##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364289_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1233264
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.71117619585101	31.0	30.0	34.0	26.0	34.0
2	29.699975836479457	31.0	30.0	34.0	26.0	34.0
3	29.75990298914101	31.0	30.0	34.0	26.0	34.0
4	33.07773031565018	35.0	33.0	37.0	27.0	37.0
5	32.694491203829834	35.0	33.0	37.0	26.0	37.0
6	32.542526985300796	35.0	33.0	37.0	25.0	37.0
7	32.266212262743416	35.0	32.0	37.0	22.0	37.0
8	32.13729744807276	35.0	32.0	37.0	19.0	37.0
9	32.86276255530041	37.0	32.0	39.0	16.0	39.0
10	33.016368758027475	37.0	32.0	39.0	17.0	39.0
11	32.92119043448929	37.0	32.0	39.0	17.0	39.0
12	32.85909748439912	37.0	32.0	39.0	17.0	39.0
13	32.90842431142075	37.0	32.0	39.0	17.0	39.0
14	33.823024915995276	38.0	33.0	41.0	17.0	41.0
15	33.86315338808236	38.0	33.0	41.0	17.0	41.0
16	33.85012373668574	38.0	33.0	41.0	17.0	41.0
17	33.881865521088756	38.0	33.0	41.0	17.0	41.0
18	33.86323933886013	38.0	33.0	41.0	17.0	41.0
19	33.80519904902762	39.0	33.0	41.0	16.0	41.0
20	33.680679886869314	39.0	33.0	41.0	11.0	41.0
21	33.618993986688984	39.0	33.0	41.0	10.0	41.0
22	33.544395198432774	39.0	33.0	41.0	10.0	41.0
23	33.278667827813024	38.0	32.0	41.0	2.0	41.0
24	33.186822124054544	38.0	32.0	41.0	2.0	41.0
25	33.05210806445335	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.23174586851637713
1101	2	0.3309584636435936
1101	3	-1.2518832244169786
1101	4	-0.31036432183172025
1101	5	0.30176867361984705
1101	6	0.5773863429647506
1101	7	0.737290505064685
1101	8	0.7565030504413244
1101	9	1.5138058441984512
1101	10	1.095327839419788
1101	11	1.1906996388515907
1101	12	1.2472802989415896
1101	13	0.9883159112756275
1101	14	1.1033309333074044
1101	15	1.054410857822873
1101	16	0.9917793954710064
1101	17	0.9798416060945669
1101	18	0.9535913882904907
1101	19	1.056602940298994
1101	20	1.1653086624434792
1101	21	1.0971487913069353
1101	22	1.275702447646104
1101	23	1.4294960868796593
1101	24	1.6364714515301557
1101	25	1.7590604363185989
1102	1	0.4084584680176597
1102	2	0.408106843379322
1102	3	0.447905823748723
1102	4	0.5484510119137056
1102	5	0.5377308452603486
1102	6	0.6867241504393107
1102	7	0.7868489078859753
1102	8	0.6961854032340895
1102	9	1.3698545551495798
1102	10	0.9985254767592409
1102	11	1.0545419337832769
1102	12	1.0071024546310525
1102	13	0.8300765250964091
1102	14	0.9182708085304441
1102	15	1.0866485031238327
1102	16	0.9385186127485952
1102	17	0.9507221232559573
1102	18	0.9293183311148354
1102	19	0.9611358116408013
1102	20	1.3665188671859028
1102	21	1.3951408942794643
1102	22	1.254529050845008
1102	23	1.5950552914580882
1102	24	1.5806100041342575
1102	25	1.52703164034196
1103	1	-2.5597371670242346
1103	2	-2.509920186980242
1103	3	-2.5593396207082293
1103	4	-2.5046715253589156
1103	5	-5.005347785101357
1103	6	-8.676572030892476
1103	7	-10.12669093267672
1103	8	-10.735630219906302
1103	9	-11.30811318211661
1103	10	-9.664219589744448
1103	11	-12.221262088068315
1103	12	-10.663970986244724
1103	13	-11.287389204460613
1103	14	-12.156035875077105
1103	15	-11.618574757352977
