##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364290_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2118008
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.31862485882962	30.0	26.0	31.0	7.0	34.0
2	26.235531216123828	31.0	26.0	31.0	7.0	34.0
3	25.626654384685988	30.0	25.0	31.0	7.0	34.0
4	29.429131523582537	35.0	28.0	37.0	7.0	37.0
5	29.987210624322476	35.0	30.0	37.0	7.0	37.0
6	30.162523937586638	35.0	30.0	37.0	2.0	37.0
7	29.762973038817606	35.0	30.0	37.0	2.0	37.0
8	29.32753747861198	35.0	29.0	37.0	2.0	37.0
9	30.432845862716288	37.0	29.0	39.0	2.0	39.0
10	29.715505796012103	35.0	27.0	39.0	2.0	39.0
11	30.369359794674995	35.0	29.0	39.0	2.0	39.0
12	29.892596250816805	35.0	27.0	39.0	2.0	39.0
13	29.522893209090807	35.0	27.0	39.0	2.0	39.0
14	29.81656868151584	36.0	26.0	40.0	2.0	41.0
15	30.54360465116279	36.0	27.0	40.0	2.0	41.0
16	30.868215323077155	37.0	29.0	40.0	2.0	41.0
17	31.032084864646404	37.0	30.0	40.0	2.0	41.0
18	30.86912466808435	37.0	29.0	40.0	2.0	41.0
19	30.838960476069968	37.0	29.0	40.0	2.0	41.0
20	30.73327721141752	37.0	28.0	40.0	2.0	41.0
21	30.620705870799355	37.0	28.0	40.0	2.0	41.0
22	30.711591268777077	37.0	29.0	40.0	2.0	41.0
23	30.72462568602196	37.0	30.0	40.0	2.0	41.0
24	30.58177259009409	37.0	29.0	40.0	2.0	41.0
25	30.40458770693973	37.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.704601581590321
1101	2	0.8289544900952173
1101	3	1.6993857021992262
1101	4	0.9433266452676357
1101	5	0.4734789310692058
1101	6	0.34708798588289724
1101	7	0.6785437130203675
1101	8	0.8614553148908968
1101	9	0.8115963211401791
1101	10	1.1929831726716031
1101	11	0.6941847707524182
1101	12	0.9322907787313142
1101	13	0.9635427594694725
1101	14	1.5046924626069647
1101	15	0.7848681076227884
1101	16	0.20801420437359042
1101	17	-0.41164626129816995
1101	18	-6.010720992088945
1101	19	-7.3405692178807485
1101	20	-8.226123080863612
1101	21	-10.206610616385483
1101	22	-10.361363970151945
1101	23	-11.884634051063973
1101	24	-11.508759926007748
1101	25	-11.676323997401372
1102	1	2.0901768690639564
1102	2	2.24057846097363
1102	3	3.046972794803157
1102	4	2.5105691097161618
1102	5	1.8405495917482568
1102	6	1.9316673218017932
1102	7	2.194145536521763
1102	8	2.4246238186671754
1102	9	2.6496559591615423
1102	10	-0.7653731245319086
1102	11	1.2945164710994277
1102	12	2.3761007399478906
1102	13	2.737012685304677
1102	14	3.4452058326824897
1102	15	2.891007966097309
1102	16	2.5483982963651535
1102	17	2.2632236101041556
1102	18	2.5135977464291166
1102	19	2.298704103205189
1102	20	2.6870956032215787
1102	21	2.641500392663982
1102	22	2.55961650205327
1102	23	2.533097458767962
1102	24	2.694978081748939
1102	25	2.842088773912497
1103	1	0.29013295162873476
1103	2	0.6676370907496505
1103	3	-2.226649295666757
1103	4	0.448903540985377
1103	5	-2.224486640135801
1103	6	-6.517174355844233
1103	7	-6.942042430100987
1103	8	-1.424360590643321
1103	9	-6.248241493627212
1103	10	-3.5676513414840407
1103	11	-7.3600619215224405
1103	12	-1.320945812533445
1103	13	0.7364692070438075
1103	14	1.0381680636882002
1103	15	0.8481061215650278
1103	16	0.869687273088914
1103	17	0.5828052965336674
1103	18	0.9103204078256937
1103	19	-4.999539199385939
1103	20	-0.6330515201864166
1103	21	-5.881528812430439
1103	22	-1.5384730895145857
1103	23	-0.4832398271345397
1103	24	-5.708617658259847
1103	25	-2.681829759245165
1104	1	1.793582403932227
1104	2	1.7526787010626705
