##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364292_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1663766 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.569256133374527 31.0 30.0 34.0 26.0 34.0 2 29.577890761080585 31.0 30.0 34.0 25.0 34.0 3 29.63051775309749 31.0 30.0 34.0 26.0 34.0 4 32.97508904497387 35.0 33.0 37.0 27.0 37.0 5 32.594635303281834 35.0 33.0 37.0 25.0 37.0 6 32.47720592919918 35.0 33.0 37.0 25.0 37.0 7 32.1955311023305 35.0 32.0 37.0 22.0 37.0 8 32.06678403092742 35.0 32.0 37.0 19.0 37.0 9 32.75218510295318 37.0 32.0 39.0 16.0 39.0 10 32.90290100891592 37.0 32.0 39.0 17.0 39.0 11 32.86079713132736 37.0 32.0 39.0 17.0 39.0 12 32.75950524292479 37.0 32.0 39.0 17.0 39.0 13 32.86390994887502 37.0 32.0 39.0 17.0 39.0 14 33.77821821097438 38.0 33.0 41.0 17.0 41.0 15 33.82504090118442 38.0 33.0 41.0 17.0 41.0 16 33.773171828249886 38.0 33.0 41.0 17.0 41.0 17 33.75882305564605 38.0 33.0 41.0 17.0 41.0 18 33.71636456088176 38.0 33.0 41.0 16.0 41.0 19 33.63552506782805 39.0 32.0 41.0 11.0 41.0 20 33.47189328306985 39.0 32.0 41.0 10.0 41.0 21 33.394049403581995 38.0 32.0 41.0 10.0 41.0 22 33.36668497853665 38.0 32.0 41.0 2.0 41.0 23 33.07207984776706 38.0 32.0 41.0 2.0 41.0 24 32.992037341789654 38.0 32.0 41.0 2.0 41.0 25 32.858638173877814 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.27663422657689196 1101 2 0.5555650697413697 1101 3 -1.2998296502100892 1101 4 -0.3632844465686702 1101 5 0.4314526288341085 1101 6 0.7695167469200257 1101 7 0.8730541970469261 1101 8 0.8780691263159603 1101 9 1.5717533867206654 1101 10 1.124198131013138 1101 11 1.3198695923368078 1101 12 1.4704448622554622 1101 13 1.0014257874663102 1101 14 1.1813604146823877 1101 15 1.3387744026046562 1101 16 1.2137398233671348 1101 17 1.3378840558832081 1101 18 1.3246952298775767 1101 19 1.2319458341173402 1101 20 1.6479194426641044 1101 21 1.5054040189959537 1101 22 1.6321949754767004 1101 23 1.9526020912216637 1101 24 1.9035009357558508 1101 25 1.9660854730819182 1102 1 0.6596865038127788 1102 2 0.620595777262821 1102 3 0.6618558158890799 1102 4 0.8060123844059461 1102 5 0.7879044153910613 1102 6 0.9016386927758262 1102 7 0.9151413120289007 1102 8 1.1281352717603461 1102 9 1.6987088293167218 1102 10 1.2275184605148013 1102 11 1.374134341339186 1102 12 1.429051272235803 1102 13 1.238597293726933 1102 14 1.2477930620120574 1102 15 1.2674235121896231 1102 16 1.2630937742259363 1102 17 1.3716480122856254 1102 18 1.2989354449333135 1102 19 1.2957426857087242 1102 20 1.5495875105069956 1102 21 1.6340033620084782 1102 22 1.7580084785394376 1102 23 1.9451820613502662 1102 24 2.1759417083804564 1102 25 1.9555649112980689 1103 1 -1.7903562073750123 1103 2 -2.0090284488612014 1103 3 -1.8304773144603885 1103 4 -2.084215634133244 1103 5 -4.221115680011351 1103 6 -8.451768898820639 1103 7 -8.976074138426124 1103 8 -11.080601914883317 1103 9 -10.888229581996242 1103 10 -9.074737197660372 1103 11 -11.967468469987313 1103 12 -11.77637474014621 1103 13 -11.87828969500746 1103 14 -12.285430744611983 1103 15 -11.287075540552351 1103 16 -6.0058535007157055 1103 17 -4.386306417837179 1103 18 -3.66526539068359 1103 19 -3.388418652631543 1103 20 -7.074708389218976 1103 21 -4.439434508014191 1103 22 -11.292046073373687 1103 23 -12.536610709041152 1103 24 -12.846915120334994 1103 25 -14.479330129129554 1104 1 0.14817865359143667 1104 2 0.297766993489585 1104 3 0.40042253719507315 1104 4 0.5398982439717344 1104 5 0.4907487267683095 1104 6 0.6372693866952517 1104 7 0.47972234549269643 1104 8 0.7874093214263773 1104 9 1.1571638466563456 1104 10 1.0741249681698832 1104 11 0.9372501488303513 1104 12 0.9177198847314827 1104 13 0.7647744087705917 1104 14 0.9028531931464343 1104 15 0.7864844127349002 1104 16 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-0.22502852552150188 2213 19 -0.3074736089746324 2213 20 0.12763609248742114 2213 21 -0.033271179816964036 2213 22 0.08408613605862314 2213 23 -0.30067608865986983 2213 24 -0.1937533933946085 2213 25 0.11158802549747548 2214 1 -0.9654012507950682 2214 2 -0.6966445646854211 2214 3 -0.717122215986155 2214 4 -0.7305641609779201 2214 5 -0.5812626247747907 2214 6 -0.616282099198223 2214 7 -0.6899747683325472 2214 8 -0.8127283450995861 2214 9 -10.457786843757134 2214 10 -3.6465952312219763 2214 11 -1.5160341953016143 2214 12 -0.8043086751273592 2214 13 -0.6006398492273135 2214 14 -0.823482664293401 2214 15 -0.8993093133972323 2214 16 -0.8771084835501028 2214 17 -0.7176776818387154 2214 18 -1.1608412149431047 2214 19 -1.1846340032687621 2214 20 -1.0512236819360723 2214 21 -1.650229697236636 2214 22 -1.3037042524370648 2214 23 -1.6853191303068584 2214 24 -2.020859881770214 2214 25 -9.74015508007782 2215 1 -0.8023024258336129 2215 2 -0.51181689927947 2215 3 -0.44970177401072675 2215 4 -0.566660389833288 2215 5 -0.7404870579170577 2215 6 -0.7922939498993955 2215 7 -0.7426881200848854 2215 8 -0.6635229773214775 2215 9 0.1601785169316372 2215 10 -0.004846037176697848 2215 11 0.15127197282374993 2215 12 0.02768200546230304 2215 13 -0.04520479504472519 2215 14 -0.2258302940052488 2215 15 -0.708084437037023 2215 16 -0.4135969118772138 2215 17 -0.43454532507301735 2215 18 -0.17335660856394242 2215 19 -0.36229245704594604 2215 20 -0.17899900486256826 2215 21 -0.3625679381508675 2215 22 -0.27754484619850217 2215 23 0.0682602983561651 2215 24 0.22129054822918448 2215 25 0.21322500808705058 2216 1 -1.0429402895802973 2216 2 -0.9224247253561018 2216 3 -1.1779159827954295 2216 4 -0.7421309262880982 2216 5 -1.9589157027618924 2216 6 -2.4790392131572716 2216 7 -1.8326243981614567 2216 8 -2.9009788155013716 2216 9 -1.4848018339205602 2216 10 -1.8531393513781893 2216 11 -1.3219977233904174 2216 12 -2.1781720328319096 2216 13 -2.074611690988199 2216 14 -2.1447565702670097 2216 15 -1.8790749338712764 2216 16 -3.052712030272449 2216 17 -3.3247662062571557 2216 18 -2.8353863477229844 2216 19 -4.047721727746243 2216 20 -3.0424102054704463 2216 21 -3.471831277437463 2216 22 -3.180431922537604 2216 23 -3.0103603772624403 2216 24 -3.003036911560873 2216 25 -3.1461181320112885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61022.0 3 5.0 4 845.0 5 8561.0 6 11862.0 7 9475.0 8 7208.0 9 5986.0 10 5108.0 11 4595.0 12 4134.0 13 3760.0 14 3720.0 15 3854.0 16 4054.0 17 4240.0 18 4715.0 19 5997.0 20 8177.0 21 10951.0 22 13452.0 23 15744.0 24 18150.0 25 20862.0 26 23416.0 27 25519.0 28 27926.0 29 32799.0 30 39467.0 31 49037.0 32 60752.0 33 75290.0 34 93253.0 35 116049.0 36 151502.0 37 223450.0 38 508829.