##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364289_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1233264
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.45615942734078	31.0	26.0	31.0	10.0	34.0
2	26.351067573528457	31.0	26.0	31.0	10.0	34.0
3	25.88064518221565	30.0	25.0	31.0	10.0	34.0
4	29.60231872494454	35.0	28.0	37.0	10.0	37.0
5	30.308270573048432	35.0	30.0	37.0	10.0	37.0
6	30.378627771507155	35.0	31.0	37.0	8.0	37.0
7	29.957781950985353	35.0	30.0	37.0	2.0	37.0
8	29.513148036430156	35.0	29.0	37.0	2.0	37.0
9	30.530703077362187	37.0	29.0	39.0	2.0	39.0
10	29.88194255244619	35.0	27.0	39.0	2.0	39.0
11	30.520246273304014	37.0	30.0	39.0	2.0	39.0
12	30.072472722790902	35.0	27.0	39.0	2.0	39.0
13	29.676276936649412	35.0	27.0	39.0	2.0	39.0
14	30.025983082292193	36.0	27.0	40.0	2.0	41.0
15	30.74213226040815	36.0	27.0	40.0	2.0	41.0
16	31.11033647296929	37.0	30.0	40.0	2.0	41.0
17	31.309992832029476	37.0	30.0	40.0	2.0	41.0
18	31.161865585957266	37.0	30.0	40.0	2.0	41.0
19	31.145698731171915	38.0	30.0	40.0	2.0	41.0
20	31.03330187210524	38.0	29.0	40.0	2.0	41.0
21	30.941407516963118	38.0	29.0	40.0	2.0	41.0
22	31.052038330803462	38.0	30.0	40.0	2.0	41.0
23	31.07471879500253	38.0	30.0	40.0	2.0	41.0
24	30.942785161976673	38.0	30.0	40.0	2.0	41.0
25	30.79042038038895	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.48910090412667273
1101	2	0.7103604106909529
1101	3	1.459038950282288
1101	4	0.7540473634457783
1101	5	0.08457980785127717
1101	6	0.018938427498728316
1101	7	0.43304222729743813
1101	8	0.7750018794691513
1101	9	0.6219134861719553
1101	10	1.0084660424514738
1101	11	0.4407881807536107
1101	12	0.7365856364766046
1101	13	0.6286774388531136
1101	14	1.067425872654347
1101	15	0.4619959917242582
1101	16	-0.22947825364783014
1101	17	-0.7273416443794503
1101	18	-6.577602695892189
1101	19	-7.44992177551104
1101	20	-8.82995609482305
1101	21	-10.40363782992723
1101	22	-10.460797069295726
1101	23	-11.802863212111959
1101	24	-11.320972160173888
1101	25	-11.872103334941315
1102	1	1.7193916251894805
1102	2	1.8650238479022967
1102	3	2.8000100015232583
1102	4	1.9646585911293855
1102	5	1.409004666247231
1102	6	1.3139836524883215
1102	7	1.5157017061833322
1102	8	1.7709650603144453
1102	9	1.9588594923140832
1102	10	-1.3381117020599227
1102	11	0.8338165380064986
1102	12	1.5867809596534137
1102	13	2.025785506464228
1102	14	2.56593967843569
1102	15	2.074491909350723
1102	16	1.8549840574591698
1102	17	1.6136131474510336
1102	18	1.7270894055385
1102	19	1.3966586321796584
1102	20	1.8386723538189393
1102	21	1.9128440261125128
1102	22	1.7412603067998553
1102	23	1.8040534850411056
1102	24	1.9040241604336536
1102	25	1.9962590761049945
1103	1	0.11786962616518792
1103	2	0.6910849315827576
1103	3	-2.2911625113566387
1103	4	0.3275044451697333
1103	5	-2.2418362127487548
1103	6	-5.738983414647972
1103	7	-7.513507915809793
1103	8	-1.0891064559482686
1103	9	-6.148825021252023
1103	10	-3.1314409610034772
1103	11	-7.470728014909639
1103	12	-1.3228265586679377
1103	13	0.8290460904958366
1103	14	1.5321436874207919