1104	3	2.6328479688968507
1104	4	2.1123144900416833
1104	5	1.7660575228450313
1104	6	1.525472864245689
1104	7	1.667817325476502
1104	8	1.9727388720170609
1104	9	2.1974439494647413
1104	10	2.456767149822266
1104	11	2.00508898440048
1104	12	2.2565042002603555
1104	13	2.4578068293464455
1104	14	2.900897895175568
1104	15	1.8709948398699119
1104	16	1.1553289735677694
1104	17	1.0099110951714323
1104	18	1.0204147164121622
1104	19	1.4310459037564627
1104	20	-9.327732954554222
1104	21	-3.3743490534412715
1104	22	-0.9683594074588058
1104	23	0.25725437910152493
1104	24	0.9813538298419182
1104	25	1.3733109116373647
1105	1	1.305689239805151
1105	2	1.161221163572165
1105	3	2.0967396252742923
1105	4	1.3871811493445811
1105	5	1.1766818901351748
1105	6	1.1537753955225654
1105	7	1.2580555579389774
1105	8	1.4650704376816357
1105	9	1.4978431057665418
1105	10	2.019920661409813
1105	11	1.6687401517945908
1105	12	1.8431411129315407
1105	13	1.8901208641406129
1105	14	2.580803850385635
1105	15	1.8898059851143891
1105	16	1.320856261247929
1105	17	0.8424736024395081
1105	18	1.3773167538791533
1105	19	1.270331767765171
1105	20	1.3089990855994564
1105	21	1.256011596188845
1105	22	1.213160197114167
1105	23	1.2724512632800327
1105	24	1.2502163756421822
1105	25	1.1440986087502942
1106	1	0.9207568448060783
1106	2	1.2072303353520581
1106	3	1.8610412937953633
1106	4	1.344813773425848
1106	5	0.569214574823814
1106	6	0.47883830589842447
1106	7	0.9639657272205504
1106	8	1.020921799538275
1106	9	1.3022468966901322
1106	10	-2.2219592340937027
1106	11	-0.06054013061396901
1106	12	0.5925119168680837
1106	13	1.386078926720721
1106	14	0.6177130554506647
1106	15	1.2544635257843737
1106	16	0.8860494285469755
1106	17	-5.671000322268895
1106	18	-1.621633832192078
1106	19	-0.207261504909102
1106	20	0.49012835430683666
1106	21	0.6846553258367791
1106	22	0.7713749325509092
1106	23	0.3885063456501179
1106	24	0.810308314972783
1106	25	0.8556624505744743
1107	1	1.0399646014603832
1107	2	1.4303857898619867
1107	3	1.9340792014253765
1107	4	1.3745539848176378
1107	5	0.8818494480184675
1107	6	1.041962466928954
1107	7	1.0325826081604177
1107	8	1.135847551383005
1107	9	1.3043143680195577
1107	10	-1.8562647482792656
1107	11	0.3100023576327473
1107	12	0.6728711449198741
1107	13	1.6459580347144538
1107	14	2.049554656892976
1107	15	1.6835820934046346
1107	16	-5.218987351949348
1107	17	-1.4882078653885848
1107	18	0.2527140411284172
1107	19	1.06415838249346
1107	20	1.4878851189320415
1107	21	1.558936446216638
1107	22	1.1496505713363483
1107	23	0.889946582415508
1107	24	1.1851074718289176
1107	25	1.3478304795059834
1108	1	-0.33293677610227235
1108	2	-3.220864456732631
1108	3	-0.05197338805113816
1108	4	-0.6007144537538096
1108	5	-0.9171045532633499
1108	6	-0.5672110810829452
1108	7	-0.25717448401489307
1108	8	0.005290802090733848
1108	9	-0.4152685070805795
1108	10	-3.45198065073566
1108	11	-1.4373274293037994
1108	12	-5.93972276791699
1108	13	-1.6986941104066453
1108	14	-5.879610718540551
1108	15	-2.790768642232049
1108	16	-7.680837238653744
1108	17	-3.3392838359345802
1108	18	-1.841349178026615
1108	19	-0.650135906042312
1108	20	-0.4820994889982124
1108	21	-0.05393654419180649
1108	22	-0.17467475815876554
1108	23	-0.26573789670532477
1108	24	-0.045580198516358195
1108	25	-0.3670524020812316
1109	1	-8.649945152387225
1109	2	-6.272242433503941
1109	3	-5.560013110088235
1109	4	-5.243720639269519