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14433026878708 20.032717618455575 12.58137320209412 24.241578910663225 2 15.391680085205467 22.526255557498963 39.55003867784868 22.532025679446885 3 17.91639544381675 28.524349350657936 30.300265482842786 23.258989722682525 4 10.992485342751799 18.138322107229627 39.00829605657812 31.860896493440453 5 11.268400821802663 40.338918440835684 35.343767605654975 13.048913131706682 6 28.257420969329083 39.55281525256332 19.388745722284035 12.801018055823555 7 25.860345274315556 33.563279751309196 23.447801561952375 17.128573412422877 8 25.538075463734806 36.03528911692436 21.243512101625154 17.183123317715683 9 26.489229231841332 14.961467905353606 20.811780473183862 37.7375223896212 10 13.728063977872315 31.72556860033372 34.19599236354344 20.35037505825053 11 33.18816179521253 23.737139902230187 24.219134262126524 18.855564040430764 12 21.542267833024074 28.106123857250452 31.969637680244716 18.381970629480758 13 27.92605976238819 22.95744120712475 27.38414230262027 21.732356727866794 14 20.700986682936065 22.349826135827442 30.713639546660815 26.235547634575678 15 22.754564804078736 30.355068034019112 25.587727729280086 21.302639432622065 16 20.97048954865732 29.385242009264168 28.17630772522953 21.46796071684898 17 20.161122592388214 28.862788519684678 29.357204771883254 21.61888411604385 18 19.501650405072102 27.82947838062531 32.132554187448264 20.536317026854324 19 21.158025280630387 27.195884308082963 31.459698813396198 20.186391597890456 20 21.086902680827837 26.937462579259837 31.88848198634699 20.08715275356534 21 22.2806841427267 26.226172492788102 31.00270442319564 20.49043894128955 22 21.06680516282917 27.192014349741285 31.201129609325406 20.54005087810414 23 20.70675528407441 26.783643728687434 31.36723227686909 21.142368710369066 24 20.172293473537167 27.158401921531457 31.8030045567849 20.86630004814648 25 20.64839957905352 26.735877745012093 31.637774230445935 20.977948445488455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1163.0 1 1163.0 2 2017.0 3 2871.0 4 2871.0 5 2871.0 6 8786.5 7 14702.0 8 14702.0 9 14702.0 10 14049.5 11 13397.0 12 13397.0 13 13397.0 14 13970.0 15 14543.0 16 14543.0 17 14543.0 18 23620.5 19 32698.0 20 32698.0 21 32698.0 22 46801.0 23 60904.0 24 60904.0 25 60904.0 26 83693.5 27 106483.0 28 106483.0 29 106483.0 30 122195.0 31 137907.0 32 137907.0 33 137907.0 34 150986.5 35 164066.0 36 164066.0 37 164066.0 38 173207.0 39 182348.0 40 182348.0 41 182348.0 42 190246.5 43 198145.0 44 198145.0 45 198145.0 46 201428.5 47 204712.0 48 204712.0 49 204712.0 50 194946.5 51 185181.0 52 185181.0 53 185181.0 54 162798.0 55 140415.0 56 140415.0 57 140415.0 58 117658.5 59 94902.0 60 94902.0 61 94902.0 62 76478.0 63 58054.0 64 58054.0 65 58054.0 66 43208.5 67 28363.0 68 28363.0 69 28363.0 70 20723.5 71 13084.0 72 13084.0 73 13084.0 74 9212.0 75 5340.0 76 5340.0 77 5340.0 78 3969.0 79 2598.0 80 2598.0 81 2598.0 82 1843.5 83 1089.0 84 1089.0 85 1089.0 86 790.5 87 492.0 88 492.0 89 492.0 90 391.0 91 290.0 92 290.0 93 290.0 94 153.5 95 17.0 96 17.0 97 17.0 98 9.