1103	15	1.1747728278039524
1103	16	0.7527088324295477
1103	17	0.772350837164808
1103	18	1.1638854340806688
1103	19	-4.143752881188092
1103	20	-0.18482776144340463
1103	21	-4.538263296530911
1103	22	-0.8209197061738571
1103	23	0.06202132952807915
1103	24	-5.347990250039672
1103	25	-2.0037221602578654
1104	1	1.69443062674954
1104	2	1.6876967548185746
1104	3	2.5951218059954364
1104	4	2.1222248937814925
1104	5	1.7178970219414573
1104	6	1.5167917648128117
1104	7	1.6639076344204646
1104	8	1.998214150278045
1104	9	2.0524632364638506
1104	10	2.3680765454699753
1104	11	1.9118196581313036
1104	12	2.0834008244480025
1104	13	2.3955202958557997
1104	14	2.8004690596109327
1104	15	1.7005969535524876
1104	16	1.087433355431088
1104	17	1.0369100793283081
1104	18	1.0619828012743255
1104	19	1.28225405599661
1104	20	-9.077604297247102
1104	21	-3.40904884960252
1104	22	-0.9264464014684712
1104	23	0.1446671095860843
1104	24	0.7537381489931967
1104	25	1.199587209230316
1105	1	1.1112697096945219
1105	2	1.1109920235163138
1105	3	2.018720327761468
1105	4	1.300533262790026
1105	5	1.0477229868350655
1105	6	0.7898983180728116
1105	7	1.1184024879885932
1105	8	1.5202748408080105
1105	9	1.3907653186537487
1105	10	1.9087556992885837
1105	11	1.450889962947759
1105	12	1.7608584771251152
1105	13	1.7875763405709364
1105	14	2.4610393416972727
1105	15	1.799011721699717
1105	16	1.2043753891891953
1105	17	0.620290813278352
1105	18	1.140378154572467
1105	19	1.1078627519862465
1105	20	1.221132129821921
1105	21	1.0446523901315068
1105	22	0.9570168261864325
1105	23	1.0829476258813635
1105	24	0.8560081672992581
1105	25	0.8912470291412369
1106	1	1.3977320605446515
1106	2	1.7535594841144757
1106	3	2.292343186161858
1106	4	1.871882115457403
1106	5	1.1630594729892643
1106	6	1.0436911346264672
1106	7	1.400377851522986
1106	8	1.6758973713509633
1106	9	1.840803269275277
1106	10	-1.637575497357389
1106	11	0.30402049624381533
1106	12	0.8806608981052939
1106	13	1.8227432615516825
1106	14	0.8391962327104991
1106	15	1.5305189340020071
1106	16	1.1497191959958286
1106	17	-5.622976600499072
1106	18	-1.4777002078469863
1106	19	0.3281259698621106
1106	20	0.91958693321412
1106	21	1.1793173074766266
1106	22	1.1882169528382143
1106	23	0.8801621849973174
1106	24	1.0113497172789927
1106	25	1.162325623980177
1107	1	1.0097638743289146
1107	2	1.2872667207038901
1107	3	1.761958606317343
1107	4	1.352901668421186
1107	5	0.8189423956802955
1107	6	0.8789975215420256
1107	7	1.0243345462115236
1107	8	1.1166631799939886
1107	9	1.5061011282797345
1107	10	-1.8386872430519645
1107	11	0.2610636490325895
1107	12	0.927787348323335
1107	13	1.504381923048161
1107	14	1.960371058227878
1107	15	1.8321745845224235
1107	16	-5.359961263398063
1107	17	-1.6242040185125575
1107	18	0.12627545754717318
1107	19	0.9079242836513828
1107	20	1.2490264846860732
1107	21	1.5003619877189216
1107	22	1.094455576259687
1107	23	0.8534818469535921
1107	24	1.1713151269137505
1107	25	1.290352002612753
1108	1	-0.5654652534689397
1108	2	-3.0404554205008374
1108	3	-0.12177435529304859