1109	5	-2.5476749015971443
1109	6	-1.501700525820059
1109	7	-1.0792076285449426
1109	8	-0.634964387802242
1109	9	-5.738731092333126
1109	10	-1.7043034919097195
1109	11	-1.2242282335823909
1109	12	-6.127661912885639
1109	13	-8.102667001169188
1109	14	-8.135043586726916
1109	15	-3.881304808772107
1109	16	-2.0731751727015286
1109	17	-1.769227128101086
1109	18	-1.092976981967066
1109	19	-0.9027756270024092
1109	20	-0.6198212189668695
1109	21	-0.6025604614573083
1109	22	-0.9700282587751623
1109	23	-1.1075021697117897
1109	24	-0.7511849505433048
1109	25	-0.7217264856970154
1110	1	-2.300224089983846
1110	2	-2.1196075864507122
1110	3	-1.4141326968872363
1110	4	-2.1741944999856315
1110	5	-5.163892515730748
1110	6	-3.8496975195343097
1110	7	-8.476254566233358
1110	8	-9.41960699859283
1110	9	-10.998354141524203
1110	10	-8.97740769378101
1110	11	-11.772480046643668
1110	12	-6.4737142651541895
1110	13	-4.769176616031853
1110	14	-4.223001982415376
1110	15	-9.800538373608273
1110	16	-11.190412990999533
1110	17	-11.92878923071817
1110	18	-8.60234240941615
1110	19	-11.740829502714647
1110	20	-12.862204199117532
1110	21	-13.70271258516517
1110	22	-14.381009233823256
1110	23	-14.977300075109115
1110	24	-15.277517886539691
1110	25	-16.10341488673871
1111	1	-0.6186624443314699
1111	2	0.16999023959270687
1111	3	-2.8942493699254648
1111	4	-3.260241844779472
1111	5	-0.9317545588529512
1111	6	-0.406656401367254
1111	7	0.0501917526423874
1111	8	0.4612950011285726
1111	9	-3.8692311430591637
1111	10	-3.864475260091627
1111	11	-0.772988110194337
1111	12	-5.656444821910128
1111	13	-0.8154071948175314
1111	14	0.5583064770474628
1111	15	0.5079449436515127
1111	16	0.3533150543665897
1111	17	0.2916289960172058
1111	18	0.6122897852394829
1111	19	0.7559892700021251
1111	20	0.8603254553538626
1111	21	0.9834785982232503
1111	22	0.6435742595408982
1111	23	0.28145015798328643
1111	24	0.6531999757482048
1111	25	0.7683262144721255
1112	1	-0.6659861086557513
1112	2	-0.1572127164565984
1112	3	-3.0831713745333786
1112	4	-0.9069408295272474
1112	5	-0.7280140294709874
1112	6	-0.6247965462096552
1112	7	-0.23622585915498817
1112	8	-3.7533048983439485
1112	9	-1.2635555986765148
1112	10	-3.708675687893688
1112	11	-1.3101648550529283
1112	12	-0.03334021174724455
1112	13	0.357550895694672
1112	14	1.0354073413202194
1112	15	0.23444646639986644
1112	16	0.061231095724672
1112	17	-0.2598521617143881
1112	18	-0.17699667853929668
1112	19	0.04345455848434199
1112	20	-0.05862020915885324
1112	21	-0.07936254998449854
1112	22	-0.2262833552183352
1112	23	-0.32335054454367906
1112	24	-0.18381713893388962
1112	25	-0.1129024618739507
1113	1	-1.5053183769436131
1113	2	-0.8790656049976313
1113	3	-3.5072000258990172
1113	4	-1.6791348042504204
1113	5	-2.284925422867577
1113	6	-2.172433958237594
1113	7	-1.9302429061995525
1113	8	-9.335555340691574
1113	9	-9.950660589039938
1113	10	-4.18751880593749
1113	11	-9.889277645642625
1113	12	-8.401065480551203
1113	13	-3.7474656832605113
1113	14	-1.9346943180913776
1113	15	-8.025245226308613
1113	16	-4.1086180733693
1113	17	-3.3565243052163147
1113	18	-2.747023734231824
1113	19	-2.3153147896838213
1113	20	-8.054823378900949
1113	21	-3.720620364897787
1113	22	-3.106934776447609
1113	23	-2.835083100633863
1113	24	-8.640503394604181
1113	25	-5.815098754934542
1114	1	-4.188026134697321
1114	2	-4.237699395712063
1114	3	-0.7820178633765238
1114	4	-0.5860980172466697
