##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364288_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2301021
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.657185223429078	31.0	30.0	34.0	26.0	34.0
2	29.68151790009739	31.0	30.0	34.0	26.0	34.0
3	29.708249077257445	31.0	30.0	34.0	26.0	34.0
4	33.036294323259106	35.0	33.0	37.0	27.0	37.0
5	32.639423542853365	35.0	33.0	37.0	25.0	37.0
6	32.51750244782642	35.0	33.0	37.0	25.0	37.0
7	32.23415083999668	35.0	32.0	37.0	22.0	37.0
8	32.12131266946282	35.0	32.0	37.0	19.0	37.0
9	32.78269037961844	37.0	32.0	39.0	16.0	39.0
10	32.96591860743557	37.0	32.0	39.0	17.0	39.0
11	32.89086801033107	37.0	32.0	39.0	17.0	39.0
12	32.8310476088658	37.0	32.0	39.0	17.0	39.0
13	32.87424582391903	37.0	32.0	39.0	17.0	39.0
14	33.78130621146004	38.0	32.0	41.0	17.0	41.0
15	33.81765398925086	38.0	33.0	41.0	17.0	41.0
16	33.78143615377695	38.0	33.0	41.0	17.0	41.0
17	33.80319388653993	38.0	33.0	41.0	17.0	41.0
18	33.7850149998631	38.0	33.0	41.0	17.0	41.0
19	33.730099812213794	39.0	33.0	41.0	16.0	41.0
20	33.64305106298465	39.0	33.0	41.0	11.0	41.0
21	33.604881485218954	39.0	33.0	41.0	10.0	41.0
22	33.55211795111822	39.0	33.0	41.0	10.0	41.0
23	33.29490126339569	39.0	32.0	41.0	2.0	41.0
24	33.20264656428603	38.0	32.0	41.0	2.0	41.0
25	33.044670170328736	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.3473811860196676
1101	2	0.543360045600803
1101	3	-1.2162956212358154
1101	4	-0.3170825735634253
1101	5	0.6178838729316638
1101	6	0.7642654971270417
1101	7	0.9710177339636985
1101	8	1.0811114110790854
1101	9	1.7569496112677925
1101	10	1.3769581310039598
1101	11	1.3868937744132452
1101	12	1.4533912695223279
1101	13	1.2679194608158468
1101	14	1.442525829840406
1101	15	1.388223082180538
1101	16	1.4000511199664984
1101	17	1.3209695252994607
1101	18	1.4147271302927678
1101	19	1.4210270813293349
1101	20	1.691799331017478
1101	21	1.6060855786901485
1101	22	1.7525090951655287
1101	23	1.9538272919505744
1101	24	2.015920753164977
1101	25	2.1525681195845294
1102	1	0.6126093512166833
1102	2	0.6179106207996021
1102	3	0.7319305935571876
1102	4	0.728938301572633
1102	5	0.7357452038167978
1102	6	0.872133382812919
1102	7	0.9618308299343994
1102	8	0.8973110298181979
1102	9	1.6429580583412857
1102	10	1.0833620711007015
1102	11	1.0709951152408337
1102	12	1.2625435353385441
1102	13	1.0092699903114521
1102	14	1.0206894833700773
1102	15	1.0374009257513208
1102	16	1.1053739789821577
1102	17	1.0702250242160147
1102	18	1.1078624698895752
1102	19	1.0779737469001986
1102	20	1.3581462623885159
1102	21	1.575167525726556
1102	22	1.4598334335215313
1102	23	1.8214482200061255
1102	24	1.7259160826188875
1102	25	1.5681252475795446
1103	1	-2.145064601122858
1103	2	-2.1068576592388837
1103	3	-2.0796073633012355
1103	4	-1.9388448647980567
1103	5	-4.8509862978356395
1103	6	-8.553280437655381
1103	7	-9.637463111253368
1103	8	-10.049666471952566
1103	9	-11.087294224979935
1103	10	-9.011887120197212
1103	11	-10.894920574819114
1103	12	-10.341191285074562
1103	13	-11.023405969942747
1103	14	-11.168567132088985
