##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364288_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2301021
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.64411798067032	31.0	26.0	31.0	10.0	34.0
2	26.422266898042217	31.0	26.0	31.0	10.0	34.0
3	25.93777371001829	30.0	25.0	31.0	10.0	34.0
4	29.713652765446295	35.0	28.0	37.0	10.0	37.0
5	30.324027029740275	35.0	30.0	37.0	10.0	37.0
6	30.434639231888802	35.0	31.0	37.0	8.0	37.0
7	30.02661383794411	35.0	30.0	37.0	7.0	37.0
8	29.5517468115241	35.0	29.0	37.0	2.0	37.0
9	30.579886928454805	37.0	29.0	39.0	2.0	39.0
10	29.94585620904807	35.0	27.0	39.0	2.0	39.0
11	30.57331853990033	37.0	30.0	39.0	2.0	39.0
12	30.122865458420414	35.0	27.0	39.0	2.0	39.0
13	29.707000066492224	35.0	27.0	39.0	2.0	39.0
14	30.04369973155395	36.0	27.0	40.0	2.0	41.0
15	30.77633233247328	36.0	27.0	40.0	2.0	41.0
16	31.127025785509996	37.0	30.0	40.0	2.0	41.0
17	31.329139108248036	37.0	30.0	40.0	2.0	41.0
18	31.182994853154316	37.0	30.0	40.0	2.0	41.0
19	31.179969674331524	38.0	30.0	40.0	2.0	41.0
20	31.084089193449344	38.0	30.0	40.0	2.0	41.0
21	30.996535451001968	38.0	29.0	40.0	2.0	41.0
22	31.091995249065523	38.0	30.0	40.0	2.0	41.0
23	31.104995130422537	38.0	30.0	40.0	2.0	41.0
24	30.980589920735188	38.0	30.0	40.0	2.0	41.0
25	30.813633600041026	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9747350339332073
1101	2	1.132158508791715
1101	3	1.8070704142602203
1101	4	1.186339642461114
1101	5	0.7855050481307551
1101	6	0.7512976895548427
1101	7	1.0053943031703483
1101	8	1.1913804178959992
1101	9	1.1976432562639374
1101	10	1.4687979518642962
1101	11	0.8756405489788612
1101	12	1.227672647717057
1101	13	1.1703744356710182
1101	14	1.7447789522048112
1101	15	1.0452150327203213
1101	16	0.5352138361607928
1101	17	-0.004806937198228667
1101	18	-5.992905616291807
1101	19	-7.190346425495989
1101	20	-8.236407072737872
1101	21	-9.911606589881856
1101	22	-9.952431878448849
1101	23	-11.551648219020425
1101	24	-10.987560780898882
1101	25	-11.58631943250921
1102	1	1.916024465469615
1102	2	2.231111705109001
1102	3	2.812369820319102
1102	4	2.204959285615356
1102	5	1.6195183301214904
1102	6	1.639341692914428
1102	7	1.950541886413312
1102	8	2.1891754390537734
1102	9	2.3864644144390965
1102	10	-1.2731338902351652
1102	11	1.1783634573058634
1102	12	2.121857156337523
1102	13	2.54954965331245
1102	14	3.0545903463327555
1102	15	2.570042092220671
1102	16	2.245696826753548
1102	17	1.9080444412142263
1102	18	2.094859036293734
1102	19	1.9206406633506248
1102	20	2.116008120769063
1102	21	2.330723230853362
1102	22	2.050000413666748
1102	23	1.9871516711315351
1102	24	2.208505617027633
1102	25	2.2154078276442206
1103	1	-0.32004977878601437
1103	2	0.07569316149659855
1103	3	-2.122270326670165
1103	4	-0.14811507134790958
1103	5	-2.9339835887177728
1103	6	-7.6595354944317755
1103	7	-7.896901881234442
1103	8	-2.108917422123799
1103	9	-6.975771321682245
1103	10	-4.202923973086847
1103	11	-8.544201570208351
1103	12	-1.911769051592536
1103	13	-0.0027035574504985505
1103	14	0.40818593480378595
1103	15	0.2574836519886077
1103	16	0.23798246823022495
1103	17	-0.05550835471190396
1103	18	0.2635856746611971
1103	19	-5.01998125144306
1103	20	-1.1190662976710932
1103	21	-5.905275654630056
1103	22	-2.036093347849839
1103	23	-1.0749191680974626
1103	24	-6.303389996529937
1103	25	-2.7067802738005895
1104	1	1.5522248009135922
1104	2	1.6059512133215605