1114	5	-0.5289230184378795
1114	6	-0.6379300067269256
1114	7	-0.49189302314577077
1114	8	-0.27783361489674263
1114	9	-0.5066348647247594
1114	10	-3.300740857332176
1114	11	-1.4218742370552668
1114	12	-4.709842034279031
1114	13	-1.3403406336498804
1114	14	-6.041367766482839
1114	15	-2.129849477987669
1114	16	-1.3175645555422264
1114	17	-0.9371767379674232
1114	18	-0.581958094071684
1114	19	-0.29257481873735003
1114	20	-0.08218532648900734
1114	21	0.18137793196450858
1114	22	-0.07675610111883202
1114	23	-0.3994362743654669
1114	24	-0.3090851278743223
1114	25	-0.27143176844789707
1115	1	0.7815274836617476
1115	2	0.8826927378156526
1115	3	1.6462496680863907
1115	4	0.6155423187544393
1115	5	0.3854452935234818
1115	6	-0.33539016362000496
1115	7	-2.367633108236962
1115	8	-0.12357360081405133
1115	9	0.20679724579461478
1115	10	0.8280860148227731
1115	11	-0.08795081912661118
1115	12	0.6084813320792044
1115	13	0.8144003737584065
1115	14	1.573718564381064
1115	15	0.5849264386269297
1115	16	0.3331301574981502
1115	17	-0.08495078019566193
1115	18	0.2735802713444002
1115	19	0.3379975137331712
1115	20	0.4836661308711818
1115	21	0.4732615414971022
1115	22	0.2180333031436632
1115	23	-0.0054977820068167205
1115	24	0.02468952923198131
1115	25	0.21934506056641112
1116	1	-0.08745128973503569
1116	2	0.08080552045351297
1116	3	0.9456431829249325
1116	4	0.2423683730328854
1116	5	-0.6115382586834848
1116	6	-1.4469471493965536
1116	7	-5.008727655053928
1116	8	-1.4828350995115294
1116	9	-1.5534351626004081
1116	10	-0.7464021036993671
1116	11	-2.453497590548661
1116	12	-0.9073717517152886
1116	13	-6.857552373591947
1116	14	-2.0669142555673474
1116	15	-1.761043113568462
1116	16	-1.259846913925589
1116	17	-1.0163887436052512
1116	18	-0.9313721078825701
1116	19	-0.7157379321201063
1116	20	-0.5856563082242765
1116	21	-0.4603617613421278
1116	22	-0.6560345306980757
1116	23	-0.6851433770695863
1116	24	-0.5995850752115146
1116	25	-0.6503787663768463
1201	1	2.2268952231736137
1201	2	2.3794610156170535
1201	3	3.1554763081979758
1201	4	2.7284988249284865
1201	5	2.089056821233747
1201	6	1.9817310865394724
1201	7	2.1686058588081742
1201	8	2.388470056969936
1201	9	2.7339753233722135
1201	10	3.071796650137866
1201	11	2.614982840478845
1201	12	2.8517307862301102
1201	13	2.9805116380427563
1201	14	3.596108673802725
1201	15	3.042981294701068
1201	16	2.5432760031993844
1201	17	2.3065984116311355
1201	18	2.4494508426083037
1201	19	2.441266854463592
1201	20	2.559316980877739
1201	21	2.453662566239199
1201	22	1.6199438558588817
1201	23	0.9876429133134188
1201	24	1.6360463733607027
1201	25	1.6908840004251182
1202	1	2.2401669425203785
1202	2	2.461075946249256
1202	3	3.287075203644399
1202	4	2.700104051149559
1202	5	2.1027054715709
1202	6	2.039076659370455
1202	7	2.2816976508755467
1202	8	2.6014610564409857
1202	9	2.622659896690493
1202	10	-0.9758861613924736
1202	11	1.4344724634229031
1202	12	2.4143096606017203
1202	13	2.996471854122092
1202	14	3.6088035429597696
1202	15	3.104920844133172
1202	16	2.6483668623104926
1202	17	2.298864037607302
1202	18	2.6106669344378552
1202	19	2.626346218220739
1202	20	2.6503260313864914
1202	21	2.939176754254877
1202	22	2.6201187851582866
1202	23	2.4741337899174596
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2211	17	0.01771659004802828
2211	18	0.33068074028142647
2211	19	0.6924815188698723
2211	20	0.8172762894258909
2211	21	0.918834273281881