1103	15	-11.319159765989326
1103	16	-5.83168855590846
1103	17	-3.860746340442109
1103	18	-3.2766249268515537
1103	19	-2.9504649350219943
1103	20	-7.974996029846853
1103	21	-4.741674135246885
1103	22	-10.264130689092479
1103	23	-11.375306923808491
1103	24	-12.441259751541576
1103	25	-12.968737256043791
1104	1	0.12925281434515057
1104	2	0.357379302557046
1104	3	0.4038339635385775
1104	4	0.45619677280872395
1104	5	0.3784348291516082
1104	6	0.46113128088194344
1104	7	0.3137083252092907
1104	8	0.638830381631287
1104	9	1.0455407684927636
1104	10	0.7255048614587949
1104	11	0.642868210944421
1104	12	0.5994629548876063
1104	13	0.5720734039929667
1104	14	0.6567625610584429
1104	15	0.6149542600523077
1104	16	0.2501500558931369
1104	17	0.13490113378151847
1104	18	0.30128935789761613
1104	19	0.5224641628199223
1104	20	0.8570984083385369
1104	21	0.6858698718586282
1104	22	0.4372995542555387
1104	23	0.8486410088162799
1104	24	1.0410966727308377
1104	25	1.1747758810135807
1105	1	0.3351207974688819
1105	2	0.34382269402699706
1105	3	0.09543828407930732
1105	4	0.24005902271732538
1105	5	0.48249865756615407
1105	6	0.37098806201379375
1105	7	0.6055119303327032
1105	8	0.31827189096907205
1105	9	0.5937755228637656
1105	10	0.16349493864771603
1105	11	0.7432898786425568
1105	12	0.9670069675487909
1105	13	0.7338183308781439
1105	14	0.5145454523734756
1105	15	0.30660278640574745
1105	16	0.5467591116330794
1105	17	0.6984811992698425
1105	18	0.5119313298432502
1105	19	0.6183378090666878
1105	20	0.8203314382845903
1105	21	0.93449124787805
1105	22	0.6690784359838844
1105	23	1.0479403267723626
1105	24	0.9503732824397062
1105	25	1.080613358144042
1106	1	-0.2595607470283028
1106	2	0.038922596130831266
1106	3	-0.15691696101510288
1106	4	-1.3484267440912383
1106	5	-0.5403332840900106
1106	6	-0.2852454337745556
1106	7	-0.07181391742939525
1106	8	0.14049220535980567
1106	9	0.5164506516220442
1106	10	0.09048881016855859
1106	11	0.22319553049839413
1106	12	0.3616049381766828
1106	13	0.23797309959336133
1106	14	-0.3072241978613377
1106	15	0.30718625733351246
1106	16	0.0509691351265289
1106	17	0.006970009566927615
1106	18	0.2092386562687878
1106	19	-1.1829394795919228
1106	20	-0.07191376923819348
1106	21	-0.27152501281383223
1106	22	0.22527807906568142
1106	23	0.20253286143680072
1106	24	0.3999475602418485
1106	25	0.6083368531430366
1107	1	0.1727042770668774
1107	2	-0.051787271892465014
1107	3	-0.10586806943197402
1107	4	-0.028588815210426333
1107	5	0.28266660349481043
1107	6	0.06948565076132951
1107	7	-0.0952400807895799
1107	8	-0.2138953525670253
1107	9	0.6424099251172208
1107	10	0.46544270867214976
1107	11	0.5274008244479802
1107	12	0.46475906969213554
1107	13	0.27248713586835294
1107	14	0.33029853883300575
1107	15	0.041410526578488316
1107	16	0.01658764568663429
1107	17	-0.025464184875254148
1107	18	0.2477023627550139
1107	19	0.4132863016210848
1107	20	0.29016971884048104
1107	21	0.16886691996382552
1107	22	0.32614509319939344
1107	23	0.5377914791553025
1107	24	0.6964699693538563
1107	25	0.40595136442851754
1108	1	-0.08080032843665208