1104	3	2.4114619964718393
1104	4	1.8661724596244689
1104	5	1.4602145669478617
1104	6	1.2652326722219769
1104	7	1.491368056830737
1104	8	1.7449456059465653
1104	9	1.9081907981299437
1104	10	2.158538340457941
1104	11	1.7348061612126955
1104	12	2.0470801568856025
1104	13	2.2619629939701085
1104	14	2.8047148426884085
1104	15	1.8498195902803012
1104	16	0.9929455000758942
1104	17	0.9677236614384377
1104	18	1.0261728294942785
1104	19	1.3673925491534362
1104	20	-9.57749227492788
1104	21	-3.6087932425697566
1104	22	-0.9455626000022583
1104	23	0.2415932079385108
1104	24	0.7705622081470658
1104	25	1.217293435989962
1105	1	1.487332659114088
1105	2	1.5660907125294408
1105	3	2.188511931517173
1105	4	1.6316603046189755
1105	5	1.4110980858261328
1105	6	1.342422393215596
1105	7	1.5613470530959432
1105	8	1.8012224729253
1105	9	1.6557616688092196
1105	10	2.2252966844046576
1105	11	1.898230271779795
1105	12	2.0966889553556776
1105	13	2.2175596489839613
1105	14	2.801623542153397
1105	15	2.122668619066655
1105	16	1.5086745654375697
1105	17	1.0989864104346516
1105	18	1.5960265361317596
1105	19	1.5529325490419268
1105	20	1.6069008429837588
1105	21	1.4770353296780776
1105	22	1.4253839858354773
1105	23	1.347469793274449
1105	24	1.2383031955376538
1105	25	1.188032931533936
1106	1	0.848107300181816
1106	2	1.204424182846882
1106	3	1.6688077032652977
1106	4	1.3414862154480183
1106	5	0.5409605673434505
1106	6	0.3909977720444715
1106	7	0.9827668649041925
1106	8	0.9483150869320198
1106	9	1.19509387534654
1106	10	-2.1569353645059977
1106	11	-0.13323484243647243
1106	12	0.48740802427604635
1106	13	1.4220849258071482
1106	14	0.48277523401564437
1106	15	1.066233471819583
1106	16	0.6970257351869229
1106	17	-6.2494003372072555
1106	18	-1.9934720450655021
1106	19	-0.4800595175437401
1106	20	0.3120871427283163
1106	21	0.4706513276859745
1106	22	0.6365924867708976
1106	23	0.12801205504212732
1106	24	0.502116442524887
1106	25	0.714291483207731
1107	1	0.8764935819225954
1107	2	1.1798384359961993
1107	3	1.5814899390582262
1107	4	1.114448786991904
1107	5	0.4401835819835149
1107	6	0.7075537575406585
1107	7	0.7884562646683442
1107	8	0.9048780556976723
1107	9	0.9423942200327318
1107	10	-2.1676750402671274
1107	11	-0.011910198678236839
1107	12	0.40805514916931074
1107	13	1.2320791182264976
1107	14	1.496530310011849
1107	15	1.3008503889187075
1107	16	-5.658662972048202
1107	17	-1.8045781512828611
1107	18	-0.09113748139261091
1107	19	0.7302908602711078
1107	20	1.0742010054523128
1107	21	1.2488745429984043
1107	22	0.7947767465201778
1107	23	0.4464261120285684
1107	24	0.7017086234534098
1107	25	1.0365117119484601
1108	1	-0.5213803169608617
1108	2	-3.3576742599496647
1108	3	-0.3628489364961567
1108	4	-0.7862448792825951
1108	5	-1.0850186353710782
1108	6	-0.5324289008024792
1108	7	-0.21774180432417722
1108	8	0.10404313883098837
1108	9	-0.4694456563380385
1108	10	-3.7336301512484837
1108	11	-1.5679113384726193
1108	12	-5.878731627118071
1108	13	-1.6757277740332128
1108	14	-5.899852416159543
1108	15	-2.706590615359019
1108	16	-7.514055056071328
1108	17	-3.2892232679107494
1108	18	-1.9236856036163275
1108	19	-0.5912185631362838
1108	20	-0.5089471695655625
1108	21	-0.005472671235740023
1108	22	-0.3337333219548917
1108	23	-0.2033615750454274
1108	24	-0.09167042985295382
1108	25	-0.177010934472289
1109	1	-9.30667867005506
1109	2	-6.673932950911535
1109	3	-5.3516551766955835
