FastQCFastQC Report
Thu 26 May 2016
SRR1364289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233264
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT181701.4733260680600424No Hit
GTACTTTTTTTTTTTTTTTTTTTTT171841.393375627602849No Hit
TATCAACGCAGAGTACTTTTTTTTT135081.095304817135666No Hit
GGTATCAACGCAGAGTACTTTTTTT121440.9847040049819017No Hit
GAGTACTTTTTTTTTTTTTTTTTTT76550.6207105696752683No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52270.42383463719041503No Hit
GTGGTATCAACGCAGAGTACTTTTT26270.2130119747272279No Hit
GTATAACGCAGAGTACTTTTTTTTT23370.18949713929864165No Hit
GTGGTATCAACGCAGAGTACATGGG21770.17652343699321474No Hit
ATCAACGCAGAGTACTTTTTTTTTT20800.1686581299705497No Hit
GCAGAGTACTTTTTTTTTTTTTTTT19450.15771156865034575No Hit
GGTATCAACGCAGAGTACATGGGAT14230.11538486487889048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAA555.924394E-717.26793514
ACGGCCC402.7663866E-416.62038615
TATAACG3800.016.4994372
TATCACG2050.016.2189582
ATAACGC3850.016.0384123
TAACGCA3950.015.8748074
TTTTCCG508.6810396E-515.2062665
GCGCCAA456.760596E-414.77787311
ACAACGC1101.8553692E-1013.817713
TCACGCA2450.013.5726184
GGTATCA31200.013.3387881
GGGCGTA500.001497336113.3022449
GGCGTAG500.00149779413.30170310
GTAAGAC500.001499626613.2995463
CGCCAAC500.001503297613.29523112
GTGTTAT1153.765308E-1013.2196211
TACAACG1153.765308E-1013.2169392
TGGTCGG655.444156E-513.15553210
GTATAAC5150.013.0992971
ATCACGC2550.013.0387713
Produced by FastQC (version 0.11.5)