1108	2	-0.0032157733949986778
1108	3	0.048334572723288005
1108	4	-0.06449845678704946
1108	5	0.308183488010215
1108	6	0.28817845344929083
1108	7	0.10429823255947213
1108	8	0.26783909282335827
1108	9	0.5417496485247852
1108	10	0.3937073367617856
1108	11	0.40030151374387657
1108	12	0.5606189239313082
1108	13	0.046004572799439813
1108	14	0.2055190243644347
1108	15	-0.13578641835300687
1108	16	-0.6546210083595483
1108	17	-0.6567214724792905
1108	18	-0.6241634212119891
1108	19	-0.5534472609530994
1108	20	0.08653985832543754
1108	21	-0.08185113301563263
1108	22	0.11425871601486648
1108	23	-0.3519841107145183
1108	24	0.011154611673447334
1108	25	-0.14665049595365787
1109	1	0.22323466689654836
1109	2	0.15936379738439754
1109	3	0.11936368488378335
1109	4	0.2219980398931014
1109	5	0.21262938027059164
1109	6	0.3331340749416256
1109	7	0.41898149333068346
1109	8	0.34890773712504597
1109	9	1.1679503197162902
1109	10	0.6017893506329699
1109	11	0.5956952990742366
1109	12	0.6499607822893694
1109	13	0.5492585282581501
1109	14	0.09445131303221643
1109	15	-0.07326825179804786
1109	16	0.3520390663638864
1109	17	0.31711556547148234
1109	18	0.24478809677616908
1109	19	0.4378059356384014
1109	20	0.6940707565954654
1109	21	0.7663078918243826
1109	22	0.4800617548408397
1109	23	0.828839814526134
1109	24	0.6695804384541972
1109	25	0.7260661588784743
1110	1	0.13719947168477375
1110	2	0.15907675119228415
1110	3	-0.01523209359137212
1110	4	0.1614891400281806
1110	5	0.20451055853261124
1110	6	0.3295111537827893
1110	7	0.4035366584652067
1110	8	0.306272176430916
1110	9	-3.128756492690801
1110	10	-1.0697816126008277
1110	11	-0.23332396982529247
1110	12	0.1338541422909998
1110	13	-0.14192253182169168
1110	14	0.17998315924604924
1110	15	0.32234430070327136
1110	16	-0.07299639960348969
1110	17	0.053072286809666025
1110	18	0.10692620626852545
1110	19	0.0478853597962825
1110	20	0.34680678345683447
1110	21	0.13196522081658202
1110	22	0.460056988335495
1110	23	0.5847121566526141
1110	24	0.7075465585565581
1110	25	0.8251357098842362
1111	1	0.3721458336731587
1111	2	0.37058001177304334
1111	3	0.36855570030716933
1111	4	0.260585727332149
1111	5	0.39443710488564676
1111	6	0.5266985236202544
1111	7	0.490472124947118
1111	8	0.590678976961378
1111	9	1.304532095659475
1111	10	0.8976704625471754
1111	11	0.9490599980973542
1111	12	1.1095071568774486
1111	13	0.7725392882475077
1111	14	0.68619723291296
1111	15	0.7839961711849597
1111	16	0.7529302987469819
1111	17	0.8626336328000264
1111	18	0.7410819164530054
1111	19	0.8285169680764923
1111	20	1.1617216207046894
1111	21	1.098415173302648
1111	22	1.0690574166383584
1111	23	1.4047298072081418
1111	24	1.2076287432193098
1111	25	1.3536721984016538
1112	1	0.002641457543131054
1112	2	0.005624580812181534
1112	3	0.008937637861929915
1112	4	0.07541115916513519
1112	5	0.058778742125447536
1112	6	0.016405852913720764
1112	7	0.13609778013745455
1112	8	-0.3603420517584226
1112	9	0.4563101746809224
1112	10	0.33988099206681
1112	11	0.27710820254659296
1112	12	-0.24862902672041542
1112	13	-0.011556070590465595