2211	22	0.6963779765119504
2211	23	0.5826369990489333
2211	24	0.7063789862086871
2211	25	0.7402892158550713
2212	1	0.5395624579596507
2212	2	0.6615366497196327
2212	3	1.3362245128408539
2212	4	0.8550950333740523
2212	5	0.5305681329471383
2212	6	0.3292789092005819
2212	7	0.4125656222535454
2212	8	0.7996086795231356
2212	9	0.6898435824860307
2212	10	1.4517312164900673
2212	11	0.7287074624582104
2212	12	0.9012735643338168
2212	13	1.097752462129879
2212	14	-4.419877177455632
2212	15	-1.218606070780968
2212	16	-0.49001396464049307
2212	17	-0.11440056489754369
2212	18	0.05838698913171214
2212	19	0.5625919714776266
2212	20	0.6305592352553262
2212	21	0.7910419657741414
2212	22	0.6681508968277114
2212	23	0.5712970913460431
2212	24	0.4049802930845843
2212	25	0.6122832013466706
2213	1	0.7429629760104817
2213	2	0.8501257706521237
2213	3	1.6285840724976453
2213	4	1.0753054915252598
2213	5	0.5153601031592174
2213	6	0.3364045365277626
2213	7	0.48070225816094236
2213	8	-5.06098546002681
2213	9	-1.5789276656926958
2213	10	0.1751212116333427
2213	11	0.010566779278672556
2213	12	0.6348796838092916
2213	13	-8.176940754642018
2213	14	-2.2294651651519253
2213	15	-0.7303334465297127
2213	16	-0.12446110726636306
2213	17	-0.04434552199645836
2213	18	0.11084287386540126
2213	19	0.554596990095078
2213	20	0.7120372990403325
2213	21	0.8635164679596699
2213	22	0.3529323441322276
2213	23	0.28677062218625693
2213	24	0.47116290600347455
2213	25	0.623516809894511
2214	1	-0.9149708977681286
2214	2	-0.6107756568283449
2214	3	-6.773723100151127
2214	4	-2.498132406220929
2214	5	-1.478395404095675
2214	6	-0.9274732562623811
2214	7	-0.614621715286745
2214	8	-6.1059413040809325
2214	9	-1.8635719704568388
2214	10	-0.1633287286435383
2214	11	-0.5962930574128613
2214	12	-0.13087302331244643
2214	13	-6.254880404919092
2214	14	-1.5876075803157228
2214	15	-0.8655465895323182
2214	16	-0.7904541754682057
2214	17	-0.6199892589014304
2214	18	-0.62644589231369
2214	19	-0.25215221196488
2214	20	-0.0643250734361196
2214	21	-0.09337469889301886
2214	22	-0.19828414728134902
2214	23	-0.4312755506387447
2214	24	-0.18601170090090946
2214	25	-0.11575848431528613
2215	1	0.10243469879015521
2215	2	0.3111161949377639
2215	3	-2.9181208800482388
2215	4	-0.7623776609900652
2215	5	-0.3810269322743203
2215	6	-0.7548673865948707
2215	7	-0.1728572250221312
2215	8	0.11244617718265104
2215	9	0.5153829338081444
2215	10	1.1147636503844254
2215	11	0.2668109430165977
2215	12	0.07960922081331745
2215	13	0.687839632121765
2215	14	0.9483421830203689
2215	15	0.5282651714434259
2215	16	0.21460916557358445
2215	17	0.04255493471757532
2215	18	0.13325581805800013
2215	19	0.11914095518786283
2215	20	0.3738371598189545
2215	21	0.6284543276856418
2215	22	0.3460217853791079
2215	23	0.23451676388645382
2215	24	0.1945648313332775
2215	25	0.33262647219777364
2216	1	-0.1103292787605028
2216	2	-0.27352183143116804
2216	3	0.8109059309395619
2216	4	-0.7420333404624522
2216	5	-0.8628421208270503
2216	6	-1.1363566334206716
2216	7	-1.5266634862548898
2216	8	-6.413993585385803
2216	9	-2.4236003602269562
2216	10	-1.1155775434248412
2216	11	-1.8393540000845938
2216	12	-0.6666793976830903
2216	13	-0.07683168508920346
2216	14	0.4854023379297736
2216	15	0.12199605968854144
2216	16	-0.608990010697859
2216	17	-0.7633116846198078
2216	18	-0.4743817513290196
2216	19	-0.40193088717908765
2216	20	-0.23576194844428855
2216	21	-0.41487178737925134
