##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364292_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1663766
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.026882987150838	30.0	25.0	31.0	2.0	34.0
2	24.944273413448766	30.0	23.0	31.0	2.0	34.0
3	24.40825452617736	30.0	18.0	31.0	2.0	34.0
4	28.016305778577035	35.0	26.0	37.0	2.0	37.0
5	28.56597742711415	35.0	28.0	37.0	2.0	37.0
6	28.737377131159068	35.0	28.0	37.0	2.0	37.0
7	28.356833232557943	35.0	27.0	37.0	2.0	37.0
8	27.856033841297393	35.0	26.0	37.0	2.0	37.0
9	28.746367578132983	35.0	25.0	39.0	2.0	39.0
10	28.084382659580733	35.0	22.0	39.0	2.0	39.0
11	28.692524068889494	35.0	26.0	39.0	2.0	39.0
12	28.22024611634088	35.0	23.0	39.0	2.0	39.0
13	27.895254500933426	35.0	19.0	39.0	2.0	39.0
14	28.091057877129355	34.0	17.0	40.0	2.0	41.0
15	28.757052974997684	35.0	24.0	40.0	2.0	41.0
16	29.082526028299654	36.0	25.0	40.0	2.0	41.0
17	29.240734574453377	36.0	26.0	40.0	2.0	41.0
18	29.06429509919063	36.0	25.0	40.0	2.0	41.0
19	29.0553719693755	36.0	25.0	40.0	2.0	41.0
20	28.8738428360719	36.0	24.0	40.0	2.0	41.0
21	28.816152632040804	36.0	23.0	40.0	2.0	41.0
22	28.8730548646865	36.0	24.0	40.0	2.0	41.0
23	28.855863745262255	36.0	25.0	40.0	2.0	41.0
24	28.686734793234145	36.0	24.0	40.0	2.0	41.0
25	28.501436500084747	36.0	22.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.49253966529929016
1101	2	0.6331412672820385
1101	3	1.3525138298005501
1101	4	0.8411076368695909
1101	5	0.3084398270570148
1101	6	0.21375338749889394
1101	7	0.4404369380085207
1101	8	0.759555115665858
1101	9	0.7683353714407097
1101	10	1.1426944856508499
1101	11	0.42596464655987987
1101	12	0.7336496700624373
1101	13	0.5253117929199789
1101	14	1.085209956451525
1101	15	0.43349597796727934
1101	16	-0.10452912985507012
1101	17	-0.6239580041685713
1101	18	-6.152215722388231
1101	19	-7.524951006401654
1101	20	-8.446919783328937
1101	21	-10.160131334309185
1101	22	-10.456953490145455
1101	23	-11.914907901973645
1101	24	-11.58387998995877
1101	25	-11.969971640807909
1102	1	1.7227535871113666
1102	2	1.946291267969265
1102	3	2.5991590244915024
1102	4	2.065243622740237
1102	5	1.4396772565591434
1102	6	1.5657001252399354
1102	7	1.7988541891985648
1102	8	1.9993987833419844
1102	9	2.363056220484779
1102	10	-1.1722646358918354
1102	11	1.0437175684131894
1102	12	1.8494139334255344
1102	13	2.4426314191609926
1102	14	2.9075292619873565
1102	15	2.5185552969727247
1102	16	2.268946661484975
1102	17	2.049363676876375
1102	18	2.2713665316194387
1102	19	1.791987896969875
1102	20	2.3336723415261744
1102	21	2.318321572425166
1102	22	2.1959269578338727
1102	23	2.2621582984487674
1102	24	2.4091896282646132
1102	25	2.475159997216732
1103	1	-1.1235432260419884
1103	2	-0.6685912551201483
1103	3	-3.0760498797339437
1103	4	-1.625893171467954
1103	5	-4.8511203854449825
1103	6	-7.945013616767575
1103	7	-8.065184682211157
1103	8	-2.900134776656664
1103	9	-7.461290848089934
1103	10	-5.478484822636986
1103	11	-8.098335190475812
1103	12	-2.7559262336978456
1103	13	-0.4741219385304021
1103	14	-0.7836794223389063
1103	15	-1.055565529171595
1103	16	-0.8447264160886938
1103	17	-1.0550461475425834
1103	18	-0.44155085394840654
1103	19	-5.427312908199447
1103	20	-2.355514961833208
1103	21	-6.388123588101237
1103	22	-3.194349692255777
1103	23	-2.4302598313932435
1103	24	-7.5292026549655375
1103	25	-4.636897667115619
1104	1	1.4887725268854481
