##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364291_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1380229
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.471812286222068	31.0	30.0	34.0	26.0	34.0
2	29.479671127037616	31.0	30.0	34.0	25.0	34.0
3	29.52627426318386	31.0	30.0	34.0	25.0	34.0
4	32.83116932045335	35.0	33.0	37.0	26.0	37.0
5	32.43968935589674	35.0	32.0	37.0	25.0	37.0
6	32.29636169070495	35.0	32.0	37.0	23.0	37.0
7	32.01884687251174	35.0	32.0	37.0	19.0	37.0
8	31.883031004275377	35.0	32.0	37.0	17.0	37.0
9	32.57430035160832	37.0	32.0	39.0	11.0	39.0
10	32.732129958144625	37.0	32.0	39.0	17.0	39.0
11	32.6392388509443	37.0	32.0	39.0	13.0	39.0
12	32.58196719529875	37.0	32.0	39.0	13.0	39.0
13	32.62742414483394	37.0	32.0	39.0	13.0	39.0
14	33.51958189546807	38.0	32.0	41.0	12.0	41.0
15	33.55511947655063	38.0	32.0	41.0	13.0	41.0
16	33.520517972017686	38.0	32.0	41.0	11.0	41.0
17	33.54270414547151	38.0	32.0	41.0	11.0	41.0
18	33.52206409226295	38.0	32.0	41.0	10.0	41.0
19	33.45722702537043	39.0	32.0	41.0	10.0	41.0
20	33.3409122689061	39.0	32.0	41.0	10.0	41.0
21	33.277432947720996	39.0	32.0	41.0	2.0	41.0
22	33.21997146850269	39.0	32.0	41.0	2.0	41.0
23	32.93764005828018	38.0	32.0	41.0	2.0	41.0
24	32.86199681357224	38.0	32.0	41.0	2.0	41.0
25	32.713958335899335	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.538261061774179
1101	2	0.6710612486810774
1101	3	-1.1888236110624675
1101	4	-0.13893590131623768
1101	5	0.6189669369450783
1101	6	0.9360738618594056
1101	7	1.1185066177399783
1101	8	1.3183100380947081
1101	9	1.9338068569974851
1101	10	1.7927125882845232
1101	11	1.5218880150116547
1101	12	1.6895842891283763
1101	13	1.511868101404346
1101	14	1.7103734694076707
1101	15	1.4113838538872798
1101	16	1.4025638468739388
1101	17	1.4524884112186172
1101	18	1.5112587861526805
1101	19	1.6241030759789012
1101	20	1.7059159920424918
1101	21	1.7699004780588439
1101	22	1.836876427211699
1101	23	2.0447410457194977
1101	24	2.166704834268856
1101	25	2.1924603165191883
1102	1	0.6075574577906586
1102	2	0.603300735710846
1102	3	0.8675060134009378
1102	4	0.6796564581954456
1102	5	0.8053881477883849
1102	6	0.7726346689441073
1102	7	0.9258229313611572
1102	8	1.0304197687420853
1102	9	1.7226315885639707
1102	10	1.1373857643963774
1102	11	1.2244256173299704
1102	12	1.4406179068444445
1102	13	1.1507902583879428
1102	14	1.4298142193995602
1102	15	1.330014707792408
1102	16	1.373379473867594
1102	17	1.4298779290655261
1102	18	1.3473440360959117
1102	19	1.344808950699118
1102	20	1.6427045215782883
1102	21	1.8401642079060565
1102	22	1.688342566202408
1102	23	2.103909739829639
1102	24	1.9735274248550283
1102	25	1.7753013325116598
1103	1	-3.41776302317923
1103	2	-3.3232075951381397
1103	3	-3.3141323316916456
1103	4	-3.315956513416733
1103	5	-6.160674987790799
1103	6	-10.189706581180111
1103	7	-11.98366330534758
1103	8	-12.491898956621462
1103	9	-13.538066016523974
1103	10	-11.620893078890916
1103	11	-13.413612078519513
1103	12	-12.651599788342544
1103	13	-13.34412643312221
1103	14	-13.942221952716107
1103	15	-14.559170094619066