2216	22	-0.5636309000738571
2216	23	-0.9681597733683525
2216	24	-0.9003689726388977
2216	25	-0.7629679235950064
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	197405.0
3	6.0
4	1334.0
5	8756.0
6	12588.0
7	11290.0
8	10126.0
9	8606.0
10	7502.0
11	6856.0
12	6456.0
13	6093.0
14	6134.0
15	6467.0
16	6609.0
17	7167.0
18	8129.0
19	10552.0
20	14379.0
21	18104.0
22	22414.0
23	26374.0
24	31012.0
25	34686.0
26	38882.0
27	43381.0
28	47300.0
29	55401.0
30	62587.0
31	70156.0
32	87362.0
33	109859.0
34	137914.0
35	172075.0
36	215640.0
37	256829.0
38	351577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.96675034339033	17.352484251409084	13.263060673518684	23.4177047316819
2	14.934243120939039	19.964741467425647	41.4140926138016	23.686922797833713
3	16.74826256399179	26.26502063227465	30.330367458656106	26.65634934507746
4	11.284026048404709	16.484848599687446	37.24664174632507	34.98448360558278
5	12.950037686222288	37.78073393148071	33.5594980413113	15.709730340985697
6	31.056914033555156	36.099941052392346	18.528724530017115	14.314420384035378
7	28.33920889455005	32.290663895011726	21.261260834669045	18.10886637576917
8	25.768682409572836	35.02800568021202	20.69806566904668	18.505246241168468
9	26.74513580815119	17.15567828788402	19.696976153847245	36.40220975011755
10	13.689984370800277	29.82712123603884	33.42686202929302	23.056032363867867
11	33.96576093261329	24.359597805782904	22.41354410570501	19.261097155898792
12	22.68103720132543	25.91559125987138	29.920962690715626	21.482408848087566
13	27.333904342272035	22.908016899213134	26.51195449352312	23.246124264991707
14	20.90318945434573	21.828822515577947	28.79467414155571	28.473313888520618
15	23.796798912927684	29.016355798389746	24.217660663937938	22.969184624744628
16	23.1462015882666	28.991973439243026	25.107765763820627	22.754059208669748
17	22.227830026096804	28.31195529075779	26.25465405636758	23.20556062677782
18	21.462468165373867	27.843029193399705	28.141217667643424	22.553284973583
19	23.266929441426086	27.025981558420035	28.192464756223146	21.514624243930733
20	23.457267428484144	26.549350127536197	27.801515064546077	22.191867379433585
21	23.751300272672566	26.21733220268203	28.098436737266084	21.932930787379316
22	23.488548378620106	25.967787831684625	27.813948379282966	22.729715410412304
23	22.7952201737869	26.226027128103567	27.85372281117565	23.12502988693388
24	22.568354806758116	26.461174545516524	28.343405610046645	22.62706503767871
25	22.490848352514075	26.127267080392464	28.184328288959126	23.197556278134332
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7994.0
1	7994.0
2	6206.0
3	4418.0
4	4418.0
5	4418.0
6	7587.0
7	10756.0
8	10756.0
9	10756.0
10	11426.0
11	12096.0
12	12096.0
13	12096.0
14	14262.0
15	16428.0
16	16428.0
17	16428.0
18	23855.0
19	31282.0
20	31282.0
21	31282.0
22	43376.5
23	55471.0
24	55471.0
25	55471.0
26	74080.5
27	92690.0
28	92690.0
29	92690.0
30	113486.5
31	134283.0
32	134283.0
33	134283.0
34	152500.0
35	170717.0
36	170717.0
37	170717.0
38	188221.5
39	205726.0
40	205726.0
41	205726.0
42	221959.0
43	238192.0
44	238192.0
45	238192.0
46	250253.5
47	262315.0
48	262315.0
49	262315.0
50	261573.0
51	260831.0
52	260831.0
53	260831.0
54	242211.0
55	223591.0
56	223591.0
57	223591.0
58	196444.0
59	169297.0
60	169297.0
61	169297.0
62	139337.5
63	109378.0
64	109378.0
65	109378.0
66	84906.0
67	60434.0
68	60434.0
69	60434.0
70	45392.5