1112	14	-0.8183841974052228
1112	15	-0.18421491555741198
1112	16	-0.21315783960814372
1112	17	-0.25109511796161854
1112	18	-0.37607006663365183
1112	19	-0.008566644014535996
1112	20	0.07865007084701148
1112	21	-0.22291636076315058
1112	22	0.07590768585536978
1112	23	0.1385078651581324
1112	24	0.06702950635691707
1112	25	0.15763090889927156
1113	1	-1.903189315879363
1113	2	-1.9592879571614255
1113	3	-1.8823499941025865
1113	4	-1.6723926732413545
1113	5	-1.676438277530501
1113	6	-1.8077237030156006
1113	7	-2.1398229907814468
1113	8	-1.9662909062487977
1113	9	-11.647270257526394
1113	10	-4.316054451848718
1113	11	-2.8310194984303223
1113	12	-13.364797546851793
1113	13	-4.670451617301989
1113	14	-3.63345173272009
1113	15	-2.964821311572752
1113	16	-2.5866829420364574
1113	17	-2.98286555612399
1113	18	-3.23448308768549
1113	19	-3.954092718975186
1113	20	-15.108911376576948
1113	21	-17.740894819681948
1113	22	-11.970031927877567
1113	23	-18.761805444975543
1113	24	-19.953931795952293
1113	25	-16.023724269006564
1114	1	-0.09508653843090897
1114	2	-0.032084913500408874
1114	3	-0.083146907330228
1114	4	-0.5403544765889521
1114	5	-0.10053536428130627
1114	6	-0.03515502125277692
1114	7	-0.09589662496012608
1114	8	0.11173524078244412
1114	9	0.7667495521370142
1114	10	0.15935095646961628
1114	11	7.244632868008694E-4
1114	12	-0.1768915726970448
1114	13	-0.10367214261373903
1114	14	-0.215053750331073
1114	15	0.005676516693199574
1114	16	-0.0035834518281205874
1114	17	-0.13892798135275086
1114	18	-0.16008907154029117
1114	19	-0.40805540610537605
1114	20	-0.5743798352385525
1114	21	-0.7174228474456541
1114	22	-0.9344249740174817
1114	23	-0.42460574244383054
1114	24	-0.12878408570364996
1114	25	-0.4460186548315548
1115	1	-0.38389687144004725
1115	2	-0.3414484314320845
1115	3	-0.351073809688458
1115	4	-0.23154242093310984
1115	5	-0.5157255193553993
1115	6	-0.5324561363076299
1115	7	-0.8502857493172726
1115	8	-0.5921866515449565
1115	9	-0.1823605931754102
1115	10	-0.45409997142685654
1115	11	-0.3312830302263521
1115	12	-0.4297257212968546
1115	13	-0.5164344424906417
1115	14	-0.6722009038368384
1115	15	-0.7454688639219995
1115	16	-0.5550939617674331
1115	17	-0.5982775386684338
1115	18	-0.6502484522487961
1115	19	-0.4009942054923954
1115	20	-0.866608473522227
1115	21	-0.38340739386919154
1115	22	-0.9205951356232802
1115	23	-0.5249061289626837
1115	24	-0.2676737681265564
1115	25	-0.26390984815916596
1116	1	-0.8203997960177283
1116	2	-0.7518586962500677
1116	3	-0.7564006970898447
1116	4	-0.5416790669444183
1116	5	-1.6998175735870298
1116	6	-1.3303432678865335
1116	7	-2.624763202055078
1116	8	-1.84990713442566
1116	9	-0.5128200655280857
1116	10	-1.851162084976771
1116	11	-1.4095203011493318
1116	12	-2.403264940690736
1116	13	-2.0481766260673098
1116	14	-1.6663514691732644
1116	15	-2.8375135992632217
1116	16	-3.001800439297817
1116	17	-3.742681668883783
1116	18	-2.800543842694303
1116	19	-2.4940579764240347
1116	20	-2.8183384180583104
1116	21	-2.1093183114173826
1116	22	-1.9295678019521745
1116	23	-2.0727013699812886
1116	24	-2.63005522511423