1104	2	1.6125563634765747
1104	3	2.5139763172802283
1104	4	1.974359329873458
1104	5	1.6771376709259407
1104	6	1.6422098153569102
1104	7	1.6053894427138822
1104	8	1.8504317732281308
1104	9	1.9475088236583211
1104	10	2.204670181623971
1104	11	1.7477147416644527
1104	12	1.9727421373528742
1104	13	2.0888739898854674
1104	14	2.6248929982020783
1104	15	1.7927316649072473
1104	16	0.9296636353584553
1104	17	0.776775334009038
1104	18	0.6054522707556913
1104	19	1.2129358056218429
1104	20	-9.090792555495426
1104	21	-3.6024593517599826
1104	22	-1.0151936779894513
1104	23	0.027714476595267712
1104	24	0.6166736825025048
1104	25	1.050549859949367
1105	1	1.2081804950557213
1105	2	1.2018596077691797
1105	3	1.9225441207632983
1105	4	1.4962251725389706
1105	5	1.3674546683516446
1105	6	1.3708411709179273
1105	7	1.6602789118322043
1105	8	1.9018486256906684
1105	9	1.8321472493526905
1105	10	2.343478347034573
1105	11	2.040584418930667
1105	12	1.988117558042763
1105	13	1.9026580654132523
1105	14	2.639950799791869
1105	15	2.187316598345703
1105	16	1.6775230973840394
1105	17	0.8153082672101242
1105	18	1.3555929952324277
1105	19	1.3363784406170254
1105	20	1.3331697427782991
1105	21	1.2575559640364808
1105	22	1.1309077171044066
1105	23	1.1628919222482104
1105	24	0.9368711771546927
1105	25	1.069878564634248
1106	1	1.0646205393298516
1106	2	1.3685697782081618
1106	3	1.9847801286303444
1106	4	1.5372803668014008
1106	5	0.964478687713072
1106	6	0.5167148430019388
1106	7	1.2897254617288816
1106	8	1.0731104149685642
1106	9	1.3173633966234206
1106	10	-2.0431857719729756
1106	11	0.061595274539399725
1106	12	0.6874925114839492
1106	13	1.6962453258663324
1106	14	0.5906389317086003
1106	15	1.3653067592588215
1106	16	0.9353517234136852
1106	17	-5.906972008922494
1106	18	-2.0564178879449884
1106	19	-0.41258040168600374
1106	20	0.4353493990848101
1106	21	0.7326781607560413
1106	22	1.0927485543007762
1106	23	0.6193864879916013
1106	24	0.9070027090994657
1106	25	0.7899781286384666
1107	1	0.7449527298593033
1107	2	1.21380146035046
1107	3	1.5976574683333027
1107	4	1.178114006592594
1107	5	0.7402603608672464
1107	6	0.8137378643513813
1107	7	0.7054801470093857
1107	8	0.9152877500893375
1107	9	0.9508745048886169
1107	10	-1.8544250111898712
1107	11	-0.12238298124736957
1107	12	0.1942394799009719
1107	13	1.1123925807284358
1107	14	1.489833877679981
1107	15	1.3717952456468083
1107	16	-5.303154197587155
1107	17	-1.9119120998376715
1107	18	-0.1814277278858647
1107	19	0.5385741317950554
1107	20	0.9260715097550651
1107	21	1.2024071247248216
1107	22	0.8667346585418798
1107	23	0.667356640366755
1107	24	1.0036545737535754
1107	25	1.0956337154337916
1108	1	-0.9267334229831299
1108	2	-3.477750758400312
1108	3	-0.5093225787491278
1108	4	-1.0021061314514945
1108	5	-1.3402795384908188
1108	6	-0.9468917483549895
1108	7	-0.6768645592789078
1108	8	-0.32767019957556087
1108	9	-0.9266712791846139
1108	10	-3.918289391881597
1108	11	-2.0340146560182255
1108	12	-6.00295088362769
1108	13	-2.4055394900859923
1108	14	-5.987415109246928
1108	15	-3.1719308848673897
1108	16	-7.830307706570856
1108	17	-3.842810711271003
1108	18	-2.3145077291577287
1108	19	-1.01146655599943
1108	20	-1.2072739043006528
1108	21	-0.6622760757500075
1108	22	-0.6711740300392783
1108	23	-0.6551715613975979
1108	24	-0.45507337034104367
1108	25	-0.7129269729730723
1109	1	-8.54934692646906
1109	2	-6.5983315123806605
