FastQCFastQC Report
Thu 26 May 2016
SRR1364293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382092
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA87300.6316511491275545No Hit
GTATCAACGCAGAGTACTTTTTTTT83490.6040842433065238No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49120.355403258249089No Hit
TATCAACGCAGAGTACTTTTTTTTT45120.3264616248411828No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA41270.298605302686073No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN40230.29108047800001735No Hit
GGTATCAACGCAGAGTACTTTTTTT34480.2494768799761521No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28720.20780092786876705No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24290.17574806886951086No Hit
GAACAAAAAAAAAAAAAAAAAAAAA22060.15961310824460312No Hit
AAACAAAAAAAAAAAAAAAAAAAAA16260.11764773980313901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG851.02591E-915.63940052
ATAACGC2300.015.2751273
TATAACG2200.014.6746312
TCTGCGC653.340383E-614.62842618
TAACGCA2350.014.1533224
ACCGAGT551.9638099E-413.8149328
CGCATAT903.648711E-813.7189948
CGCGCCA707.284232E-613.56724910
AAGGCGT852.7023998E-713.4100726
GTTATCA2600.013.1469311
ATCGTAG655.504766E-513.14024613
ATATCGT604.127354E-412.65357211
GAGCGTT550.003091999612.07840911
CTCAACG951.0456388E-611.9941263
GTATTAG1701.8189894E-1211.7291241
AATGCGT658.0923113E-411.6802212
ATGCGTA658.0923113E-411.6802213
GTATCAA42950.011.5399131
TAGACAG1406.7666406E-1011.538875
GGTATCA17050.011.527661
Produced by FastQC (version 0.11.5)