FastQCFastQC Report
Thu 26 May 2016
SRR1364293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382092
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT210491.522981104007548No Hit
GTACTTTTTTTTTTTTTTTTTTTTT155651.126191309985153No Hit
TATCAACGCAGAGTACTTTTTTTTT143061.0350975188337679No Hit
GGTATCAACGCAGAGTACTTTTTTT135600.9811213725280228No Hit
GAGTACTTTTTTTTTTTTTTTTTTT97600.7061758551529131No Hit
ACGCAGAGTACTTTTTTTTTTTTTT88570.6408401177345647No Hit
GCAGAGTACTTTTTTTTTTTTTTTT29290.2119251106293937No Hit
GTGGTATCAACGCAGAGTACTTTTT26280.19014653148994423No Hit
GTATAACGCAGAGTACTTTTTTTTT22950.16605262167786225No Hit
ATCAACGCAGAGTACTTTTTTTTTT22480.16265197975243326No Hit
GTGGTATCAACGCAGAGTACATGGG17810.1288626227487027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3100.017.1613882
TATCACG1050.016.2858092
ATGCGTA851.022272E-915.64431613
TAACGCA3500.015.2011884
TCGCGGT508.725855E-515.19733717
ATAACGC3600.014.7789333
CGAGCAA456.766931E-414.776257510
GTATAAC4000.014.016641
CTCGGAA551.9628202E-413.8157615
CGAAATC903.6459824E-813.71981813
ATCACGC1205.0931703E-1113.4593843
GAATGCG1001.0082658E-813.29863211
GCCCGAC500.001501381513.29766917
TGCGTAG1001.0091753E-813.29766914
GGCGTAG655.456896E-513.15249310
GCACGCC957.401286E-812.99772115
GTATCAC1403.6379788E-1212.8966671
GGTATCA32950.012.8338351
TTCGCGG604.0975685E-412.66444716
AGCGTTC604.1025758E-412.66261412
Produced by FastQC (version 0.11.5)