1103	16	-6.535123838104081
1103	17	-4.484331567551472
1103	18	-3.9121369765820084
1103	19	-3.4368141269752996
1103	20	-8.122753693407244
1103	21	-4.892173278699822
1103	22	-10.294819314842197
1103	23	-11.701150165007594
1103	24	-13.079350002513433
1103	25	-13.49900960749352
1104	1	0.18276944045655696
1104	2	0.44502832023839645
1104	3	0.46038632296869153
1104	4	0.5676917815444895
1104	5	0.6724162326758503
1104	6	0.6383622159619335
1104	7	0.585600857963982
1104	8	0.9827168644925379
1104	9	1.3168124334647118
1104	10	0.9922852271492602
1104	11	0.9432574824052224
1104	12	0.9592605409545101
1104	13	0.8888388755904231
1104	14	0.9780732006261275
1104	15	0.8604394547619023
1104	16	0.6343064442618527
1104	17	0.7785952381805501
1104	18	0.7946329436993409
1104	19	0.9626958740432983
1104	20	1.1985521485171517
1104	21	1.096648954601882
1104	22	0.8260319109594079
1104	23	1.1597978811616727
1104	24	1.3388003317473576
1104	25	1.4999905586986912
1105	1	0.19342265976230166
1105	2	0.3471732120042148
1105	3	-0.047548429389419056
1105	4	0.3005219476295977
1105	5	0.3037821909389109
1105	6	0.459624746633736
1105	7	0.6520992108618486
1105	8	0.16175509211972638
1105	9	0.6080992022062546
1105	10	0.02293374520085223
1105	11	0.7432562627402319
1105	12	0.8840144181031988
1105	13	0.5546634190199242
1105	14	0.4668603214073386
1105	15	0.4481369711913388
1105	16	0.6196105899031608
1105	17	0.638330160626488
1105	18	0.7173078464310549
1105	19	0.6094512325305601
1105	20	0.9476637334496374
1105	21	0.7548749851356362
1105	22	0.5524172563519016
1105	23	1.063908928332772
1105	24	1.042318898929281
1105	25	1.1397278539474343
1106	1	-0.050817659741511534
1106	2	0.24716574538784997
1106	3	0.04392688418039725
1106	4	-1.1078267399862582
1106	5	-0.040135492141956775
1106	6	0.3226027925360242
1106	7	0.44854128395384407
1106	8	0.4737257623524016
1106	9	0.9122198200603364
1106	10	0.4584087062116424
1106	11	0.5073633957728774
1106	12	0.789810982561427
1106	13	0.5493778405929461
1106	14	-0.03914581356887936
1106	15	0.4786875022193442
1106	16	0.18613477331918915
1106	17	0.2995156010614153
1106	18	0.24685880524565107
1106	19	-0.81876230371509
1106	20	0.2996579936988297
1106	21	-0.18314030963851735
1106	22	0.5690184076704625
1106	23	0.4687140608736371
1106	24	0.4114726225252454
1106	25	0.7618140490158751
1107	1	0.2326496597668104
1107	2	0.05914979841676171
1107	3	-0.09333880700902242
1107	4	0.0430243255016407
1107	5	0.09782128270448709
1107	6	0.1736546424581462
1107	7	0.1630858812549718
1107	8	0.15780061202848827
1107	9	0.8580394736668282
1107	10	0.7756102599391497
1107	11	0.6007484754910379
1107	12	0.5249906754453022
1107	13	0.43791102875139387
1107	14	0.5403162823155725
1107	15	0.5148537497269317
1107	16	0.2822006898414031
1107	17	0.04412247660624047
1107	18	0.23918201530279504
1107	19	0.36912950982424775
1107	20	0.091082099186643
1107	21	0.1608025355442706
1107	22	0.38632683861275297
1107	23	0.5370130543351976
1107	24	0.7317519060471156
1107	25	0.3292780642369735
1108	1	-0.06925510320385442
1108	2	-0.08477569881490155
1108	3	0.1109262884529656
1108	4	-0.19085614639697468