1109	4	-5.040471640828482
1109	5	-2.4386145299931528
1109	6	-1.4729651747722805
1109	7	-1.1161048137635667
1109	8	-0.7150963978774563
1109	9	-5.5243016843161925
1109	10	-1.434219639610376
1109	11	-1.0539664117770826
1109	12	-6.016735298879897
1109	13	-7.950106910181564
1109	14	-8.212306231243435
1109	15	-3.966419487680003
1109	16	-2.327686013169174
1109	17	-1.9807344559322004
1109	18	-1.176013011097183
1109	19	-0.988265382979101
1109	20	-0.7744260112625021
1109	21	-0.5732368196785984
1109	22	-0.8407185306804621
1109	23	-1.1945227238548384
1109	24	-0.8773904539149768
1109	25	-0.7969190194138882
1110	1	-2.5753293191520257
1110	2	-2.4601446274920775
1110	3	-1.8385036191720872
1110	4	-2.5672553282181347
1110	5	-5.567278799831659
1110	6	-4.091288013497724
1110	7	-8.867655172420466
1110	8	-9.697075538383022
1110	9	-11.172275881835045
1110	10	-8.878907361861884
1110	11	-11.833903346084533
1110	12	-6.581679009619393
1110	13	-4.843519102027372
1110	14	-4.421490955197161
1110	15	-9.754727069063179
1110	16	-11.296072091051762
1110	17	-11.909116129322506
1110	18	-8.823573910367802
1110	19	-12.175164808962133
1110	20	-13.134184563632594
1110	21	-14.11082214728911
1110	22	-14.756241424612895
1110	23	-15.500669856522821
1110	24	-16.043165210894855
1110	25	-16.42022987022755
1111	1	-0.17577900770455557
1111	2	0.5315475046372917
1111	3	-2.1117387550890214
1111	4	-2.7149043460966844
1111	5	-0.6266321803313275
1111	6	-0.15213691920451922
1111	7	0.4573654326589569
1111	8	0.8418925109301334
1111	9	-3.732087758490888
1111	10	-3.458273296878854
1111	11	-0.7486335182245796
1111	12	-5.443393004636469
1111	13	-0.5960180754555076
1111	14	0.8335938894208041
1111	15	0.8246935084446179
1111	16	0.6411693144650386
1111	17	0.6222756134459786
1111	18	0.8998621803051563
1111	19	1.1300385406372797
1111	20	1.1623321027689606
1111	21	1.3114090690969213
1111	22	0.8389646468011591
1111	23	0.7241433427464123
1111	24	1.0242643592057163
1111	25	1.2193666708291389
1112	1	0.16252191949768857
1112	2	0.6745288394315807
1112	3	-2.069822962448459
1112	4	-0.1406304184431626
1112	5	-0.18353226011017298
1112	6	-0.0035690167559607744
1112	7	0.5770354537830862
1112	8	-2.94345045322558
1112	9	-0.3064082189254407
1112	10	-3.077727628319856
1112	11	-0.5659480148540261
1112	12	0.6038818132857351
1112	13	1.038177489824129
1112	14	1.7889851949901647
1112	15	1.2075577679324745
1112	16	0.8743586888361854
1112	17	0.7425694372289691
1112	18	0.7837469417266085
1112	19	0.92677735360067
1112	20	1.0729958943536069
1112	21	0.9391327732467651
1112	22	0.841350988288557
1112	23	0.617186282260235
1112	24	0.7988275217491108
1112	25	0.696490248433232
1113	1	-1.2331565599514036
1113	2	-0.7475412687583933
1113	3	-2.9668962691986778
1113	4	-1.6266428275817333
1113	5	-1.6860063092664106
1113	6	-1.48369203699594
1113	7	-1.2909107644985696
1113	8	-8.697422937531382
1113	9	-10.023807026468177
1113	10	-3.7032408325291186
1113	11	-9.375973939131672
1113	12	-7.851067368924234
1113	13	-3.271501162198444
1113	14	-1.5730703592193755
1113	15	-7.659492640951914
1113	16	-3.4043508865692864
1113	17	-2.4645416954184043
1113	18	-1.8964633428878699
1113	19	-1.5940428295057458
1113	20	-7.17358292135874
1113	21	-2.9579900527417777
1113	22	-2.16179741513044
1113	23	-2.1374501423912484
1113	24	-7.616087800353345
1113	25	-4.665299219568624
1114	1	-4.290474387570686
1114	2	-4.301145916161474
1114	3	-1.1309506757171128
1114	4	-0.8765045479399909
