##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364291_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1380229
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.498420914210612	30.0	25.0	31.0	2.0	34.0
2	25.42220240264478	30.0	25.0	31.0	2.0	34.0
3	24.862459780224874	30.0	22.0	31.0	2.0	34.0
4	28.542707043541327	35.0	27.0	37.0	2.0	37.0
5	29.13638896154189	35.0	28.0	37.0	2.0	37.0
6	29.206835242557574	35.0	29.0	37.0	2.0	37.0
7	28.79152082734097	35.0	28.0	37.0	2.0	37.0
8	28.253485472338287	35.0	26.0	37.0	2.0	37.0
9	29.19141171501251	35.0	27.0	39.0	2.0	39.0
10	28.537293449130544	35.0	23.0	39.0	2.0	39.0
11	29.143821061577462	35.0	27.0	39.0	2.0	39.0
12	28.690702050167037	35.0	25.0	39.0	2.0	39.0
13	28.32093877175454	35.0	23.0	39.0	2.0	39.0
14	28.569817037607528	35.0	18.0	40.0	2.0	41.0
15	29.23781995596383	36.0	25.0	40.0	2.0	41.0
16	29.576834713659835	36.0	27.0	40.0	2.0	41.0
17	29.750242894476205	36.0	27.0	40.0	2.0	41.0
18	29.59426950165516	36.0	27.0	40.0	2.0	41.0
19	29.59255094625602	37.0	27.0	40.0	2.0	41.0
20	29.458392773952728	37.0	26.0	40.0	2.0	41.0
21	29.371239120464793	37.0	25.0	40.0	2.0	41.0
22	29.445121787761305	37.0	27.0	40.0	2.0	41.0
23	29.436582625057145	37.0	27.0	40.0	2.0	41.0
24	29.30637741997886	37.0	26.0	40.0	2.0	41.0
25	29.128078746352962	37.0	25.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4648337836657852
1101	2	0.6052519244939845
1101	3	1.3983053548498532
1101	4	0.7938566049980516
1101	5	0.2196355388457505
1101	6	0.04795505747740236
1101	7	0.170309808511238
1101	8	0.38361027380270585
1101	9	0.33611533354339684
1101	10	0.5725401966554458
1101	11	-0.042210650393528226
1101	12	0.3889394704863065
1101	13	0.2787156490069478
1101	14	0.8672464932062667
1101	15	0.10522461967253349
1101	16	-0.4037221836618663
1101	17	-0.9121128068275297
1101	18	-6.314182471452124
1101	19	-7.686482342468839
1101	20	-8.58351895462291
1101	21	-10.417215377493662
1101	22	-10.6911283392167
1101	23	-12.175367066023504
1101	24	-11.63637682587397
1101	25	-12.128411860918234
1102	1	1.6380258988029475
1102	2	1.889883794824108
1102	3	2.5148490773442056
1102	4	1.9976076457523675
1102	5	1.5436279264713164
1102	6	1.385067761708541
1102	7	1.6409297196281187
1102	8	1.9302507729322578
1102	9	1.9184325662722799
1102	10	-1.2825744755474915
1102	11	0.7551821113629416
1102	12	1.6678469217465626
1102	13	2.146146148244629
1102	14	2.6112643995870357
1102	15	2.2237274472803676
1102	16	2.071232671930094
1102	17	1.677269660922601
1102	18	1.8765107520208062
1102	19	1.770395070188897
1102	20	2.1151257399630055
1102	21	2.1195348739093305
1102	22	2.103577167860429
1102	23	2.114037244050998
1102	24	2.1961200113142105
1102	25	2.20976830581872
1103	1	-2.8080345868043928
1103	2	-2.5310894073356174
1103	3	-4.532503269537109
1103	4	-3.274425213898496
1103	5	-5.21304370889256
1103	6	-9.095467532603955
1103	7	-9.802894189742844
1103	8	-4.527625937127699
1103	9	-9.65904002308146
1103	10	-7.435483150892818
1103	11	-10.44882994600895
1103	12	-4.966031643293682
1103	13	-2.8318246358157886
1103	14	-2.3427214893128934
1103	15	-2.9369416706102562
1103	16	-2.8563303477128237
1103	17	-2.850436457199894
1103	18	-2.718937921337041
1103	19	-7.974913485907695
1103	20	-4.076395675121958
1103	21	-8.849619453825937
1103	22	-5.8066894855563795
1103	23	-4.844974316451172
1103	24	-10.013829307974273
1103	25	-6.994198702031824
1104	1	1.584187568647991
1104	2	1.7296339016383335
1104	3	2.518262130563741