1108	5	0.1651236911065297
1108	6	0.03325098133785076
1108	7	-0.2705705831763545
1108	8	0.015637291950184817
1108	9	0.4505929461463154
1108	10	0.32450969664682816
1108	11	0.34135308860109603
1108	12	0.40189597003498534
1108	13	-0.20128026120257658
1108	14	0.004900382765825384
1108	15	-0.38365346406337864
1108	16	-0.6779983930878046
1108	17	-0.737468284190939
1108	18	-0.7432527326594212
1108	19	-0.6058066119534402
1108	20	0.053325397149585285
1108	21	-0.07700716051514434
1108	22	-0.17313726703057597
1108	23	-0.5733174783918287
1108	24	-0.05183494427247126
1108	25	-0.251842521506461
1109	1	0.16992191436039406
1109	2	0.15578260406828548
1109	3	0.12920283172660163
1109	4	0.18882650498220954
1109	5	0.1262421905922153
1109	6	0.3103265945370737
1109	7	0.45482989541815755
1109	8	0.3303166831194275
1109	9	1.1543899609560597
1109	10	0.5167690113786776
1109	11	0.47910879450648736
1109	12	0.5417535967742992
1109	13	0.587107903912532
1109	14	0.08646265815564647
1109	15	-0.16463648730017155
1109	16	0.32962974499876196
1109	17	0.36107640328081203
1109	18	0.17791205535161225
1109	19	0.4500457338913506
1109	20	0.6604423924195899
1109	21	0.6770476583549083
1109	22	0.10074161885992083
1109	23	0.5298132915341895
1109	24	0.3106581694755022
1109	25	0.32843046458761194
1110	1	-0.17050984222990095
1110	2	-0.046570192186948134
1110	3	-0.06781613846309753
1110	4	0.14966045423079777
1110	5	0.07983913565256984
1110	6	0.05439225110273327
1110	7	0.05493460101067171
1110	8	-0.07467530998231808
1110	9	-3.519291980408809
1110	10	-1.4839269728538937
1110	11	-0.6014713976590684
1110	12	-0.235063353213377
1110	13	-0.7987814777085553
1110	14	-0.5226036143252557
1110	15	-0.13520497707597912
1110	16	-0.5215343348238548
1110	17	-0.37735978789928026
1110	18	-0.30086966428376627
1110	19	-0.3632641946624773
1110	20	-0.04134684138183786
1110	21	-0.21401541136669522
1110	22	-0.20898676251480452
1110	23	-0.02082110117337521
1110	24	0.07926188960361458
1110	25	0.04962102876598351
1111	1	0.18016167743603262
1111	2	0.2635505684924695
1111	3	0.12674398794060338
1111	4	-0.10432351958558428
1111	5	0.1619253610348892
1111	6	0.18349613914516993
1111	7	0.39105840892219135
1111	8	0.6196393449468616
1111	9	1.1682341185698064
1111	10	0.7029727506380112
1111	11	0.9188180893901929
1111	12	0.9454230753177413
1111	13	0.6057272971912795
1111	14	0.45683039052774177
1111	15	0.6151702839138053
1111	16	0.4418142346699625
1111	17	0.602226578558593
1111	18	0.5963991128732786
1111	19	0.7013427688271676
1111	20	0.9605294635670916
1111	21	0.9167080241053398
1111	22	0.9496809198958971
1111	23	1.230411899221636
1111	24	1.1663309037676015
1111	25	1.4162481942758305
1112	1	-0.15719683255462158
1112	2	-0.20453949890186607
1112	3	-0.24813614738320666
1112	4	-0.159547784706497
1112	5	-0.11735837888061695
1112	6	-0.23419381021287933
1112	7	0.05184373246571283
1112	8	-0.5001358866471115
1112	9	0.02668231345811023
1112	10	-0.35434033882671656
1112	11	-0.20995791866440783
1112	12	-0.6827170096979458
1112	13	-0.3037402475443969
1112	14	-1.3729695415351344
1112	15	-0.6357913728956959
1112	16	-0.6892957421738188
