##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364287_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1123630 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.61091996475708 30.0 26.0 31.0 10.0 34.0 2 26.46868364141221 31.0 26.0 31.0 10.0 34.0 3 25.84005233039346 30.0 25.0 31.0 10.0 34.0 4 29.701456885273622 35.0 28.0 37.0 10.0 37.0 5 30.29987273390707 35.0 30.0 37.0 10.0 37.0 6 30.44985626941253 35.0 31.0 37.0 10.0 37.0 7 30.123684842875324 35.0 30.0 37.0 8.0 37.0 8 29.651154739549497 35.0 30.0 37.0 2.0 37.0 9 30.722446890880448 37.0 30.0 39.0 2.0 39.0 10 29.900898872404618 35.0 27.0 39.0 2.0 39.0 11 30.680627074748806 35.0 30.0 39.0 2.0 39.0 12 30.12604594038963 35.0 27.0 39.0 2.0 39.0 13 29.769101038598116 35.0 27.0 39.0 2.0 39.0 14 30.02470742148216 36.0 27.0 40.0 2.0 41.0 15 30.8152523517528 36.0 27.0 40.0 2.0 41.0 16 31.16907077952707 37.0 30.0 40.0 2.0 41.0 17 31.339941973781404 37.0 30.0 40.0 2.0 41.0 18 31.169220294936945 37.0 30.0 40.0 2.0 41.0 19 31.15420556588913 37.0 30.0 40.0 2.0 41.0 20 31.04161512241574 37.0 30.0 40.0 2.0 41.0 21 30.938104180201666 37.0 29.0 40.0 2.0 41.0 22 31.018511431699046 37.0 30.0 40.0 2.0 41.0 23 31.02610823847708 37.0 30.0 40.0 2.0 41.0 24 30.89430862472522 37.0 30.0 40.0 2.0 41.0 25 30.687400656799838 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.648555108211518 1101 2 0.6677782970165929 1101 3 1.4806316426071717 1101 4 0.94164188248455 1101 5 0.3324134022857663 1101 6 0.21211815631528452 1101 7 0.590004084170257 1101 8 0.8897507007437717 1101 9 0.8131933495232531 1101 10 1.1155420772312894 1101 11 0.509583081107742 1101 12 0.8904005360449432 1101 13 0.6902689554373609 1101 14 1.354222492989571 1101 15 0.2788685593220066 1101 16 -0.11688775777647464 1101 17 -0.7448699670399499 1101 18 -6.428241942384094 1101 19 -8.0693256557118 1101 20 -9.266237092063545 1101 21 -11.036279324157494 1101 22 -11.191754312783349 1101 23 -12.687579997388216 1101 24 -12.159282651536266 1101 25 -12.86093018134061 1102 1 1.097554362343189 1102 2 1.3364001900548743 1102 3 2.2613012060061344 1102 4 1.5186144596743958 1102 5 0.7232368076904407 1102 6 0.7078077020253488 1102 7 0.996957558477618 1102 8 1.479651975868233 1102 9 1.601119688206417 1102 10 -2.05476954147856 1102 11 0.18557179557749492 1102 12 1.2104661498845495 1102 13 1.6251301013406838 1102 14 2.232927811040181 1102 15 1.9044435974697684 1102 16 1.4321384278767013 1102 17 0.9050280913509745 1102 18 1.180804854191372 1102 19 1.0132174198303332 1102 20 1.3959999297804586 1102 21 1.5283668304507572 1102 22 1.2186836426658303 1102 23 1.2196868419453502 1102 24 1.5481914884029315 1102 25 1.609643447526718 1103 1 -0.6187452243062062 1103 2 -0.01237381067174681 1103 3 -3.1000858149218793 1103 4 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2208 15 1.6122769562232087 2208 16 1.1508813580650994 2208 17 0.6542349989483327 2208 18 0.7769972915043333 2208 19 1.0378181917825913 2208 20 1.0997727633055163 2208 21 1.2576597549008994 2208 22 1.1533131118729898 2208 23 