1104	4	1.9261921748839939
1104	5	1.6219582898481022
1104	6	1.477614525497998
1104	7	1.6223502967375687
1104	8	2.144257921669766
1104	9	2.2572506277764326
1104	10	2.3847503751731125
1104	11	2.015797569726235
1104	12	2.2403363705851547
1104	13	2.3586174647169464
1104	14	2.8610677572010665
1104	15	1.8564734586808314
1104	16	1.2200973148579486
1104	17	0.9111294581318852
1104	18	0.9799746592331111
1104	19	1.4250841626327002
1104	20	-9.119273234620419
1104	21	-3.326705786358904
1104	22	-0.968472518991522
1104	23	0.18470241010804145
1104	24	0.8505646294403242
1104	25	1.2798286428306298
1105	1	0.8965150904211328
1105	2	0.9274203351958974
1105	3	1.7062048946344603
1105	4	1.0068188152019388
1105	5	0.8789460287370545
1105	6	0.7591093554521358
1105	7	1.1853662804972025
1105	8	1.4679913402343416
1105	9	1.4372784108397099
1105	10	1.9672068340720479
1105	11	1.3734839892305928
1105	12	1.6148201018231916
1105	13	1.688729753561315
1105	14	2.3042123837568163
1105	15	1.5497750956098386
1105	16	1.09389952780257
1105	17	0.612838145541744
1105	18	1.0202913994200138
1105	19	0.9868490379923749
1105	20	1.019531397216685
1105	21	0.9930413305575492
1105	22	0.8694620991944895
1105	23	0.809018559977396
1105	24	0.8329691039146851
1105	25	0.8244361707888395
1106	1	0.8907281539634972
1106	2	1.2426107537285134
1106	3	1.7232884803550412
1106	4	1.419382539628753
1106	5	0.6292297431134415
1106	6	0.5611480650575729
1106	7	1.0663152436657235
1106	8	1.0503157146519193
1106	9	1.3556491339621566
1106	10	-1.9167038303914197
1106	11	-0.012296111046268265
1106	12	0.6130473295201533
1106	13	1.5253212158023572
1106	14	0.6645649384307077
1106	15	1.22814974106074
1106	16	0.7768018019591665
1106	17	-5.344624760238474
1106	18	-1.6707958387007267
1106	19	-0.4756917596950814
1106	20	0.295590796031874
1106	21	0.5331208542649968
1106	22	0.5242100799355995
1106	23	0.1406029382100158
1106	24	0.47381770868795314
1106	25	0.6284974114676771
1107	1	0.8886360758081082
1107	2	1.1843158900712503
1107	3	1.6394467784936282
1107	4	1.0425048940978918
1107	5	0.6594842958073066
1107	6	0.6650297641783816
1107	7	0.7246857754844882
1107	8	0.9155628276487029
1107	9	0.9475551399716551
1107	10	-2.0664046265876728
1107	11	-0.17316657930549795
1107	12	0.2787319475532186
1107	13	1.0391248379631328
1107	14	1.4200886926746534
1107	15	1.0887488208910838
1107	16	-5.331708970196502
1107	17	-2.056879986017016
1107	18	-0.3255727792163299
1107	19	0.44915825058216896
1107	20	0.9083919443263895
1107	21	1.1342684162290766
1107	22	0.7246733966055601
1107	23	0.6331974986344484
1107	24	0.6847321515250968
1107	25	0.906999552454355
1108	1	-0.729059532603344
1108	2	-3.3048180132367406
1108	3	-0.4563634494792588
1108	4	-0.9356407023471469
1108	5	-1.2192628462569246
1108	6	-0.8193884390265822
1108	7	-0.3890498839157672
1108	8	-0.1478617050246953
1108	9	-0.7680613679066575
1108	10	-3.4472382527162075
1108	11	-1.867680191311969
1108	12	-6.2522984030032625
1108	13	-2.1255703410153615
1108	14	-6.03596486932749
1108	15	-2.9904102712910294
1108	16	-7.592865316044495
1108	17	-3.4851213986308025
1108	18	-2.2033541214574264
1108	19	-0.8967041713178929
1108	20	-0.8947435952400014
1108	21	-0.3535067476089324
1108	22	-0.431731434769862
1108	23	-0.3865852727867036
1108	24	-0.21518153754167813
1108	25	-0.5459720217993578
1109	1	-8.07393767212724
1109	2	-6.017864622158637
1109	3	-5.004703533388312
1109	4	-4.7593405802715445
1109	5	-2.158735871091338
1109	6	-1.298468544077405