1112	17	-0.6029891370860767
1112	18	-0.8280962705369674
1112	19	-0.45908796168544086
1112	20	-0.3321386024445445
1112	21	-0.5420785312762817
1112	22	-0.21685140999309738
1112	23	-0.03066515130844749
1112	24	-0.12562364410781157
1112	25	-0.22710002015777775
1113	1	-2.238160028301138
1113	2	-2.3322094797999213
1113	3	-2.326295670626841
1113	4	-2.2231332725102995
1113	5	-1.9370601200624726
1113	6	-2.105243580901522
1113	7	-2.669341050238664
1113	8	-2.688537617265677
1113	9	-10.929665451966493
1113	10	-4.898506983486538
1113	11	-3.540307123336902
1113	12	-13.150587010842301
1113	13	-5.212929537251352
1113	14	-4.26692844154482
1113	15	-3.8314636160977784
1113	16	-3.4381013204697872
1113	17	-3.899754790557438
1113	18	-3.9625190529068384
1113	19	-4.686881951174971
1113	20	-15.022939079553023
1113	21	-17.14441147728908
1113	22	-12.074616746565837
1113	23	-18.770036943810272
1113	24	-19.734859588333656
1113	25	-16.39881065650781
1114	1	-0.34260387904474854
1114	2	-0.25823250054976654
1114	3	-0.09500169965881256
1114	4	-0.4885464882908721
1114	5	-0.11461043620816724
1114	6	-0.17417002136816961
1114	7	-0.1193488425621112
1114	8	0.005622592904416024
1114	9	0.7439931552113492
1114	10	-0.004921178873480869
1114	11	0.11950229869533757
1114	12	-0.2030939126144986
1114	13	-0.14470774546223453
1114	14	-0.14932001692998398
1114	15	-0.15149990606126806
1114	16	-0.16235882336408025
1114	17	-0.24468532755984285
1114	18	-0.2043204154502547
1114	19	-0.4694427312978675
1114	20	-0.782327716119056
1114	21	-1.0408292348810306
1114	22	-1.0738152504397362
1114	23	-0.5433048216266414
1114	24	-0.10627623273654052
1114	25	-0.461505464594012
1115	1	-0.14937656650503328
1115	2	-0.07538833983061721
1115	3	-0.027871989384102847
1115	4	-0.008105460108346563
1115	5	-0.37869912271651884
1115	6	-0.29365882515037356
1115	7	-0.4442381316323356
1115	8	-0.09486048703071503
1115	9	0.48458511827254114
1115	10	0.05961648492349525
1115	11	0.09899654908727484
1115	12	0.19537637514805084
1115	13	-0.09543432810717434
1115	14	-0.08528187103444651
1115	15	-0.023798988724607284
1115	16	0.07444234839304187
1115	17	0.06445688047611497
1115	18	-0.003285314258626215
1115	19	0.2181414519437439
1115	20	-0.531913454833898
1115	21	8.839446878070589E-4
1115	22	-0.15604433680775287
1115	23	-0.20999119749668438
1115	24	0.3738679306813779
1115	25	0.34255520376878934
1116	1	-0.36488080696943825
1116	2	-0.31640873278203685
1116	3	-0.32816746942548747
1116	4	-0.2917580959656405
1116	5	-1.266359514280314
1116	6	-0.9139904116058268
1116	7	-2.2974539792634587
1116	8	-1.2615484056332988
1116	9	-0.03942199050002415
1116	10	-1.1230441386926877
1116	11	-0.7274699868487318
1116	12	-2.0520538204463286
1116	13	-1.541316429554854
1116	14	-1.0750882080804587
1116	15	-2.2871796216791402
1116	16	-2.547296319363724
1116	17	-3.28998017214915
1116	18	-2.2143791529629944
1116	19	-1.8532699750659312
1116	20	-2.104389978867264
1116	21	-1.5815466892277534
1116	22	-1.5729831767052005
1116	23	-1.7563196364779259
1116	24	-2.298437999686964
1116	25	-1.8772764621470266
1201	1	0.9824956664319586
1201	2	0.9576281412002849
