0.17642981935171775 1108 6 0.12037935612613637 1108 7 -0.0752066437709118 1108 8 0.15606012177052975 1108 9 0.42380693949610304 1108 10 0.1679070800654472 1108 11 0.26992344401290325 1108 12 0.3770569940234836 1108 13 0.08622873005864307 1108 14 0.07199065372403624 1108 15 -0.1546163862779295 1108 16 -0.7582368503974379 1108 17 -0.7856143177721364 1108 18 -0.6475340442800288 1108 19 -0.5276789173775001 1108 20 -0.014265905633564557 1108 21 -0.32571451584427535 1108 22 -0.19348600476907762 1108 23 -0.5351204429916336 1108 24 -0.2109368700037848 1108 25 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0.7638256648521491 2210 22 0.9528200552018475 2210 23 1.1098605413395504 2210 24 1.13770747622209 2210 25 1.2828707030217217 2211 1 -0.07169883017824574 2211 2 0.042105303434571084 2211 3 0.04070160971064851 2211 4 0.06800408866599383 2211 5 -0.7767900393270892 2211 6 0.010063236459721736 2211 7 0.27072376854217595 2211 8 0.27088021468788526 2211 9 0.7693542571166176 2211 10 0.15724779273214295 2211 11 0.5186260204322082 2211 12 0.5109183878657078 2211 13 0.33631319107450963 2211 14 0.29932305747431087 2211 15 0.3263681117984021 2211 16 0.36017155598187145 2211 17 0.32370362625221816 2211 18 0.19704724407294805 2211 19 0.06330781251719486 2211 20 0.4061998687152695 2211 21 0.6354913622329192 2211 22 0.4485673909732668 2211 23 1.0622320880746656 2211 24 1.0759500709922634 2211 25 1.0228255176265861 2212 1 -0.04941728839495241 2212 2 -0.068082446819826 2212 3 -0.001349489030477713 2212 4 0.10187312375902735 2212 5 -0.22976022932584783 2212 6 -0.06926636281614407 2212 7 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2213 18 -0.20164295282127398 2213 19 -0.1568762533427872 2213 20 0.36253353720410075 2213 21 0.034443712366403645 2213 22 0.21676363800213494 2213 23 -0.07098490392397849 2213 24 0.08998807839650169 2213 25 0.3581755625066023 2214 1 -0.8296057740061364 2214 2 -0.6686166796706878 2214 3 -0.7311473541515952 2214 4 -0.4833093389845047 2214 5 -0.8279741508092044 2214 6 -0.6860125646893245 2214 7 -0.5142202266197309 2214 8 -0.5065795268084479 2214 9 -10.654618773063586 2214 10 -3.7560634088788376 2214 11 -1.5765246232534977 2214 12 -1.0085443388396875 2214 13 -0.7684927381683124 2214 14 -0.8309707025380391 2214 15 -0.8553719305349503 2214 16 -1.1073080011263343 2214 17 -1.002218796914029 2214 18 -1.3166461157669858 2214 19 -1.3463847881531876 2214 20 -1.0151221800131225 2214 21 -1.6005762681930484 2214 22 -1.525137678091859 2214 23 -1.7811940183506145 2214 24 -2.182857904148154 2214 25 -9.983672780675576 2215 1 -0.3085953924235909 2215 2 -0.10821463877870485 2215 3 0.03426073499737825 2215 4 -0.10648205825612678 2215 5 -0.045387226852476203 2215 6 -0.023996099150657813 2215 7 -0.05437639659703564 2215 8 -0.21094086245032528 2215 9 0.7743027423920807 2215 10 0.66670728323124 2215 11 0.6824022196112765 2215 12 0.669781275700295 2215 13 0.5967422101846509 2215 14 0.2871164692530499 2215 15 -0.12999273022299462 2215 16 0.23874202708971382 2215 17 0.30285871826153254 2215 18 0.3432167995615387 2215 19 0.27213475506266604 2215 20 0.4940981509178428 2215 21 0.3238114573465225 2215 22 0.23289538205156646 2215 23 0.5361559663399476 2215 24 0.7551792476651613 2215 25 0.7232519586864257 2216 1 -0.576251933203519 2216 2 -0.5818306819968022 2216 3 -0.5669936676984833 2216 4 -0.35477723537467654 2216 5 -1.2210656518037837 2216 6 -1.92412194631304 2216 7 -1.0547570802811812 2216 8 -1.9315822442292898 2216 9 -0.6140949630221755 2216 10 -1.0525617403824405 2216 11 -0.5284697530918621 2216 