1108	4	-0.7499215643372885
1108	5	-0.8786629124516843
1108	6	-0.547400384662474
1108	7	-0.24854745563067837
1108	8	-0.0216531769719559
1108	9	-0.5226214080018643
1108	10	-3.6549720207288914
1108	11	-1.5494729280348238
1108	12	-6.249140777904284
1108	13	-1.8621540026460472
1108	14	-6.004664638590352
1108	15	-2.630161327069896
1108	16	-7.8627707300219
1108	17	-3.622615317039582
1108	18	-2.1257967374350137
1108	19	-0.9157835743492555
1108	20	-0.8686610956863241
1108	21	-0.4434486798043018
1108	22	-0.5501151385832301
1108	23	-0.5977015369861398
1108	24	-0.26124004674659673
1108	25	-0.6079792173018106
1109	1	-9.258157110906307
1109	2	-6.467289261695257
1109	3	-5.67871908666466
1109	4	-5.3209271615690135
1109	5	-2.6305819172161087
1109	6	-1.814364774131672
1109	7	-1.52291617999553
1109	8	-1.054873156141042
1109	9	-5.736019095512354
1109	10	-1.898628330042115
1109	11	-1.4716814136188852
1109	12	-6.336184617506259
1109	13	-8.302297677838755
1109	14	-8.479265666363467
1109	15	-4.183951294231083
1109	16	-2.6265028849453564
1109	17	-2.0117515012812532
1109	18	-1.4739121101318844
1109	19	-1.2757147871473578
1109	20	-1.0286327652150895
1109	21	-0.8883177764571535
1109	22	-1.1922737418791627
1109	23	-1.3623563420836895
1109	24	-1.0266267851163633
1109	25	-1.034000426958059
1110	1	-2.1921741101672083
1110	2	-2.0050048320327782
1110	3	-1.3449946471475371
1110	4	-2.1168775187998605
1110	5	-5.072248583882775
1110	6	-3.696595619167393
1110	7	-8.5196518291702
1110	8	-9.21185455550049
1110	9	-10.76938630418444
1110	10	-8.459453040561947
1110	11	-11.64834438479405
1110	12	-6.2947519986346165
1110	13	-4.3582443395781
1110	14	-3.848919521992933
1110	15	-9.440717123253009
1110	16	-11.041943880264402
1110	17	-11.708394763168418
1110	18	-8.058326374729077
1110	19	-11.188270219519861
1110	20	-12.68440081153192
1110	21	-13.262005876974523
1110	22	-13.914588351388893
1110	23	-14.357946388893247
1110	24	-14.907049918893156
1110	25	-15.636180766943275
1111	1	-0.5360627169049756
1111	2	0.2539450014980602
1111	3	-2.8656678597201903
1111	4	-2.8492402522799125
1111	5	-0.8406504799383043
1111	6	-0.4591005568119968
1111	7	0.2001438934188151
1111	8	0.5548770059238066
1111	9	-3.823206630351134
1111	10	-3.574581983623851
1111	11	-0.9921991333974027
1111	12	-5.888578532125955
1111	13	-0.8697927849254299
1111	14	0.3274503216953448
1111	15	0.48718480101763006
1111	16	0.4077965407050712
1111	17	0.36647121504951485
1111	18	0.6466169128957766
1111	19	0.7634819389362058
1111	20	0.8814984040879672
1111	21	1.1294618731524473
1111	22	0.726035766888856
1111	23	0.4544597958593073
1111	24	0.5534799609104688
1111	25	0.8066836312283847
1112	1	-0.7563525224891237
1112	2	-0.21051223927124596
1112	3	-3.222900398083894
1112	4	-1.0370394998750356
1112	5	-1.0287881389923257
1112	6	-0.9388599584964616
1112	7	-0.4799497405468891
1112	8	-3.7237383203947907
1112	9	-1.2587518614125166
1112	10	-3.7861439334344773
1112	11	-1.523605526061214
1112	12	-0.1563504213971143
1112	13	0.19195889700245772
1112	14	0.8481776413109507
1112	15	0.07479144968155538