1201	3	0.5086572399313027
1201	4	0.9139948332396628
1201	5	1.0854442799450155
1201	6	1.2316401840070128
1201	7	1.4186555889045493
1201	8	1.4687509483318806
1201	9	2.1392566866243854
1201	10	1.7474492223963694
1201	11	1.6567851270328902
1201	12	1.7906494848793955
1201	13	1.6232930563190209
1201	14	1.7919684672158738
1201	15	1.8917583595992014
1201	16	1.7352975916985756
1201	17	1.6062624537272328
1201	18	1.6415750604448434
1201	19	1.6103642994569398
1201	20	1.8616613623200706
1201	21	1.7550740963033888
1201	22	1.7065156468510025
1201	23	1.975387516486684
1201	24	1.940304007689491
1201	25	2.076652280450382
1202	1	0.7244997526753103
1202	2	0.7778356617596138
1202	3	0.8592425486164643
1202	4	0.9545074629400148
1202	5	0.9323338110073749
1202	6	0.7873185917534968
1202	7	0.7515078491496823
1202	8	0.8443204935646307
1202	9	1.4291939224612236
1202	10	1.2296977925454016
1202	11	1.302722946618779
1202	12	1.244305491813087
1202	13	1.197023123628405
1202	14	1.1714254023551192
1202	15	1.1198855946116666
1202	16	1.116505773988699
1202	17	1.160197412497169
1202	18	1.1408281067459427
1202	19	1.1991058523630969
1202	20	1.3523447720383786
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2211	1	0.11919740638145626
2211	2	0.23740161986705388
2211	3	0.20456916268932446
2211	4	0.13640856152684933
2211	5	-0.4379591999539798
2211	6	0.28027930164731885
2211	7	0.5541350200741064
2211	8	0.4929391911938268
2211	9	1.0378024472335596
2211	10	0.17737192611623698
2211	11	0.781387524240948
2211	12	0.7343736785524158
2211	13	0.5945836807250942
2211	14	0.5406255461641791
2211	15	0.4994557199134846
2211	16	0.5247059545671178
2211	17	0.43821770918506786
2211	18	0.3237424055337854
2211	19	0.3165298317068874
2211	20	0.5397132705001937
2211	21	0.7688949051052631
2211	22	0.5599748694605324
2211	23	0.9712898320334489
2211	24	0.9805655326880753
2211	25	1.1226556805677603
2212	1	-0.15797970927517113
2212	2	-0.16837314114948754
2212	3	-0.12904926031115593
2212	4	0.06281173275542784
2212	5	-0.27684557381397923
2212	6	-0.26461850063130044
2212	7	-0.14880632212996048
2212	8	-0.24958472625337436
2212	9	0.32227227118472257
2212	10	-0.1745691190597327
2212	11	0.04855522286535319
2212	12	-0.025917724155597455
2212	13	-0.3969875501459583
2212	14	-0.37640739148189084
2212	15	-0.1584861046030781
2212	16	-0.07242390401503229
2212	17	-0.26371602967279983
2212	18	-0.35303849756533623
2212	19	-0.13506270687094712
2212	20	0.13169026467137712
2212	21	0.08612138855048102
2212	22	-0.29580294779365346
2212	23	0.044098963307448
2212	24	0.42895427465470704
2212	25	0.41918785367687406
2213	1	-0.07867236803220834
2213	2	-0.23840766638464217
2213	3	-0.2169612261534617
2213	4	-0.15568468443942862
2213	5	-0.024282986109334104
2213	6	0.10713716686100838
2213	7	0.10203374684806477
2213	8	0.3198491028746062
2213	9	0.6589593603116057
2213	10	0.2528717416538768
2213	11	0.4047608905870703
2213	12	0.3283620937350733
2213	13	0.05868832172067329
2213	14	0.23315511097824526
2213	15	0.1685130543016271
2213	16	-0.2862847584065378
2213	17	-0.31101224517865234
2213	18	-0.39306574683116224