1109	3	-5.404930056078111
1109	4	-5.430431615217941
1109	5	-2.766141250999528
1109	6	-1.8642330519655452
1109	7	-1.3893061967512779
1109	8	-0.9580873427910319
1109	9	-5.472418436659812
1109	10	-1.8076853547245406
1109	11	-1.4539597674895468
1109	12	-5.935826138439747
1109	13	-7.771916999960208
1109	14	-7.874702884684378
1109	15	-4.111199228898549
1109	16	-2.5178532366534085
1109	17	-2.023483681354964
1109	18	-1.4831376968743797
1109	19	-1.2434032527622065
1109	20	-0.995592101798966
1109	21	-0.8787473483139046
1109	22	-1.1878874167965137
1109	23	-1.3359140041859163
1109	24	-1.095788914462176
1109	25	-1.026653920947691
1110	1	-2.357352990777631
1110	2	-2.2137152116088927
1110	3	-1.4434564907466765
1110	4	-2.4884933063865837
1110	5	-5.378243245719879
1110	6	-3.8227204217258333
1110	7	-8.393700998932637
1110	8	-9.32728925848481
1110	9	-10.873968982839912
1110	10	-8.84954477710195
1110	11	-11.426119995265424
1110	12	-6.635629412718
1110	13	-4.94444405675285
1110	14	-4.4801293759606295
1110	15	-9.750752728766841
1110	16	-11.290921289180858
1110	17	-11.782645439219788
1110	18	-8.864995073528473
1110	19	-11.945699787362955
1110	20	-12.829401883158784
1110	21	-13.76047791814593
1110	22	-14.863369002484296
1110	23	-15.604370211695123
1110	24	-15.788693337147846
1110	25	-16.468801706242022
1111	1	-0.7277171855753295
1111	2	-0.009897415497448492
1111	3	-2.5821737661795012
1111	4	-3.2516858397767088
1111	5	-1.1397800042852957
1111	6	-0.8499634312370468
1111	7	-0.24172827711177192
1111	8	0.2123970732810676
1111	9	-4.602347088556169
1111	10	-4.14264592550937
1111	11	-1.3739747881429452
1111	12	-5.722157343847183
1111	13	-1.4403360761900252
1111	14	-0.08311445058749456
1111	15	0.0367542070759157
1111	16	-0.11004249688622991
1111	17	-0.14139581053258254
1111	18	0.18340379768063286
1111	19	0.35592081783486407
1111	20	0.4460151081146648
1111	21	0.5388268161622491
1111	22	0.03441825350014582
1111	23	0.10419114038787214
1111	24	0.17195423236471186
1111	25	0.31544146020138797
1112	1	-0.379105171543646
1112	2	0.2317492787490778
1112	3	-2.494947463257361
1112	4	-0.55652851160335
1112	5	-0.47681529208356466
1112	6	-0.3607492539277679
1112	7	0.19655468983663127
1112	8	-3.4516747177686433
1112	9	-1.0401962718618272
1112	10	-3.257499665261232
1112	11	-0.8413344301440233
1112	12	0.26439164099172174
1112	13	0.6852806517670729
1112	14	1.289741058371714
1112	15	0.8093258812331037
1112	16	0.5853715223998179
1112	17	0.3669121483664881
1112	18	0.37963978686267197
1112	19	0.5364668669749655
1112	20	0.5381570530029549
1112	21	0.5341502562698288
1112	22	0.35584620626039154
1112	23	0.2990685134743032
1112	24	0.4808152009849742
1112	25	0.5276786256099655
1113	1	-1.167358772267736
1113	2	-0.8554206751170383
1113	3	-3.3290518320828113
1113	4	-1.5191964420842403
1113	5	-1.8403219957008616
1113	6	-1.514408388619195
1113	7	-1.2625619693101058
1113	8	-8.01423488204895
1113	9	-9.165769156284618
1113	10	-3.6639406874584246
1113	11	-9.078293379110722
1113	12	-7.59015799305789
1113	13	-3.3602982719155676
1113	14	-1.7069676615966678
1113	15	-7.056412122927533
1113	16	-3.383401928796239
1113	17	-2.568695312179152
1113	18	-1.8329363305461364
1113	19	-1.5822395655363053
1113	20	-6.631055313256006
1113	21	-2.9950276465680403
1113	22	-2.205554101454279
1113	23	-2.1851882164724614
1113	24	-6.750232734960008
1113	25	-4.278131966256932
1114	1	-4.621885590934742
