##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364293_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1382092 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.454884334762085 31.0 30.0 34.0 26.0 34.0 2 29.417137932930657 31.0 30.0 34.0 25.0 34.0 3 29.474005348413854 31.0 30.0 34.0 25.0 34.0 4 32.785041806189454 35.0 33.0 37.0 26.0 37.0 5 32.43200669709397 35.0 32.0 37.0 25.0 37.0 6 32.28227426249482 35.0 32.0 37.0 23.0 37.0 7 31.992049009762013 35.0 32.0 37.0 19.0 37.0 8 31.85403359544806 35.0 32.0 37.0 17.0 37.0 9 32.52155211085803 37.0 32.0 39.0 11.0 39.0 10 32.67202038648657 37.0 32.0 39.0 17.0 39.0 11 32.610601899149984 37.0 32.0 39.0 15.0 39.0 12 32.589900672314144 37.0 32.0 39.0 15.0 39.0 13 32.61566306729219 37.0 32.0 39.0 15.0 39.0 14 33.49515299994501 38.0 32.0 41.0 16.0 41.0 15 33.5330795634444 38.0 32.0 41.0 16.0 41.0 16 33.506035054106384 38.0 32.0 41.0 13.0 41.0 17 33.52995097287301 38.0 32.0 41.0 13.0 41.0 18 33.493458467308976 38.0 32.0 41.0 11.0 41.0 19 33.4274643077306 38.0 32.0 41.0 10.0 41.0 20 33.35136083560284 38.0 32.0 41.0 10.0 41.0 21 33.29075199046084 38.0 32.0 41.0 8.0 41.0 22 33.21973862810869 38.0 32.0 41.0 2.0 41.0 23 32.96144902075984 38.0 32.0 41.0 2.0 41.0 24 32.876307076518785 38.0 32.0 41.0 2.0 41.0 25 32.71698989647578 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.45063173938662615 1101 2 0.7915388259952927 1101 3 -1.3796602584958926 1101 4 -0.061922432787440584 1101 5 0.5354352254265109 1101 6 1.029044716629123 1101 7 1.1565381737106257 1101 8 1.0880955985705327 1101 9 1.8880511458487774 1101 10 1.5757076594931547 1101 11 1.544681915990651 1101 12 1.630341462470696 1101 13 1.4980154854473042 1101 14 1.5885185311906937 1101 15 1.5705237153158365 1101 16 1.5387243078770538 1101 17 1.5253786866883274 1101 18 1.4304590153676386 1101 19 1.5449047137566367 1101 20 1.916397788709041 1101 21 1.7910865274467298 1101 22 1.8385217391113855 1101 23 2.0620673048824685 1101 24 2.090561957996421 1101 25 2.1799593396382733 1102 1 0.8060203105618697 1102 2 0.79684538271826 1102 3 0.8844274868925588 1102 4 0.8892969024723243 1102 5 0.9911659600906191 1102 6 1.2638974331815334 1102 7 1.3917338597907367 1102 8 1.5134906583584637 1102 9 2.0987255182467806 1102 10 1.6019397906195536 1102 11 1.646958169683316 1102 12 1.8526586629365838 1102 13 1.582751124857765 1102 14 1.6878813574852884 1102 15 1.881695413679978 1102 16 1.9727544617901742 1102 17 1.740907831037795 1102 18 1.8925687414353405 1102 19 1.7539077646136576 1102 20 2.0465404172823156 1102 21 2.2159971578900155 1102 22 2.051747627466945 1102 23 2.3877110276534665 1102 24 2.225124500565343 1102 25 2.0386039047198636 1103 1 -1.9676677808723184 1103 2 -1.8192293376946687 1103 3 -1.8039767719922217 1103 4 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2212 9 0.5972016266161191 2212 10 0.33707862185234916 2212 11 0.47686211442251647 2212 12 0.19132726128261623 2212 13 0.1395589665248167 2212 14 0.19139017867770747 2212 15 0.4240991402324994 2212 16 0.48531025117438276 2212 17 0.3380054549659306 2212 18 0.37569551206764373 2212 19 0.4728006057380867 2212 20 0.6271890426303699 2212 21 0.7263676654263733 2212 22 0.44538636649215846 2212 23 0.8382562666684308 2212 24 