2213	19	-0.2715749119291573
2213	20	0.2364871641859665
2213	21	-0.09571988809838672
2213	22	0.1862233794791095
2213	23	-0.22606552962413673
2213	24	-0.09486408063813911
2213	25	0.2633116209820585
2214	1	-0.767109954136135
2214	2	-0.5251887391728438
2214	3	-0.5538543632768338
2214	4	-0.3243493228402059
2214	5	-0.8162191144873105
2214	6	-0.6753998388934406
2214	7	-0.5500025253550263
2214	8	-0.6031775333824996
2214	9	-10.722518119349235
2214	10	-3.57450181751037
2214	11	-1.5224052257357563
2214	12	-1.0276009743224286
2214	13	-0.5826964289704435
2214	14	-0.7994139921560404
2214	15	-0.7766102837874129
2214	16	-0.8923268076571418
2214	17	-0.5314296409487227
2214	18	-0.9673301878871499
2214	19	-1.121213954653058
2214	20	-0.8383769088200665
2214	21	-1.3602636076148968
2214	22	-1.137580277843007
2214	23	-1.525055868673448
2214	24	-2.0660351036525633
2214	25	-9.8522401486054
2215	1	-0.30594152510775174
2215	2	-0.10077296098661392
2215	3	-0.018720272441843377
2215	4	-0.19532950545620764
2215	5	-0.2460438807912837
2215	6	-0.11638639160880615
2215	7	-0.28799216287252705
2215	8	-0.1611138061103503
2215	9	0.685944915338851
2215	10	0.4684063250727917
2215	11	0.6653083173680159
2215	12	0.592294855817336
2215	13	0.5696747419745023
2215	14	0.4291606482301944
2215	15	-0.1342176374807309
2215	16	0.3150311194181157
2215	17	0.19077903649893813
2215	18	0.2259989214230771
2215	19	0.13122349647017728
2215	20	0.2780046606186417
2215	21	0.371512628167352
2215	22	0.2791507467794432
2215	23	0.7175354235126434
2215	24	0.9044332324765065
2215	25	0.9761421235394536
2216	1	-0.422436323537827
2216	2	-0.3407543013195138
2216	3	-0.4849878556348166
2216	4	-0.23163185025738642
2216	5	-0.9809803999092317
2216	6	-1.349486745044306
2216	7	-0.7035419167441965
2216	8	-1.6395664898219522
2216	9	-0.3957420480959897
2216	10	-0.9834948162361705
2216	11	-0.43338330018952576
2216	12	-1.5416654567227006
2216	13	-1.174455746166327
2216	14	-1.107925909441363
2216	15	-0.9209802556347668
2216	16	-1.9988851626901472
2216	17	-2.444500264833568
2216	18	-1.8285693414679791
2216	19	-3.1191009123548668
2216	20	-2.102135136706213
2216	21	-2.1106987394933263
2216	22	-1.9535197209652857
2216	23	-1.690216081908563
2216	24	-1.6223053289739156
2216	25	-1.790324821489147
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42974.0
3	3.0
4	588.0
5	6238.0
6	9139.0
7	7171.0
8	5699.0
9	4441.0
10	3753.0
11	3401.0
12	2988.0
13	2790.0
14	2823.0
15	2669.0
16	2863.0
17	3120.0
18	3462.0
19	4683.0
20	6468.0
21	8552.0
22	10199.0
23	11801.0
24	13630.0
25	14982.0
26	16816.0
27	18450.0
28	20020.0
29	23220.0
30	28100.0
31	34500.0
32	42663.0
33	52893.0
34	66630.0
35	83788.0
36	110801.0
37	169350.0
38	391596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.46574561392836	18.948656744018553	13.11186216327355	22.47373547877953
2	15.254645471617945	22.259017680199538	40.838876978116254	21.647459870066264
3	18.072669185735368	29.927590289152327	29.90107519906589	22.09866532604641
4	11.151430621834974	18.119777328181446	38.281064381023434	32.44772766896015
5	11.263641333707538	40.1601513261315	34.30396955307852	14.272237787082446