1109	7	-0.8465598537047967
1109	8	-0.35224512197742897
1109	9	-5.143508184028061
1109	10	-1.1757607076254288
1109	11	-0.8574902106230304
1109	12	-5.652840530054693
1109	13	-7.329356456454846
1109	14	-7.3075080079107195
1109	15	-3.236448297800081
1109	16	-1.6492466137579598
1109	17	-1.3137005043826981
1109	18	-0.8332423130258597
1109	19	-0.5201055672149018
1109	20	-0.3343279813734945
1109	21	-0.2026263184292354
1109	22	-0.4489922252157399
1109	23	-0.6941634935537309
1109	24	-0.48680165884112725
1109	25	-0.2677739910383288
1110	1	-2.4180814363179195
1110	2	-2.240751402947712
1110	3	-1.4533802257257804
1110	4	-2.4870081385796965
1110	5	-5.4403381104017505
1110	6	-4.089311694133158
1110	7	-8.41519362014865
1110	8	-9.26725416356875
1110	9	-10.98559520284497
1110	10	-8.907007349526417
1110	11	-11.415237958891264
1110	12	-6.6052421846947915
1110	13	-4.968443862355855
1110	14	-4.639049873656088
1110	15	-9.396132044311994
1110	16	-10.904725009265473
1110	17	-11.768056355996606
1110	18	-8.709852034036437
1110	19	-11.61344916501719
1110	20	-12.693019174921325
1110	21	-13.538343781699576
1110	22	-14.01823406034102
1110	23	-14.99656428158763
1110	24	-15.244160589795724
1110	25	-16.085578704220566
1111	1	-0.45013538931158337
1111	2	0.21102974498232996
1111	3	-2.339328607061418
1111	4	-2.981428901022344
1111	5	-0.8715986150907042
1111	6	-0.257311543370939
1111	7	0.13999301436645695
1111	8	0.5802854715701145
1111	9	-3.5244699037020766
1111	10	-3.755998899386878
1111	11	-1.0158589004261707
1111	12	-5.179754133429011
1111	13	-0.9408502344618626
1111	14	0.2873912102082947
1111	15	0.4011793351705961
1111	16	0.25662659887866823
1111	17	0.2903673173165764
1111	18	0.6309612867168113
1111	19	0.6692757626873558
1111	20	0.7297035907535978
1111	21	0.8536495847627883
1111	22	0.5372282122420415
1111	23	0.44322464302214826
1111	24	0.5510715964469739
1111	25	0.730543575536128
1112	1	-0.06680765291053703
1112	2	0.610050189847172
1112	3	-1.9774824769898416
1112	4	-0.008936301921615097
1112	5	-0.09101803136533704
1112	6	0.09639412818239634
1112	7	0.44272895902602727
1112	8	-2.9488292607302427
1112	9	-0.5687920883142361
1112	10	-2.7436348443436316
1112	11	-0.6437924606652246
1112	12	0.5574624661523018
1112	13	1.0818228603982867
1112	14	1.790206587436579
1112	15	1.0803413831298663
1112	16	0.9077042878366903
1112	17	0.5653797671299223
1112	18	0.7668945684536439
1112	19	0.7526493925179985
1112	20	0.754687914390157
1112	21	0.7864295081492045
1112	22	0.778784309424033
1112	23	0.5538846975253726
1112	24	0.6796922844918001
1112	25	0.7400920410780572
1113	1	-0.8095181398286222
1113	2	-0.36227698868946234
1113	3	-2.963117324070474
1113	4	-1.1791794045094832
1113	5	-1.2756144050256566
1113	6	-1.065674263728443
1113	7	-1.0857043006398541
1113	8	-8.139509140010677
1113	9	-8.597356048296174
1113	10	-3.0612128486686245
1113	11	-8.072121160078261
1113	12	-6.791162804159715
1113	13	-2.27026002736579
1113	14	-0.5935414223540647
1113	15	-6.560696139742689
1113	16	-2.436786099749156
1113	17	-1.568825693766918
1113	18	-0.891359750597708
1113	19	-0.6684432621242387
1113	20	-5.989388984923071
1113	21	-2.0527496938659553
1113	22	-1.266556818400879
1113	23	-1.1758151670215753
1113	24	-6.491584538777609
1113	25	-3.6200705025877298
1114	1	-4.207967855020421
1114	2	-4.191350629980295
1114	3	-0.9669957985371838
1114	4	-0.7211823990011652
1114	5	-0.8072996629552591
1114	6	-1.031986232302856