1103	16	-8.932857897360336
1103	17	-7.437101434278738
1103	18	-6.814834240759897
1103	19	-6.638277604006241
1103	20	-10.266340494818415
1103	21	-7.735199003859819
1103	22	-13.020338079065734
1103	23	-13.314425415359338
1103	24	-14.276147622347594
1103	25	-14.70492921503877
1104	1	0.21570451753368047
1104	2	0.4851366899005676
1104	3	0.5205992491946425
1104	4	0.5279287771583512
1104	5	0.6756425219612296
1104	6	0.7213494593276977
1104	7	0.6552805235079653
1104	8	0.8644281679876116
1104	9	1.3526969276079583
1104	10	1.0613411212600994
1104	11	0.9443401659486739
1104	12	0.9522524250341249
1104	13	0.9518504807699486
1104	14	1.0971728338366802
1104	15	1.1549289958886106
1104	16	0.6504399463512058
1104	17	0.6343280702687224
1104	18	0.9541217895582577
1104	19	1.1101136057152132
1104	20	1.3797295630013622
1104	21	1.2768378565758312
1104	22	0.8774439719818403
1104	23	1.345972869642793
1104	24	1.5847125938299271
1104	25	1.5895715047691397
1105	1	0.3770291005700912
1105	2	0.3128300862619078
1105	3	-0.036004700377976206
1105	4	0.3132606711781136
1105	5	0.4301558188225556
1105	6	0.4631280230900785
1105	7	0.5631254872162863
1105	8	0.34611029110778446
1105	9	0.513095615809604
1105	10	0.1425769410988309
1105	11	0.5309226294549063
1105	12	0.7585669158790225
1105	13	0.37734403225546487
1105	14	0.1712676353527698
1105	15	0.12021384690597614
1105	16	0.5362383736358183
1105	17	0.4266507086565241
1105	18	0.40144113021003136
1105	19	0.463223406022891
1105	20	0.6412520516541278
1105	21	0.7386792576514196
1105	22	0.30686392280805563
1105	23	0.8413892031158241
1105	24	0.9157710904022309
1105	25	0.984976686878376
1106	1	0.23853206903032387
1106	2	0.3770927603930154
1106	3	0.2769784428361497
1106	4	-1.0005588635195792
1106	5	-0.010666265174066325
1106	6	0.1184506206585354
1106	7	0.4921317713100244
1106	8	0.6688419654335078
1106	9	1.1253856784359755
1106	10	0.7582391762638849
1106	11	0.8092217035665286
1106	12	0.8492055323184289
1106	13	0.7612698238609568
1106	14	0.23082394447602184
1106	15	0.5706183853060693
1106	16	0.4336177733007034
1106	17	0.44108350412876973
1106	18	0.6171056827244996
1106	19	-0.7380467589402002
1106	20	0.40806383159948467
1106	21	0.19749620356941477
1106	22	0.756265563877669
1106	23	0.6448852680204951
1106	24	0.6959494705192029
1106	25	0.9941094038396798
1107	1	0.24022900080703025
1107	2	0.145052421851247
1107	3	0.10625969441166916
1107	4	0.13175613838289735
1107	5	0.5150262213136045
1107	6	0.3512743573643604
1107	7	0.26682163311220464
1107	8	0.029434366126587008
1107	9	0.88111928686358
1107	10	0.7871048993192744
1107	11	0.788740236879697
1107	12	0.6788358226799147
1107	13	0.6596829470298502
1107	14	0.671269524804714
1107	15	0.6730089131690562
1107	16	0.35721469124513305
1107	17	0.2813405173306265
1107	18	0.2944641399558279
1107	19	0.7070647374063128
1107	20	0.626614467431807
1107	21	0.6415935483322599
1107	22	0.8229507166726293
1107	23	1.0038027134530196
1107	24	1.0899974543031163
1107	25	0.8141626731911842
1108	1	-0.2463465513877452
1108	2	-0.21128443011659215
1108	3	-0.07304554076652892