6	26.833204391483672	38.80890169116562	20.281012136752967	14.076881780597738
7	25.13390061994933	34.20378198286461	23.34274665775618	17.319570739429878
8	25.95617356998773	34.54245203822829	21.531191677569065	17.970182714214918
9	27.02229605481421	15.644064538265553	21.168070900918952	36.165568506001286
10	15.089356186063338	31.645093176871793	32.49740623299044	20.768144404074423
11	32.20758045872118	24.334692056539108	24.438928433818013	19.0187990509217
12	22.368730486964278	28.440741191258162	30.918055386611528	18.272472935166036
13	27.66245739849225	23.359046277623577	27.821065153243797	21.157431170640375
14	21.280517422714603	22.513337371643765	31.194326254937405	25.011818950704228
15	22.78958012685881	29.36898203184931	27.201662041774853	20.63977579951702
16	20.537039995393975	29.328712169560166	28.608128501654683	21.52611933339118
17	20.196797459506012	28.396232076331597	29.720000713621907	21.686969750540488
18	19.255048764307737	26.528172680633926	33.51169516184754	20.70508339321079
19	20.986010299663434	26.32764283686793	33.467661489801706	19.218685373666926
20	21.313024247471972	25.6346521750061	33.325169474412384	19.727154103109537
21	22.36803905508746	25.080810620129263	32.983545935951604	19.567604388831672
22	21.234218095799577	25.72666297418697	32.98198010440041	20.05713882561305
23	20.406319663111322	25.593613841434294	33.56795957076373	20.432106924690654
24	20.13725245336936	25.830642506256073	33.754324109107834	20.277780931266733
25	20.449933439397384	25.302444884574175	33.69955193350434	20.54806974252411
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1670.0
1	1670.0
2	2525.5
3	3381.0
4	3381.0
5	3381.0
6	11790.5
7	20200.0
8	20200.0
9	20200.0
10	16744.5
11	13289.0
12	13289.0
13	13289.0
14	12098.5
15	10908.0
16	10908.0
17	10908.0
18	17629.5
19	24351.0
20	24351.0
21	24351.0
22	34785.5
23	45220.0
24	45220.0
25	45220.0
26	63848.0
27	82476.0
28	82476.0
29	82476.0
30	92851.0
31	103226.0
32	103226.0
33	103226.0
34	110032.0
35	116838.0
36	116838.0
37	116838.0
38	120107.5
39	123377.0
40	123377.0
41	123377.0
42	130008.5
43	136640.0
44	136640.0
45	136640.0
46	141986.5
47	147333.0
48	147333.0
49	147333.0
50	142081.0
51	136829.0
52	136829.0
53	136829.0
54	120978.5
55	105128.0
56	105128.0
57	105128.0
58	89289.0
59	73450.0
60	73450.0
61	73450.0
62	59534.0
63	45618.0
64	45618.0
65	45618.0
66	34398.5
67	23179.0
68	23179.0
69	23179.0
70	17103.0
71	11027.0
72	11027.0
73	11027.0
74	7819.0
75	4611.0
76	4611.0
77	4611.0
78	3646.5
79	2682.0
80	2682.0
81	2682.0
82	1856.0
83	1030.0
84	1030.0
85	1030.0
86	742.5
87	455.0
88	455.0
89	455.0
90	386.0
91	317.0
92	317.0
93	317.0
94	172.5
95	28.0
96	28.0
97	28.0
98	14.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026515004086716226
2	0.0016217127881783624
3	3.243425576356725E-4
4	0.004784052725126169
5	0.03486682494583479
6	0.012162845911337717
7	0.02108226624631871
8	0.028785401990165934
9	0.03608310953696857
10	0.04062390534386798
11	0.03965087767096096
12	0.009243762892616665
13	0.002675826100494298
14	0.006081422955668859
15	0.0066490224315312865
16	0.007865307022665058