1116	25	-2.2114848171705432
1201	1	0.6084974100344809
1201	2	0.7216086474025367
1201	3	0.2832948413215526
1201	4	0.5643263761342041
1201	5	0.905387840722419
1201	6	0.9459846205473497
1201	7	1.1014881961934009
1201	8	1.0448973899816991
1201	9	1.5838671856625908
1201	10	1.384481021316816
1201	11	1.3659467881935115
1201	12	1.3873553535923904
1201	13	1.2363896240533236
1201	14	1.3303737615718774
1201	15	1.2611616228936526
1201	16	1.1519634210393903
1201	17	1.26110407180402
1201	18	1.263732039089895
1201	19	1.2627370711513137
1201	20	1.4105125995145542
1201	21	1.3653639834641922
1201	22	1.2936977326415402
1201	23	1.7288430914373691
1201	24	1.596447675777192
1201	25	1.7269522672621207
1202	1	0.8360176822281069
1202	2	0.9657123529441165
1202	3	0.8615976317293814
1202	4	0.9152105919041986
1202	5	1.0877089588089035
1202	6	1.0558225487830128
1202	7	1.1657293160493793
1202	8	1.120104054801942
1202	9	1.8776305530008912
1202	10	1.5812607429684462
1202	11	1.7271899698892952
1202	12	1.705363610568412
1202	13	1.4527460049859187
1202	14	1.6536652426344958
1202	15	1.6546167240238177
1202	16	1.5531600382372872
1202	17	1.5519167675056948
1202	18	1.541688048045053
1202	19	1.64196557377862
1202	20	1.9960212507789663
1202	21	1.9891727035848277
1202	22	1.9758604141103717
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2211	3	-0.03849382909135457
2211	4	-0.03143814891426899
2211	5	-0.7606749379419888
2211	6	0.05444206940541818
2211	7	0.30743804996692603
2211	8	0.05443601267797504
2211	9	0.7280864981905708
2211	10	-0.03366202344960101
2211	11	0.3848452642182565
2211	12	0.33961161119233196
2211	13	0.3355895975291432
2211	14	0.39461133038015817
2211	15	0.31919834394384594
2211	16	0.26512876194525603
2211	17	0.15400665907617395
2211	18	0.0678500967674367
2211	19	-0.033918074822764765
2211	20	0.25317741008623074
2211	21	0.5659303702231355
2211	22	0.40439749001103564
2211	23	0.7497865874732312
2211	24	0.7750525369299908
2211	25	0.9588825224169355
2212	1	0.12072195428786969
2212	2	0.1930337527727417
2212	3	0.21183232517216766
2212	4	0.35420494292889515
2212	5	0.012534122254010072
2212	6	0.16233185959145402
2212	7	0.2121774325841983
2212	8	0.19426534825514352
2212	9	0.7733865132438495
2212	10	0.2962312968118326
2212	11	0.5034879441641493
2212	12	0.22885825274079963
2212	13	0.15855620628022393
2212	14	0.19440816632539537
2212	15	0.613681181281919
2212	16	0.43216716543805234
2212	17	0.09078084177439649
2212	18	0.038364703681246226
2212	19	0.3490970352453431
2212	20	0.6191435848839646
2212	21	0.7628685453803783
2212	22	0.3999824144606947
2212	23	0.8609801025449784
2212	24	0.8942599903406645
2212	25	1.0498121530493023
2213	1	0.004286912997336856
2213	2	-0.01897666299489842
2213	3	-0.15217575704400943
2213	4	-0.07835749541388992
2213	5	0.06832659567654531
2213	6	0.17352438523484892
2213	7	0.0889794892995539
2213	8	0.09519934239722971
2213	9	0.725365306985644
2213	10	0.18373822044591748
2213	11	0.35981297751230557
2213	12	0.2827414691962744
2213	13	0.013795034784948257
2213	14	0.08635755032680237
2213	15	0.13213751404460794