1108	4	-0.4585758489411944
1108	5	-0.06822691159504757
1108	6	-0.05916595569735961
1108	7	-0.35261928930300357
1108	8	0.04237421665675711
1108	9	0.48248243947862335
1108	10	0.34857322057586515
1108	11	0.13017890519620323
1108	12	0.21599917184965278
1108	13	-0.29330263047261695
1108	14	-0.0684584557231318
1108	15	-0.3140788184574106
1108	16	-0.8645632460848134
1108	17	-0.9470999960199862
1108	18	-0.932708867212142
1108	19	-0.6781306885385661
1108	20	-0.18747331002347067
1108	21	-0.407932547782643
1108	22	-0.28869718564070723
1108	23	-0.5035511138470383
1108	24	-0.2987136357606843
1108	25	-0.5780628079689301
1109	1	0.35232209053590324
1109	2	0.3302081993683359
1109	3	0.30689263683956014
1109	4	0.322706763257429
1109	5	0.4132487268553078
1109	6	0.4921593703086202
1109	7	0.6002672883702367
1109	8	0.49438762050766627
1109	9	1.3588676010917098
1109	10	0.7438687118774538
1109	11	0.7003477019289264
1109	12	0.8885743850179537
1109	13	0.7880780134150385
1109	14	0.5264572260568912
1109	15	0.2964930386862932
1109	16	0.6601115072119441
1109	17	0.613624814180703
1109	18	0.6131650279276997
1109	19	0.7607361764974456
1109	20	0.9964689812015095
1109	21	0.8701671981954888
1109	22	0.6683858991685128
1109	23	1.0556015616503558
1109	24	0.9629915335127066
1109	25	1.0259083612656568
1110	1	-0.06265517549486788
1110	2	-0.03630122641839506
1110	3	-0.06434990312542865
1110	4	0.1306668300438787
1110	5	0.03954935832236117
1110	6	0.12047341683959445
1110	7	-0.018763961449220545
1110	8	3.700919529912028E-4
1110	9	-3.5186448347072137
1110	10	-1.6621521791092668
1110	11	-0.7781575757028101
1110	12	-0.25723788497031563
1110	13	-0.5083980769199918
1110	14	-0.1646828810556329
1110	15	0.1007396979194013
1110	16	-0.2817825472918045
1110	17	-0.22428660711123172
1110	18	-0.06847563092410525
1110	19	-0.23916560813358245
1110	20	0.02099971394593325
1110	21	-0.13256261132447378
1110	22	0.13161146520679523
1110	23	0.18163354519639086
1110	24	0.2622335419592261
1110	25	0.40394509381365395
1111	1	0.03581657605562327
1111	2	0.13734889257606753
1111	3	0.07563613066021802
1111	4	-0.19232631358861596
1111	5	0.18189353687121468
1111	6	0.1412738399678446
1111	7	0.24641559883868425
1111	8	0.3892272729148729
1111	9	1.037563586605259
1111	10	0.47403352846669833
1111	11	0.7516233469122682
1111	12	0.6377028000368767
1111	13	0.5024387859336059
1111	14	0.3017209595704742
1111	15	0.42061343041918065
1111	16	0.2955576426851536
1111	17	0.36038398158209617
1111	18	0.40378329241827515
1111	19	0.35884847239497475
1111	20	0.6951777077978178
1111	21	0.6589311689056316
1111	22	0.7397110825855364
1111	23	1.0175965885720117
1111	24	0.9274705906976735
1111	25	1.121173984824992
1112	1	-0.2310734584472378
1112	2	-0.19495350139673562
1112	3	-0.2587055703345378
1112	4	-0.06940791601226692
1112	5	-0.15924875526147986
1112	6	-0.1599747075539284
1112	7	-0.022249038214763317
1112	8	-0.40138660795835435
1112	9	0.24114170329344375
1112	10	0.03582557136962805
1112	11	0.1747943069425304
1112	12	-0.5138548382473296
1112	13	-0.21054132790417412
1112	14	-1.2129297005774262
1112	15	-0.4683639527383079
