FastQCFastQC Report
Thu 26 May 2016
SRR1364287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123630
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39140.3483353061061025No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN29220.2600500164644945No Hit
TATCAACGCAGAGTACTTTTTTTTT25110.22347213940532026No Hit
GGTATCAACGCAGAGTACTTTTTTT17750.15797015031638528No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15610.13892473501063518No Hit
GTGGTATCAACGCAGAGTACATGGG11990.10670772407287096No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11930.10617374046616769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA402.7590632E-416.62593810
TTGCGCG2300.014.05368618
TTTGCGC2450.013.58129417
TACACCG500.001494422813.3055065
TCTCGAC500.001511618313.28532112
TATAACG655.4904765E-513.1434342
TGCGCGC2600.012.43155219
CGAGCCT701.0861707E-412.219344
CGTGACA550.003049851212.1002414
TAACGCA1103.787318E-812.0959144
TATCACG550.003085772512.0813382
CTCGACG550.00309312.07756413
AGGACCG951.0337171E-612.0049685
ATTTGCG2800.011.88363216
GGTATCA13350.011.7322781
GTGTTAC658.0738077E-411.6830521
ACGGCCC907.3951633E-611.61955215
CGAGGTT907.4209656E-611.6159174
AACTGGC1803.6379788E-1211.09139117
TAAGGTA600.00585585211.0879214
Produced by FastQC (version 0.11.5)