1.1257494029798316 2208 24 1.3590335192588476 2208 25 1.3160666644659216 2209 1 0.9612784163557322 2209 2 0.9995778573082923 2209 3 1.8486008149704247 2209 4 1.4944771600654327 2209 5 0.9324338258954583 2209 6 0.7180635291662973 2209 7 0.9782244669327298 2209 8 1.3196253358219217 2209 9 1.523371215135903 2209 10 1.9046430779881653 2209 11 1.379027558924097 2209 12 1.4516374258896043 2209 13 1.8457238613085494 2209 14 -4.3149129275565805 2209 15 -0.6618759413207371 2209 16 -0.07971078119154384 2209 17 0.3623434716952296 2209 18 0.9166050006820896 2209 19 1.1482443552208395 2209 20 1.2772928285246579 2209 21 0.9779781574912931 2209 22 0.8411120807675943 2209 23 0.7134243636531465 2209 24 0.48491133671414843 2209 25 0.6675421326543685 2210 1 0.38651287316389826 2210 2 0.9407306368440942 2210 3 -2.32577003528791 2210 4 -0.18289473733742057 2210 5 0.061277479164044024 2210 6 0.41272165374197556 2210 7 0.7167754950905625 2210 8 1.2548516801368486 2210 9 1.0297124806848466 2210 10 -2.4106348755922617 2210 11 -0.10102548872673012 2210 12 0.24493771471737702 2210 13 1.2191931316444418 2210 14 2.0415267235486745 2210 15 1.591317274103357 2210 16 1.1735773676640804 2210 17 1.1613564516844157 2210 18 1.1883738118853842 2210 19 1.288335894062996 2210 20 1.2734011331861765 2210 21 1.1219749124939966 2210 22 1.006902674878269 2210 23 1.0172159473729252 2210 24 1.1470550486688573 2210 25 1.3444284311711066 2211 1 0.9990764990740431 2211 2 1.1780010466370143 2211 3 1.8410198194850196 2211 4 1.4905954038081113 2211 5 0.9548487365701455 2211 6 0.9397804969955388 2211 7 1.0561063596513804 2211 8 1.2302320770116388 2211 9 -4.469800885345126 2211 10 0.23324878434612728 2211 11 0.42113781967895747 2211 12 -4.799180489208705 2211 13 -0.18255320067900982 2211 14 -5.3854028805911405 2211 15 -1.2303766861721641 2211 16 -0.06202198125522429 2211 17 0.3701601472697895 2211 18 0.9269928351544934 2211 19 1.2532470844474446 2211 20 1.2691074230639288 2211 21 1.3519760195971173 2211 22 1.257379408513593 2211 23 1.1142863443893525 2211 24 1.2803413036222757 2211 25 1.1715000798210902 2212 1 1.2345042515847808 2212 2 1.3135637621211735 2212 3 2.099657954004279 2212 4 1.4015559771402337 2212 5 1.101239303392223 2212 6 1.013410110191323 2212 7 1.131259838715593 2212 8 1.3861949865645968 2212 9 1.3644089509849593 2212 10 2.0633901229054246 2212 11 1.3082103949114305 2212 12 1.578695947679158 2212 13 1.824315680747418 2212 14 -4.105803466831304 2212 15 -0.6860072585070505 2212 16 -0.009636290803925363 2212 17 0.23943990775392976 2212 18 0.7204020032245779 2212 19 1.2422996208437453 2212 20 1.4574592052586794 2212 21 1.3915001700699108 2212 22 1.4117262919277707 2212 23 1.1831799281297073 2212 24 1.2805679226457265 2212 25 1.1277477618082123 2213 1 0.850029030873209 2213 2 1.03884981130237 2213 3 1.6068850821797689 2213 4 1.1938626425902719 2213 5 0.7013649656395913 2213 6 0.4153479335702457 2213 7 0.7564574004962452 2213 8 -4.835538611724008 2213 9 -1.2194955231768319 2213 10 0.6635304297894145 2213 11 0.3754439216666192 