0.797337459867272 2212 25 0.9169012745648715 2213 1 -0.17965083051809572 2213 2 -0.22742855344082713 2213 3 -0.20454069304327405 2213 4 -0.2916574903028035 2213 5 -0.1737257211816825 2213 6 -0.009005525452799645 2213 7 -0.20217234903287817 2213 8 0.009799162811262363 2213 9 0.6529769080477976 2213 10 0.025365833127182214 2213 11 0.23125812408864022 2213 12 -0.042207962178970604 2213 13 -0.03190902748701774 2213 14 -0.004751965111680079 2213 15 -0.09504084412653668 2213 16 -0.28258618063463103 2213 17 -0.2736929227384053 2213 18 -0.20454164369326833 2213 19 -0.1353787414537848 2213 20 0.31860259336142605 2213 21 0.09303793793544202 2213 22 0.04466162921544026 2213 23 -0.040565200788407196 2213 24 0.009076847854551318 2213 25 0.11960765686887953 2214 1 -0.8037219057086347 2214 2 -0.5830006166121713 2214 3 -0.5359387973086776 2214 4 -0.3617511999107279 2214 5 -0.8258444943026646 2214 6 -0.482270430591786 2214 7 -0.5312570553871545 2214 8 -0.38304280483050945 2214 9 -9.760494358796162 2214 10 -3.271879334680989 2214 11 -1.38617936569727 2214 12 -0.8615358009523888 2214 13 -0.6076706269759384 2214 14 -0.5964956398060011 2214 15 -0.631637361679914 2214 16 -0.7304419651508169 2214 17 -0.6301420974401566 2214 18 -1.092009887167393 2214 19 -1.1599153933131205 2214 20 -0.823532930862676 2214 21 -1.3293866263873078 2214 22 -1.0175009344833228 2214 23 -1.640835781245702 2214 24 -1.8925174783128256 2214 25 -9.523797942679472 2215 1 -0.6447470513990936 2215 2 -0.40761827976938747 2215 3 -0.29231134632828315 2215 4 -0.4520289776885065 2215 5 -0.45658477892225946 2215 6 -0.509682372379995 2215 7 -0.7312558480076525 2215 8 -0.43763889614059437 2215 9 0.4156530541920205 2215 10 -0.0016991936590642354 2215 11 0.27166860107564617 2215 12 0.2255488604983995 2215 13 0.14116425180316128 2215 14 -0.03664422797690037 2215 15 -0.41474290274691583 2215 16 -0.09983650457578364 2215 17 -0.33775140553120764 2215 18 -0.13862761471818175 2215 19 -0.20847901416375691 2215 20 -0.05489904052493699 2215 21 -0.07104846353595917 2215 22 0.007841156376009906 2215 23 0.4312524218516245 2215 24 0.4842907737236217 2215 25 0.39062662024915085 2216 1 -0.9186939233250371 2216 2 -0.8652168983207318 2216 3 -0.8428127515752699 2216 4 -0.7082723934411241 2216 5 -1.5487465367281885 2216 6 -2.168837203674851 2216 7 -1.5968192512830726 2216 8 -2.359803002534221 2216 9 -0.8348998533439271 2216 10 -1.3898270047388763 2216 11 -0.9958626166540583 2216 12 -2.267991221457418 2216 13 -1.9922709404555619 2216 14 -2.140464041829194 2216 15 -1.8888334499157722 2216 16 -2.88572159666802 2216 17 -3.160464279489119 2216 18 -2.6284677597369637 2216 19 -3.950446818203016 2216 20 -2.7624524839138154 2216 21 -2.7570425488443746 2216 22 -2.864236323063434 2216 23 -2.481418651411289 2216 24 -2.592699940762582 2216 25 -2.5229596731639248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54077.0 3 3.0 4 637.0 5 7424.0 6 10541.0 7 8267.0 8 6434.0 9 4990.0 10 4412.0 11 3839.0 12 3529.0 13 3250.0 14 3119.0 15 3241.0 16 3284.0 17 3604.0 18 4057.0 19 5201.0 20 7253.0 21 9453.0 22 11507.0 23 13519.0 24 15641.0 25 17838.0 26 19572.0 27 21490.0 28 23715.0 29 27073.0 30 33365.0 31 41063.0 32 50454.0 33 62693.0 34 77428.0 35 96972.0 36 126387.0 