FastQCFastQC Report
Thu 26 May 2016
SRR1364289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233264
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85680.6947417584556105No Hit
GTACTTTTTTTTTTTTTTTTTTTTT64450.5225969459904772No Hit
TATCAACGCAGAGTACTTTTTTTTT53410.43307840008303167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43430.3521549319529314No Hit
GGTATCAACGCAGAGTACTTTTTTT36820.2985573243036365No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN34260.2777994006149535No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24230.19647050428780863No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA17510.14198095460501564No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17390.14100792693210862No Hit
GTGGTATCAACGCAGAGTACATGGG14980.12146628783455936No Hit
GAACAAAAAAAAAAAAAAAAAAAAA13330.10808715733208786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC2100.016.7260823
TATCACG1001.8189894E-1216.1384092
TAACGCA2400.015.0517034
TATAACG2400.015.030872
CGCATAT653.3645083E-614.6179868
CGAAACC602.553256E-514.25834718
GTTCTCG551.948566E-413.82740215
GGTATCA19700.013.7337911
ACGAAAC655.418728E-513.162086517
CGTGCGC604.0862043E-412.66840510
ATCACGC1302.0008883E-1012.414163
CACGCAG1501.4551915E-1112.0408725
GTATAAC3400.012.0060821
TCACGCA1353.6743586E-1011.9709464
GCGTGCG802.8611896E-511.876639
GTACAAG2950.011.5848991
TCAACTC4600.011.1591615
TCTTCGC600.005845370211.09072914
TTTGCGC951.3500328E-511.00689117
GGACAAT951.3561457E-511.0024076
Produced by FastQC (version 0.11.5)