FastQCFastQC Report
Thu 26 May 2016
SRR1364291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380229
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92110.6673530262007246No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63280.45847464442494684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA59970.43449311672193525No Hit
TATCAACGCAGAGTACTTTTTTTTT56140.4067440982619551No Hit
GGTATCAACGCAGAGTACTTTTTTT40730.29509595871409744No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN37300.27024501006717No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA26400.19127260766148227No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25940.18793982737647158No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18450.13367347012705863No Hit
GTGGTATCAACGCAGAGTACATGGG14110.10222941265543616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1700.016.1950592
ATAACGC2450.015.88734153
TATAACG2550.014.8920072
CGGGACC456.736238E-414.785114
TAACGCA2650.014.7054064
AGACGAA602.5672769E-514.25032710
GTATCAC2300.013.6208341
CTCAACG1051.3842509E-913.5623653
GTATAAC4300.013.0257031
ATCACGC2150.012.8053943
CGCATAT905.3995427E-712.6674188
CTGGTCG604.0906967E-412.6669589
GGTATCA20250.012.4233441
AAAGCCG550.003054255412.0982319
GTATCGA550.003084366712.0823951
CGAAATC802.8994455E-511.86146213
TCACGCA2300.011.5709564
TGGTCGG752.0730396E-411.40026210
GTTATCA3050.011.2052181
CACGCAG2550.011.1820165
Produced by FastQC (version 0.11.5)