37 188181.0 38 408579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.649469405124364 19.3539212202488 11.72505031659105 23.27155905803578 2 16.10964109710558 22.44606471582905 38.154858679679 23.28943550738637 3 18.70873541245956 29.172259054603657 29.46478767255607 22.654217860380715 4 11.31556821322775 17.19964921268218 37.87540682790727 33.6093757461828 5 11.406486599167895 40.57758064095845 34.238496304167185 13.777436455706466 6 28.582934310911128 38.51057840582553 18.89177296209112 14.014714321172224 7 26.05380455195802 34.235865503817905 22.741407996503057 16.968921947721014 8 26.875475871333077 33.85779299922703 21.18796302461648 18.078768104823418 9 27.486198742451563 14.543163356077873 20.962856214311618 37.007781687158946 10 14.92702713853588 31.226217680112278 32.642245145095224 21.204510036256615 11 33.141966161960184 23.805704532079396 23.692708382345383 19.359620923615037 12 22.37466877186178 28.35262211120582 30.86279231652023 18.409916800412162 13 28.198493685824456 22.376775353117 27.892209321087748 21.5325216399708 14 21.367309238284545 21.856166321393864 30.54654887593985 26.229975564381743 15 23.172203601757463 29.33222478856607 26.320991727882653 21.174579881793818 16 21.143601432758057 28.887441658525997 28.17880531133543 21.790151597380515 17 20.163740117197055 28.319051540364093 29.717586649550704 21.799621692888145 18 19.682313395302725 26.357350392404467 33.179724502755334 20.78061170953747 19 21.050624318836093 26.21220037833997 33.11639815722095 19.62077714560299 20 21.05762870826416 25.811993521709365 33.17120721122325 19.959170558803223 21 21.776288033878167 25.039565291958205 32.99126632814552 20.192880346018107 22 20.96732642888983 25.490335129878023 33.02156721990981 20.52077122132234 23 20.657970716656077 25.364811649659245 33.312083105996905 20.66513452768777 24 20.46111866464735 25.79684179373612 33.26521884793417 20.476820693682356 25 20.509148697756522 25.359215227813834 33.46190257855581 20.66973349587383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1629.0 1 1629.0 2 2640.0 3 3651.0 4 3651.0 5 3651.0 6 11292.0 7 18933.0 8 18933.0 9 18933.0 10 17443.0 11 15953.0 12 15953.0 13 15953.0 14 13690.0 15 11427.0 16 11427.0 17 11427.0 18 18730.0 19 26033.0 20 26033.0 21 26033.0 22 37540.5 23 49048.0 24 49048.0 25 49048.0 26 67138.0 27 85228.0 28 85228.0 29 85228.0 30 98961.5 31 112695.0 32 112695.0 33 112695.0 34 120864.5 35 129034.0 36 129034.0 37 129034.0 38 132884.5 39 136735.0 40 136735.0 41 136735.0 42 143246.0 43 149757.0 44 149757.0 45 149757.0 46 156349.5 47 162942.0 48 162942.0 49 162942.0 50 156153.5 51 149365.0 52 149365.0 53 149365.0 54 134496.0 55 119627.0 56 119627.0 57 119627.0 58 104002.5 59 88378.0 60 88378.0 61 88378.0 62 73509.5 63 58641.0 64 58641.0 65 58641.0 66 45200.5 67 31760.0 68 31760.0 69 31760.0 70 24255.0 71 16750.0 72 16750.0 73 16750.0 74 11968.5 75 7187.0 76 7187.0 77 7187.0 78 5753.0 79 4319.0 80 4319.0 81 4319.0 82 3045.5 83 1772.0 84 1772.0 85 1772.0 86 1285.0 87 798.0 88 798.0 89 798.0 90 592.0 91 386.0 92 386.0 93 386.0 94 214.0 95 42.0 96 42.0 97 42.0 98 22.