71	30351.0
72	30351.0
73	30351.0
74	21365.0
75	12379.0
76	12379.0
77	12379.0
78	9138.5
79	5898.0
80	5898.0
81	5898.0
82	4062.5
83	2227.0
84	2227.0
85	2227.0
86	1532.0
87	837.0
88	837.0
89	837.0
90	581.0
91	325.0
92	325.0
93	325.0
94	200.0
95	75.0
96	75.0
97	75.0
98	46.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3167126847490661
2	0.3185068233925462
3	0.3302631529248237
4	0.3312074364213922
5	0.33791184924702833
6	0.2815381245018905
7	0.290508817719291
8	0.28871467907581083
9	0.2877703955792424
10	0.40108441516745924
11	0.28767596722958555
12	0.28772318140441394
13	0.2812548394529199
14	0.2855513293623065
15	0.2791774157604693
16	0.28422933246711063
17	0.2772416345925039
18	0.27955512915909664
19	0.2801216992570377
20	0.47115025061284
21	0.28097155440394933
22	0.2808771260542925
23	0.27766656216595975
24	0.2804521984808367
25	0.27478649750142586
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2118008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.15240169552209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.09244880807454	61.60956808013708
2	6.380076455207714	8.823952197573329
3	1.7634303732259886	3.6583633659422397
4	0.7952386143995432	2.1997064042699037
5	0.4225733876982265	1.4610982325972675
6	0.27827589773538425	1.154606799742759
7	0.19429138115324346	0.9405000944840817
8	0.15083363247640408	0.8344406353762432
9	0.10959509916412052	0.6820887889132055
>10	0.7144564893018224	9.264236389831588
>50	0.06422011863764775	3.04632931184175
>100	0.03222071292516129	3.8243190574045496
>500	0.0016510800001652089	0.7963786281349918
>1k	4.8156500004816995E-4	0.7827250638413799
>5k	2.0638500002064428E-4	0.9216869499096147
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8494	0.4010372009926308	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5495	0.25944189068218815	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5385	0.25424833145106157	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4091	0.1931531892230813	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3799	0.1793666501731816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.721417482842369E-5	0.0
4	0.0	0.0	0.0	4.721417482842369E-5	0.0
5	4.721417482842369E-5	0.0	0.0	4.721417482842369E-5	0.0
6	4.721417482842369E-5	0.0	0.0	4.721417482842369E-5	0.0
7	9.442834965684738E-5	0.0	0.0	4.721417482842369E-5	0.0
8	1.4164252448527106E-4	0.0	0.0	4.721417482842369E-5	0.0
9	1.4164252448527106E-4	0.0	0.0	9.442834965684738E-5	0.0
10	1.4164252448527106E-4	0.0	0.0	9.442834965684738E-5	0.0
11	1.4164252448527106E-4	0.0	0.0	9.442834965684738E-5	0.0
12	1.4164252448527106E-4	0.0	0.0	9.442834965684738E-5	0.0
13	1.4164252448527106E-4	0.0	0.0	9.442834965684738E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	160	0.0	14.833796	2
CTACGTT	45	6.742286E-4	14.783973	4
CGATCGT	40	0.005252858	14.261045	15
GGACCGA	180	0.0	13.722119	6
TATCACG	70	7.316008E-6	13.562327	2
ATAACGC	170	0.0	13.402453	3
GGTATCA	2070	0.0	13.346117	1
AGGACCG	195	0.0	13.159049	5
ACCGAGT	160	3.6379788E-12	11.87491	8
TAACGCA	185	0.0	11.8157625	4
ACGGCCC	135	4.6729838E-9	11.267987	15
GCCGTAC	85	5.281582E-5	11.185399	16
GTATAAC	335	0.0	11.052285	1
GTACTAG	105	3.4955046E-6	10.849862	1
TTGACCG	185	5.456968E-12	10.792143	18
GTCCTAC	150	2.1573214E-9	10.759446	1
CTAGTCG	115	8.730058E-7	10.74771	14
TATCTAA	310	0.0	10.718614	2
CGACAGT	80	3.7721405E-4	10.68742	6
CGAAATC	80	3.811142E-4	10.6755295	13
>>END_MODULE