1112	16	0.07012994377630832
1112	17	-0.18485074293598913
1112	18	-0.11664108226606373
1112	19	-0.06374346679441345
1112	20	0.0025798835270514076
1112	21	-0.04426195506602326
1112	22	-0.144714499979667
1112	23	-0.26563060688855344
1112	24	-0.09649403390174527
1112	25	-0.04068744942286173
1113	1	-1.6110935679024543
1113	2	-1.2397004726494707
1113	3	-3.550590075111387
1113	4	-2.0902361046584446
1113	5	-2.111941566338917
1113	6	-2.006357713797911
1113	7	-1.792412328904387
1113	8	-9.139918085537705
1113	9	-9.851257649213679
1113	10	-4.376591530726753
1113	11	-9.503747096117813
1113	12	-7.849583205439508
1113	13	-3.3163916016727306
1113	14	-1.7353097399621014
1113	15	-7.658865714427915
1113	16	-3.6293109722367554
1113	17	-3.155258133716
1113	18	-2.4318710318263648
1113	19	-2.053501479487963
1113	20	-6.6053604074915135
1113	21	-3.1926876951563408
1113	22	-2.760149223265554
1113	23	-2.7432666972398714
1113	24	-7.732847367059026
1113	25	-4.975993366624298
1114	1	-4.372569433963548
1114	2	-4.277837216794097
1114	3	-1.0818684614325207
1114	4	-0.9658494718327262
1114	5	-1.0977138969115394
1114	6	-1.2087655417434071
1114	7	-0.9810834037997473
1114	8	-0.609595928622575
1114	9	-1.1042142793677456
1114	10	-3.924468679181647
1114	11	-1.7913762260492412
1114	12	-4.895233975766338
1114	13	-1.7362041406343494
1114	14	-5.925535939979913
1114	15	-2.50327416575929
1114	16	-1.6416952005740981
1114	17	-1.5166037566566004
1114	18	-1.1603875960796373
1114	19	-1.067919504245289
1114	20	-0.8301066003849158
1114	21	-0.716228304293395
1114	22	-0.6586174751134486
1114	23	-1.2183858084697228
1114	24	-1.030961624784556
1114	25	-0.9646533096290177
1115	1	0.5012387521436743
1115	2	0.47187781756615976
1115	3	1.4211767331611078
1115	4	0.4064309647792861
1115	5	0.02233011758331216
1115	6	-0.46248481562691524
1115	7	-1.9606959094119638
1115	8	-0.29054015484824447
1115	9	-0.08299728103852999
1115	10	0.5686374340244171
1115	11	-0.165658414408707
1115	12	0.4791728467257066
1115	13	0.7536789044621095
1115	14	1.2320933617990697
1115	15	0.2211546149116259
1115	16	0.18117835221017486
1115	17	-0.2975062746095567
1115	18	0.10921320668193957
1115	19	0.2100337481412815
1115	20	0.21103640256165335
1115	21	0.30927581378517743
1115	22	-0.08193362627404355
1115	23	-0.10889644039912127
1115	24	-0.09320557892508319
1115	25	0.042847309861503646
1116	1	0.41136908120333615
1116	2	0.5881738020284928
1116	3	1.3258392985166694
1116	4	0.5925758901685825
1116	5	-0.31183797929176293
1116	6	-1.026084984204772
1116	7	-4.321026389516636
1116	8	-1.0125751464732637
1116	9	-1.0891675306097035
1116	10	-0.47261374557522373
1116	11	-2.0209261677056105
1116	12	-0.35334275586746955
1116	13	-6.276788167772754
1116	14	-1.4240593783646034
1116	15	-1.0700350356456063
1116	16	-0.5851141866257841
1116	17	-0.45562653775228057
1116	18	-0.1682696483968904
1116	19	-0.007642408921149979
1116	20	0.17967838979267725
1116	21	0.1314514901006909
1116	22	-0.15578793206471175
1116	23	-0.2594311687970361
1116	24	-0.17956703966439846
1116	25	-0.06947589083005923
1201	1	2.4133674311800952
