##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364293_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1382092
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.885909186942694	30.0	18.0	31.0	2.0	34.0
2	23.80722195049244	30.0	16.0	31.0	2.0	34.0
3	23.359751738668628	28.0	16.0	31.0	2.0	34.0
4	26.731373888279506	33.0	19.0	35.0	2.0	37.0
5	27.363163233706583	33.0	25.0	35.0	2.0	37.0
6	27.420820755781815	35.0	25.0	36.0	2.0	37.0
7	27.022897173270664	35.0	23.0	37.0	2.0	37.0
8	26.521767726026923	33.0	18.0	36.0	2.0	37.0
9	27.319110449955573	34.0	17.0	39.0	2.0	39.0
10	26.609481134396262	33.0	17.0	38.0	2.0	39.0
11	27.219588855155806	34.0	17.0	39.0	2.0	39.0
12	26.763435429768784	34.0	17.0	38.0	2.0	39.0
13	26.397676131545513	33.0	15.0	38.0	2.0	39.0
14	26.535604720959242	33.0	11.0	39.0	2.0	41.0
15	27.16679569811561	34.0	17.0	39.0	2.0	41.0
16	27.45551670945205	34.0	17.0	39.0	2.0	41.0
17	27.592968485455383	34.0	17.0	39.0	2.0	41.0
18	27.408044471713893	34.0	17.0	40.0	2.0	41.0
19	27.38176040379367	34.0	15.0	40.0	2.0	41.0
20	27.2416561270885	34.0	11.0	40.0	2.0	41.0
21	27.1459121389893	34.0	10.0	40.0	2.0	41.0
22	27.177859361026616	34.0	10.0	40.0	2.0	41.0
23	27.138270100687944	35.0	2.0	40.0	2.0	41.0
24	26.986903910882923	35.0	2.0	40.0	2.0	41.0
25	26.782531843032157	34.0	2.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7700676213756275
1101	2	0.9146941329318246
1101	3	1.5142521982234634
1101	4	0.9674595306272842
1101	5	0.5351231115371853
1101	6	0.5855965726953265
1101	7	0.8031832960907543
1101	8	1.0356902016741856
1101	9	1.0449343653314322
1101	10	1.353976743417146
1101	11	0.8181043038804781
1101	12	0.8839259283718128
1101	13	0.7596104654844389
1101	14	1.3917545528542234
1101	15	0.7236048251500797
1101	16	0.05975242013381887
1101	17	-0.5125548449237343
1101	18	-5.794386691916159
1101	19	-7.640323242986085
1101	20	-8.5857298277306
1101	21	-10.003605654096123
1101	22	-10.319475831129228
1101	23	-11.494671442654427
1101	24	-11.460788326604302
1101	25	-11.782887863629458
1102	1	1.985643515977987
1102	2	2.0139042148656756
1102	3	2.8426545889346713
1102	4	2.2502393167851764
1102	5	1.717833175335162
1102	6	1.765142127995638
1102	7	1.943463072173163
1102	8	2.261437903455935
1102	9	2.448831160117976
1102	10	-1.1154985655979957
1102	11	1.0884658526035125
1102	12	2.0672590601954326
1102	13	2.611028086299587
1102	14	3.004005537229162
1102	15	2.6430791266166658
1102	16	2.2845768044208974
1102	17	2.146819588330203
1102	18	2.486151903238742
1102	19	2.18674031720688
1102	20	2.6262366249327513
1102	21	2.6485966455326952
1102	22	2.6074353877224077
1102	23	2.5812320261337334
1102	24	2.800560498795594
1102	25	2.873982609958663
1103	1	0.23027801383848967
1103	2	0.18034929737318706
1103	3	-2.5597608646052734
1103	4	-0.283283201006725
1103	5	-3.243248080879436
1103	6	-6.716507351933306
1103	7	-7.67848828358672
1103	8	-2.6243699104580607
1103	9	-4.921391280584988
1103	10	-2.918117355189871
1103	11	-6.889340352497765
1103	12	-1.8722037751135368
1103	13	0.3143529659179407
1103	14	0.8080599687261376
1103	15	0.4509153852333121
1103	16	0.6661335541723119
1103	17	0.4037101108591692
1103	18	1.025456091737965
1103	19	-5.848525081030129
1103	20	-1.989966577051451
1103	21	-7.962076262731461
1103	22	-3.338948693969524
1103	23	-1.5998040939462577
1103	24	-7.142650646701409
1103	25	-3.412799429434905
1104	1	1.5190381856287267
1104	2	1.5488958615684858