1112	16	-0.45076058770495564
1112	17	-0.611248699464511
1112	18	-0.5653944510090056
1112	19	-0.37327612168591884
1112	20	-0.3264412391835947
1112	21	-0.5638708181397689
1112	22	-0.3221611995236273
1112	23	-0.11549669226478443
1112	24	-0.12168717359180903
1112	25	-0.18436502522698106
1113	1	-2.179411188063927
1113	2	-2.164856783162854
1113	3	-2.176019797658032
1113	4	-2.1206305580597444
1113	5	-2.074293549824347
1113	6	-1.8953065675382454
1113	7	-2.1531038182817746
1113	8	-2.0449430823767365
1113	9	-11.134431595820168
1113	10	-4.530427607024421
1113	11	-2.940162561953173
1113	12	-12.730532198576217
1113	13	-4.85465624912753
1113	14	-3.888032478553235
1113	15	-3.163095541816517
1113	16	-2.9835544851883817
1113	17	-2.8747524184825934
1113	18	-2.8797524748202292
1113	19	-4.054925797311494
1113	20	-15.702382875551553
1113	21	-17.688012354700213
1113	22	-12.604877865002106
1113	23	-18.63394920052267
1113	24	-19.81932981516401
1113	25	-16.325960994904513
1114	1	-0.5514270555549103
1114	2	-0.3431450609986406
1114	3	-0.44531410315143205
1114	4	-0.869084969410082
1114	5	-0.6514320975511296
1114	6	-0.3202923409403091
1114	7	-0.5002493826219094
1114	8	-0.42214406138766947
1114	9	0.09142324315683936
1114	10	-0.4793140649606542
1114	11	-0.6606582552995448
1114	12	-0.7546317792368846
1114	13	-0.5294389827337938
1114	14	-0.6998712179937812
1114	15	-0.531075475407313
1114	16	-0.7418045307687393
1114	17	-0.7574893396810509
1114	18	-0.7792215326603298
1114	19	-1.081392794366728
1114	20	-1.3309250221932452
1114	21	-1.456121570773231
1114	22	-1.6258227321008185
1114	23	-1.4066578895209538
1114	24	-1.1032181239392003
1114	25	-1.3573037095051461
1115	1	-0.5924271824059453
1115	2	-0.503386410693679
1115	3	-0.5076408007198445
1115	4	-0.34215241465098245
1115	5	-0.9454031380831545
1115	6	-0.8369559922954295
1115	7	-0.988555380596452
1115	8	-0.8591473407989163
1115	9	-0.37476492476065815
1115	10	-0.7960694233772188
1115	11	-0.6084761055279806
1115	12	-0.6522249367671584
1115	13	-0.9793093243374003
1115	14	-1.0442353927654011
1115	15	-0.9821016171186372
1115	16	-0.8540121755192942
1115	17	-0.8350090483811599
1115	18	-0.9157693960521556
1115	19	-0.8756042280122003
1115	20	-1.1972493990115325
1115	21	-0.7558391712506918
1115	22	-1.0762663988028365
1115	23	-0.7898270443395248
1115	24	-0.3725719530729279
1115	25	-0.45694811069546404
1116	1	-0.7215641128109027
1116	2	-0.7078461725868301
1116	3	-0.6085137869178823
1116	4	-0.5217664799705091
1116	5	-1.3462755823903194
1116	6	-1.1714478883174095
1116	7	-2.321285047496133
1116	8	-1.4786564960452324
1116	9	-0.4639612464054075
1116	10	-1.327106223186476
1116	11	-1.1770778645057582
1116	12	-2.1922506223945852
1116	13	-1.8695168143530587
1116	14	-1.3291085090172494
1116	15	-2.530644743503842
1116	16	-2.737435191792276
1116	17	-3.5484289758761633
1116	18	-2.9402975445736104
1116	19	-2.4725750580264503
1116	20	-2.7286991196667856
1116	21	-2.032596929773277
1116	22	-1.9803007763598863
1116	23	-2.1465408013231446
1116	24	-2.658038981722701
1116	25	-2.157837353619726