1114	5	-0.8893978788427326
1114	6	-0.9432583368569034
1114	7	-0.676409185545527
1114	8	-0.3605659701990014
1114	9	-0.5123715264304529
1114	10	-3.5482120018165944
1114	11	-1.3790081156510787
1114	12	-4.984995135864299
1114	13	-1.667230767837406
1114	14	-6.32054478880238
1114	15	-2.508546670246073
1114	16	-1.4400694988150633
1114	17	-1.3197453426382602
1114	18	-0.9166840729785122
1114	19	-0.5419104345627055
1114	20	-0.41787745807344123
1114	21	-0.18500168202731615
1114	22	-0.18637885785162567
1114	23	-0.6880727780033737
1114	24	-0.5584802644900932
1114	25	-0.3683890097241509
1115	1	0.3275941665904014
1115	2	0.40657010552952144
1115	3	0.9810444003843948
1115	4	-0.07185325828771383
1115	5	-0.1846754582370096
1115	6	-0.8967060347908067
1115	7	-2.7293701640088557
1115	8	-0.6885622090870349
1115	9	-0.4153938382941078
1115	10	0.39141253297907497
1115	11	-0.4357512367280201
1115	12	0.0654207756490841
1115	13	0.4073614852768159
1115	14	1.0184858635423915
1115	15	0.04228418379192078
1115	16	-0.24975594122662415
1115	17	-0.6137483070261283
1115	18	-0.36310157794009257
1115	19	-0.02031813479347022
1115	20	-0.05892360706084787
1115	21	0.03656348605634818
1115	22	-0.38361121059224956
1115	23	-0.4800583795480158
1115	24	-0.5888060241593429
1115	25	-0.24332823259515735
1116	1	0.17932232358975497
1116	2	0.3987920164844958
1116	3	1.143600686843861
1116	4	0.45267893776261303
1116	5	-0.4093786014527758
1116	6	-1.174261053216398
1116	7	-4.772349250387656
1116	8	-1.2103564016036223
1116	9	-1.37915760581825
1116	10	-0.6499896108360765
1116	11	-2.28173309155434
1116	12	-0.7847927232697387
1116	13	-6.730146034946092
1116	14	-1.8811844476509947
1116	15	-1.6328808700883464
1116	16	-1.0849494532503243
1116	17	-0.7823175598301866
1116	18	-0.692928778998315
1116	19	-0.3629885079920392
1116	20	-0.27539067476882906
1116	21	-0.1073012409840004
1116	22	-0.34706802454263297
1116	23	-0.4239723895347609
1116	24	-0.2490306923897343
1116	25	-0.14420771463404236
1201	1	2.3785957199360688
1201	2	2.6178917872415433
1201	3	3.128829553490256
1201	4	2.6345629937544572
1201	5	2.1857587409920107
1201	6	2.211321404144229
1201	7	2.341302328244737
1201	8	2.3966262980492665
1201	9	2.856498853255175
1201	10	3.0807351962617737
1201	11	2.541403194200001
1201	12	2.9292788913301955
1201	13	3.035813268208745
1201	14	3.685467166791131
1201	15	3.175075960374439
1201	16	2.7090371071767727
1201	17	2.3780779282655082
1201	18	2.4997395434130496
1201	19	2.473979664443334
1201	20	2.8081719318637433
1201	21	2.565262347077887
1201	22	1.751309415450276
1201	23	1.2636808260270556
1201	24	1.7687426921211653
1201	25	1.8431709495660016
1202	1	2.1650260443681013
1202	2	2.428947104941823
1202	3	3.0345417345013246
1202	4	2.698251869436291
1202	5	2.135478555811101
1202	6	2.0227005672526843
1202	7	2.204477523080911
1202	8	2.505111865170612
1202	9	2.7397255363857838
1202	10	-0.9959762557966023
1202	11	1.455132171896743
1202	12	2.503352698271204
1202	13	2.9999594703459813
1202	14	3.6683290850714947
1202	15	3.049612190669638
1202	16	2.6800941751328544
1202	17	2.3346662950268033
1202	18	2.5091022098796927
1202	19	2.7135466492334146
1202	20	2.6491330627213294
1202	21	2.8492288498744465
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2211	15	-1.5306680173978222
2211	16	-0.6323866228214463
2211	17	-0.1718133614397388
2211	18	0.25969865496065836
2211	19	0.5309632591801972
2211	20	0.7440026896935272