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024672742480240102 2 0.0013747275868755482 3 3.6177041759882843E-4 4 0.00419653684414641 5 0.03878178876659441 6 0.012517256448919465 7 0.02264682814168666 8 0.030099298744222524 9 0.03726235301267933 10 0.039867100019390894 11 0.04551071853393262 12 0.00824836552125329 13 0.0031112255913499243 14 0.006728929767338209 15 0.007235408351976569 16 0.010274279859806727 17 0.01056369619388579 18 0.01642437695898681 19 0.019246186216257673 20 0.01779910454586236 21 0.014398462620433372 22 0.013023735033557823 23 0.01056369619388579 24 0.007597178769575397 25 0.10947172836540549 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1382092.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.88028596360442 #Duplication Level Percentage of deduplicated Percentage of total 1 74.49829354737908 33.43504718206919 2 13.3145521321462 11.95121814336081 3 4.889898304404883 6.583801027039067 4 2.288845749462874 4.10896207049897 5 1.2539394831948467 2.813858129341953 6 0.7517251798313705 2.024258462212432 7 0.522953858009428 1.6429223085263316 8 0.36971215290715687 1.3274229717354435 9 0.27493309901009866 1.1105168493989876 >10 1.5598671667731057 13.511263308470012 >50 0.17319473937871466 5.449157432548278 >100 0.09560303405863094 7.66237125040867 >500 0.004213009738269478 1.222627364451688 >1k 0.0012963106886983006 1.1134694014465736 >5k 3.2407767217457515E-4 1.353893269331256 >10k+ 6.481553443491503E-4 4.6892108291604115 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 21049 1.522981104007548 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 15565 1.126191309985153 No Hit TATCAACGCAGAGTACTTTTTTTTT 14306 1.0350975188337679 No Hit GGTATCAACGCAGAGTACTTTTTTT 13560 0.9811213725280228 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 9760 0.7061758551529131 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 8857 0.6408401177345647 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2929 0.2119251106293937 No Hit GTGGTATCAACGCAGAGTACTTTTT 2628 0.19014653148994423 No Hit GTATAACGCAGAGTACTTTTTTTTT 2295 0.16605262167786225 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2248 0.16265197975243326 No Hit GTGGTATCAACGCAGAGTACATGGG 1781 0.1288626227487027 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 7.23540835197657E-5 0.0 7 0.0 0.0 0.0 7.23540835197657E-5 0.0 8 0.0 0.0 0.0 7.23540835197657E-5 0.0 9 0.0 0.0 0.0 7.23540835197657E-5 0.0 10 0.0 0.0 0.0 7.23540835197657E-5 0.0 11 0.0 0.0 0.0 7.23540835197657E-5 0.0 12 7.23540835197657E-5 0.0 0.0 7.23540835197657E-5 0.0 13 7.23540835197657E-5 0.0 0.0 7.23540835197657E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 310 0.0 17.161388 2 TATCACG 105 0.0 16.285809 2 ATGCGTA 85 1.022272E-9 15.644316 13 TAACGCA 350 0.0 15.201188 4 TCGCGGT 50 8.725855E-5 15.197337 17 ATAACGC 360 0.0 14.778933 3 CGAGCAA 45 6.766931E-4 14.7762575 10 GTATAAC 400 0.0 14.01664 1 CTCGGAA 55 1.9628202E-4 13.81576 15 CGAAATC 90 3.6459824E-8 13.719818 13 ATCACGC 120 5.0931703E-11 13.459384 3 GAATGCG 100 1.0082658E-8 13.298632 11 GCCCGAC 50 0.0015013815 13.297669 17 TGCGTAG 100 1.0091753E-8 13.297669 14 GGCGTAG 65 5.456896E-5 13.152493 10 GCACGCC 95 7.401286E-8 12.997721 15 GTATCAC 140 3.6379788E-12 12.896667 1 GGTATCA 3295 0.0 12.833835 1 TTCGCGG 60 4.0975685E-4 12.664447 16 AGCGTTC 60 4.1025758E-4 12.662614 12 >>END_MODULE