FastQCFastQC Report
Thu 26 May 2016
SRR1364292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663766
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63920.3841886419123843No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN46620.28020767343484604No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43290.2601928396180713No Hit
TATCAACGCAGAGTACTTTTTTTTT41660.25039578883088126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34450.20706036786423088No Hit
GGTATCAACGCAGAGTACTTTTTTT25810.15512998823151813No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20310.12207245490050884No Hit
GAACAAAAAAAAAAAAAAAAAAAAA20060.12056983974909934No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA19150.11510032059796871No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17720.1065053619319063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2100.016.293714
ATAACGC2100.015.8205673
CAACCGT508.700457E-515.2028736
ACAGTCG508.702697E-515.20241458
TATAACG2100.014.9165342
ACCGCCT456.754522E-414.7801268
CGCATAT902.2828317E-914.7801268
TTGACCG801.2780401E-714.25958118
CATTCCG954.8894435E-914.0026469
TCGGGAC707.323999E-613.5604883
GTGCGGC500.001512881413.28447111
GCAACGC802.8903867E-511.8654273
GTATAAC3950.011.5346491
TTGCGCG1251.8135324E-811.40766318
ACAACCG752.0645383E-411.4055985
GACTGCG600.005868993711.0850947
GCCGTGA600.005912644311.0740631
GTATCAA38150.011.0719911
CGGGACT700.001485396510.8624744
ACGCATA3250.010.5247497
Produced by FastQC (version 0.11.5)