1201	1	0.7713344478003279
1201	2	0.9073924748879882
1201	3	0.48274073248999017
1201	4	0.9219370468730688
1201	5	0.9482681563822197
1201	6	1.0645811723279266
1201	7	1.2145564150865802
1201	8	1.2486623297045938
1201	9	2.003859432585884
1201	10	1.660892048637436
1201	11	1.6040668247636347
1201	12	1.6168871086921186
1201	13	1.4559476329716787
1201	14	1.559076223911326
1201	15	1.6589168455085925
1201	16	1.5793360750062746
1201	17	1.4487263553196925
1201	18	1.452178067678247
1201	19	1.5010770783175502
1201	20	1.787960725252148
1201	21	1.6663388775805643
1201	22	1.7543582499493198
1201	23	2.0112553193763105
1201	24	1.9494603929504208
1201	25	1.9730583418574597
1202	1	0.8975154979837363
1202	2	0.8575378349105591
1202	3	0.8443374695850139
1202	4	0.8663603523599832
1202	5	0.9899732294470098
1202	6	0.9016449853778354
1202	7	1.0225973895117697
1202	8	1.0446606537890375
1202	9	1.675368440631992
1202	10	1.2526603960649894
1202	11	1.3872572207414393
1202	12	1.4351627112949217
1202	13	1.4060587906359956
1202	14	1.3791564284414264
1202	15	1.4580194611339437
1202	16	1.3673904738570783
1202	17	1.3561268905865944
1202	18	1.498320469196834
1202	19	1.504297230347028
1202	20	1.6626060895500174
1202	21	1.7380971353124366
1202	22	1.7406362638183808
1202	23	1.9916248758572337
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2211	8	0.356847535250985
2211	9	0.8609532773927597
2211	10	0.2212772826895204
2211	11	0.7787130487776395
2211	12	0.672227853505099
2211	13	0.7201719727595162
2211	14	0.6039258563915268
2211	15	0.543372081393187
2211	16	0.6028889021599824
2211	17	0.38343814242290364
2211	18	0.18791198741436688
2211	19	0.05318548138802015
2211	20	0.6192772809502358
2211	21	0.7157738540633716
2211	22	0.5637508751198439
2211	23	0.9878643638248263
2211	24	1.049723052531391
2211	25	1.3041030754310512
2212	1	-0.01874650577925152
2212	2	-0.016918364122382457
2212	3	0.07119855461946756
2212	4	0.19217950162594377
2212	5	-0.13131197304607412
2212	6	-0.061537115710919466
2212	7	-0.03228785685898217
2212	8	0.05176812149880661
2212	9	0.5970376522567094
2212	10	0.04706011054215509
2212	11	0.3563123325301447
2212	12	0.13371156852793575
2212	13	-0.18807869115119047
2212	14	-0.07207019894730138
2212	15	0.2688005711595949
2212	16	0.35668734208812936
2212	17	0.1720819096121815
2212	18	0.1379758704827978
2212	19	0.273150123292659
2212	20	0.5138881999389326
2212	21	0.5333533387967364
2212	22	0.19260292950468738
2212	23	0.5995361316728136
2212	24	0.6241658502122931
2212	25	0.605779177863532
2213	1	-0.4676391544295484
2213	2	-0.5055891614458403
2213	3	-0.5091636780691182
2213	4	-0.488519585361729
2213	5	-0.4679845079889873
2213	6	-0.5020568011103421
2213	7	-0.5700845096551781
2213	8	-0.5503334073014727
2213	9	0.06057774706366814
2213	10	-0.5498556149139695
2213	11	-0.20202023868939278
2213	12	-0.23335063520032406
2213	13	-0.6799598928522279
2213	14	-0.5595989687432095
2213	15	-0.5478588554259858
2213	16	-0.7977790258299251
2213	17	-0.7648619378431221
2213	18	-0.9807412633596257
2213	19	-0.8320513033843682
2213	20	-0.5505168940296983
2213	21	-0.6260820110897072