1201	2	2.6733911879320793
1201	3	3.423522434016288
1201	4	3.0672034186428725
1201	5	2.3660829707241255
1201	6	2.3278801955432122
1201	7	2.5318881167133966
1201	8	2.5042418406919573
1201	9	2.9967408154645554
1201	10	3.208392975629856
1201	11	2.5830372005363067
1201	12	3.2280523522380413
1201	13	3.20431885911103
1201	14	3.73213488547556
1201	15	3.12560296126345
1201	16	2.8909232111405743
1201	17	2.7562689497343555
1201	18	2.6113414885353734
1201	19	2.617153279073328
1201	20	2.792851972444449
1201	21	2.637955314448785
1201	22	1.8060006765640644
1201	23	1.3589714555059196
1201	24	1.7270353306136492
1201	25	1.9265921058616122
1202	1	2.0811952831395537
1202	2	2.272933212293573
1202	3	3.0385622158456513
1202	4	2.410764243288668
1202	5	1.872810567126269
1202	6	1.5970169153637208
1202	7	2.0268360137685804
1202	8	2.2180393733495976
1202	9	2.3250233455488
1202	10	-1.048136324339179
1202	11	1.137800993835672
1202	12	2.2444563689662367
1202	13	2.4381454455382006
1202	14	3.0405482243641657
1202	15	2.5700766179165164
1202	16	2.1413209180317097
1202	17	1.8491736137110237
1202	18	2.085730055966188
1202	19	2.3233069298443425
1202	20	2.2070091123414706
1202	21	2.4110440376202753
1202	22	2.0306521538724915
1202	23	1.7622935891755382
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2211	15	-1.4337116477543823
2211	16	-0.4314425855165318
2211	17	0.009168343435781878
2211	18	0.44704929982468045
2211	19	0.7059656815653526
2211	20	0.9374478116865674
2211	21	1.0371312872311336
2211	22	0.7967224078670725
2211	23	0.6903022422320966
2211	24	0.8850629910279721
2211	25	0.8565696333413726
2212	1	0.9009999436949876
2212	2	0.8635703783433115
2212	3	1.6515528187264295
2212	4	0.9274249495143216
2212	5	0.6433931925935532
2212	6	0.33631104631613695
2212	7	0.7987666158953886
2212	8	0.9433911667291248
2212	9	0.9733743920901645
2212	10	1.7152456298907488
2212	11	0.9427399579008338
2212	12	1.1915103318444054
2212	13	1.3491288062284283
2212	14	-4.62942368514717
2212	15	-0.9322066894863497
2212	16	-0.42670732732652894
2212	17	0.22366226391056898
2212	18	0.5446595607605182
2212	19	0.9094714726581401
2212	20	1.0957742923620764
2212	21	1.1134677465225273
2212	22	1.0089294030219769
2212	23	0.7475455731872955
2212	24	0.8916966980818657
2212	25	1.0815973066181073
2213	1	0.4422905263013881
2213	2	0.5450036646110483
2213	3	1.273575267736085
2213	4	0.6834423548500475
2213	5	0.21695643013668686
2213	6	-0.09823022349868538
2213	7	0.17049306359715644
2213	8	-5.045126663018021
2213	9	-1.815499339535478
2213	10	0.1798004543075784
2213	11	-0.17018100932736147
2213	12	0.4123696882990444
2213	13	-8.793186787311246
2213	14	-2.6744856733631437
2213	15	-0.9767521394216203
2213	16	-0.48974624832901625
2213	17	-0.5321928629190182
2213	18	-0.06814899479415004
2213	19	0.20100601636696425
2213	20	0.47931302150403354
2213	21	0.520109187574981
2213	22	0.19616040285318093
2213	23	0.10992555169022111
2213	24	0.2268119556663919
2213	25	0.20017387297395572
2214	1	-0.8119240880804526
2214	2	-0.5742262002337384
2214	3	-6.807578208710691