2213	16	0.0191596917369381
2213	17	-0.1155295249405981
2213	18	-0.19817681681981725
2213	19	-0.03171772490468072
2213	20	0.1587897885475087
2213	21	0.019339890002015636
2213	22	0.07015182090177774
2213	23	-0.20635039542136013
2213	24	-0.10338337941253428
2213	25	0.2654134358471154
2214	1	-0.76790448106253
2214	2	-0.5239685466402264
2214	3	-0.5829800357084132
2214	4	-0.43336717427533955
2214	5	-0.6980737576236002
2214	6	-0.556664873399086
2214	7	-0.42615458246291027
2214	8	-0.42928915364658593
2214	9	-10.511659376281209
2214	10	-3.5300729289080266
2214	11	-1.5049894955887062
2214	12	-0.7543973638715791
2214	13	-0.5612535765371476
2214	14	-0.7478962875804811
2214	15	-0.7127272640143261
2214	16	-0.9588171006970541
2214	17	-0.8021194189939536
2214	18	-1.220231491536282
2214	19	-1.3427694221776676
2214	20	-0.9978379528923398
2214	21	-1.5213579862908517
2214	22	-1.3252443179703555
2214	23	-1.858775102125815
2214	24	-1.9839102046157677
2214	25	-10.050406077258412
2215	1	-0.6734838939869512
2215	2	-0.415214269906663
2215	3	-0.2907294545864687
2215	4	-0.4115851544842428
2215	5	-0.4410881468842689
2215	6	-0.4216364009371709
2215	7	-0.5368450480418403
2215	8	-0.6156151113947494
2215	9	0.23943291515438148
2215	10	0.16066980692806965
2215	11	0.13413182346036479
2215	12	0.1562239311618825
2215	13	-0.14472039198890485
2215	14	-0.16816822807538045
2215	15	-0.6062821162289538
2215	16	-0.3774892105299372
2215	17	-0.3221469029062902
2215	18	-0.351107636885466
2215	19	-0.4032819209070766
2215	20	-0.11100230568278846
2215	21	-0.09313618033922211
2215	22	-0.27678137294152805
2215	23	0.062475474528106645
2215	24	0.2653353980141091
2215	25	0.2906903024161167
2216	1	-0.6464211245031528
2216	2	-0.5981846808821629
2216	3	-0.6248251117688355
2216	4	-0.5155167155919784
2216	5	-1.2776605380464403
2216	6	-1.71485673899533
2216	7	-0.9673447957983186
2216	8	-1.8143787770865458
2216	9	-0.46285793076589243
2216	10	-1.1409685618080303
2216	11	-0.5794987943466339
2216	12	-1.736200004933881
2216	13	-1.3252637264436231
2216	14	-1.37960859724312
2216	15	-1.112816983268381
2216	16	-1.978977051682822
2216	17	-2.401912061323131
2216	18	-1.9985975205424076
2216	19	-3.4153181250145117
2216	20	-2.2990662895915897
2216	21	-2.473766060651105
2216	22	-2.3727393649139366
2216	23	-1.9605770458744232
2216	24	-2.114008845226678
2216	25	-2.169818784762466
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81040.0
3	2.0
4	1115.0
5	11414.0
6	16157.0
7	13253.0
8	10387.0
9	8267.0
10	7095.0
11	6375.0
12	5727.0
13	5243.0
14	5017.0
15	5205.0
16	5407.0
17	5711.0
18	6415.0
19	8443.0
20	11972.0
21	15567.0
22	18903.0
23	22151.0
24	25436.0
25	28567.0
26	31550.0
27	34446.0
28	38407.0
29	44034.0
30	53695.0
31	66839.0
32	82424.0
33	102196.0
34	128158.0
35	158654.0
36	208839.0
37	304070.0
38	722840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.913342667872826	19.32458332554489	13.891829177057899	22.870244829524385
2	16.115906127770536	21.12477183833116	39.089787049109084	23.669534984789223
3	18.866953670071247	26.5798442254689	29.88633726463678	24.66686483982307