1114	2	-4.456547104774643
1114	3	-1.3157856345015873
1114	4	-1.037570289890116
1114	5	-1.1727837013365345
1114	6	-1.074036211199516
1114	7	-0.9568392550206148
1114	8	-0.7449051265130926
1114	9	-1.26505303625704
1114	10	-3.978924195797944
1114	11	-2.0200712914162473
1114	12	-5.257938917824049
1114	13	-2.0403984950874943
1114	14	-6.056013115159374
1114	15	-2.6564282982876577
1114	16	-1.8573195609797608
1114	17	-1.765991864985697
1114	18	-1.2888953091922595
1114	19	-1.1826597245192758
1114	20	-0.9049910426246122
1114	21	-0.8557253651215788
1114	22	-0.9047672742831985
1114	23	-1.1566679336118568
1114	24	-1.0478449475170528
1114	25	-0.9300350133904907
1115	1	0.006420722081472263
1115	2	0.09854360888454039
1115	3	0.776369139845837
1115	4	-0.412301136956291
1115	5	-0.5846499936449128
1115	6	-1.2057661400581772
1115	7	-3.1939150973188717
1115	8	-1.007540745320835
1115	9	-0.8680909544054884
1115	10	-0.04990365820908238
1115	11	-0.9939551260049697
1115	12	-0.2245461078911113
1115	13	-0.026356268912799408
1115	14	0.6599494818612399
1115	15	-0.18375073631654715
1115	16	-0.6508095739332127
1115	17	-0.921981329130908
1115	18	-0.4995882415844548
1115	19	-0.28327577953718475
1115	20	-0.3016211547141552
1115	21	-0.1918419064072765
1115	22	-0.5041164113987122
1115	23	-0.7238270589426001
1115	24	-0.8206367563605887
1115	25	-0.6053982770670281
1116	1	-0.3608987874036522
1116	2	-0.320598435964488
1116	3	0.4816511263147092
1116	4	-0.09475791818586643
1116	5	-1.0274528169586645
1116	6	-2.0464809700533166
1116	7	-5.366072173888291
1116	8	-2.0998470933779743
1116	9	-2.041630055893343
1116	10	-1.3856395394908994
1116	11	-3.186960744280171
1116	12	-1.5642741057124994
1116	13	-7.092832027391946
1116	14	-2.7678484137898813
1116	15	-2.328673604250188
1116	16	-2.0472593774565375
1116	17	-1.705763810565447
1116	18	-1.4584410089433923
1116	19	-1.1428716852083838
1116	20	-1.215884623608698
1116	21	-1.086904586395928
1116	22	-1.240758286793728
1116	23	-1.3059716648965463
1116	24	-1.1754252667588645
1116	25	-1.1895417405310447
1201	1	2.300489521195125
1201	2	2.4756543750248987
1201	3	3.100861076320694
1201	4	2.745936272160968
1201	5	2.304536768744235
1201	6	2.2155150984983045
1201	7	2.601956207215185
1201	8	2.649614424170206
1201	9	3.096392961729496
1201	10	2.9719384211760236
1201	11	2.6520921488973954
1201	12	3.1532924411944308
1201	13	3.1977094451007204
1201	14	3.8539324780656905
1201	15	3.3293632404143416
1201	16	2.841855450926314
1201	17	2.6817084874460058
1201	18	2.603153263382925
1201	19	2.7260852212073203
1201	20	2.844783798505386
1201	21	2.628189674831571
1201	22	1.749702799892603
1201	23	1.19115089509447
1201	24	1.7288025485514709
1201	25	1.8915891096138395
1202	1	2.1613426227407757
1202	2	2.431425151356624
1202	3	3.0944617920019013
1202	4	2.646023147086929
1202	5	2.0791794324010553
1202	6	2.0613470480733476
1202	7	2.2842101712032807
1202	8	2.5516449053942303
1202	9	2.7380941193705546
1202	10	-1.0995520008491866
1202	11	1.1270056645961013
1202	12	2.261233258290325
1202	13	2.8059471369435975
1202	14	3.238925757900965
1202	15	2.9638067337863667
1202	16	2.7040618183640674
1202	17	2.1933983922557445
1202	18	2.4603888964207385
1202	19	2.602298678979942
1202	20	2.599562842727792
1202	21	2.6506672124927597
1202	22	2.439095962341611
1202	23	2.2332980182645343
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2211	14	-4.940612673757361