12 -1.7131060732063652 2216 13 -1.4579850152582807 2216 14 -1.6276415902650854 2216 15 -1.1662793860507676 2216 16 -2.1874042521725414 2216 17 -2.498249188548723 2216 18 -2.0805188209050804 2216 19 -3.6229057285981945 2216 20 -2.3989850480877735 2216 21 -2.4443935922944 2216 22 -2.1968917188966515 2216 23 -1.7606084685076802 2216 24 -1.977691000160064 2216 25 -2.066579660098178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78598.0 3 1.0 4 1152.0 5 11454.0 6 14852.0 7 11726.0 8 9370.0 9 8029.0 10 7149.0 11 6260.0 12 5782.0 13 5447.0 14 5458.0 15 5456.0 16 5722.0 17 6236.0 18 7006.0 19 8248.0 20 10612.0 21 14257.0 22 17665.0 23 21617.0 24 25351.0 25 29062.0 26 32435.0 27 35942.0 28 40250.0 29 47071.0 30 57422.0 31 71838.0 32 87908.0 33 108664.0 34 135849.0 35 169188.0 36 220727.0 37 320458.0 38 744429.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.71380276806611 17.74368818364663 12.413081794506832 25.129427253780435 2 17.335821198571082 20.046670498660127 37.40869884843433 25.208809454334457 3 20.36049900175368 23.75868101707673 28.13932663481935 27.74149334635024 4 12.956923432460405 15.83691425547521 34.77805260355089 36.428109708513496 5 13.734472449603905 37.6368181184313 33.685702237039315 14.943007194925487 6 34.034467180563524 35.5595777454272 16.52856264211819 13.877392431891082 7 30.203306057812046 30.92430268207897 19.956980548709456 18.915410711399527 8 27.440187200014744 35.13216310990547 18.558613487077654 18.869036203002135 9 26.632360257499084 15.22652615779943 18.323274251934343 39.81783933276714 10 15.463582052672686 29.508012723195122 31.493090819238912 23.535314404893278 11 36.558140976324985 22.294413539543452 21.30651300721043 19.84093247692114 12 23.949823468606386 25.083409147143826 29.088848482767833 21.87791890148196 13 29.586859067213478 20.90706464129199 25.13354946856522 24.372526822929313 14 22.388873910007252 20.302627370268464 26.821734375863493 30.486764343860788 15 24.69496061198789 28.347269298786205 22.78132619704724 24.176443892178668 16 24.242117215184784 27.652547879502414 24.56060215298665 23.54473275232615 17 23.113650912741793 27.163284505558593 26.16498559952572 23.558078982173893 18 22.70638322103701 26.321261269409007 27.921169824529667 23.05118568502431 19 24.126456951853857 25.58632930409234 27.432502990632212 22.85471075342159 20 23.87882660217573 25.36835115180129 27.91002643670557 22.84279580931741 21 24.5578108533701 24.732990816868693 27.678141149664565 23.031057180096642 22 24.134165738926043 24.706762088226565 27.65754249987125 23.50152967297614 23 23.39163976314574 25.046881417541666 28.058638301542448 23.50284051777014 24 23.75870772886689 25.29173691532774 27.857363852966188 23.09219150283918 25 23.456765289864364 24.9453327265245 27.946102971435565 23.65179901217557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1088.0 1 1088.0 2 1389.0 3 1690.0 4 1690.0 5 1690.0 6 5246.5 7 8803.0 8 8803.0 9 8803.0 10 8951.0 11 9099.0 12 9099.0 13 9099.0 14 9335.0 15 9571.0 16 9571.0 17 9571.0 18 16241.0 19 22911.0 20 22911.0 21 22911.0 22 34664.0 23 46417.0 24 46417.0 25 46417.0 26 66148.0 27 85879.0 28 85879.0 29 85879.0 30 105664.5 31 125450.0 32 125450.0 33 125450.0 34 146419.0 35 167388.0 36 167388.0 37 167388.0 38 187227.5 39 207067.0 40 207067.0 41 207067.0 42 230066.0 43 253065.0 44 253065.0 45 253065.0 46 275429.0 47 297793.0 48 297793.0 49 297793.0 50 304846.0 51 311899.0 52 311899.0 53 311899.0 54 299035.5 55 286172.0 56 286172.0 57 286172.0 58 256669.0 59 227166.0 60 227166.0 61 227166.0 62 192218.5 63 157271.0 64 157271.0 65 157271.0 66 124277.5 67 91284.0 68 91284.0 69 91284.0 70 68886.5 71 46489.0 72 46489.0 73 46489.0 74 32328.0 75 18167.0 76 18167.0 77 18167.0 78 13541.0 79 8915.0 80 8915.0 81 8915.0 82 6138.5 83 3362.0 84 3362.0 85 3362.0 86 2272.0 87 1182.0 88 1182.0 89 1182.0 90 848.0 91 514.0 92 514.0 93 514.0 94 279.5 95 45.0 96 45.0 97 45.0 98 24.