4	11.881814641744548	17.431966580440587	36.29339988039608	34.39281889741878
5	12.263073532793767	39.51424178096276	33.568685688748	14.653998997495476
6	30.363187496196876	38.07288089918895	17.100280779227553	14.463650825386615
7	27.392719333058324	34.32985688956158	20.246630017156907	18.030793760223183
8	27.0884439442331	35.32893315440489	18.570998204194908	19.011624697167097
9	26.819707167013227	15.788670557020875	19.409238859430342	37.982383416535555
10	15.669284046993722	31.916011959287644	30.197418675496284	22.21728531822235
11	33.8025927816361	24.11848641654761	21.548486616540828	20.530434185275457
12	23.440512512930173	28.445380342660442	28.669648212376458	19.444458932032926
13	28.805407174362855	22.411115790352042	26.291915966627595	22.491561068657504
14	22.036634207718436	22.326656829743726	28.57976708658303	27.05694187595481
15	24.069949983571245	29.39530015941976	24.836755555787356	21.697994301221637
16	21.95681039297457	29.76410225205745	26.278031193701278	22.0010561612667
17	21.09387869349656	29.169794246875064	27.535600084667415	22.200726974960958
18	20.509578392513063	26.56948241081748	31.268030254098083	21.652908942571372
19	21.887087863486226	26.36765230680826	31.610625562080898	20.13463426762462
20	22.20021880744293	25.582519397753593	31.60089765818661	20.616364136616866
21	23.09302834014784	24.96699959012991	31.341196638109054	20.5987754316132
22	22.20477382839444	25.349005303915717	31.471826832345755	20.974394035344083
23	21.563188616182995	25.436824422703307	31.67267179819281	21.327315162920886
24	21.437847422931323	25.72948493395976	31.760073363553786	21.072594279555123
25	21.431809579332516	25.358648504017857	31.786868770366635	21.422673146282996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1601.0
1	1601.0
2	2061.0
3	2521.0
4	2521.0
5	2521.0
6	6088.0
7	9655.0
8	9655.0
9	9655.0
10	11325.0
11	12995.0
12	12995.0
13	12995.0
14	13869.5
15	14744.0
16	14744.0
17	14744.0
18	25201.0
19	35658.0
20	35658.0
21	35658.0
22	53926.0
23	72194.0
24	72194.0
25	72194.0
26	102898.0
27	133602.0
28	133602.0
29	133602.0
30	155262.0
31	176922.0
32	176922.0
33	176922.0
34	192817.0
35	208712.0
36	208712.0
37	208712.0
38	215184.5
39	221657.0
40	221657.0
41	221657.0
42	237890.5
43	254124.0
44	254124.0
45	254124.0
46	270265.0
47	286406.0
48	286406.0
49	286406.0
50	280355.5
51	274305.0
52	274305.0
53	274305.0
54	247488.0
55	220671.0
56	220671.0
57	220671.0
58	191722.5
59	162774.0
60	162774.0
61	162774.0
62	133543.5
63	104313.0
64	104313.0
65	104313.0
66	79614.0
67	54915.0
68	54915.0
69	54915.0
70	41471.5
71	28028.0
72	28028.0
73	28028.0
74	20157.5
75	12287.0
76	12287.0
77	12287.0
78	9884.0
79	7481.0
80	7481.0
81	7481.0
82	5417.5
83	3354.0
84	3354.0
85	3354.0
86	2425.5
87	1497.0
88	1497.0
89	1497.0
90	1027.0
91	557.0
92	557.0
93	557.0
94	301.5
95	46.0
96	46.0
97	46.0
98	24.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02624921719532329
2	9.126383461950152E-4
3	1.3037690659928788E-4
4	0.004041684104577925
5	0.03398491365354771
6	0.012211970251466633
7	0.021294894744550354