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02656623587692019 2 0.001382405939296752 3 3.005230302819026E-4 4 0.0050487869087359645 5 0.035642031391433654 6 0.012201235029445248 7 0.020135043028887474 8 0.02866989708889351 9 0.03648349587622298 10 0.04153228278495894 11 0.04063071369411323 12 0.007513075757047565 13 0.0026446026664807432 14 0.0066716112722582386 15 0.0067918204843709995 16 0.00913590012056984 17 0.011299665938599539 18 0.014124582423249423 19 0.016769185089730167 20 0.01502615151409513 21 0.011720398180994202 22 0.010939038302261256 23 0.008114121817611371 24 0.005589728363243389 25 0.1080079770833158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1663766.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.83641593930325 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24152809942416 44.46968034785554 2 12.916123783237643 14.682123673352434 3 3.990010634798466 6.803337121249471 4 1.7304151871053053 3.934023892880175 5 0.8739852734516946 2.4837095263363107 6 0.5184251532053034 1.767925658458418 7 0.29861806114094946 1.1880666230996704 8 0.21282891632329412 0.967714624964953 9 0.1626297893352975 0.8318964915704009 >10 0.9221113806784509 9.844781483493938 >50 0.08309200318556491 3.284566814353084 >100 0.046727396172837686 4.635435828595366 >500 0.002017639905442458 0.7370008233597669 >1k 8.495325917652454E-4 0.7426138834344911 >5k 3.18574721911967E-4 1.322992224064048 >10k+ 3.18574721911967E-4 2.304130982931984 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 14794 0.8891875419980935 No Hit TATCAACGCAGAGTACTTTTTTTTT 12034 0.7232988292824832 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 11348 0.6820670695278062 No Hit GGTATCAACGCAGAGTACTTTTTTT 9882 0.5939537170491523 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 6293 0.3782382859128026 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5745 0.3453009617939061 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1960 0.11780502787050583 No Hit GTATAACGCAGAGTACTTTTTTTTT 1952 0.11732419102205478 No Hit ATCAACGCAGAGTACTTTTTTTTTT 1822 0.10951059223472531 No Hit GTGGTATCAACGCAGAGTACTTTTT 1818 0.10927017381049979 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 6.010460605638052E-5 0.0 0.0 0.0 4 0.0 6.010460605638052E-5 0.0 0.0 0.0 5 0.0 6.010460605638052E-5 0.0 0.0 0.0 6 0.0 6.010460605638052E-5 0.0 0.0 0.0 7 1.2020921211276104E-4 6.010460605638052E-5 0.0 0.0 0.0 8 1.2020921211276104E-4 6.010460605638052E-5 0.0 0.0 0.0 9 1.2020921211276104E-4 6.010460605638052E-5 0.0 0.0 0.0 10 1.2020921211276104E-4 6.010460605638052E-5 0.0 0.0 0.0 11 1.2020921211276104E-4 6.010460605638052E-5 0.0 0.0 0.0 12 1.2020921211276104E-4 6.010460605638052E-5 0.0 0.0 0.0 13 1.2020921211276104E-4 6.010460605638052E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCTG 25 0.0060275677 19.002556 9 CGACGGG 55 5.9224294E-7 17.269339 15 TATAACG 300 0.0 16.148287 2 TAACGCA 330 0.0 14.6833515 4 ATAACGC 345 0.0 14.319475 3 GCACCGT 90 3.6328856E-8 13.724068 6 AATGCGT 80 2.0047282E-6 13.059545 12 TCACGCA 165 0.0 12.66799 4 GTATAAC 490 0.0 12.601084 1 TGCACCG 70 1.08839726E-4 12.217398 5 ACGCATC 55 0.0030675833 12.091444 10 TATCACG 175 0.0 11.941591 2 CCGACGG 80 2.8724056E-5 11.87267 14 GGTATCA 2770 0.0 11.694058 1 CGAGACG 65 8.0391864E-4 11.689663 18 GAATGCG 90 7.4651434E-6 11.610579 11 ACGGGCG 90 7.4801465E-6 11.608484 17 GGGCGCT 100 1.9126437E-6 11.408395 19 ATCACGC 175 1.8189894E-12 11.400505 3 CAGTCCG 70 0.0014898248 10.8586035 9 >>END_MODULE