2214	4	-2.4037079219567943
2214	5	-1.4251640357778186
2214	6	-1.136827382868546
2214	7	-0.5950854658283298
2214	8	-5.948057694764856
2214	9	-1.8329593524513648
2214	10	-0.25904958913808684
2214	11	-0.52413288201112
2214	12	-0.3112699478056271
2214	13	-5.816295698649867
2214	14	-1.2906649647587862
2214	15	-0.8369991858616004
2214	16	-0.903858949322327
2214	17	-0.8519616271434458
2214	18	-0.6653853021961673
2214	19	-0.3738431499907513
2214	20	-0.07219714218539153
2214	21	-0.041023458032920956
2214	22	-0.11082182313256794
2214	23	-0.22485012008269223
2214	24	-0.19440304196056246
2214	25	-0.1187322290865751
2215	1	0.35892450745619797
2215	2	0.44868352879128537
2215	3	-2.4133019060156364
2215	4	-0.6810702725769886
2215	5	-0.23455036989512834
2215	6	-0.6897067505108758
2215	7	0.08522265427213327
2215	8	0.236235508889024
2215	9	0.6998870502917178
2215	10	1.309368879186362
2215	11	0.4586729594040655
2215	12	0.3291909865502305
2215	13	0.9032415761641985
2215	14	0.9765628935495698
2215	15	0.41221404117039384
2215	16	0.2440687245013322
2215	17	0.28113549181000863
2215	18	0.27237118676467986
2215	19	0.3643314101974404
2215	20	0.598507894962168
2215	21	0.6040386443529222
2215	22	0.3662596883474798
2215	23	0.17896478594982668
2215	24	-0.09489152979435644
2215	25	0.12819306653421236
2216	1	0.2954703759308863
2216	2	0.015514797249736034
2216	3	1.1975910031700714
2216	4	-0.2545011780730029
2216	5	-0.5508021608899618
2216	6	-0.7133664199588132
2216	7	-1.3077909523608717
2216	8	-6.536466655885786
2216	9	-2.318303309287014
2216	10	-1.1892354002412162
2216	11	-1.5453921823573111
2216	12	-0.4206085018107082
2216	13	0.17518792117374815
2216	14	0.8339243809540626
2216	15	0.6161973826305704
2216	16	-0.1401371531999409
2216	17	-0.16891751647467856
2216	18	0.01971173836058071
2216	19	0.11679069894311667
2216	20	0.15084858969686366
2216	21	0.10387978375650064
2216	22	-0.2976457292916308
2216	23	-0.4197031315778261
2216	24	-0.2800427336385489
2216	25	-0.2102201296424333
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107536.0
3	1.0
4	582.0
5	5169.0
6	8407.0
7	7295.0
8	6276.0
9	4847.0
10	4527.0
11	4387.0
12	4082.0
13	3834.0
14	3848.0
15	3849.0
16	3762.0
17	3956.0
18	4661.0
19	6138.0
20	8665.0
21	11082.0
22	13578.0
23	15442.0
24	17877.0
25	19962.0
26	21465.0
27	23854.0
28	25876.0
29	29647.0
30	34143.0
31	38300.0
32	48156.0
33	61571.0
34	78414.0
35	99740.0
36	128811.0
37	157257.0
38	216267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.0490265396003	18.488709746971082	12.915955084702324	21.546308628726297
2	13.742166663140884	22.62933243941216	42.850773203844945	20.777727693602007
3	15.258487968258205	31.536817813422562	30.61543669380169	22.589257524517542
4	9.877442344357636	18.57333156480111	39.38103542136772	32.16819066947353
5	11.083849273777204	41.22478076600066	33.45711021269116	14.234259747530972
6	26.18818150099183	39.66891871559201	20.4071092849643	13.73579049845187
7	25.883575883575883	35.52661545619292	22.299412088144482	16.290396572086713
8	25.085403069955685	35.74419421796816	21.563543137497316	17.60685957457884