2211	15	-1.2781692177504915
2211	16	-0.14093905748221047
2211	17	0.2893517178519929
2211	18	0.8994860920720953
2211	19	1.1844707883300103
2211	20	1.3178795667346073
2211	21	1.3029549176749633
2211	22	1.2092678134360995
2211	23	0.8860882104797163
2211	24	1.212301687727777
2211	25	1.2704662397423405
2212	1	1.1612349694918898
2212	2	1.2121220267210724
2212	3	1.7996623410334713
2212	4	1.4049740661765426
2212	5	1.080089999464544
2212	6	0.8618969767530693
2212	7	1.13331620068233
2212	8	1.3939508379853542
2212	9	1.4248859628427297
2212	10	2.169841283382304
2212	11	1.2867765425980586
2212	12	1.5902260724359607
2212	13	1.7738530121196554
2212	14	-3.656112324435828
2212	15	-0.5539919366960113
2212	16	0.22434548467488113
2212	17	0.5647931298060946
2212	18	0.7611182471819369
2212	19	1.3803804775068862
2212	20	1.7573152223131459
2212	21	1.6307154770326768
2212	22	1.5590127104958071
2212	23	1.4430379459574354
2212	24	1.4565429195558792
2212	25	1.5840098913335297
2213	1	1.0862032919797038
2213	2	1.2588264916395708
2213	3	1.8851271951021538
2213	4	1.4416254846847991
2213	5	0.9224498130504379
2213	6	0.76388997548597
2213	7	0.9449606393997669
2213	8	-4.82371554948968
2213	9	-1.389941260489099
2213	10	0.7337632815353423
2213	11	0.5969305275217103
2213	12	1.1800355778372449
2213	13	-7.009872263313628
2213	14	-1.704504824919045
2213	15	-0.2326101528762372
2213	16	0.2829692846877663
2213	17	0.4637429397410209
2213	18	0.9178134773002711
2213	19	1.151417567041495
2213	20	1.3603133109242762
2213	21	1.4527114824446983
2213	22	0.9411831032969573
2213	23	0.9744835143271722
2213	24	1.0149648425419429
2213	25	1.1349322975358795
2214	1	-1.037613006767213
2214	2	-0.719288401000707
2214	3	-6.578732443370928
2214	4	-2.5428709834500047
2214	5	-1.7104185698795007
2214	6	-1.252238801801333
2214	7	-0.8508461440830821
2214	8	-5.953599662830587
2214	9	-1.9593297808364518
2214	10	-0.23373521194440983
2214	11	-0.5513607655184494
2214	12	-0.27561607041920766
2214	13	-5.663224136488971
2214	14	-1.5818728755608902
2214	15	-0.798959327910179
2214	16	-0.6363201815582755
2214	17	-0.6110793604261033
2214	18	-0.5314012035735303
2214	19	-0.05593867804611108
2214	20	0.05020650448794228
2214	21	-0.018803618484252382
2214	22	-0.02450743942340239
2214	23	-0.13353041458277914
2214	24	0.0631017844417947
2214	25	0.12316670639503968
2215	1	0.055643625836040655
2215	2	0.28555351300156673
2215	3	-2.3520764878046023
2215	4	-0.6295768386871714
2215	5	-0.23361140115196832
2215	6	-0.6753499013180146
2215	7	-0.18865392573154338
2215	8	0.11823001776471997
2215	9	0.40199369806941476
2215	10	0.8853437069372916
2215	11	0.2149457148496552
2215	12	0.02668600036065527
2215	13	0.5165660934061336
2215	14	0.9358393022555695
2215	15	0.5539678872584162
2215	16	0.28539698676314984
2215	17	0.2322991705592159
2215	18	0.2976776319358656
2215	19	0.4241003778458783
2215	20	0.6988790963259
2215	21	0.8023155821313033
2215	22	0.46189776451467424
2215	23	0.41729845407383337
2215	24	0.395973925477616
2215	25	0.508887746418857
2216	1	0.13732963635515816
2216	2	-0.09111429004222771
2216	3	1.077379764220101
2216	4	-0.3245470978320384
2216	5	-0.42884760646648346
2216	6	-0.7457871351386345
2216	7	-1.3738564298318323
2216	8	-5.97879097807478
2216	9	-2.152914857493492
2216	10	-0.9021074670081255
2216	11	-1.2723919909914905
2216	12	-0.13115847879700127
2216	13	0.16390179786666792
2216	14	0.9956327529919236
