FastQCFastQC Report
Thu 26 May 2016
SRR1364290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118008
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT174400.8234152090077091No Hit
TATCAACGCAGAGTACTTTTTTTTT134150.6333781553233038No Hit
GGTATCAACGCAGAGTACTTTTTTT127490.6019335148875736No Hit
GTACTTTTTTTTTTTTTTTTTTTTT83790.3956075708873621No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54970.259536319031845No Hit
GAGTACTTTTTTTTTTTTTTTTTTT52830.24943248561856232No Hit
GTGGTATCAACGCAGAGTACATGGG28580.1349381116596349No Hit
GTGGTATCAACGCAGAGTACTTTTT24220.11435273143444216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2350.015.3610712
ATAACGC2650.013.981553
CGTGAAC759.674204E-713.9337493
GGTATCA33300.013.6383311
ATATCTA3350.013.6136331
TACGAGA707.257964E-613.5727964
TCGCACG707.282797E-613.56830913
TGGTATC9750.013.3481712
CCGGGCG500.001498455613.3016549
TGCGTAG500.001502461513.29694314
GTGGTAT10400.013.0641241
TAACGCA2950.012.8826534
GCACCGA1750.012.48490711
ACCGACT1850.012.32149113
GTATAAC4000.011.8764761
CCGATCA657.988609E-411.69873619
TATCTAA4050.011.4933562
CGCGCCA1751.8189894E-1211.40060910
CGCGGTT1303.281093E-810.95850118
ACCGAGT2450.010.8587498
Produced by FastQC (version 0.11.5)