2211	21	0.8693833170383591
2211	22	0.6736999128652172
2211	23	0.6455860355486394
2211	24	0.7394254118887531
2211	25	0.8029809970317316
2212	1	1.2384212998442834
2212	2	1.240326452628974
2212	3	1.8395917266900454
2212	4	1.3288601609911126
2212	5	0.9827365304173377
2212	6	0.8513264211675562
2212	7	1.0268898592215656
2212	8	1.3557989618837212
2212	9	1.1682455237930576
2212	10	2.017788905186766
2212	11	1.1997741963436823
2212	12	1.476399345812073
2212	13	1.7104003697319676
2212	14	-4.226629917546468
2212	15	-0.7770553695600597
2212	16	-0.08220311624680221
2212	17	0.35867503182455707
2212	18	0.7236172536938668
2212	19	1.1483803104037875
2212	20	1.3597472009004967
2212	21	1.2944090537251718
2212	22	1.2276956747896968
2212	23	1.1477672438798834
2212	24	1.245844557888418
2212	25	1.2820442643420336
2213	1	0.9307918101338686
2213	2	1.0689549011996178
2213	3	1.5854772854368768
2213	4	1.143569783504205
2213	5	0.7767857283682709
2213	6	0.34260701215912803
2213	7	0.6168995605029437
2213	8	-4.741553068994321
2213	9	-1.2911563069960579
2213	10	0.40793486686451885
2213	11	0.3024338004483731
2213	12	0.8602913677098769
2213	13	-7.9800734471869355
2213	14	-1.9051289883865792
2213	15	-0.520662410854964
2213	16	0.11761583140451393
2213	17	0.20676435462486253
2213	18	0.4530341225616006
2213	19	0.807643149579917
2213	20	1.0654315727698354
2213	21	1.014163014808613
2213	22	0.7378768309246091
2213	23	0.6126312347554332
2213	24	0.6722248874479355
2213	25	0.8250992994490893
2214	1	-1.1357319160975834
2214	2	-0.698766529882981
2214	3	-6.969407935419117
2214	4	-2.632057497864203
2214	5	-1.6686513177994655
2214	6	-1.2030301216825983
2214	7	-1.0146445217196352
2214	8	-6.476415199472317
2214	9	-2.2685911048901453
2214	10	-0.4611644998862019
2214	11	-0.9022806297357882
2214	12	-0.4310863075404505
2214	13	-6.641141312064821
2214	14	-1.8814320539380809
2214	15	-1.1450119088996615
2214	16	-1.0817589488679715
2214	17	-1.0808589640527373
2214	18	-0.8472726279017238
2214	19	-0.6002904786042329
2214	20	-0.37658152190505234
2214	21	-0.2108602057782818
2214	22	-0.43069106650549926
2214	23	-0.5938030379627648
2214	24	-0.39012510105027687
2214	25	-0.25052874147122495
2215	1	0.34744040093976025
2215	2	0.6691766502977714
2215	3	-2.4073534110455057
2215	4	-0.39472629130872505
2215	5	0.08429268896511743
2215	6	-0.40018600310579444
2215	7	0.16059837105568775
2215	8	0.5884515250552163
2215	9	0.9097479866048666
2215	10	1.4503913096018621
2215	11	0.8019785668470725
2215	12	0.47085320439530776
2215	13	1.0691619995204213
2215	14	1.12223744768772
2215	15	0.673562435055274
2215	16	0.5401603508591464
2215	17	0.35676132344205413
2215	18	0.5106300720114838
2215	19	0.3720461901065555
2215	20	0.6553968003755166
2215	21	0.808742536916224
2215	22	0.5831694318351879
2215	23	0.43184982363694147
2215	24	0.4604845324491542
2215	25	0.45490247279159135
2216	1	0.09397386010837039
2216	2	-0.15603823417087526
2216	3	0.999419347963844
2216	4	-0.5891705765946469
2216	5	-0.6645756257289328
2216	6	-0.9423045321863768
2216	7	-1.5458411918604504
2216	8	-6.555815807785361
2216	9	-2.3611264948907724
2216	10	-0.9871000446798242
2216	11	-1.515094540664876
2216	12	-0.35357616455388197
2216	13	0.20388391365721859
2216	14	0.9992614325132756
2216	15	0.5083651653424255
2216	16	-0.049258408582932844
2216	17	-0.24729656405869704
2216	18	-0.12047134952877414
2216	19	0.009978104273365318
2216	20	0.1871610671408419