2216	15	0.5580847950236034
2216	16	-0.25023963036086627
2216	17	-0.2654128104174909
2216	18	0.09140330371283767
2216	19	0.13557337701649175
2216	20	0.23378470070044344
2216	21	0.021666727752094772
2216	22	-0.21961795904378079
2216	23	-0.43529528431858466
2216	24	-0.35358365068085007
2216	25	-0.35687643327798213
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	203493.0
3	3.0
4	1363.0
5	8791.0
6	13151.0
7	11395.0
8	9952.0
9	8071.0
10	7313.0
11	6652.0
12	5986.0
13	5986.0
14	6047.0
15	6031.0
16	6176.0
17	6718.0
18	7764.0
19	10050.0
20	13820.0
21	17319.0
22	20885.0
23	24155.0
24	27858.0
25	30669.0
26	33081.0
27	36508.0
28	39036.0
29	44914.0
30	50676.0
31	56524.0
32	68838.0
33	85900.0
34	104766.0
35	127667.0
36	156330.0
37	178687.0
38	221191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.68379839009766	18.84081482986986	12.584244861478078	22.891141918554407
2	13.882028540256524	22.408160286693217	41.573592090606056	22.136219082444203
3	16.085336312266868	29.80974313936487	30.59925301472256	23.505667533645703
4	9.847399567755762	17.87570595569346	39.71562117164367	32.56127330490711
5	11.128222482411582	41.10867208721129	34.63243025105458	13.130675179322546
6	27.77310536796584	39.50061283929985	19.960534716607796	12.765747076126518
7	26.26231200378163	34.21802496647662	23.132820112098322	16.386842917643428
8	24.897576998883974	36.308057034915166	21.932962450024508	16.861403516176352
9	27.047971503128	16.664375533592835	20.672833432213814	35.61481953106535
10	12.490304386431394	32.30708218922578	34.738334630105925	20.464278794236897
11	32.379782125828726	25.70765694826634	23.9554870093068	17.957073916598134
12	21.251163654429604	28.98651125549269	31.675819140366873	18.086505949710833
13	26.81344042134252	24.518628466423664	27.300870924062124	21.367060188171685
14	20.11394008528926	23.613175279499284	30.639560928523064	25.633323706688387
15	22.34622591232339	31.624819440391818	24.85531116539622	21.173643481888575
16	20.820039405493134	31.009397993069072	27.16320240773833	21.007360193699462
17	20.14559068219634	30.420012539487328	27.84356989558465	21.59082688273168
18	19.45832052232732	29.75215978489567	30.509425886069465	20.28009380670754
19	21.116502220416255	29.089623306955215	29.839006256714605	19.95486821591393
20	21.335408172943033	28.768162649383317	30.171156145171917	19.725273032501725
21	22.489897129476507	28.105061413033322	29.234629559404517	20.170411898085653
22	21.245066794963158	29.02798527484267	29.266611923615354	20.460336006578814
23	21.121419601511768	28.899530300189486	29.013473650449534	20.965576447849212
24	20.35060768824047	28.931675693012988	29.921991387038837	20.795725231707706
25	20.789187331767938	28.707405074165283	29.744664048300123	20.758743545766656
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8922.0
1	8922.0
2	8100.0
3	7278.0
4	7278.0
5	7278.0
6	11309.5
7	15341.0
8	15341.0
9	15341.0
10	15463.0
11	15585.0
12	15585.0
13	15585.0
14	17856.5
15	20128.0
16	20128.0
17	20128.0
18	28273.0
19	36418.0
20	36418.0
21	36418.0
22	49412.0
23	62406.0
24	62406.0
25	62406.0
26	81896.5
27	101387.0
28	101387.0
29	101387.0
30	119274.5
31	137162.0
32	137162.0
33	137162.0
34	150287.5
35	163413.0
36	163413.0
37	163413.0
38	174650.0
39	185887.0
40	185887.0
41	185887.0
42	193859.5
43	201832.0
44	201832.0
45	201832.0
46	202004.5
47	202177.0
48	202177.0
49	202177.0
50	189801.5
51	177426.0