2213	22	-0.6361401924182815
2213	23	-1.1101949183675792
2213	24	-0.7095820730276046
2213	25	-0.44733024643570474
2214	1	-0.8867320995583263
2214	2	-0.8105956937340046
2214	3	-0.7986513600400045
2214	4	-0.5888835271310455
2214	5	-0.8799059338941753
2214	6	-0.6447033110020932
2214	7	-0.6274706612042635
2214	8	-0.5418087712432431
2214	9	-10.585423559393966
2214	10	-3.6620896574671598
2214	11	-1.6328059796375065
2214	12	-0.977093636320145
2214	13	-0.5841638868798498
2214	14	-0.77053121958307
2214	15	-0.6690467986883988
2214	16	-0.9092385256432891
2214	17	-0.7194532032406613
2214	18	-1.1702336112506302
2214	19	-1.2785005381734678
2214	20	-0.9593308171353385
2214	21	-1.471957337704115
2214	22	-1.252806859084945
2214	23	-1.684163750021181
2214	24	-2.0512347419339143
2214	25	-9.42448590153089
2215	1	-0.6393252251822972
2215	2	-0.49463387102600365
2215	3	-0.4214836690887154
2215	4	-0.6662072918308226
2215	5	-0.6771389067830498
2215	6	-0.6747138779890101
2215	7	-0.6952865687334047
2215	8	-0.7347997147520005
2215	9	-0.0859575812021518
2215	10	0.08926687137488187
2215	11	0.21421905699039456
2215	12	0.12646526652840606
2215	13	0.09545625263030644
2215	14	-0.15206184439669101
2215	15	-0.5678314331225138
2215	16	-0.4367499678101012
2215	17	-0.4517016376410652
2215	18	-0.5235032185217534
2215	19	-0.505600807858535
2215	20	-0.32798009041187726
2215	21	-0.18395670238833617
2215	22	-0.3847386080185231
2215	23	-0.08933071489729372
2215	24	0.02162803634087851
2215	25	0.04012765792299433
2216	1	-0.6998829135385591
2216	2	-0.7230619026057141
2216	3	-0.7158762892282908
2216	4	-0.6222603399581246
2216	5	-1.5141778177850256
2216	6	-2.047879125691228
2216	7	-1.3329016201791575
2216	8	-2.168323267558513
2216	9	-0.85101690482594
2216	10	-1.5313971520085943
2216	11	-0.9811318615938305
2216	12	-1.9821963723775617
2216	13	-1.6361208973180794
2216	14	-1.6636829236326527
2216	15	-1.4472480157991505
2216	16	-2.432443736251024
2216	17	-3.0995313245484475
2216	18	-2.3687974489145844
2216	19	-3.6604515465511476
2216	20	-2.5464491112898635
2216	21	-2.585425032125194
2216	22	-2.4210283902562786
2216	23	-2.1513125113282143
2216	24	-2.2715412802415536
2216	25	-2.364689782548311
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54373.0
3	1.0
4	775.0
5	7674.0
6	11033.0
7	8656.0
8	6660.0
9	5226.0
10	4618.0
11	4030.0
12	3594.0
13	3309.0
14	3279.0
15	3146.0
16	3276.0
17	3570.0
18	4075.0
19	5380.0
20	7498.0
21	9588.0
22	11653.0
23	13560.0
24	15374.0
25	17180.0
26	18927.0
27	20479.0
28	22815.0
29	26346.0
30	31883.0
31	38927.0
32	48355.0
33	59298.0
34	74713.0
35	93915.0
36	124141.0
37	188606.0
38	424296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52438101698453	19.564240595313674	13.483408269340591	22.427970118361205
2	15.263223864722328	22.56803841146258	40.806672593288134	21.362065130526954
3	18.144735926922007	29.877106904386537	30.02201091996661	21.956146248724846
4	11.216782243965067	18.79546984327327	38.70493046886151	31.282817443900147
5	11.233352466762913	40.01474143779026	34.83806160964325	13.913844485803576