2213 12 1.029204679825373 2213 13 -7.717009512420972 2213 14 -1.838338047887678 2213 15 -0.513188436318849 2213 16 0.05580890051698617 2213 17 0.2650167656232263 2213 18 0.7725532440195764 2213 19 0.9070205222323935 2213 20 1.1550775930644264 2213 21 1.1994988452935509 2213 22 0.7822361618496032 2213 23 0.6665106704888686 2213 24 0.8259310927364218 2213 25 1.13611942466164 2214 1 -1.0416585682721795 2214 2 -0.5716212558841036 2214 3 -6.827919709422442 2214 4 -2.5199204555079966 2214 5 -1.3722467989419407 2214 6 -1.0458819163300994 2214 7 -0.6894296781010354 2214 8 -6.502676348433848 2214 9 -1.973159885342568 2214 10 0.041991930454599924 2214 11 -0.452846048369441 2214 12 -0.27691828802445073 2214 13 -6.719701953389382 2214 14 -1.8373367740845694 2214 15 -0.9675841997165655 2214 16 -0.887797244933104 2214 17 -0.7490999094849933 2214 18 -0.6799119606445032 2214 19 -0.27767921417977703 2214 20 -0.1199358977074283 2214 21 0.19342207030362601 2214 22 -0.2241508509007133 2214 23 -0.4049531403906492 2214 24 -0.19937299597443925 2214 25 -0.19647259468622735 2215 1 0.7590262724590744 2215 2 1.189647977462723 2215 3 -2.2156180609421625 2215 4 0.009509694373793565 2215 5 0.336940519182928 2215 6 0.08716104789908385 2215 7 0.7804724012146309 2215 8 1.1733228810151424 2215 9 1.3335605876627525 2215 10 2.2742280099728163 2215 11 1.2669429576948907 2215 12 1.0304254778001685 2215 13 1.53471271184317 2215 14 1.7327961399536136 2215 15 1.554140440094418 2215 16 1.0769770939151968 2215 17 1.0397742235324223 2215 18 1.258816912689113 2215 19 1.2850493177552664 2215 20 1.6434787972772895 2215 21 1.6299001473160502 2215 22 1.599344283117766 2215 23 1.457436487343653 2215 24 1.2171951729962167 2215 25 1.3241271728862571 2216 1 0.9995610475817287 2216 2 0.7041319023000625 2216 3 1.9845546039114694 2216 4 0.7609863352163195 2216 5 0.6522472746548296 2216 6 0.22722496305034312 2216 7 -0.24884984874123006 2216 8 -5.790301202006525 2216 9 -1.2224024238325804 2216 10 0.36614402293725945 2216 11 -0.22794982326208668 2216 12 0.990775083635139 2216 13 1.4751818872794082 2216 14 2.369728601432019 2216 15 1.814357064836674 2216 16 1.0855821530278078 2216 17 0.9780311507783246 2216 18 1.0911233814660903 2216 19 1.1350074473381326 2216 20 1.3381963217880823 2216 21 1.1517603924483133 2216 22 0.8064659794446669 2216 23 0.6252819506799092 2216 24 0.6761675093654951 2216 25 0.7509689448228727 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 92689.0 3 1.0 4 605.0 5 4317.0 6 6765.0 7 5868.0 8 5186.0 9 4322.0 10 3850.0 11 3614.0 12 3349.0 13 3173.0 14 3275.0 15 3319.0 16 3473.0 17 3844.0 18 4434.0 19 5766.0 20 8116.0 21 9983.0 22 12402.0 23 14463.0 24 16655.0 25 18950.0 26 21376.0 27 23311.0 28 25735.0 29 29892.0 30 34465.0 31 38754.0 32 48793.0 33 61760.0 34 76412.0 35 95168.0 36 118744.0 37 136759.0 38 174042.