FastQCFastQC Report
Thu 26 May 2016
SRR1364288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301021
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT157840.68595636458772No Hit
TATCAACGCAGAGTACTTTTTTTTT94780.41190410691601687No Hit
GGTATCAACGCAGAGTACTTTTTTT73380.31890191354185815No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN62170.2701844094425909No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38130.1657090482876949No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA36520.1587121543001998No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35290.15336670112962897No Hit
GTGGTATCAACGCAGAGTACATGGG32410.14085051809609733No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25680.11160263204899043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG902.2610038E-914.79072714
TATAACG1450.014.4037652
CGACGGT1101.8371793E-1013.829988515
GGTATCA35050.013.3798361
CTCGACG957.5157004E-812.98359213
CGCATAT2150.012.3718518
GTTATCA5450.012.3672671
GCTCGAG701.1042785E-412.1988711
ATAACGC1650.012.0824973
GTGGTAT10800.011.8664781
TGGTATC10850.011.7245562
ATCACGC1302.6466296E-911.6841733
CGAGAGC1156.9934686E-811.57535114
ACGGTCA1451.200533E-911.14744717
TACGATC600.00585602211.0886844
TAACGCA1803.6379788E-1211.0886834
TACACCG600.00585697411.0884435
TATCACG1201.2884811E-711.0756222
GTATCAA87800.011.0501531
ATCCGAC700.001481008710.86665616
Produced by FastQC (version 0.11.5)