1114	7	-0.7636583224852806
1114	8	-0.37652149919498257
1114	9	-0.5809692174297076
1114	10	-3.307783271709635
1114	11	-1.3356778648114371
1114	12	-5.049045444395297
1114	13	-1.6428283840970401
1114	14	-6.345230658206194
1114	15	-2.426217496214271
1114	16	-1.5671072617445247
1114	17	-1.4082778454362632
1114	18	-0.7773093967283486
1114	19	-0.635677887729905
1114	20	-0.3340207232199681
1114	21	-0.1409628884139913
1114	22	-0.2354627239583671
1114	23	-0.6917710562806292
1114	24	-0.7189918725197835
1114	25	-0.5317191857296244
1115	1	0.39615917069706796
1115	2	0.41105933177723486
1115	3	1.2918595476101835
1115	4	0.10125047912410068
1115	5	-0.08570213010396088
1115	6	-0.5899868935918704
1115	7	-2.3731091406744724
1115	8	-0.36586268481548245
1115	9	-0.22944752486255382
1115	10	0.4282280741798701
1115	11	-0.4948611520832884
1115	12	0.35671567222655653
1115	13	0.4568411793482312
1115	14	1.0803589391769286
1115	15	0.27631224619494077
1115	16	-0.10270686164387755
1115	17	-0.3400213817520523
1115	18	-0.06983745472214764
1115	19	-0.013237523313907928
1115	20	0.2756848368065654
1115	21	0.09982824615416419
1115	22	-0.40030835040493784
1115	23	-0.2425815512284224
1115	24	-0.2616666410166424
1115	25	-0.18802295445314954
1116	1	0.2252966429928982
1116	2	0.44077229218126845
1116	3	1.0409066325082286
1116	4	0.38619729253481694
1116	5	-0.1874703200258736
1116	6	-1.1868493454313196
1116	7	-4.283073971956746
1116	8	-0.9872121014699573
1116	9	-1.05098768710468
1116	10	-0.36078237889508813
1116	11	-2.080468530469428
1116	12	-0.3347338316723487
1116	13	-6.166896892278903
1116	14	-1.4567513735482933
1116	15	-1.2768380364011485
1116	16	-0.7758350350923067
1116	17	-0.41123776419695446
1116	18	-0.2619236803980236
1116	19	0.055627052737769134
1116	20	0.10619546057715112
1116	21	0.37667053965309805
1116	22	0.1279550519111048
1116	23	-0.12111671181778405
1116	24	0.1582175027144963
1116	25	0.27118621000531107
1201	1	2.381481694269702
1201	2	2.476556906866218
1201	3	3.122935875066837
1201	4	2.7523557389954654
1201	5	2.3321433416609167
1201	6	2.099786850762147
1201	7	2.352966458268142
1201	8	2.534871032895733
1201	9	2.9389911654773506
1201	10	3.1310349347006543
1201	11	2.6573660194089186
1201	12	3.2051108598709597
1201	13	3.1768680302942585
1201	14	3.8895080347787854
1201	15	3.2664721029898978
1201	16	2.750553759050085
1201	17	2.6115730232601244
1201	18	2.5598172296231283
1201	19	2.5973238487902464
1201	20	2.764084811828944
1201	21	2.7254323924397816
1201	22	1.8547974922776618
1201	23	1.612076775687047
1201	24	2.0014310006793394
1201	25	2.0709929271529113
1202	1	2.2283154109544654
1202	2	2.6366059872503094
1202	3	3.2342927681246145
1202	4	2.7988653883357877
1202	5	2.0554740528134516
1202	6	1.9602933876878659
1202	7	2.225502392120781
1202	8	2.43804372915314
1202	9	2.6455930756790593
1202	10	-0.624599903821462
1202	11	1.359882030387876
1202	12	2.2173411956526117
1202	13	2.7893275218762064
1202	14	3.265246164422827
1202	15	2.8094520689585494
1202	16	2.4898548338591944
1202	17	2.0301581572051113
1202	18	2.1609698490962366
1202	19	2.5840285370785097
1202	20	2.629927771701027
1202	21	2.6980628103070963
1202	22	2.4061425000159424
1202	23	1.9559160759067993
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2211	16	-0.026926745093419413
2211	17	0.5364778346135353
2211	18	0.8440413657988834
2211	19	1.2025729681680097
2211	20	1.305692940023107
2211	21	1.4919035915113135
2211	22	1.1906810668913081
2211	23	1.074055274251581