52	177426.0
53	177426.0
54	155609.5
55	133793.0
56	133793.0
57	133793.0
58	112184.5
59	90576.0
60	90576.0
61	90576.0
62	72544.0
63	54512.0
64	54512.0
65	54512.0
66	40897.5
67	27283.0
68	27283.0
69	27283.0
70	19922.5
71	12562.0
72	12562.0
73	12562.0
74	8995.0
75	5428.0
76	5428.0
77	5428.0
78	3973.0
79	2518.0
80	2518.0
81	2518.0
82	1775.0
83	1032.0
84	1032.0
85	1032.0
86	735.5
87	439.0
88	439.0
89	439.0
90	321.5
91	204.0
92	204.0
93	204.0
94	120.5
95	37.0
96	37.0
97	37.0
98	28.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3397713380367191
2	0.3418148946426361
3	0.35473738494475787
4	0.35503790797503976
5	0.3605675317322268
6	0.30611275864514603
7	0.3138662528264191
8	0.3127242653113479
9	0.31242374228106595
10	0.4260815523336815
11	0.31218332385684044
12	0.31242374228106595
13	0.3046101434937365
14	0.3110413363417692
15	0.30256658688781957
16	0.30737495537233
17	0.3008836579182409
18	0.3026867960999323
19	0.3046101434937365
20	0.5034962849342997
21	0.3052712941603567
22	0.3070143277359917
23	0.3033479467665525
24	0.30671380470570986
25	0.2987799967062676
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1663766.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.7082213219307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34965085344211	64.23745967967372
2	7.253738033856153	10.548127807538389
3	2.027753580864334	4.423030684314644
4	0.8043194160265646	2.3392253645594203
5	0.4018153584663035	1.4607640006959455
6	0.2579127361205344	1.1251425779757913
7	0.15474103399219558	0.7875661742962269
8	0.10510177581117748	0.6113410541605626
9	0.08867129245160169	0.5802418760825429
>10	0.4923616017943536	6.70926219273249
>50	0.04121951075556585	2.091765042476891
>100	0.021200702797269193	2.698391890000328
>500	6.72934943076896E-4	0.3511188622998279
>1k	7.57051810961508E-4	1.6456288500089653
>5k	8.4116867884612E-5	0.3909339431842013
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6392	0.3841886419123843	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4662	0.28020767343484604	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4329	0.2601928396180713	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4166	0.25039578883088126	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3445	0.20706036786423088	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2581	0.15512998823151813	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2031	0.12207245490050884	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	2006	0.12056983974909934	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1915	0.11510032059796871	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1772	0.1065053619319063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	210	0.0	16.29371	4
ATAACGC	210	0.0	15.820567	3
CAACCGT	50	8.700457E-5	15.202873	6
ACAGTCG	50	8.702697E-5	15.2024145	8
TATAACG	210	0.0	14.916534	2
ACCGCCT	45	6.754522E-4	14.780126	8
CGCATAT	90	2.2828317E-9	14.780126	8
TTGACCG	80	1.2780401E-7	14.259581	18
CATTCCG	95	4.8894435E-9	14.002646	9
TCGGGAC	70	7.323999E-6	13.560488	3
GTGCGGC	50	0.0015128814	13.284471	11
GCAACGC	80	2.8903867E-5	11.865427	3
GTATAAC	395	0.0	11.534649	1
TTGCGCG	125	1.8135324E-8	11.407663	18
ACAACCG	75	2.0645383E-4	11.405598	5
GACTGCG	60	0.0058689937	11.085094	7
GCCGTGA	60	0.0059126443	11.074063	1
GTATCAA	3815	0.0	11.071991	1
CGGGACT	70	0.0014853965	10.862474	4
ACGCATA	325	0.0	10.524749	7
>>END_MODULE