1104	3	2.3533381408196803
1104	4	1.7382357863350464
1104	5	1.413900427257996
1104	6	1.4119623587357708
1104	7	1.5372158303373311
1104	8	1.9314562649488103
1104	9	1.9645038369557
1104	10	2.3213548419168184
1104	11	1.7784659314082027
1104	12	1.9377419753385432
1104	13	2.1061307688112514
1104	14	2.5938929253265
1104	15	1.679176009103113
1104	16	0.9635623516406682
1104	17	0.7946063585987702
1104	18	0.7266484515933023
1104	19	1.2131442227673404
1104	20	-8.208874804899551
1104	21	-3.3265936677947536
1104	22	-1.092237572812202
1104	23	-0.06329301799689091
1104	24	0.48085183973620715
1104	25	1.0710443770750402
1105	1	0.890921798398594
1105	2	0.9468029584908564
1105	3	1.703363979129282
1105	4	1.152521500620761
1105	5	1.1041263689208343
1105	6	1.1141241639723347
1105	7	1.3016580099422903
1105	8	1.5515311960466498
1105	9	1.260966859463739
1105	10	2.077623502918197
1105	11	1.4477714550646112
1105	12	1.4847791825043686
1105	13	1.635562038907711
1105	14	2.3397171674027533
1105	15	1.5591470127778955
1105	16	1.0682692303728665
1105	17	0.6665674862974384
1105	18	1.1051122205413932
1105	19	1.1092498153259065
1105	20	0.8940219566989782
1105	21	0.9478450667022607
1105	22	0.8648679623278994
1105	23	0.8161439939461488
1105	24	0.7186001746006987
1105	25	0.9418112006855139
1106	1	0.9630793346970492
1106	2	1.1159047488421727
1106	3	1.777309827089006
1106	4	1.3947575254654794
1106	5	0.6552811759034292
1106	6	0.5623249528946168
1106	7	0.7737242715954231
1106	8	0.7736324080702595
1106	9	1.0632748162077164
1106	10	-1.6366796334366889
1106	11	0.04563546689121978
1106	12	0.542525905561881
1106	13	1.5051631627586524
1106	14	0.37467667383977954
1106	15	1.3208904434892617
1106	16	0.8589109871633305
1106	17	-5.403980845709409
1106	18	-1.6633166105184998
1106	19	-0.16914358944357488
1106	20	0.4677729749339363
1106	21	0.9997968271173647
1106	22	0.9852939786613
1106	23	0.3652540449078252
1106	24	0.6920546777169108
1106	25	0.7623607012457825
1107	1	0.276699125342752
1107	2	0.6459757598037044
1107	3	1.0396216330263002
1107	4	0.6284972831790476
1107	5	0.05190321290787381
1107	6	0.18537711462911233
1107	7	0.21602838669440771
1107	8	0.3763434702817996
1107	9	0.40216968778656437
1107	10	-2.2912896737343473
1107	11	-0.5448002833283709
1107	12	-0.1976309475005671
1107	13	0.5121420563289618
1107	14	1.0129999876735312
1107	15	0.8330135235949321
1107	16	-5.618687986501804
1107	17	-2.5061242706682663
1107	18	-0.9235000895634684
1107	19	-0.035584925113635535
1107	20	0.3431822284638386
1107	21	0.7040786721511445
1107	22	0.3108612614013744
1107	23	0.05124429038903955
1107	24	0.23468210831794067
1107	25	0.5891466732234392
1108	1	-0.4767877483360188
1108	2	-2.949974501493866
1108	3	-0.1556183837791103
1108	4	-0.9001620037202045
1108	5	-1.0513990005241673
1108	6	-0.6822217375546806
1108	7	-0.3061370702151578
1108	8	-0.0696807713574259
1108	9	-0.6031231033231741
1108	10	-3.7533039649761015
1108	11	-1.5377131990837754
1108	12	-5.62159767212265
1108	13	-1.9296501112203224
1108	14	-5.9236986624135675
1108	15	-2.8328282332015426
1108	16	-7.14453502404994
1108	17	-3.4202227982020723
1108	18	-2.2227489109428724
1108	19	-0.8988665967538374
1108	20	-0.8741717662255191
1108	21	-0.34906719449825374
1108	22	-0.3855370334804462
1108	23	-0.2825091782178859
1108	24	-0.032380697753929155
1108	25	-0.42405313015989776
1109	1	-7.907914286404193
1109	2	-5.952294346738057
1109	3	-5.095291632871977