8	0.02868291945184333
9	0.0348540930308763
10	0.0384177284779235
11	0.040981807641042825
12	0.008735252742152288
13	0.003389799571581485
14	0.007214188831827263
15	0.007692237489357985
16	0.010256316652477313
17	0.01103857809207304
18	0.016253654356044554
19	0.018165848986167445
20	0.01599290054284598
21	0.012690018908997354
22	0.01164700365620305
23	0.009169842430816581
24	0.006562304298830823
25	0.10999465020093255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2301021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.47114558804148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3579930960644	31.15598004829493
2	12.113278922434532	10.289296653265424
3	5.079446639556241	6.471897532058433
4	2.7314411262456284	4.640297349517683
5	1.6655280136855724	3.5368441375100743
6	1.0794748340013252	2.7507919700098316
7	0.7434785686559122	2.2103470571681756
8	0.5324073398416901	1.8089559714046637
9	0.38647800973513646	1.4772747436233742
>10	2.0589412886751366	15.636592801070654
>50	0.15140180422608093	4.4784010671207
>100	0.09167871579908407	7.14136177514812
>500	0.005498658051543389	1.5318120632221752
>1k	0.00224019402099915	1.7207586161362773
>5k	3.054810028635204E-4	0.8374367247948649
>10k+	4.0730800381802727E-4	4.311951489654617
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	35541	1.5445752124817635	No Hit
GGTATCAACGCAGAGTACTTTTTTT	26107	1.1345833001958694	No Hit
TATCAACGCAGAGTACTTTTTTTTT	25723	1.117895056151161	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	12334	0.536022921998539	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7190	0.3124699861496266	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	6729	0.2924354015022027	No Hit
GTGGTATCAACGCAGAGTACATGGG	5445	0.23663408547770748	No Hit
GTGGTATCAACGCAGAGTACTTTTT	4612	0.20043276441197191	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	3811	0.16562213034996204	No Hit
GGTATCAACGCAGAGTACATGGGAT	3258	0.14158932056682663	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2932	0.12742169671637069	No Hit
GGTATCAACGCAGAGTACATGGGAG	2343	0.10182436405404384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	4.3458968866429295E-5	0.0	0.0	0.0	0.0
11	4.3458968866429295E-5	0.0	0.0	4.3458968866429295E-5	0.0
12	4.3458968866429295E-5	0.0	0.0	4.3458968866429295E-5	0.0
13	4.3458968866429295E-5	0.0	0.0	4.3458968866429295E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	25	0.0060334583	18.999718	10
TATAACG	250	0.0	16.340824	2
ATAACGC	255	0.0	15.275943	3
TAACGCA	275	0.0	14.511399	4
GGGTCGC	40	0.005279122	14.250407	6
GTCTACG	55	1.9570682E-4	13.821282	1
GGTATCA	6485	0.0	13.67076	1
GTGGTAT	1810	0.0	13.544476	1
CGACGGT	135	1.8189894E-12	13.368138	15
TGGTATC	1795	0.0	13.337719	2
TCGACGG	140	3.6379788E-12	12.890704	14
GTTCACG	60	4.0856155E-4	12.669508	1
CTCGACG	145	7.2759576E-12	12.446197	13
GCGGTAT	155	1.8189894E-12	12.260814	1
AACCGTC	55	0.0030685577	12.091255	7
ACCGTCC	80	2.867562E-5	11.875081	8
GTCGGTT	80	2.875867E-5	11.871725	12
TTGGGAC	105	2.7185524E-7	11.763009	3
CCGTGAA	65	8.0226E-4	11.692897	2
TTTAGGC	270	0.0	11.612202	3
>>END_MODULE