9	27.64015242402183	18.121866014892657	20.29500636455694	33.94297519652857
10	12.729549792517606	33.3287732806973	33.07023458213632	20.871442344648777
11	31.508827010122285	26.822317024071445	23.63637842476199	18.032477541044276
12	21.6703444360487	30.08664786706999	30.115847702119385	18.127159994761925
13	26.04133286703049	25.817995494213235	27.243499548608003	20.89717209014827
14	19.864787486010254	24.834156450899247	30.628432367715575	24.672623695374927
15	22.353669317748366	31.490100938465034	25.550501357801686	20.605728385984918
16	20.525060248215325	31.944933578528456	26.581276733493453	20.948729439762765
17	20.103188364308277	30.942846268205166	27.3531306217652	21.600834745721357
18	19.37852918954396	29.649949941727456	30.57027392569905	20.401246943029538
19	21.001830722797987	29.118333334688824	30.641550006547007	19.23828593596618
20	21.87127450221442	28.436951233921537	30.111678353304516	19.580095910559532
21	22.882160282316274	27.991131664366353	29.661101726413797	19.465606326903572
22	21.782339272512257	28.550433136860836	29.576200123788244	20.091027466838657
23	21.166788525679447	28.679945639223043	29.745690135438362	20.407575699659148
24	20.37241872910787	28.635738859564224	30.49278992785861	20.499052483469292
25	20.78536458418048	28.19456417429775	30.362225728273778	20.657845513247995
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8429.0
1	8429.0
2	7810.5
3	7192.0
4	7192.0
5	7192.0
6	11540.5
7	15889.0
8	15889.0
9	15889.0
10	15004.5
11	14120.0
12	14120.0
13	14120.0
14	15186.5
15	16253.0
16	16253.0
17	16253.0
18	22608.0
19	28963.0
20	28963.0
21	28963.0
22	38507.0
23	48051.0
24	48051.0
25	48051.0
26	63299.5
27	78548.0
28	78548.0
29	78548.0
30	90576.5
31	102605.0
32	102605.0
33	102605.0
34	108961.5
35	115318.0
36	115318.0
37	115318.0
38	121870.0
39	128422.0
40	128422.0
41	128422.0
42	135164.5
43	141907.0
44	141907.0
45	141907.0
46	144902.0
47	147897.0
48	147897.0
49	147897.0
50	140187.0
51	132477.0
52	132477.0
53	132477.0
54	115744.0
55	99011.0
56	99011.0
57	99011.0
58	83765.5
59	68520.0
60	68520.0
61	68520.0
62	54772.0
63	41024.0
64	41024.0
65	41024.0
66	30863.5
67	20703.0
68	20703.0
69	20703.0
70	15304.5
71	9906.0
72	9906.0
73	9906.0
74	7027.5
75	4149.0
76	4149.0
77	4149.0
78	3224.0
79	2299.0
80	2299.0
81	2299.0
82	1599.0
83	899.0
84	899.0
85	899.0
86	669.0
87	439.0
88	439.0
89	439.0
90	319.5
91	200.0
92	200.0
93	200.0
94	117.0
95	34.0
96	34.0
97	34.0
98	21.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34015425732041155
2	0.34234356958445233
3	0.352560360149976
4	0.354587501135199
5	0.3600202389755965
6	0.30139532168294864
7	0.3103958276573386
8	0.3081254297538889
9	0.30804434411447995
10	0.42229401004164563
11	0.30804434411447995
12	0.3085308579509335
13	0.30277377755290025
14	0.3074767446386175
15	0.3002601227312238
16	0.3054496036533946
17	0.29742212535191165
18	0.2997736088947703
19	0.2996114376159525
20	0.4929196019668133
21	0.3017196642405844
22	0.3036657195863984
23	0.300097951452406
24	0.30277377755290025
25	0.29547607000609766