2211	24	1.4675316257684194
2211	25	1.5042285266886743
2212	1	0.7660708771825675
2212	2	0.7231713908175976
2212	3	1.379093462536492
2212	4	0.8931837065490775
2212	5	0.27278617966443974
2212	6	0.5068704919680869
2212	7	0.5564274876858768
2212	8	0.8987167745100955
2212	9	0.6548181180663235
2212	10	1.5550851307918236
2212	11	0.6260454286183013
2212	12	1.2530573834140775
2212	13	1.09087007646297
2212	14	-4.325362821120592
2212	15	-1.0445751781228303
2212	16	-0.4352943860005425
2212	17	-0.06573602129276424
2212	18	0.16444628034699704
2212	19	0.4529853215445385
2212	20	0.9523964790504564
2212	21	0.8942794738074689
2212	22	0.756045086811465
2212	23	0.6371825350023919
2212	24	0.6422609981622287
2212	25	0.7578925755691621
2213	1	0.4682951063044598
2213	2	0.572584944249396
2213	3	1.1719029188445056
2213	4	0.599588891971365
2213	5	0.08166785387603426
2213	6	-0.20857107676695819
2213	7	0.09256311345751911
2213	8	-5.361267678368407
2213	9	-1.9302455946472605
2213	10	-0.24501851668481933
2213	11	-0.35447608585424106
2213	12	0.3023932012383952
2213	13	-8.245184765245991
2213	14	-2.630983029330082
2213	15	-1.327334258708742
2213	16	-0.7867679330305819
2213	17	-0.7289705084663893
2213	18	-0.42911538228046453
2213	19	0.0805342111447338
2213	20	0.3579690551475174
2213	21	0.40029712436304976
2213	22	-0.007753745630193265
2213	23	-0.14579336695928902
2213	24	-0.16795742365251698
2213	25	0.015004240482966225
2214	1	-0.7469997798470871
2214	2	-0.45081931106975404
2214	3	-6.5041702427829655
2214	4	-2.1866886369573386
2214	5	-1.2192046731863044
2214	6	-0.7126957501525482
2214	7	-0.4187517428321428
2214	8	-5.914935091582294
2214	9	-1.719607296774921
2214	10	0.02242735864179579
2214	11	-0.22685383874711107
2214	12	-0.060120796521960784
2214	13	-5.604959867896234
2214	14	-1.3064122939810723
2214	15	-0.5783794248154983
2214	16	-0.4205165332545491
2214	17	-0.26971798886952314
2214	18	-0.3848917913883412
2214	19	-7.274536555641475E-4
2214	20	0.25112415033228785
2214	21	0.33204404485577044
2214	22	0.1821165417904851
2214	23	0.140073560998907
2214	24	0.36603380441765765
2214	25	0.41339449804175743
2215	1	0.3022209859029097
2215	2	0.4825940850172188
2215	3	-2.4100657138890647
2215	4	-0.5642112619784108
2215	5	-0.2046418417524336
2215	6	-0.3678505597522985
2215	7	0.19325588744000655
2215	8	0.540416147283473
2215	9	0.6747665930431737
2215	10	1.1448528711082488
2215	11	0.516872417946395
2215	12	0.27381867992597364
2215	13	0.8912403927128913
2215	14	1.0995415224515632
2215	15	0.587867479640785
2215	16	0.43276684999645454
2215	17	0.15065279568063517
2215	18	0.27827901779651043
2215	19	0.2887528839693907
2215	20	0.7579562259996955
2215	21	0.825684404262983
2215	22	0.6271479510246074
2215	23	0.5850158315397032
2215	24	0.3522083930831066
2215	25	0.382133558293031
2216	1	-0.002726155201145275
2216	2	-0.16361008018418843
2216	3	0.9862556042007213
2216	4	-0.5718056928915018
2216	5	-0.6901240203007468
2216	6	-0.9372259825787985
2216	7	-1.5432887886210906
2216	8	-6.465908890461279
2216	9	-2.28997830603479
2216	10	-1.0904875869615758
2216	11	-1.6349707198180923
2216	12	-0.5842519155518033
2216	13	0.06677054506443625
2216	14	0.6505162263613435
2216	15	0.14380118207001402
2216	16	-0.36755366568741366
2216	17	-0.38304764377467393
2216	18	-0.2731238931177522
2216	19	0.10110908235207106
2216	20	0.10753922668921234
2216	21	-0.07818261563554785
2216	22	-0.31344984950605337
2216	23	-0.5728339804877791