1109	4	-4.940702107454332
1109	5	-2.8548250613622947
1109	6	-1.6797688679583267
1109	7	-1.4466866224079453
1109	8	-1.0463754021464702
1109	9	-5.898245295100363
1109	10	-2.0403017255256373
1109	11	-1.658324845883815
1109	12	-5.81609626562841
1109	13	-7.3816444819281095
1109	14	-7.564759591710917
1109	15	-3.9645559087315263
1109	16	-2.5649901911136084
1109	17	-2.100499803803185
1109	18	-1.5037854400101445
1109	19	-1.2207863577907148
1109	20	-0.8950640488239436
1109	21	-0.9400753284608214
1109	22	-1.079813919732608
1109	23	-1.3461097371688098
1109	24	-0.9986932129833512
1109	25	-0.9497657842982399
1110	1	-2.428398310005843
1110	2	-2.201040080478837
1110	3	-1.5422086452109411
1110	4	-2.2167686423629185
1110	5	-4.951862178714421
1110	6	-4.06907095139962
1110	7	-8.607500179405807
1110	8	-9.267949419601361
1110	9	-10.6599299223762
1110	10	-8.718680192247422
1110	11	-11.068500805906742
1110	12	-6.630004450069531
1110	13	-5.003330432631241
1110	14	-4.552021205383358
1110	15	-9.405690101867403
1110	16	-10.937038290520537
1110	17	-11.751484920029599
1110	18	-8.862800570929792
1110	19	-11.42007377988988
1110	20	-12.67268680667103
1110	21	-13.211821192152593
1110	22	-14.096491495373254
1110	23	-14.741584094170497
1110	24	-14.942731372335158
1110	25	-15.367290353407054
1111	1	-1.057259451929184
1111	2	-0.5402474556873358
1111	3	-2.732218675198599
1111	4	-3.325424808955937
1111	5	-1.3968703809542262
1111	6	-0.9266502420131033
1111	7	-0.4202711199070066
1111	8	-0.13135133643470098
1111	9	-4.982351528913437
1111	10	-4.7668624979956
1111	11	-2.0642696384972368
1111	12	-5.774773097327575
1111	13	-1.7631568890550966
1111	14	-0.5368444057009789
1111	15	-0.2946604207375927
1111	16	-0.3291006314779459
1111	17	-0.32378090579165786
1111	18	-0.16716997037788772
1111	19	-0.12455430011138091
1111	20	-0.04527251702749524
1111	21	0.12875256721191164
1111	22	-0.2510721384020975
1111	23	-0.5006639087121307
1111	24	-0.30790771950730544
1111	25	-0.25573280925484454
1112	1	-0.7554694446045751
1112	2	-0.14452256284825893
1112	3	-2.696532025367599
1112	4	-0.9362608579602565
1112	5	-0.7741318913741537
1112	6	-0.5375562574355293
1112	7	-0.21309876697810637
1112	8	-3.9259812150767566
1112	9	-1.2915524110468581
1112	10	-3.8802361216498156
1112	11	-1.2330862817589434
1112	12	-0.1641755702031631
1112	13	0.5203196070982408
1112	14	0.9881352950931976
1112	15	0.48733256870681885
1112	16	0.22318902949301744
1112	17	-0.12927207700365884
1112	18	0.019710332385887597
1112	19	0.33345322724161264
1112	20	0.27791210785212783
1112	21	0.1520346879099428
1112	22	0.17413147480684543
1112	23	-0.1790298748201593
1112	24	0.027465174667494097
1112	25	0.06298569219169181
1113	1	-2.799644566510242
1113	2	-2.368204540298251
1113	3	-4.581194851142985
1113	4	-3.220566391523395
1113	5	-3.47503608427947
1113	6	-3.5416009676837277
1113	7	-3.10047132937229
1113	8	-9.956240617343127
1113	9	-10.658934068151897
1113	10	-5.8938002332711505
1113	11	-9.472939791951923
1113	12	-8.996948198371594
1113	13	-5.052976860175008
1113	14	-3.5976006253605632
1113	15	-9.12833063466147
1113	16	-5.218782249085287
1113	17	-4.555144905336931
1113	18	-3.7946982881007614
1113	19	-3.6822221565430056
1113	20	-8.089739142467607
1113	21	-5.0041401741468405
1113	22	-4.605299862156251
1113	23	-4.317302772987819
1113	24	-7.9892471685603965
1113	25	-6.134708961011555
1114	1	-4.558961030801495
1114	2	-4.22470814700954