6	26.58609045983508	39.667260843731434	20.021230960972712	13.725417735460777
7	24.7129577023862	34.19897734387682	23.69638881803214	17.391676135704845
8	25.17578354309469	35.33165872119737	21.477118063054508	18.01543967265343
9	26.112698350575535	16.38683417478768	21.277271165729545	36.22319630890724
10	14.804847465735058	31.5265023302336	32.874558777696436	20.7940914263349
11	31.58645483010553	24.930273686651976	24.60280644787197	18.88046503537052
12	22.298632370256318	28.717036500317	31.23630105968662	17.748030069740057
13	27.63973287677684	23.709033630950007	27.690450641833316	20.960782850439834
14	21.126487067700662	23.082073022550464	31.068891837040784	24.722548072708094
15	22.595835452705042	29.970959173292872	26.99666552184366	20.436539852158425
16	20.274845864740648	29.729465357697414	28.82693448898247	21.168754288579464
17	19.867369238706388	28.75842709655728	29.882281504153408	21.491922160582927
18	19.02415836303703	26.98448354696566	33.64243535777406	20.34892273222325
19	20.644597472595873	26.7605705246331	33.58263411481073	19.0121978879603
20	20.867040378890863	25.849982753973105	33.833154109916606	19.44982275721942
21	21.730907878862535	25.4938645083455	33.24534358390969	19.52988402888228
22	20.922964779261733	25.878489612316823	33.475424897978115	19.723120710443332
23	20.30365563474375	25.922241318845664	33.70818105144785	20.065921994962743
24	19.81597572427225	26.296024182983807	34.001307109982726	19.886692982761218
25	20.181954531674506	25.931204244832124	33.893818274652126	19.993022948841244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1749.0
1	1749.0
2	2674.0
3	3599.0
4	3599.0
5	3599.0
6	11924.5
7	20250.0
8	20250.0
9	20250.0
10	17395.5
11	14541.0
12	14541.0
13	14541.0
14	13423.0
15	12305.0
16	12305.0
17	12305.0
18	20235.5
19	28166.0
20	28166.0
21	28166.0
22	40819.5
23	53473.0
24	53473.0
25	53473.0
26	75200.0
27	96927.0
28	96927.0
29	96927.0
30	109985.5
31	123044.0
32	123044.0
33	123044.0
34	131477.0
35	139910.0
36	139910.0
37	139910.0
38	142728.0
39	145546.0
40	145546.0
41	145546.0
42	149953.5
43	154361.0
44	154361.0
45	154361.0
46	158723.5
47	163086.0
48	163086.0
49	163086.0
50	156055.5
51	149025.0
52	149025.0
53	149025.0
54	130874.5
55	112724.0
56	112724.0
57	112724.0
58	93999.0
59	75274.0
60	75274.0
61	75274.0
62	60455.0
63	45636.0
64	45636.0
65	45636.0
66	34144.5
67	22653.0
68	22653.0
69	22653.0
70	16658.5
71	10664.0
72	10664.0
73	10664.0
74	7412.5
75	4161.0
76	4161.0
77	4161.0
78	3055.5
79	1950.0
80	1950.0
81	1950.0
82	1327.0
83	704.0
84	704.0
85	704.0
86	492.5
87	281.0
88	281.0
89	281.0
90	237.0
91	193.0
92	193.0
93	193.0
94	100.0
95	7.0
96	7.0
97	7.0
98	3.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027893921950632828
2	0.0011592279252211047
3	3.622587266315952E-4
4	0.004057297738273866
5	0.032241026670211975
6	0.012244344960147917
7	0.021228361380611478
8	0.02948786034781185
9	0.034414579030001544
10	0.039051490730885964
11	0.039776008184149156
12	0.00753498151393718
13	0.0030429733037054
14	0.005796139626105523
15	0.007462529768610861
16	0.007969691985895094