2216	24	-0.5077167508910954
2216	25	-0.43123661153601844
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	153468.0
3	5.0
4	971.0
5	7222.0
6	11838.0
7	10402.0
8	8808.0
9	6956.0
10	6080.0
11	5605.0
12	5343.0
13	5055.0
14	4985.0
15	5008.0
16	4913.0
17	5194.0
18	6074.0
19	7863.0
20	10937.0
21	13755.0
22	16994.0
23	19461.0
24	21682.0
25	24024.0
26	26295.0
27	28591.0
28	30998.0
29	34786.0
30	40060.0
31	44188.0
32	54452.0
33	68109.0
34	85525.0
35	105876.0
36	134471.0
37	158428.0
38	205807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.33313246750226	18.771820534981547	13.497384261665424	21.39766273585077
2	13.666616267642082	22.552351986787702	42.96955414309058	20.811477602479634
3	15.695682771215372	31.32120984767854	30.24357475616352	22.739532624942566
4	9.857397828433921	18.865071373786353	40.03802239904617	31.239508398733555
5	11.071034143298467	40.96214289713096	33.956226838707614	14.010596120862958
6	26.06971912810761	39.962751938519624	20.474952749605972	13.492576183766792
7	25.25087658739531	35.46841560234726	22.744908526061444	16.535799284195992
8	24.38878872506593	36.13019310664577	21.64592294483093	17.835095223457373
9	26.556145955357636	18.801213872328304	20.57994668961087	34.062693482703196
10	12.884055071567577	32.90093301565938	33.25275085026281	20.962261062510233
11	30.864063855731626	27.470202063509824	23.77018564030864	17.89554844044991
12	21.76793580708407	30.085633187074794	30.528042252681853	17.618388753159287
13	26.147197974714448	26.06552385879679	27.09829450785169	20.688983658637074
14	20.029750355710775	25.163685090958772	30.402480019882017	24.40408453344844
15	22.046839588469652	31.94846206554287	25.468871822930634	20.535826523056848
16	20.28832591772269	32.03587397022565	26.970296137560485	20.705503974491172
17	19.910016479540086	31.060154680974122	27.56253941865298	21.467289420832813
18	19.082635470217443	29.890097912768628	30.870242874529964	20.157023742483968
19	20.664473698551898	29.362883905663136	30.879359868392847	19.09328252739212
20	21.400503976404412	28.422900419883263	30.7308385943121	19.44575700940023
21	22.251658901390194	28.270862338070007	30.109314553281035	19.368164207258765
22	21.492423218508073	28.45365933767289	30.14726292674349	19.906654517075552
23	21.021390875580657	28.827073165237778	30.00971486825345	20.141821090928115
24	20.34733323644819	28.83062054933876	30.744441214939688	20.077604999273362
25	20.556628491461804	28.590060496146876	30.805733691979718	20.047577320411598
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10571.0
1	10571.0
2	9625.0
3	8679.0
4	8679.0
5	8679.0
6	12245.5
7	15812.0
8	15812.0
9	15812.0
10	14874.0
11	13936.0
12	13936.0
13	13936.0
14	15524.0
15	17112.0
16	17112.0
17	17112.0
18	24559.5
19	32007.0
20	32007.0
21	32007.0
22	43752.5
23	55498.0
24	55498.0
25	55498.0
26	73504.0
27	91510.0
28	91510.0
29	91510.0
30	106275.5
31	121041.0
32	121041.0
33	121041.0
34	129042.5
35	137044.0
36	137044.0
37	137044.0
38	143669.0
39	150294.0
40	150294.0
41	150294.0
42	155429.5
43	160565.0
44	160565.0
45	160565.0
46	162225.5
47	163886.0
48	163886.0
49	163886.0
50	154397.0
51	144908.0
52	144908.0
53	144908.0
54	125866.5
55	106825.0
56	106825.0
57	106825.0
58	88839.5
59	70854.0
60	70854.0
61	70854.0
62	56087.5
63	41321.0
64	41321.0
65	41321.0
66	31378.5
67	21436.0
68	21436.0
69	21436.0
70	15658.0
71	9880.0
72	9880.0
73	9880.0
74	6979.0
75	4078.0
76	4078.0
77	4078.0
78	2976.5
79	1875.0