1114	3	-1.2881848713875108
1114	4	-1.2943277240575632
1114	5	-1.1730665390897457
1114	6	-1.219267021006889
1114	7	-0.8726210634271041
1114	8	-0.7935282698581858
1114	9	-1.1192309040538682
1114	10	-3.9106715107720618
1114	11	-1.973630117750492
1114	12	-4.867701772599432
1114	13	-1.9984046567908607
1114	14	-5.677711949302335
1114	15	-2.56357287740299
1114	16	-1.8156596460498626
1114	17	-1.6626798093326052
1114	18	-1.2840215005677038
1114	19	-1.0384218857776943
1114	20	-0.737497784114332
1114	21	-0.5815518086723515
1114	22	-0.7864535906281027
1114	23	-0.9460080872809584
1114	24	-0.9647776967204074
1114	25	-0.936885142885366
1115	1	0.4362287666445681
1115	2	0.547769272189278
1115	3	1.3598193232498161
1115	4	0.2449944726748825
1115	5	0.2555891214172412
1115	6	-0.6158530522929908
1115	7	-2.435605147768335
1115	8	-0.3182167224041521
1115	9	-0.19824859385955662
1115	10	0.5516017056551767
1115	11	-0.24084566341059244
1115	12	0.4434807391924913
1115	13	0.713743852716874
1115	14	1.3395669665993637
1115	15	0.38017149594611155
1115	16	-0.01159977353781727
1115	17	-0.14520270341251518
1115	18	-0.011184085633871632
1115	19	0.2681463051090809
1115	20	0.3391172227063528
1115	21	0.4059220985070091
1115	22	-0.20908286756980132
1115	23	-0.1392940294200251
1115	24	-0.2631146813081955
1115	25	-0.027913389634687036
1116	1	0.2691259450914849
1116	2	0.4766507455733695
1116	3	1.2048872861188258
1116	4	0.5994079475072063
1116	5	-0.20830271682014967
1116	6	-1.2716533311299187
1116	7	-4.725272722776225
1116	8	-1.3704248400572965
1116	9	-1.3580846856328215
1116	10	-0.8223073986204206
1116	11	-2.732542157724886
1116	12	-0.9196450820485147
1116	13	-6.353496062690581
1116	14	-1.7615061894476156
1116	15	-1.5687597357076335
1116	16	-1.011784870581554
1116	17	-0.5263605217431113
1116	18	-0.5070549977351497
1116	19	-0.032502144754026574
1116	20	-0.10885191112665638
1116	21	0.08806979374370627
1116	22	-0.15395155327618326
1116	23	-0.26774585682473173
1116	24	-0.03809275733339135
1116	25	0.014790561446226036
1201	1	2.849005640722428
1201	2	3.043200300320837
1201	3	3.6001821360210293
1201	4	3.4686488594172076
1201	5	2.8797803556024597
1201	6	2.661127863566847
1201	7	3.134014230246546
1201	8	3.043413377003798
1201	9	3.553648509804411
1201	10	3.6760924967381676
1201	11	3.2312748040494057
1201	12	3.6755475651187126
1201	13	3.9811508307397894
1201	14	4.410949009562284
1201	15	4.086303262523774
1201	16	3.4122076648499267
1201	17	3.362638384731966
1201	18	3.320815699177764
1201	19	3.3561268033392864
1201	20	3.5920188628262792
1201	21	3.3235354175434217
1201	22	2.5723460307154546
1201	23	1.9112998667057859
1201	24	2.5230842257682404
1201	25	2.654393179033409
1202	1	1.856468516305025
1202	2	2.039712744119239
1202	3	2.7855814152000526
1202	4	2.322281121993285
1202	5	1.8020103257509597
1202	6	1.7164437698704091
1202	7	2.0205173778525882
1202	8	2.1006916149301276
1202	9	2.3594845319363955
1202	10	-1.025723327842801
1202	11	1.073429890182645
1202	12	1.9796276398048533
1202	13	2.4559606822500335
1202	14	3.019166542838022
1202	15	2.5161929944063886
1202	16	2.2723379619283754
1202	17	1.9122077877929406
1202	18	2.134264982294507
1202	19	2.2334499115206086
1202	20	2.2550037914387246
1202	21	2.5018582720483167
1202	22	2.277502587855377
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2211	14	-4.305033232974658
2211	15	-0.9951201620680585
2211	16	0.13772177892509418
