FastQCFastQC Report
Thu 26 May 2016
SRR1364288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301021
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT355411.5445752124817635No Hit
GGTATCAACGCAGAGTACTTTTTTT261071.1345833001958694No Hit
TATCAACGCAGAGTACTTTTTTTTT257231.117895056151161No Hit
ACGCAGAGTACTTTTTTTTTTTTTT123340.536022921998539No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71900.3124699861496266No Hit
GAGTACTTTTTTTTTTTTTTTTTTT67290.2924354015022027No Hit
GTGGTATCAACGCAGAGTACATGGG54450.23663408547770748No Hit
GTGGTATCAACGCAGAGTACTTTTT46120.20043276441197191No Hit
ATCAACGCAGAGTACTTTTTTTTTT38110.16562213034996204No Hit
GGTATCAACGCAGAGTACATGGGAT32580.14158932056682663No Hit
GCAGAGTACTTTTTTTTTTTTTTTT29320.12742169671637069No Hit
GGTATCAACGCAGAGTACATGGGAG23430.10182436405404384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA250.006033458318.99971810
TATAACG2500.016.3408242
ATAACGC2550.015.2759433
TAACGCA2750.014.5113994
GGGTCGC400.00527912214.2504076
GTCTACG551.9570682E-413.8212821
GGTATCA64850.013.670761
GTGGTAT18100.013.5444761
CGACGGT1351.8189894E-1213.36813815
TGGTATC17950.013.3377192
TCGACGG1403.6379788E-1212.89070414
GTTCACG604.0856155E-412.6695081
CTCGACG1457.2759576E-1212.44619713
GCGGTAT1551.8189894E-1212.2608141
AACCGTC550.003068557712.0912557
ACCGTCC802.867562E-511.8750818
GTCGGTT802.875867E-511.87172512
TTGGGAC1052.7185524E-711.7630093
CCGTGAA658.0226E-411.6928972
TTTAGGC2700.011.6122023
Produced by FastQC (version 0.11.5)