80	1875.0
81	1875.0
82	1244.0
83	613.0
84	613.0
85	613.0
86	462.0
87	311.0
88	311.0
89	311.0
90	226.0
91	141.0
92	141.0
93	141.0
94	80.5
95	20.0
96	20.0
97	20.0
98	16.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32733698538430944
2	0.32900337552681475
3	0.3417548827042469
4	0.34226204492153117
5	0.34921741247285776
6	0.2934295685715921
7	0.3009645500855293
8	0.30067474310422404
9	0.29973287041498187
10	0.4096421680750078
11	0.2990083529617187
12	0.2990083529617187
13	0.2918356301744131
14	0.2978491250364976
15	0.28980698130527616
16	0.29545821744072903
17	0.28814059116277085
18	0.2900967882865814
19	0.290676402249192
20	0.4891941844433061
21	0.2910386609758236
22	0.2935020203169184
23	0.2889375603613603
24	0.2919080819197394
25	0.285677231821676
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1380229.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.80144762740636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.93365759735151	63.85565358289925
2	6.907090506662884	9.918781945438216
3	1.8464685703950081	3.9773734905860723
4	0.7438330924531286	2.1363317132522015
5	0.37781511313841903	1.3563836029425402
6	0.23546794747641167	1.0144163699196267
7	0.1573501082455375	0.7908575889451078
8	0.11932936165521033	0.6854416729038734
9	0.0896605306507434	0.5793980306188334
>10	0.5234654387206928	7.006264673264356
>50	0.043204492706525266	2.143711651861165
>100	0.02059752547524186	2.692006749323766
>500	7.209002740224755E-4	0.3743848605643375
>1k	9.2687178088604E-4	1.4613802092302988
>5k	4.1194301372712887E-4	2.0076138582503975
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9211	0.6673530262007246	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6328	0.45847464442494684	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5997	0.43449311672193525	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5614	0.4067440982619551	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4073	0.29509595871409744	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3730	0.27024501006717	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2640	0.19127260766148227	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2594	0.18793982737647158	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1845	0.13367347012705863	No Hit
GTGGTATCAACGCAGAGTACATGGG	1411	0.10222941265543616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.245174532631904E-5	0.0	0.0	0.0	0.0
8	7.245174532631904E-5	0.0	0.0	0.0	0.0
9	7.245174532631904E-5	0.0	0.0	7.245174532631904E-5	0.0
10	1.4490349065263808E-4	0.0	0.0	7.245174532631904E-5	0.0
11	1.4490349065263808E-4	0.0	0.0	7.245174532631904E-5	0.0
12	1.4490349065263808E-4	0.0	0.0	7.245174532631904E-5	0.0
13	1.4490349065263808E-4	0.0	0.0	7.245174532631904E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	170	0.0	16.195059	2
ATAACGC	245	0.0	15.8873415	3
TATAACG	255	0.0	14.892007	2
CGGGACC	45	6.736238E-4	14.78511	4
TAACGCA	265	0.0	14.705406	4
AGACGAA	60	2.5672769E-5	14.250327	10
GTATCAC	230	0.0	13.620834	1
CTCAACG	105	1.3842509E-9	13.562365	3
GTATAAC	430	0.0	13.025703	1
ATCACGC	215	0.0	12.805394	3
CGCATAT	90	5.3995427E-7	12.667418	8
CTGGTCG	60	4.0906967E-4	12.666958	9
GGTATCA	2025	0.0	12.423344	1
AAAGCCG	55	0.0030542554	12.09823	19
GTATCGA	55	0.0030843667	12.082395	1
CGAAATC	80	2.8994455E-5	11.861462	13
TCACGCA	230	0.0	11.570956	4
TGGTCGG	75	2.0730396E-4	11.400262	10
GTTATCA	305	0.0	11.205218	1
CACGCAG	255	0.0	11.182016	5
>>END_MODULE