17	0.01137492401623209
18	0.015214866518527
19	0.01709861189701129
20	0.014562800810590125
21	0.01043305132698994
22	0.009636082128400433
23	0.008187047221874051
24	0.006158398352737118
25	0.103098833599352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1380229.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.40028915823615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.94691110701417	46.46376104008652
2	11.464901860404876	13.161773639160787
3	3.3922235116192736	5.841438313689407
4	1.4208213156603284	3.2622221744435356
5	0.7065186413086251	2.0277187153399603
6	0.4102690170426182	1.4129736126546957
7	0.27963622175829483	1.1235839991629941
8	0.1916407094657781	0.8800185710260148
9	0.1537094710828672	0.7940671277864506
>10	0.9076466547488612	9.956120888080815
>50	0.08109064218038003	3.2056396181265865
>100	0.041090750162826054	4.130476670069855
>500	0.0015171846647770912	0.6350081971380482
>1k	0.0012643205539809093	1.3141403924315174
>5k	2.5286411079618185E-4	1.0115862011322576
>10k+	5.057282215923637E-4	4.779470839670581
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	20101	1.456352532804339	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	16909	1.2250865617227287	No Hit
TATCAACGCAGAGTACTTTTTTTTT	14805	1.0726480895561532	No Hit
GGTATCAACGCAGAGTACTTTTTTT	14043	1.0174398596174983	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	8158	0.5910613383721107	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5781	0.41884353973145033	No Hit
GTGGTATCAACGCAGAGTACTTTTT	3393	0.24582877189220048	No Hit
GTATAACGCAGAGTACTTTTTTTTT	2545	0.18438969185548196	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2386	0.17286986434859722	No Hit
GTGGTATCAACGCAGAGTACATGGG	2175	0.1575825460847439	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2136	0.1547569280170175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	7.245174532631904E-5	0.0	0.0	7.245174532631904E-5	0.0
7	1.4490349065263808E-4	0.0	0.0	7.245174532631904E-5	0.0
8	1.4490349065263808E-4	0.0	0.0	7.245174532631904E-5	0.0
9	1.4490349065263808E-4	0.0	0.0	7.245174532631904E-5	0.0
10	1.4490349065263808E-4	0.0	0.0	7.245174532631904E-5	0.0
11	1.4490349065263808E-4	0.0	0.0	7.245174532631904E-5	0.0
12	2.8980698130527617E-4	0.0	0.0	7.245174532631904E-5	1.4490349065263808E-4
13	2.8980698130527617E-4	0.0	0.0	7.245174532631904E-5	1.4490349065263808E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTAT	30	7.7106385E-4	19.004206	8
TAACGCA	355	0.0	17.131174	4
ATAACGC	355	0.0	16.593418	3
TATAACG	345	0.0	16.522402	2
GTTCCGT	40	0.005270959	14.253155	6
TATCACG	150	0.0	13.933891	2
GTATAAC	510	0.0	13.411816	1
GGTATCA	3540	0.0	12.961914	1
GTACCGT	60	4.0838734E-4	12.669471	6
CAACCGT	55	0.003063062	12.093586	6
GTGGTAT	1155	0.0	11.926453	1
TAAACGC	105	2.7097303E-7	11.7649355	4
GTATCAC	195	0.0	11.692352	1
CCTATAC	115	7.05877E-8	11.56652	3
AAACGCA	125	1.8177161E-8	11.405418	5
ACAACGC	75	2.071326E-4	11.401283	3
GTTTAAG	110	4.9657865E-7	11.227316	1
TGGTATC	1140	0.0	11.167114	2
GAGTTAC	95	1.3597188E-5	11.000042	1
TAAGGCC	70	0.0014880169	10.859941	4
>>END_MODULE