FastQCFastQC Report
Thu 26 May 2016
SRR1364292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663766
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT147940.8891875419980935No Hit
TATCAACGCAGAGTACTTTTTTTTT120340.7232988292824832No Hit
GTACTTTTTTTTTTTTTTTTTTTTT113480.6820670695278062No Hit
GGTATCAACGCAGAGTACTTTTTTT98820.5939537170491523No Hit
GAGTACTTTTTTTTTTTTTTTTTTT62930.3782382859128026No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57450.3453009617939061No Hit
GCAGAGTACTTTTTTTTTTTTTTTT19600.11780502787050583No Hit
GTATAACGCAGAGTACTTTTTTTTT19520.11732419102205478No Hit
ATCAACGCAGAGTACTTTTTTTTTT18220.10951059223472531No Hit
GTGGTATCAACGCAGAGTACTTTTT18180.10927017381049979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTG250.006027567719.0025569
CGACGGG555.9224294E-717.26933915
TATAACG3000.016.1482872
TAACGCA3300.014.68335154
ATAACGC3450.014.3194753
GCACCGT903.6328856E-813.7240686
AATGCGT802.0047282E-613.05954512
TCACGCA1650.012.667994
GTATAAC4900.012.6010841
TGCACCG701.08839726E-412.2173985
ACGCATC550.003067583312.09144410
TATCACG1750.011.9415912
CCGACGG802.8724056E-511.8726714
GGTATCA27700.011.6940581
CGAGACG658.0391864E-411.68966318
GAATGCG907.4651434E-611.61057911
ACGGGCG907.4801465E-611.60848417
GGGCGCT1001.9126437E-611.40839519
ATCACGC1751.8189894E-1211.4005053
CAGTCCG700.001489824810.85860359
Produced by FastQC (version 0.11.5)