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.08581683124118 16.71972930021606 11.596878738639003 23.597575129903756 2 15.622988390810857 20.70905066777613 39.3561600662821 24.311800875130906 3 17.194141051924767 27.060132437085244 29.927995371131 25.817731139858992 4 11.155956585215577 15.424213877193843 36.57217864174767 36.84765089584292 5 12.672080612667704 39.56362785580591 32.74612796296561 15.018163568560775 6 31.803070343367594 35.23513982191259 18.586724571404094 14.375065263315726 7 30.098825730392843 32.53742560400445 19.775232158349667 17.58851650725305 8 28.501044288544957 32.76120601938628 20.348453203377424 18.389296488691336 9 29.515638695350255 14.957040670783192 18.58202973681721 36.94529089704935 10 13.984228750139618 30.85061990394281 32.33624483413381 22.82890651178376 11 35.77838432235008 23.169090266447213 21.38004162903036 19.67248378217235 12 23.457403890094824 27.03787272934994 28.81378170341597 20.690941677139264 13 28.25046832732845 21.573847566705965 26.390992407697794 23.78469169826779 14 21.39919385070172 21.021378308228936 28.680689189816384 28.89873865125296 15 24.433230936193077 28.345950017581846 23.547088107354565 23.673730938870513 16 23.60421019561362 28.80804838886742 24.428006208369517 23.159735207149442 17 22.948314975752286 28.195798728802213 25.630021115234204 23.225865180211294 18 22.25112807185389 27.040167925632936 28.13245205694621 22.576251945566963 19 23.569908825440343 26.364137768543706 27.94157545640828 22.12437794960767 20 24.1180689447948 25.987276297856965 27.675840705115597 22.218814052232638 21 24.85805367989476 25.349444084484162 27.32758943822992 22.46491279739115 22 24.344361711527185 25.9406518497099 26.806714664253402 22.908271774509515 23 23.323891221156043 26.188831534155817 27.13893291638108 23.34834432830706 24 23.322234052332323 25.991951756949845 27.369202663470453 23.31661152724738 25 23.167661565893148 25.716322980967366 27.868214598379904 23.247800854759586 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3909.0 1 3909.0 2 2692.0 3 1475.0 4 1475.0 5 1475.0 6 2807.0 7 4139.0 8 4139.0 9 4139.0 10 4580.5 11 5022.0 12 5022.0 13 5022.0 14 5840.5 15 6659.0 16 6659.0 17 6659.0 18 9652.5 19 12646.0 20 12646.0 21 12646.0 22 18365.5 23 24085.0 24 24085.0 25 24085.0 26 33681.5 27 43278.0 28 43278.0 29 43278.0 30 53274.0 31 63270.0 32 63270.0 33 63270.0 34 73786.5 35 84303.0 36 84303.0 37 84303.0 38 95031.0 39 105759.0 40 105759.0 41 105759.0 42 117065.0 43 128371.0 44 128371.0 45 128371.0 46 137825.5 47 147280.0 48 147280.0 49 147280.0 50 145563.5 51 143847.0 52 143847.0 53 143847.0 54 134359.0 55 124871.0 56 124871.0 57 124871.0 58 109899.0 59 94927.0 60 94927.0 61 94927.0 62 78489.0 63 62051.0 64 62051.0 65 62051.0 66 48088.0 67 34125.0 68 34125.0 69 34125.0 70 25732.5 71 17340.0 72 17340.0 73 17340.0 74 12709.5 75 8079.0 76 8079.0 77 8079.0 78 6473.0 79 4867.0 80 4867.0 81 4867.0 82 3410.5 83 1954.0 84 1954.0 85 1954.0 86 1454.5 87 955.0 88 955.0 89 955.0 90 653.5 91 352.0 92 352.0 93 352.0 94 199.0 95 46.0 96 46.0 97 46.0 98 33.0 99 20.0 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31772024598844817 2 0.3176312487206643 3 0.3294678853359202 4 0.3324047951727882 5 