17	0.009568105450252338
18	0.015406271487694443
19	0.01735232683350848
20	0.01516301456946769
21	0.01086547568079503
22	0.010297876204932601
23	0.007865307022665058
24	0.005027309643352924
25	0.10573567378922924
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1233264.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.02406224425426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56723104703687	44.331363359793706
2	11.335848117596859	12.474888248281239
3	3.496220799305047	5.7712881264185185
4	1.4467695623428878	3.1842855380579014
5	0.7817788168857396	2.1508323140780194
6	0.48488085775429496	1.600806869887183
7	0.3120325674559107	1.2018509589749946
8	0.2274062578422958	1.001025286899793
9	0.16668610301986575	0.8254571857035544
>10	1.0322450448529674	10.892284025888403
>50	0.09542292369430813	3.6558004298966544
>100	0.048776553120701	4.732445431699813
>500	0.002644508864388548	1.003140942801248
>1k	0.0011753372730615769	1.201440945435744
>5k	2.938343182653942E-4	1.0413781630401868
>10k+	5.876686365307884E-4	4.931712173143117
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	18170	1.4733260680600424	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	17184	1.393375627602849	No Hit
TATCAACGCAGAGTACTTTTTTTTT	13508	1.095304817135666	No Hit
GGTATCAACGCAGAGTACTTTTTTT	12144	0.9847040049819017	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	7655	0.6207105696752683	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5227	0.42383463719041503	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2627	0.2130119747272279	No Hit
GTATAACGCAGAGTACTTTTTTTTT	2337	0.18949713929864165	No Hit
GTGGTATCAACGCAGAGTACATGGG	2177	0.17652343699321474	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2080	0.1686581299705497	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1945	0.15771156865034575	No Hit
GGTATCAACGCAGAGTACATGGGAT	1423	0.11538486487889048	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	8.108563940891812E-5	0.0	0.0	0.0	0.0
7	3.243425576356725E-4	0.0	0.0	0.0	0.0
8	3.243425576356725E-4	0.0	0.0	8.108563940891812E-5	0.0
9	3.243425576356725E-4	0.0	0.0	8.108563940891812E-5	0.0
10	3.243425576356725E-4	0.0	0.0	8.108563940891812E-5	0.0
11	3.243425576356725E-4	0.0	0.0	8.108563940891812E-5	0.0
12	3.243425576356725E-4	0.0	0.0	8.108563940891812E-5	0.0
13	3.243425576356725E-4	0.0	0.0	8.108563940891812E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAA	55	5.924394E-7	17.267935	14
ACGGCCC	40	2.7663866E-4	16.620386	15
TATAACG	380	0.0	16.499437	2
TATCACG	205	0.0	16.218958	2
ATAACGC	385	0.0	16.038412	3
TAACGCA	395	0.0	15.874807	4
TTTTCCG	50	8.6810396E-5	15.206266	5
GCGCCAA	45	6.760596E-4	14.777873	11
ACAACGC	110	1.8553692E-10	13.81771	3
TCACGCA	245	0.0	13.572618	4
GGTATCA	3120	0.0	13.338788	1
GGGCGTA	50	0.0014973361	13.302244	9
GGCGTAG	50	0.001497794	13.301703	10
GTAAGAC	50	0.0014996266	13.299546	3
CGCCAAC	50	0.0015032976	13.295231	12
GTGTTAT	115	3.765308E-10	13.219621	1
TACAACG	115	3.765308E-10	13.216939	2
TGGTCGG	65	5.444156E-5	13.155532	10
GTATAAC	515	0.0	13.099297	1
ATCACGC	255	0.0	13.038771	3
>>END_MODULE