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1233264.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.61379622540544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.2608134116379	59.0003485647761
2	7.701308516593433	10.414294094198555
3	2.1967231735682415	4.45586379063807
4	0.8730198579492899	2.361127471044627
5	0.47387981820619357	1.602040673176287
6	0.30166129833914324	1.2237879332996437
7	0.20147742526011145	0.9535857502889569
8	0.14401936766730752	0.7790156942375259
9	0.09943946794891716	0.6051131930394736
>10	0.6581018835680371	8.30233712515113
>50	0.05896126087226498	2.73971161860449
>100	0.02766915721345479	3.418479080300809
>500	0.0013407905389336637	0.6310477449210151
>1k	0.0012189004899396943	1.8357827501783484
>5k	3.6567014698190827E-4	1.6774645161450101
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8568	0.6947417584556105	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6445	0.5225969459904772	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5341	0.43307840008303167	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4343	0.3521549319529314	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3682	0.2985573243036365	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3426	0.2777994006149535	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2423	0.19647050428780863	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1751	0.14198095460501564	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1739	0.14100792693210862	No Hit
GTGGTATCAACGCAGAGTACATGGG	1498	0.12146628783455936	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	1333	0.10808715733208786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	8.108563940891812E-5	0.0	0.0	0.0	0.0
3	8.108563940891812E-5	0.0	0.0	0.0	0.0
4	8.108563940891812E-5	0.0	0.0	0.0	0.0
5	8.108563940891812E-5	0.0	0.0	8.108563940891812E-5	0.0
6	8.108563940891812E-5	0.0	0.0	8.108563940891812E-5	0.0
7	8.108563940891812E-5	0.0	0.0	8.108563940891812E-5	0.0
8	8.108563940891812E-5	0.0	0.0	8.108563940891812E-5	0.0
9	8.108563940891812E-5	0.0	0.0	8.108563940891812E-5	0.0
10	8.108563940891812E-5	0.0	0.0	8.108563940891812E-5	0.0
11	8.108563940891812E-5	0.0	0.0	8.108563940891812E-5	0.0
12	8.108563940891812E-5	0.0	0.0	8.108563940891812E-5	0.0
13	8.108563940891812E-5	0.0	0.0	8.108563940891812E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	210	0.0	16.726082	3
TATCACG	100	1.8189894E-12	16.138409	2
TAACGCA	240	0.0	15.051703	4
TATAACG	240	0.0	15.03087	2
CGCATAT	65	3.3645083E-6	14.617986	8
CGAAACC	60	2.553256E-5	14.258347	18
GTTCTCG	55	1.948566E-4	13.827402	15
GGTATCA	1970	0.0	13.733791	1
ACGAAAC	65	5.418728E-5	13.1620865	17
CGTGCGC	60	4.0862043E-4	12.668405	10
ATCACGC	130	2.0008883E-10	12.41416	3
CACGCAG	150	1.4551915E-11	12.040872	5
GTATAAC	340	0.0	12.006082	1
TCACGCA	135	3.6743586E-10	11.970946	4
GCGTGCG	80	2.8611896E-5	11.87663	9
GTACAAG	295	0.0	11.584899	1
TCAACTC	460	0.0	11.159161	5
TCTTCGC	60	0.0058453702	11.090729	14
TTTGCGC	95	1.3500328E-5	11.006891	17
GGACAAT	95	1.3561457E-5	11.002407	6
>>END_MODULE