0.3387236011854436 6 0.28203234160711266 7 0.29137705472441994 8 0.2892411202976069 9 0.2908430711177167 10 0.40093269136637505 11 0.28995309843987793 12 0.28888513122647136 13 0.2806083853225706 14 0.28852914215533587 15 0.28087537712592225 16 0.2854142377829001 17 0.27695949734343156 18 0.2789174372346769 19 0.2796294153769479 20 0.48076324056851455 21 0.27909543177024465 22 0.2809643743937061 23 0.2774934809501349 24 0.27918442903802854 25 0.27420058204213127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1123630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.47958960111878 #Duplication Level Percentage of deduplicated Percentage of total 1 85.80811516007984 55.328720499674866 2 7.7194093400878865 9.9548869242382 3 2.6108501484886677 5.0503963825370795 4 1.2038012842305685 3.104824510739472 5 0.6514659563635341 2.100312875271052 6 0.4263341570444125 1.6493910887497587 7 0.2832364254257933 1.2784077930080122 8 0.2027602318038604 1.0459117227312502 9 0.15424198525050045 0.8950913917392676 >10 0.8275589622058886 10.03474743851817 >50 0.07087718374465096 3.148218756625957 >100 0.039114077208427876 4.462963098871857 >500 0.0011175440329982149 0.48756772429499695 >1k 0.0011175440329982149 1.458559792999979 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3914 0.3483353061061025 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 2922 0.2600500164644945 No Hit TATCAACGCAGAGTACTTTTTTTTT 2511 0.22347213940532026 No Hit GGTATCAACGCAGAGTACTTTTTTT 1775 0.15797015031638528 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1561 0.13892473501063518 No Hit GTGGTATCAACGCAGAGTACATGGG 1199 0.10670772407287096 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1193 0.10617374046616769 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 8.899726778387903E-5 0.0 0.0 0.0 0.0 4 8.899726778387903E-5 0.0 0.0 0.0 0.0 5 8.899726778387903E-5 0.0 0.0 0.0 0.0 6 8.899726778387903E-5 0.0 0.0 0.0 0.0 7 8.899726778387903E-5 0.0 0.0 0.0 0.0 8 8.899726778387903E-5 0.0 0.0 0.0 0.0 9 8.899726778387903E-5 0.0 0.0 8.899726778387903E-5 0.0 10 8.899726778387903E-5 0.0 0.0 8.899726778387903E-5 0.0 11 8.899726778387903E-5 0.0 0.0 8.899726778387903E-5 0.0 12 8.899726778387903E-5 0.0 0.0 8.899726778387903E-5 0.0 13 8.899726778387903E-5 0.0 0.0 8.899726778387903E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGCCA 40 2.7590632E-4 16.625938 10 TTGCGCG 230 0.0 14.053686 18 TTTGCGC 245 0.0 13.581294 17 TACACCG 50 0.0014944228 13.305506 5 TCTCGAC 50 0.0015116183 13.285321 12 TATAACG 65 5.4904765E-5 13.143434 2 TGCGCGC 260 0.0 12.431552 19 CGAGCCT 70 1.0861707E-4 12.21934 4 CGTGACA 55 0.0030498512 12.10024 14 TAACGCA 110 3.787318E-8 12.095914 4 TATCACG 55 0.0030857725 12.081338 2 CTCGACG 55 0.003093 12.077564 13 AGGACCG 95 1.0337171E-6 12.004968 5 ATTTGCG 280 0.0 11.883632 16 GGTATCA 1335 0.0 11.732278 1 GTGTTAC 65 8.0738077E-4 11.683052 1 ACGGCCC 90 7.3951633E-6 11.619552 15 CGAGGTT 90 7.4209656E-6 11.615917 4 AACTGGC 180 3.6379788E-12 11.091391 17 TAAGGTA 60 0.005855852 11.087921 4 >>END_MODULE