2211	17	0.4537239583413708
2211	18	0.9240409333607822
2211	19	1.168781556529673
2211	20	1.2933854911107439
2211	21	1.2822584879324026
2211	22	1.0639641646395361
2211	23	0.9183578292789534
2211	24	1.0795688603282265
2211	25	1.1352241818798454
2212	1	0.8847399575240686
2212	2	0.987499073704587
2212	3	1.5520952038340532
2212	4	1.3608497158277366
2212	5	0.8166254456832505
2212	6	0.7423125921223317
2212	7	0.980893280896229
2212	8	1.4122007220179675
2212	9	1.1953354301260717
2212	10	1.8530658571483656
2212	11	0.8469483813529273
2212	12	1.1178427000652356
2212	13	1.2534019117912258
2212	14	-3.9548023363121203
2212	15	-0.9220941819053614
2212	16	-0.20056866727592748
2212	17	0.10567034029634925
2212	18	0.46874440110810056
2212	19	0.8268506931026778
2212	20	1.1788025381786937
2212	21	1.092567445704816
2212	22	1.0210217012327547
2212	23	0.9618816380650159
2212	24	1.0824383309535008
2212	25	1.1916772078083682
2213	1	0.5918937512716127
2213	2	0.8407532044625583
2213	3	1.4071292253275907
2213	4	0.862190978062749
2213	5	0.45159373217582655
2213	6	0.17336715654459667
2213	7	0.3067292405053621
2213	8	-4.808380512610125
2213	9	-1.9193270241263463
2213	10	-0.04520956228087769
2213	11	-0.006337609245456122
2213	12	0.5560653581169035
2213	13	-6.831282099718326
2213	14	-1.799529514200799
2213	15	-0.6466854960978488
2213	16	-0.2309672153563156
2213	17	0.1232002913248138
2213	18	0.26180936591594417
2213	19	0.5342867798548028
2213	20	0.6772545574442681
2213	21	0.7155016036399715
2213	22	0.3820303689616651
2213	23	0.3002935287501991
2213	24	0.5514985360493725
2213	25	0.6520787603457983
2214	1	-0.6812235138786633
2214	2	-0.3952235965595925
2214	3	-6.077973435535
2214	4	-2.200126282630471
2214	5	-1.174446247618853
2214	6	-0.9532162126928014
2214	7	-0.26784882483508454
2214	8	-5.25261085327779
2214	9	-1.3542153748669605
2214	10	0.11182590103011947
2214	11	-0.18312581736027056
2214	12	0.16600552213657238
2214	13	-5.393607531681361
2214	14	-1.1656360155601995
2214	15	-0.559909582029892
2214	16	-0.4981833380145062
2214	17	-0.45156051332241276
2214	18	-0.28063295234758456
2214	19	0.21777784345699658
2214	20	0.4529786433763121
2214	21	0.49291064255007555
2214	22	0.4589015897493738
2214	23	0.22194405096862369
2214	24	0.33176009096773384
2214	25	0.5637937613114943
2215	1	0.46717952549800756
2215	2	0.6673424068532654
2215	3	-2.166835762074811
2215	4	-0.4627912911626204
2215	5	0.056690459937733806
2215	6	-0.48094730512977435
2215	7	0.3329762178256601
2215	8	0.7882694068722458
2215	9	0.8148306343413196
2215	10	1.612440859657248
2215	11	0.8780661881775771
2215	12	0.6242032265056778
2215	13	1.0667079163369273
2215	14	1.1142389804152018
2215	15	0.9071434460779031
2215	16	0.666553131285859
2215	17	0.661170901082432
2215	18	0.8969886710144017
2215	19	0.87843658971412
2215	20	1.2392052604599257
2215	21	1.351087821467619
2215	22	1.2051305411000293
2215	23	1.0561426729461516
2215	24	0.8502933663468504
2215	25	0.87710471787803
2216	1	0.28139452177724067
2216	2	-0.14705615420714935
2216	3	1.0622572508507524
2216	4	-0.33700124657070063
2216	5	-0.5796018335180371
2216	6	-0.7834448797312845
2216	7	-1.4473962025684166
2216	8	-6.140448613148553
2216	9	-2.1495747225216313
2216	10	-1.1973784761454844
2216	11	-1.6513367237587175
2216	12	-0.5408095345013102
2216	13	0.03365870483228406
2216	14	0.903303779043334
2216	15	0.3382691590513254
2216	16	-0.22821295510780004
