FastQCFastQC Report
Thu 26 May 2016
SRR1364291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380229
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT201011.456352532804339No Hit
GTACTTTTTTTTTTTTTTTTTTTTT169091.2250865617227287No Hit
TATCAACGCAGAGTACTTTTTTTTT148051.0726480895561532No Hit
GGTATCAACGCAGAGTACTTTTTTT140431.0174398596174983No Hit
GAGTACTTTTTTTTTTTTTTTTTTT81580.5910613383721107No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57810.41884353973145033No Hit
GTGGTATCAACGCAGAGTACTTTTT33930.24582877189220048No Hit
GTATAACGCAGAGTACTTTTTTTTT25450.18438969185548196No Hit
ATCAACGCAGAGTACTTTTTTTTTT23860.17286986434859722No Hit
GTGGTATCAACGCAGAGTACATGGG21750.1575825460847439No Hit
GCAGAGTACTTTTTTTTTTTTTTTT21360.1547569280170175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT307.7106385E-419.0042068
TAACGCA3550.017.1311744
ATAACGC3550.016.5934183
TATAACG3450.016.5224022
GTTCCGT400.00527095914.2531556
TATCACG1500.013.9338912
GTATAAC5100.013.4118161
GGTATCA35400.012.9619141
GTACCGT604.0838734E-412.6694716
CAACCGT550.00306306212.0935866
GTGGTAT11550.011.9264531
TAAACGC1052.7097303E-711.76493554
GTATCAC1950.011.6923521
CCTATAC1157.05877E-811.566523
AAACGCA1251.8177161E-811.4054185
ACAACGC752.071326E-411.4012833
GTTTAAG1104.9657865E-711.2273161
TGGTATC11400.011.1671142
GAGTTAC951.3597188E-511.0000421
TAAGGCC700.001488016910.8599414
Produced by FastQC (version 0.11.5)