2216	21	-0.04245056538987413
2216	22	-0.4128998528709431
2216	23	-0.5463731978339048
2216	24	-0.5280163552461374
2216	25	-0.3772258486307756
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	193740.0
3	7.0
4	1171.0
5	9119.0
6	15096.0
7	13839.0
8	12350.0
9	9965.0
10	8483.0
11	7752.0
12	7159.0
13	6720.0
14	6878.0
15	6905.0
16	6868.0
17	7408.0
18	8626.0
19	11638.0
20	16199.0
21	20539.0
22	25477.0
23	29362.0
24	33751.0
25	37884.0
26	41845.0
27	45494.0
28	49653.0
29	57385.0
30	66016.0
31	74138.0
32	93091.0
33	118369.0
34	149265.0
35	187989.0
36	237734.0
37	284993.0
38	398113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.9404790506368	18.422515632828134	13.507434995297398	22.12957032123767
2	14.117920992699432	21.173000759140535	41.71610236929462	22.992975878865415
3	15.868711051803011	27.801806280553482	31.12458626058061	25.2048964070629
4	10.376273185095252	17.141270995888476	37.82927501495813	34.653180804058145
5	12.034209504869315	40.19634267073133	33.28998268590842	14.479465138490927
6	30.086507535574793	37.716622985017764	18.003697067147403	14.193172412260036
7	27.93135165060309	34.37761823182986	20.479771230291064	17.211258887275992
8	25.943101994023817	35.811133317786236	19.67861104875322	18.567153639436732
9	27.16017631530309	17.289605557279806	19.562507192450802	35.987710934966294
10	13.024285065324124	32.683619505029945	32.14490657727247	22.147188852373457
11	33.123840509966385	25.623596406801415	21.940166827077316	19.312396256154887
12	22.475829301251036	28.964125365066913	29.219040146462667	19.34100518721939
13	26.966231406875064	23.80116133205362	26.8646736946347	22.367933566436616
14	20.47700996110062	23.453483769839817	29.190575574816357	26.87893069424321
15	23.301959820284427	30.58564472177038	24.301004405716448	21.81139105222875
16	21.94202772208177	31.213582076540842	25.374509632987536	21.46988056838985
17	21.05003251468391	30.65507063444604	26.27705125813521	22.01784559273484
18	20.63287690936392	28.80792926772393	29.325999167496626	21.23319465541552
19	21.975555740556626	28.31571198122636	29.53648659427507	20.17224568394194
20	22.604582912992782	27.58582589821863	29.246094654141608	20.56349653464698
21	23.425839155809907	26.925034978446305	29.02853631002454	20.62058955571925
22	22.64953614026156	27.304043015776987	28.86413207405716	21.182288769904297
23	22.03095368540982	27.648669699358987	28.867979380481717	21.452397234749476
24	21.733496292021503	27.698734483982072	29.31338542315937	21.25438380083705
25	21.819970328323553	27.371380604625784	29.372649757414	21.43599930963667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10843.0
1	10843.0
2	9062.5
3	7282.0
4	7282.0
5	7282.0
6	10652.0
7	14022.0
8	14022.0
9	14022.0
10	15282.0
11	16542.0
12	16542.0
13	16542.0
14	19576.5
15	22611.0
16	22611.0
17	22611.0
18	32548.5
19	42486.0
20	42486.0
21	42486.0
22	58369.0
23	74252.0
24	74252.0
25	74252.0
26	99687.5
27	125123.0
28	125123.0
29	125123.0
30	149188.5
31	173254.0
32	173254.0
33	173254.0
34	188974.0
35	204694.0
36	204694.0
37	204694.0
38	218683.5
39	232673.0
40	232673.0
41	232673.0
42	249529.0
43	266385.0
44	266385.0
45	266385.0
46	276801.0
47	287217.0
48	287217.0
49	287217.0
50	276689.5
51	266162.0
52	266162.0
53	266162.0
54	239228.5
55	212295.0
56	212295.0
57	212295.0
58	182436.5
59	152578.0
60	152578.0
61	152578.0
62	123387.5
63	94197.0
64	94197.0
65	94197.0
66	72417.0
67	50637.0
68	50637.0