2216	17	-0.1852187051411711
2216	18	-0.19114221249720842
2216	19	0.20869462711759112
2216	20	0.24890893743173947
2216	21	0.22915263728053858
2216	22	-0.14938590760624137
2216	23	-0.4782766348401708
2216	24	-0.4160450708685275
2216	25	-0.3298467371788014
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	207558.0
3	6.0
4	1337.0
5	8533.0
6	12930.0
7	11429.0
8	9598.0
9	7949.0
10	7102.0
11	6542.0
12	6157.0
13	6001.0
14	6051.0
15	6183.0
16	6112.0
17	6442.0
18	7273.0
19	9485.0
20	12935.0
21	16311.0
22	19929.0
23	22331.0
24	24961.0
25	27106.0
26	28865.0
27	30960.0
28	33086.0
29	37932.0
30	42069.0
31	47358.0
32	56509.0
33	69402.0
34	83919.0
35	101006.0
36	120625.0
37	134320.0
38	145780.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.599141733741405	18.721386586503726	11.6023511557913	22.077120523963565
2	14.462404240641904	23.227535126892494	39.875322223432455	22.434738409033148
3	16.54650083588359	31.21600364854835	29.305744575471355	22.931750940096705
4	10.167151553356176	18.15623470355582	38.359814489082424	33.31679925400558
5	11.252276906718727	42.11681875096233	33.03795280302832	13.59295153929062
6	27.729385216481813	39.651617108345064	18.856138726041326	13.7628589491318
7	26.433458309590264	35.76566407398401	21.531945165306205	16.26893245111952
8	25.685494817866783	35.376497693845465	21.376535440158303	17.561472048129453
9	27.60330014604729	17.77624858816117	19.858946775490768	34.76150449030078
10	13.394373412685438	33.26996791762496	32.222839889691855	21.112818779997745
11	32.145016640837376	27.23783875990375	22.560475016967484	18.05666958229139
12	21.734803573930257	30.636456614667225	29.434040604133294	18.194699207269228
13	26.752013982288425	25.477610846144742	26.73241671342188	21.03795845814495
14	20.62793614661425	24.788844239781195	29.011015812365347	25.57220380123921
15	22.37061091058477	32.25649113817108	24.197049855454093	21.17584809579006
16	20.867308795029434	32.109877985933174	25.877216540491105	21.145596678546283
17	19.936667058596395	31.63221314009746	26.96708806370745	21.46403173759869
18	19.557386532304715	30.133019202755758	29.950550563192284	20.359043701747243
19	20.926159532108056	29.810474148505467	29.81301451492792	19.45035180445856
20	21.239745752850826	29.374127298114967	29.586266581335817	19.799860367698393
21	22.040066513809744	28.672866589875294	29.352738759100827	19.934328137214127
22	21.24823712237401	29.086047299921102	29.076248494794655	20.589467082910232
23	21.03617887447534	29.186822693975074	29.16773419519478	20.609264236354807
24	20.633383729766727	29.193615935628053	29.545640950453024	20.627359384152196
25	20.64025558548934	28.880594198522925	29.79556644525988	20.683583770727857
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13975.0
1	13975.0
2	13466.0
3	12957.0
4	12957.0
5	12957.0
6	15969.0
7	18981.0
8	18981.0
9	18981.0
10	17893.0
11	16805.0
12	16805.0
13	16805.0
14	17090.0
15	17375.0
16	17375.0
17	17375.0
18	23570.0
19	29765.0
20	29765.0
21	29765.0
22	38838.5
23	47912.0
24	47912.0
25	47912.0
26	62951.5
27	77991.0
28	77991.0
29	77991.0
30	92791.5
31	107592.0
32	107592.0
33	107592.0
34	116364.0
35	125136.0
36	125136.0
37	125136.0
38	133254.0
39	141372.0
40	141372.0
41	141372.0
42	147770.5
43	154169.0
44	154169.0
45	154169.0
46	157248.5
47	160328.0
48	160328.0
49	160328.0
50	152570.5
51	144813.0
52	144813.0
53	144813.0
54	129804.0
55	114795.0
56	114795.0
57	114795.0
58	99257.0
59	83719.0
