FastQCFastQC Report
Thu 26 May 2016
SRR1364290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118008
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84940.4010372009926308No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN54950.25944189068218815No Hit
TATCAACGCAGAGTACTTTTTTTTT53850.25424833145106157No Hit
GTACTTTTTTTTTTTTTTTTTTTTT40910.1931531892230813No Hit
GGTATCAACGCAGAGTACTTTTTTT37990.1793666501731816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1600.014.8337962
CTACGTT456.742286E-414.7839734
CGATCGT400.00525285814.26104515
GGACCGA1800.013.7221196
TATCACG707.316008E-613.5623272
ATAACGC1700.013.4024533
GGTATCA20700.013.3461171
AGGACCG1950.013.1590495
ACCGAGT1603.6379788E-1211.874918
TAACGCA1850.011.81576254
ACGGCCC1354.6729838E-911.26798715
GCCGTAC855.281582E-511.18539916
GTATAAC3350.011.0522851
GTACTAG1053.4955046E-610.8498621
TTGACCG1855.456968E-1210.79214318
GTCCTAC1502.1573214E-910.7594461
CTAGTCG1158.730058E-710.7477114
TATCTAA3100.010.7186142
CGACAGT803.7721405E-410.687426
CGAAATC803.811142E-410.675529513
Produced by FastQC (version 0.11.5)