69	50637.0
70	38025.0
71	25413.0
72	25413.0
73	25413.0
74	18445.0
75	11477.0
76	11477.0
77	11477.0
78	8938.0
79	6399.0
80	6399.0
81	6399.0
82	4524.0
83	2649.0
84	2649.0
85	2649.0
86	1967.5
87	1286.0
88	1286.0
89	1286.0
90	877.0
91	468.0
92	468.0
93	468.0
94	259.5
95	51.0
96	51.0
97	51.0
98	38.0
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33072275307352694
2	0.3319396042017869
3	0.34380390270232214
4	0.34562917939471216
5	0.3516265170982794
6	0.29421721922572636
7	0.3046908307225358
8	0.300562228680225
9	0.3019963746528172
10	0.4106003378500239
11	0.30025801589816
12	0.30077952352455717
13	0.2937391705681956
14	0.29973650827176285
15	0.2924354015022027
16	0.29643362663791417
17	0.2900016992456827
18	0.29273961428426776
19	0.2929134501597334
20	0.48721849996153876
21	0.2933914988172642
22	0.2952167755096542
23	0.29074050171641197
24	0.2946083499455242
25	0.28582963823450547
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2301021.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.50206157203638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.12423958666142	50.055656835862685
2	7.948637820917037	9.459206740680454
3	2.989938055412419	5.337224348091734
4	1.515548459610872	3.607130310366839
5	0.8620031315123187	2.5645481703267077
6	0.5653714400673696	2.0184459742775704
7	0.38883798634852595	1.6195663263679647
8	0.2546683533368183	1.2122633632557183
9	0.20577446947613473	1.1019604637449882
>10	1.0223345072709173	10.899019024568299
>50	0.07436668861279225	3.086826970439312
>100	0.04420381772580734	4.7937144351906635
>500	0.002593616484792359	1.0916546904537523
>1k	0.0011856532501907419	1.4412240573620736
>5k	2.2230998441076413E-4	1.0155942517659133
>10k+	7.410332813692137E-5	0.6959640372453947
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	15784	0.68595636458772	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9478	0.41190410691601687	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7338	0.31890191354185815	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6217	0.2701844094425909	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3813	0.1657090482876949	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3652	0.1587121543001998	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3529	0.15336670112962897	No Hit
GTGGTATCAACGCAGAGTACATGGG	3241	0.14085051809609733	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2568	0.11160263204899043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.3458968866429295E-5	0.0
10	0.0	0.0	0.0	4.3458968866429295E-5	0.0
11	0.0	0.0	0.0	4.3458968866429295E-5	0.0
12	0.0	0.0	0.0	4.3458968866429295E-5	0.0
13	0.0	0.0	0.0	4.3458968866429295E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGG	90	2.2610038E-9	14.790727	14
TATAACG	145	0.0	14.403765	2
CGACGGT	110	1.8371793E-10	13.8299885	15
GGTATCA	3505	0.0	13.379836	1
CTCGACG	95	7.5157004E-8	12.983592	13
CGCATAT	215	0.0	12.371851	8
GTTATCA	545	0.0	12.367267	1
GCTCGAG	70	1.1042785E-4	12.19887	11
ATAACGC	165	0.0	12.082497	3
GTGGTAT	1080	0.0	11.866478	1
TGGTATC	1085	0.0	11.724556	2
ATCACGC	130	2.6466296E-9	11.684173	3
CGAGAGC	115	6.9934686E-8	11.575351	14
ACGGTCA	145	1.200533E-9	11.147447	17
TACGATC	60	0.005856022	11.088684	4
TAACGCA	180	3.6379788E-12	11.088683	4
TACACCG	60	0.005856974	11.088443	5
TATCACG	120	1.2884811E-7	11.075622	2
GTATCAA	8780	0.0	11.050153	1
ATCCGAC	70	0.0014810087	10.866656	16
>>END_MODULE