60	83719.0
61	83719.0
62	69107.5
63	54496.0
64	54496.0
65	54496.0
66	42415.0
67	30334.0
68	30334.0
69	30334.0
70	22894.0
71	15454.0
72	15454.0
73	15454.0
74	11351.5
75	7249.0
76	7249.0
77	7249.0
78	5624.0
79	3999.0
80	3999.0
81	3999.0
82	2846.0
83	1693.0
84	1693.0
85	1693.0
86	1238.0
87	783.0
88	783.0
89	783.0
90	552.0
91	321.0
92	321.0
93	321.0
94	190.5
95	60.0
96	60.0
97	60.0
98	39.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3543179469962926
2	0.35757388075468205
3	0.36943995045192357
4	0.3717552811245561
5	0.37797773230725595
6	0.3151020337285796
7	0.3253763135883863
8	0.3237121696674317
9	0.32240979616407595
10	0.43028973469204657
11	0.3224821502475957
12	0.3219033175794376
13	0.31466790922746096
14	0.32096271449368063
15	0.31322082755706565
16	0.3170555939836132
17	0.31068843463387386
18	0.31264199488890754
19	0.31387201430874356
20	0.5102409969813876
21	0.31351024389114474
22	0.31640440723193536
23	0.3110502050514727
24	0.31466790922746096
25	0.30649189778972746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1382092.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.47609596160208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.89120080375457	58.42663898810827
2	6.8999576045420135	9.17364487701042
3	2.1822749288677876	4.3520735275804014
4	0.9521345248914501	2.5317674418015375
5	0.5002418787335723	1.6627063567352531
6	0.3155408558485523	1.2585554527916647
7	0.20145237481605385	0.9374237179975237
8	0.13875033325987926	0.7378864374790401
9	0.11289539007625556	0.6754360305898497
>10	0.7017314952557597	9.017063859616172
>50	0.06783442703679213	3.0878820629407233
>100	0.03331982812538236	3.925281900294137
>500	0.001110647829969632	0.4858853102796346
>1k	0.0013327773959635584	2.4667853064508236
>5k	2.221295659939264E-4	1.2609687303245525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	8730	0.6316511491275545	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8349	0.6040842433065238	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4912	0.355403258249089	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4512	0.3264616248411828	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	4127	0.298605302686073	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4023	0.29108047800001735	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3448	0.2494768799761521	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2872	0.20780092786876705	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2429	0.17574806886951086	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	2206	0.15961310824460312	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	1626	0.11764773980313901	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	85	1.02591E-9	15.6394005	2
ATAACGC	230	0.0	15.275127	3
TATAACG	220	0.0	14.674631	2
TCTGCGC	65	3.340383E-6	14.628426	18
TAACGCA	235	0.0	14.153322	4
ACCGAGT	55	1.9638099E-4	13.814932	8
CGCATAT	90	3.648711E-8	13.718994	8
CGCGCCA	70	7.284232E-6	13.567249	10
AAGGCGT	85	2.7023998E-7	13.410072	6
GTTATCA	260	0.0	13.146931	1
ATCGTAG	65	5.504766E-5	13.140246	13
ATATCGT	60	4.127354E-4	12.653572	11
GAGCGTT	55	0.0030919996	12.078409	11
CTCAACG	95	1.0456388E-6	11.994126	3
GTATTAG	170	1.8189894E-12	11.729124	1
AATGCGT	65	8.0923113E-4	11.68022	12
ATGCGTA	65	8.0923113E-4	11.68022	13
GTATCAA	4295	0.0	11.539913	1
TAGACAG	140	6.7666406E-10	11.53887	5
GGTATCA	1705	0.0	11.52766	1
>>END_MODULE