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028048835115132092 2 0.0012559179902297954 3 8.37278660153197E-5 4 0.004479440831819603 5 0.03173286121980616 6 0.011470717644098797 7 0.019634184580592465 8 0.02750460398603252 9 0.03344928247312021 10 0.038766001965093014 11 0.03767753970689386 12 0.006865685013256214 13 0.00263742777948257 14 0.005986542420095358 15 0.0061539981521259975 16 0.00816346693649367 17 0.009544976725746444 18 0.015364063413811163 19 0.016745573203063937 20 0.014443056887642646 21 0.012140540572221355 22 0.011386989778083478 23 0.009126337395669846 24 0.006363317817164296 25 0.10202240473966705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2388691.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.72504489360328 #Duplication Level Percentage of deduplicated Percentage of total 1 76.96134522267306 38.269063462695186 2 12.584700903648175 12.515496348129505 3 4.1231096376945535 6.1506543550683 4 1.9053143915006263 3.789673746151882 5 1.0457650304407426 2.600035654341317 6 0.656695053409412 1.959251460731413 7 0.435872955749446 1.517166160478413 8 0.3224988924082733 1.282901752251098 9 0.23643243296071187 1.0580952008947722 >10 1.4851379457841958 14.219334632299887 >50 0.1544703291766952 5.307319115265551 >100 0.08452689459018067 7.552563786625437 >500 0.00286592936285634 0.9295743423668451 >1k 8.429204008400278E-4 0.8007299231930289 >5k 1.6858408016800556E-4 0.5085037389121561 >10k+ 2.528761202520083E-4 1.5396363205951389 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 14765 0.6181209708580976 No Hit TATCAACGCAGAGTACTTTTTTTTT 11041 0.4622196843375724 No Hit GGTATCAACGCAGAGTACTTTTTTT 10927 0.45744719597469913 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 7065 0.29576868669911677 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5067 0.21212454854981241 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4596 0.19240663610320463 No Hit GTGGTATCAACGCAGAGTACATGGG 2926 0.12249386798041272 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.186393300765984E-5 0.0 0.0 0.0 0.0 8 4.186393300765984E-5 0.0 0.0 0.0 4.186393300765984E-5 9 4.186393300765984E-5 0.0 0.0 4.186393300765984E-5 4.186393300765984E-5 10 4.186393300765984E-5 0.0 0.0 4.186393300765984E-5 4.186393300765984E-5 11 4.186393300765984E-5 0.0 0.0 4.186393300765984E-5 4.186393300765984E-5 12 4.186393300765984E-5 0.0 0.0 4.186393300765984E-5 2.0931966503829922E-4 13 4.186393300765984E-5 0.0 0.0 4.186393300765984E-5 2.5118359804595904E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCG 25 0.006027897 19.002787 8 TATAACG 205 0.0 16.682926 2 TAACGCA 210 0.0 15.836649 4 ATAACGC 225 0.0 14.779325 3 GGTATCA 3240 0.0 12.756232 1 TACGACA 60 4.0862284E-4 12.66932 4 AATCGTA 55 0.0030760204 12.087365 18 TTGCGCG 190 0.0 11.996482 18 GTGGTAT 1035 0.0 11.9338665 1 TGGTATC 1010 0.0 11.757425 2 TGCTCGA 170 1.8189894E-12 11.73677 10 CGACCTA 65 8.0459524E-4 11.68888 18 TGCGCGC 200 0.0 11.40645 19 AGCACCG 125 1.8226274E-8 11.404539 5 ACTTACC 75 2.0718794E-4 11.401672 8 GTTGCCG 75 2.08036E-4 11.396659 13 GTATAAC 370 0.0 11.2987175 1 GCAACCG 85 5.3413252E-5 11.173195 13 TGAGTCG 85 5.343604E-5 11.172727 12 TCTATAC 205 0.0 11.123116 3 >>END_MODULE